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EBI DbfetchID X78413; SV 1; linear; genomic DNA; STD; PRO; 4418 BP. XX AC X78413; XX DT 23-FEB-1995 (Rel. 42, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 3) XX DE L.lactis tyrA, aroA, aroK and pheA genes. XX KW 5-enolpyruvylshikimate-3-phosphate synthase; aroA gene; aroK gene; KW pheA gene; prephenate dehydratase; prephenate dehydrogenase; KW shikimate kinase; tyrA gene. XX OS Lactococcus lactis OC Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus. XX RN [1] RX DOI; 10.1007/BF00290140 RX PUBMED; 7823907. RA Griffin H.G., Gasson M.J.; RT "Genetic aspects of aromatic amino acid biosynthesis in Lactococcus RT lactis"; RL Mol. Gen. Genet. 246(1):119-127(1995). XX RN [2] RP 1-4418 RA Griffin H.G.; RT ; RL Submitted (06-JUN-1994) to the EMBL/GenBank/DDBJ databases. RL H.G. Griffin, Institute of Food Research, Norwich Research Park, Colney, RL Norwich NR4 7UA, UK XX FH Key Location/Qualifiers FH FT source 1..4418 FT /organism="Lactococcus lactis" FT /strain="FI5876" FT /mol_type="genomic DNA" FT /db_xref="taxon:1358" FT -35_signal 19..24 FT -35_signal 25..30 FT -10_signal 41..46 FT -10_signal 47..52 FT RBS 91..96 FT CDS 105..1169 FT /transl_table=11 FT /gene="tyrA" FT /product="prephenate dehydrogenase" FT /EC_number="1.3.1.12" FT /db_xref="GOA:P43901" FT /db_xref="InterPro:IPR003099" FT /db_xref="UniProtKB/Swiss-Prot:P43901" FT /protein_id="CAA55179.1" FT /translation="MKKILIIGLGLIGSSIALGIKKAHPEFEILGSDREEVENIAQKRG FT IIDSKVELVKGAQEADIIILAVPISVTLELLKQIATFDLKDGLLITDAGSTKSEIVELA FT NQLFSGTKVKFIGGHPMAGSHKSGVMAADLNLFENAYYVLTEESQELRELLKGLHAKFI FT ILDAKEHDKVTGQVSHFPHILASTLVWQSDDYAKEHPLVKHLAAGGFRDLTRIAEADSL FT MWTSVLLSNPEITLERIENFKKHLDEIALKITKRDSQAIEHFFEEGKKIRQAMEIHKGA FT LPNFYDLFISVPDEKGVVLRVLALLQDFSITNIKINEENREDIHGQLQISFKRAEDLQE FT AREIIEKATDFTVV" FT terminator 1226..1252 FT RBS 1279..1282 FT CDS 1289..2581 FT /transl_table=11 FT /gene="aroA" FT /product="5-enolpyruvylshikimate-3-phosphate synthase" FT /EC_number="2.5.1.19" FT /db_xref="GOA:P43905" FT /db_xref="InterPro:IPR006264" FT /db_xref="UniProtKB/Swiss-Prot:P43905" FT /protein_id="CAA55180.1" FT /translation="MKLKINSQGLKGRLKVPGDKSISHRSIMFGSIAKGKTIIHDILRG FT EDVLSTIEAFRALGVEIEDDGQVITVHGQGISKLKEPEKALDMGNSGTSTRLLSGILAG FT LPFEATLFGDDSLSKRPMDRVATPLQMMGAEIVGQTDKVKLPMTIKGSAHLKAIDYILP FT VASAQVKSAVIFAALQAEGLTKVVEKEKTRSHTEEMLVQFGGEITVSDKTILVPGGQKL FT LGQEVTVPGDISSAAFWLVAGLVVENSGLILENVGINETRTGILEVIQAMGGQLEILEQ FT DEVAKAATLKVKASQLKGTEISGDLIPRLIDELPIIALLATQAEGKTIIRDAAELKVKE FT TDRIAVVADALNSMGANIEPTDDGMIIQGGTKLHAPENAINTLGDHRIGMMVAIAALLV FT ENGEIELERAEAIQTSYPSFFDDLEKLSGNL" FT RBS 2593..2596 FT CDS 2605..3093 FT /transl_table=11 FT /gene="aroK" FT /product="shikimate kinase" FT /EC_number="2.7.1.71" FT /db_xref="GOA:P43906" FT /db_xref="HSSP:1L4U" FT /db_xref="InterPro:IPR000623" FT /db_xref="UniProtKB/Swiss-Prot:P43906" FT /protein_id="CAA55181.1" FT /translation="MSIILIGFMGAGKSTVAKLLAEEFTDLDKLIEEEIEMPIATFFEL FT FGEADFRKIENEVFELAVQKDIIIATGGGIIENPKNLNVLDRASRVVFLTADFDTLWKR