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EBI DbfetchID X77617; SV 1; linear; genomic DNA; STD; PRO; 8025 BP. XX AC X77617; XX DT 01-JUN-1994 (Rel. 39, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 32) XX DE E.coli kfiD, kfiC, kfiB and kfiA genes XX KW glycosyltransferase; kfiA gene; kfiB gene; kfiC gene; kfiD gene; KW UDP-glucose dehydrogenase. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RX DOI; 10.1111/j.1365-2958.1995.mmi_17040611.x RX PUBMED; 8801416. RA Petit C., Rigg G., Pazzani C., Smith A., Sieberth V., Stevens M., RA Boulnois G., Jann K., Roberts I.S.; RT "Region 2 of the Escherichia coli K5 capsule gene cluster encoding proteins RT for the biosynthesis of the K5 polysaccharide"; RL Mol. Microbiol. 17(4):611-620(1995). XX RN [2] RP 1-8025 RA Roberts I.S.; RT ; RL Submitted (08-FEB-1994) to the EMBL/GenBank/DDBJ databases. RL I.S. Roberts, University of Leicester, Dept of Microbiology, PO Box 138, RL Medical Sciences Building, University Road, Leicester LE1 9HN, UK XX FH Key Location/Qualifiers FH FT source 1..8025 FT /organism="Escherichia coli" FT /strain="K5" FT /isolate="2980" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT CDS 3705..4007 FT /transl_table=11 FT /gene="hypothetical ORF" FT /db_xref="UniProtKB/TrEMBL:Q47328" FT /protein_id="CAA54707.1" FT /translation="MGELRCYVITMIDEHSNYALAQAVPSLNCDIIKLFFCRADRLFPI FT DINQGIADNGKEFPGNFDKAPQESASKPYGPTPLRLERTSERFKRTPKEQFIEFK" FT CDS complement(227..1405) FT /transl_table=11 FT /gene="KfiD" FT /product="UDP-glucose dehydrogenase" FT /db_xref="GOA:Q47329" FT /db_xref="HSSP:1DLI" FT /db_xref="InterPro:IPR001732" FT /db_xref="UniProtKB/Swiss-Prot:Q47329" FT /protein_id="CAA54708.1" FT /translation="MFGTLKITVSGAGYVGLSNGILMAQNHEVVAFDTHQKKVDLLNDK FT LSPIEDKEIENYLSTKILNFRATTNKYEAYKNANYVIIATPTNYDPGSNYFDTSSVEAV FT IRDVTEINPNAIMVVKSTVPVGFTKTIKEHLGINNIIFSPEFLREGRALYDNLHPSRII FT IGECSERAERLAVLFQEGAIKQNIPVLFTDSTEAEAIKLFSNTYLAMRVAFFNELDSYA FT ESFGLNTRQIIDGVCLDPRIGNYYNNPSFGYGGYCLPKDTKQLLANYQSVPNKLISAIV FT DANRTRKDFITNVILKHRPQVVGVYRLIMKSGSDNFRDSSILGIIKRIKKKGVKVIIYE FT PLISGDTFFNSPLERELAIFKGKADIIITNRMSEELNDVVDKVYSRDLFKCD" FT CDS complement(1447..3009) FT /transl_table=11 FT /gene="KfiC" FT /product="putative glycosyltransferase" FT /db_xref="GOA:Q47330" FT /db_xref="HSSP:1QGQ" FT /db_xref="InterPro:IPR001173" FT /db_xref="UniProtKB/TrEMBL:Q47330" FT /protein_id="CAA54709.1" FT /translation="MNAEYINLVERKKKLGTNIGALDFLLSIHKEKVDLQHKNSPLKGN FT DNLIHKRINEYDNVLELSKNVSAQNSGNEFSYLLGYADSLRKVGMLDTYIKIVCYLTIQ FT SRYFKNGERVKLFEHISNALRYSRSDFLINLIFERYIEYINHLKLSPKQKDFYFCTKFS FT KFHDYTKNGYKYLAFDNQADAGYGLTLLLNANDDMQDSYNLLPEQELFICNAVIDNMNI FT YRSQFNKCLRKYDLSEITDIYPNKIILQGIKFDKKKNVYGKDLVSIIMSVFNSEDTIAY FT SLHSLLNQTYENIEILVCDDCSSDKSLEIIKSIAYSSSRVKVYSSRKNQGPYNIRNELI FT KKAHGNFITFQDADDLSHPERIQRQVEVLRNNKAVICMANWIRVASNGKIQFFYDDKAT FT RMSVVSSMIKKDIFATVGGYRQSLIGADTEFYETVIMRYGRESIVRLLQPLILGLWGDS FT GLTRNKGTEALPDGYISQSRREYSDIAARQRVLGKSIVSDKDVRGLLSRYGLFKDVSGI FT IEQ" FT CDS complement(3342..3614) FT /transl_table=11 FT /gene="hypothetical ORF" FT /db_xref="UniProtKB/TrEMBL:Q47331" FT /protein_id="CAA54710.1" FT /translation="MNSVFPWGGSHIGISRILSCAAVNILPMVEMLGQTRRCCSLHGFK FT QTKICSSRLGSSILSRRISYRITRCHCFVRCTSGALLREIGASGP" FT CDS complement(6424..7140) FT /transl_table=11 FT /gene="KfiA" FT /db_xref="UniProtKB/TrEMBL:Q47332" FT /protein_id="CAA54711.1" FT /translation="MIVANMSSYPPRKKELVHSIQSLHAQVDKINLCLNEFEEIPEELD FT GFSKLNPVIPDKDYKDVGKFIFPCAKNDMIVLTDDDIIYPPDYVEKMLNFYNSFAIFNC FT IVGIHGCIYIDAFDGDQSKRKVFSFTQGLLRPRVVNQLGTGTVFLKADQLPSLKYMDGS FT QRFVDVRFSRYMLENEIGMICVPREKNWLREVSSGSMEGLWNTFTKKWPLDIIKETQAI FT AGYSKLNLELVYNVEG" FT mRNA 4301..5992 FT /gene="KfiB" FT repeat_region 4711..4795 FT repeat_region 4796..4879 XX SQ Sequence 8025 BP; 2645 A; 1491 C; 1183 G; 2706 T; 0 other; ctttggatat agcacataat atacatttcc aacgcacaag ccaagtattc taattaacat 60 tagagttatg aaaaattcga tataaacaca acagcagggc aagaaattta accaacatct 120 tattttacga tcgaggatta ataaagaaca taccaacgag gcatattttt aacggattaa 180 ataatgcatt tccttctctt aatagttaat agtatataac aatacattag tcacatttaa 240 acaaatcgcg actatagact ttgtcgacca catcgttcaa ctcctctgac attcggttag 300 tgataataat atcagctttc cctttaaaga tcgccagctc ccgttccaaa ggtgagttaa 360 agaatgtatc tccagaaata agcggctcat aaataattac tttcacgcct tttttcttga 420 tacgctttat aataccaaga atagaagaat ctctaaaatt atctgaacca cttttcataa 480 tcaaacgata aacccccaca acttgtggtc tatgtttcaa aataacatta gtgataaagt 540 ccttacgtgt acggttagca tcaacaattg cagatataag tttattcgga acagactgat 600 agttggctaa taattgcttg gtatcttttg gcaaacagta gccaccataa ccaaaagaag 660 gattattgta gtaattacca atgcgcggat ccaaacaaac accgtcaata atctgacgcg 720 tattcagacc aaaactttct gcgtaactat ccaattcatt aaaaaatgca actcgcatag 780 ccaaataagt atttgaaaat aacttaatcg cttccgcttc cgtagaatct gtaaataaaa 840 cgggtatatt ttgtttaatc gctccttcct gaaataacac tgccaaacgt tctgcccgtt 900 cagaacattc accgataata atgcgagatg gatggagatt atcgtatagg gctcttcctt 960 ctcgtaaaaa ttctggagag aagataatat tattaatacc taaatgttct ttaattgttt 1020 ttgtgaaacc tactgggacc gtagatttaa ccaccataat tgcgtttggg ttgatttccg 1080 ttacgtcacg aatgacagct tcaacgcttg atgtatcaaa gtaatttgaa cctgggtcat 1140 aattcgttgg tgtagcaata ataacgtaat tggcattttt ataggcttca tatttgttag 1200 tagttgcgcg aaaattaagt attttagttg aaagataatt ttcaatttcc ttatcctcta 1260 taggagagag tttatcatta agtaagtcaa cttttttttg atgggtatca aatgcaacca 1320 cttcatgatt ttgagccatt agaattccat ttgaaagccc aacgtaacca gcgcctgaaa 1380 cagttatttt tagtgttccg aacattttat atagccccaa aaatttaata tatatagaat 1440 aacaaactat tgttcaatta ttcctgatac atctttaaac aaaccatagc gagataataa 1500 accacgtaca tctttatcac ttacgatact tttccctaac actcgttgtc ttgccgcgat 1560 atcactatat tctcttcgag attgtgatat atatccatca ggtagagctt ctgttccttt 1620 attcctggta agtccggagt ctccccataa ccccaatatc aatggctgca gtaatcttac 