FT ISMDWQNVRPLAQDKEAAQLLFEKRMKDYSLVADLTIDVTDKSPEQIAEQIREKWEIE" FT RBS 3081..3084 FT RBS 3088..3090 FT CDS 3095..3934 FT /transl_table=11 FT /gene="pheA" FT /product="prephenate dehydratase" FT /EC_number="4.2.1.51" FT /db_xref="GOA:P43909" FT /db_xref="InterPro:IPR018528" FT /db_xref="UniProtKB/Swiss-Prot:P43909" FT /protein_id="CAA55182.1" FT /translation="MKIAYLGPRGSFCSVVAEAAFKSEELYSYATILDVIEAYNEGECD FT FALVPIENSTEGTVNMSIDKIFHDSNAKVVAEFVLPISQNLLAVSKEQKIEHIYSHPQA FT LAQTRVYLRKFYPQAQVEITESTSAAAEFVKNNPDLPAAAVANSFAAKMYDLEFIAENI FT QDLAGNSTRFWLLGKEKQSFDLNQTKDKVTLALTLPDNLPGALHKAISVFAWRDIDMTK FT IESRPLRTRLGQYFFIIDLENNATNSLKIPYALEELAGLGVNVRLLGNYSVYSLGEV" XX SQ Sequence 4418 BP; 1434 A; 649 C; 988 G; 1347 T; 0 other; gactctatct agcaaaactt attttggata aactagaatt tcaaattgca ataagttctg 60 aaattggtga gggaacttgt gtgatgatca agaaagagta aagaatgaaa aagatattaa 120 ttataggtct cggactgatt ggaagttcta tagccctagg cataaaaaaa gcccatcctg 180 agtttgaaat tcttggttcg gaccgtgaag aagttgaaaa tattgctcaa aaacgtggaa 240 ttattgattc taaggtcgaa ttagttaaag gagcccaaga agcagatatt attattttag 300 ctgtgccaat ttcagtgact ctcgagcttt taaagcaaat tgcaacgttt gatttaaaag 360 atggactttt gattactgat gctggttcaa ccaagagtga gattgtcgag ctagctaatc 420 aacttttttc tgggacaaaa gtaaaattta tcggagggca tccgatggct ggctctcata 480 aatctggcgt aatggcggca gatcttaatt tatttgaaaa tgcttattat gttttgacag 540 aagaaagtca agaattaaga gagttactta agggacttca tgctaaattt attattttag 600 atgccaagga acatgataaa gtaacgggtc aagtgtctca ttttccacat attttggctt 660 caactctggt ctggcaatca gacgattatg ctaaagagca tcctttagtc aaacatttag 720 ctgctggagg ttttcgtgat ttaacgagaa tcgctgaggc tgacagctta atgtggacta 780 gtgtcttact ttctaatccg gaaattacat tagagagaat tgagaatttt aaaaaacact 840 tagatgaaat tgctttaaaa attacaaaaa gagattccca agctattgaa cacttttttg 900 aagaaggtaa aaagattcga caagcaatgg aaattcataa aggggcactg ccaaactttt 960 atgatttgtt tatctcggtt cccgatgaaa aaggagttgt tttacgagtt ttagcactct 1020 tacaggattt ttcgattaca aatatcaaaa ttaatgaaga aaatcgtgaa gatattcatg 1080 gtcaactgca aatctctttt aaacgagcag aagatttgca agaggcgcgt gaaattattg 1140 aaaaagcaac tgattttaca gttgtttgat aaaggttaaa gaactgacat tcttagtctt 1200 aaaatcttag gaagaaatga taaaaataag agggccttga gcgccctttt atgctataat 1260 aaatactaaa aaatgtttag gaaataaaat gaaattaaaa ataaattcac aagggttaaa 1320 agggcgttta aaggttccag gagataaatc aatttcacat cgaagcatta tgtttggctc 1380 aattgctaaa ggaaaaacaa tcattcatga tattttgcgt ggcgaagatg ttttatctac 1440 cattgaagcc tttcgagctt taggagttga gattgaggac gatggtcaag ttattactgt 1500 ccatggtcag gggatttcaa aacttaagga gccagaaaaa gcacttgata tgggaaattc 1560 tggaacttca acccgcctgt tgtcggggat tttagcagga cttccttttg aagcaacttt 1620 gtttggggat gatagtttat ccaaaagacc aatggaccgt gtcgcaactc cgcttcaaat 1680 gatgggggct gaaatagttg gacaaactga taaggttaaa ttaccgatga caatcaaagg 1740 aagtgctcat ttaaaagcaa tcgattatat ccttccggtc gcttcggccc aagtaaaatc 1800 ggcggtaatt tttgctgctt tacaagcaga aggactgaca aaagttgttg agaaggaaaa 1860 aacacgctca catacagaag aaatgttagt tcaatttggc ggagaaatta ctgtttctga 1920 taaaaccatt