1680 aatactttct cgcccataac gcattattac tgtttcataa aactccgtat ctgcaccaat 1740 taaagattgt ctatagccac caactgtcgc aaaaatatct ttttttatca ttgacgatac 1800 aacagacatt cttgtggctt tatcatcata gaagaattga atttttccat ttgacgcaac 1860 acggatccag ttagccatac agattacagc cttattattg cgaagaacct caacttgtct 1920 ttgtattctc tccggatgag aaagatcatc tgcatcttga aaggtgatga aattaccgtg 1980 tgcttttttt attagctcat ttcttatatt ataagggcct tggttttttc gtgagctata 2040 tactttcact cttgaactag aataagctat gctcttaatt atttcaaggc ttttgtccga 2100 tgaacaatca tcgcacacga gaatttcaat attttcatat gtttgattca acaatgaatg 2160 taatgagtat gcaatagtat cttctgaatt gaatactgac attattatac taacaagatc 2220 ttttccataa acattttttt tcttatcgaa cttaattcct tgcaatataa ttttatttgg 2280 gtatatatca gttatttctg ataaatcgta ttttcgtaga catttgttaa attgactcct 2340 ataaatattc atattatcta ttacagcatt acaaataaaa agttcttgct cagggagtag 2400 attataacta tcttgcatat catcgtttgc atttaataat aaagtcaggc cataccctgc 2460 atcggcttga ttatcaaatg ctaaatattt atatccattt ttagtataat catgaaattt 2520 tgaaaacttc gtacaaaaat aaaaatcttt ttgtttgggc gacaatttta gatggtttat 2580 atattcgata tatcgttcaa aaataagatt aatgagaaaa tcactccttg aataccgtag 2640 agcgttactt atatgttcaa aaagcttaac tcgttcgcca tttttaaaat aacgagattg 2700 aattgttaga taacaaacaa ttttaatata agtatccaac ataccaactt ttctaagaga 2760 atctgcatat cccaataaat aagaaaactc attgccagaa ttctgagctg atacattctt 2820 agatagttca agtacattgt cgtattcgtt tattcttttg tgaataaggt tatcgttacc 2880 ttttaaaggc gagtttttat gttgaagatc aactttctcc ttatgaattg ataataaaaa 2940 atcaagagca ccaatatttg tccctaattt ctttttacgt tcaactaaat ttatatattc 3000 tgcgttcata cattcactta atgataaata aaatgagaat aaaactgcat ttactaaata 3060 ttataaatat aaatctttaa aaacatcact aaaactcaac gaatgtcaca gtgtaagctc 3120 acagagatat tgcaattatc tccggacaag cgagggaaat gctaagttga tgcaacgtat 3180 ctgcggcgga tatttgaggc attaccatgc caacatctat gtgatgaaac actttgttat 3240 acctgagatt acaaaattaa ggatgaaaaa atatacttct gggagaaaca tgagatcaat 3300 acagttgcgg aagtttttgc tgtatccaca cgaacgctgt attatggccc agaggcacct 3360 atttcgcgta gcaaagctcc gctagtacat cgcacaaagc aatggcatct cgtaattctg 3420 taagaaatac gtcgactgag aatagagctt cccaacctag aagaacagat ttttgtttgc 3480 ttgaagccgt ggagtgaaca gcaccgcctg gtctgtccga gcatttcaac catcggcagg 3540 atgttcacgg ccgcacacga taaaatgcga ctgataccga tgtgactgcc tccccaagga 3600 aaaacactgt tcataaaaaa tacaatcctc caacccgcag atcaaaacgc tatcgcccgg 3660 taaagagcag tgagctcgtt ggtatagatg ctattgagct cagaatgggc gagctgcgct 3720 gttatgtcat taccatgatc gacgaacaca gcaactacgc tctggcacag gccgtgccgt 3780 cacttaactg tgatatcatc aaactcttct tttgccgtgc agaccggcta ttccctatcg 3840 atatcaacca gggcatcgca gataacggga aagagttccc aggaaacttc gacaaagcac 3900 cgcaggaatc cgctagcaag ccctatggac ctaccccttt acgcctagaa cgcacctctg 3960 aacgttttaa gcgaacgccg aaagaacagt tcattgaatt taaataaata gactctttaa 4020 agagctaaac atatttaatc agaaactagc tgaaaaccta gtactatact gcagtaaaag 4080 acagcatgaa gtgctcgaat