cttgttcctg gcgggcaaaa acttttgggg caagaggtga cagttcctgg 1980 tgacattagt tcagcagcct tttggcttgt agctggctta gttgttgaaa attcaggact 2040 tatcttggaa aatgttggaa ttaatgaaac aagaacgggt attttagaag tcattcaggc 2100 aatgggtggc cagttggaaa ttttagagca agatgaggtg gcaaaagccg cgactctaaa 2160 agtaaaagcg agtcaactta agggaactga aatatcgggt gatttaattc caagattaat 2220 tgatgaatta ccaattattg cgcttttggc tacacaagcc gaaggtaaaa caattattcg 2280 cgatgcggct gaacttaaag ttaaggaaac cgaccggatt gctgttgtgg ctgatgcact 2340 caatagtatg ggtgcaaata ttgaaccaac agatgatgga atgattattc aaggtggaac 2400 aaaattacat gctccagaaa atgcaattaa tactttaggt gatcacagaa ttgggatgat 2460 ggttgcaatt gctgcacttt tggttgaaaa tggtgaaatt gagcttgaac gagctgaggc 2520 cattcaaacg agctatccaa gtttctttga tgatttagaa aaattgtcag gaaatctttg 2580 aaattaaatg ataggaagta aaaaatgagt atcattttaa ttggatttat gggtgccgga 2640 aaatcaacgg tggcaaaact tttagcagaa gaatttactg atttagataa gttgattgaa 2700 gaagagattg aaatgccaat tgcgactttt tttgaattat ttggagaagc tgattttcgt 2760 aaaattgaaa atgaggtttt tgagcttgct gttcaaaaag atattattat cgcaactggt 2820 ggaggaatca tagaaaatcc aaagaattta aatgttcttg atagagcatc acgagttgta 2880 tttttgactg ctgattttga tactttatgg aagcgaattt ccatggattg gcagaatgtt 2940 cgacctttag ctcaagacaa agaagctgct caacttttat ttgaaaaaag aatgaaagac 3000 tattcattgg ttgcggattt aacaattgat gtgaccgata aaagtccaga gcagattgca 3060 gagcagatta gagaaaagtg ggagatagag taggatgaaa attgcatatt taggtccacg 3120 aggttctttt tgctcagtcg tagccgaagc agcttttaaa tctgaggagt tgtactctta 3180 tgcaactatt ttggatgtca ttgaagccta taatgagggc gaatgtgatt ttgcactagt 3240 tcctattgaa aattcgactg aaggaacggt caatatgagc attgataaaa tttttcatga 3300 ttcaaatgcc aaggttgtgg ccgagtttgt tctcccaatt tctcaaaatt tattggcagt 3360 ttctaaagaa caaaaaattg agcatattta ctctcatcct caagctttgg ctcaaacgag 3420 agtttatttg agaaaatttt atcctcaggc gcaggtggaa attactgagt ccacttcggc 3480 agcggctgag tttgtgaaaa ataatccgga cttgcccgca gcagcggtgg caaattcttt 3540 tgcagctaaa atgtatgatt tggaattcat tgctgaaaat attcaagatt tggcggggaa 3600 ttcgactcgt ttttggttac taggaaaaga aaagcaatcc tttgatttaa atcaaacaaa 3660 ggataaggtg actttggctt tgacgctacc tgacaatttg ccaggagcat tgcacaaagc 3720 aatttctgtt tttgcttggc gtgatattga catgactaaa attgagtcac gtcctttaag 3780 gacacgtttg ggacagtact tttttattat tgatttagaa aataatgcaa cgaacagttt 3840 aaagattcct tatgctcttg aagaattggc aggtttggga gtaaatgttc gattgttagg 3900 aaattattca gtatattcat taggtgaggt gtaaaggtga gaatttattt tgtacgacat 3960 ggtaaaacgg agtggaattt ggcacggcgt ttgcagggac aaaagggaga ctcaccttta 4020 ttgccagaat cttatgaggc aattaaacgc gttcataagt ttttagaacc gattgaattt 4080 gacaaggttt tatcaagccc tcaaaaaaga gctttgacaa cagcagattt actgaccaat 4140 cagtctgtaa gtactgacaa gagattatca gaatggaatt ttggtgaatt agagggttgg 4200 ttcattcgtg atgcaatcgc ctaagtaccc taaggaaatg catgattctc gctttgaatt 4260 agatcaattt gatggaagtg cttttggggc tgaatcagtt acttcagttt tggcacgctt 4320 tgattctttg gccggggact tattgaattc taaaatggac aatgtacttt tagttggtca 4380 cggagcttct gggacggctg gaatgcgaca tttggctg 4418 // |
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