tactgacacc agaagaaaat attttacgag agaacaaaat 4140 ttgcaatatg tggcgaatct atacggtcac ttgcaaaagt ataaattggt attataatgc 4200 gtgatattat ataaataaaa taactaacta ttttaacgaa agcaaaacac cacaaagaga 4260 aaaataaaat ttgcaatata tctcattagg gttcacattt ttaacagccc ttgattttag 4320 ctctcctata tttataaaca acaacaatta acttaaatgg aagggtaatg aatccaaata 4380 tcgaattagc actggagata atagcctgcc ctaattgata agataaatgt ttcttaactc 4440 tttcagcctt atcagcatca gcatataaat agagtggcac atcaacgcca taactcttct 4500 tacgtttttt ttcaaaaaca cactcacgga taagtgcaaa tggcatggta ataatcccct 4560 tgagggattt agaatacgag actataatat aaccaagtcg ataacctaac tcttgtttta 4620 tctgctcagc tgctccatat agcacaggtg gaagtttctt tttaatttcc tgattttcta 4680 taaaatagtg ttccaactgc tcttgcacta tatgcaattg ttttaaaatc tgttcttttt 4740 ctgatattgc ctgagtgagt tttgattctt tttcgcttat ctcttcttca tatttatgca 4800 attgttttaa aatctgttct ttttctgata ttgcctgagt gagttttgat tctttttcgc 4860 ttatctcttc ttcatatttc tgtatgtcat ttattttttt ttcaatatca tctggatatt 4920 tttcttgtct cacaacatcg ttgtgattta tttttgaaag tttaattacc tcttcctttt 4980 ctttttttag ctcattaatc ctgttcaata ataaaaattg tcttttctta aatatagatt 5040 tagtcaattc aatatctgca tccttctttt ttgactcttg caccaaatga gatacaatat 5100 tgaagataga atttctctct tcgactacct cccataatgc agcctcagcc gaaacgttat 5160 ctttcgatgt ttctatataa ggtaggtttg cataggcttg taactcatta taaagcttca 5220 gaacctcggt tctttctttt attaattcag atactaagta ctcactaacc tttgtatcat 5280 taaatgtaat ttcagtctca tttgcactta aagaaaataa agttgtatat ttttcaagca 5340 gggcaatttc atttgatttt gtataatcat tattctcaac cacatcaaac aatattgaat 5400 ttttgtaatg cgcagacatt agattatcta tttttataat attactcaat ggctttataa 5460 tatttttctt attgctttga aacgcctcaa aattaatcaa cacacatcgt tctttattct 5520 ccaaaaaaaa atcaagcaat cttttattat atgcaatcca gttatctaca ctattattta 5580 tatttaaaga gagaggggca ttatttactg aacgcattaa attatattta gggtgatcat 5640 atatcatgat gaattttatg cttttatcta tatttttcca aaaatcaaga aaaaatatta 5700 tagatggatc tgcccacccc caaagtttgt tatttagatt gttcatcatt agttcaagaa 5760 ccatgctatc ccacatggtt gagacttgaa cagctgagaa ttctctctca tctactacat 5820 cattaacttc tggtgtttga taaaattgac ataatgaagc ggtgatatat tctggggaaa 5880 ttttattact tgtagaataa tttggtgagt tcatgccaat ttctttcaaa aaatgttcaa 5940 ctatctgata ctttgagcca ggatgtccga ctagcactaa tttattcatc atatttcctc 6000 tttattatct acaagattac tttattttaa acactgtcgt ttatcgtaat ggtgatattg 6060 cagacatata tttcaaatct gtaatgtaat ttaggtggcg ttatgtgata atactaaatg 6120 aaactatcta tatctcaata gcacatctga tttatttggt atagccattc atcaataaac 6180 aatatcaata taactcagag cacctcctaa taattgacat attgcgttac caccttcttt 6240 tagacaaaaa tcttaattat aaactctacc aaactaacgc tgatattttt caaaatcata 6300 aaacaaatgg tttaaataag tatataacaa aatatcaacg tacaactatt taaaacaagt 6360 atttatacta taaacttcag aaaccaacac aaaatacagg aatgtgaata aaaaagtaag 6420 tttttaccct tccacattat acactaattc gaggttaagt tttgaatatc ctgcgattgc 6480 ttgtgtttct tttatgatgt ctaaaggcca tttttttgta aatgtgttcc aaagtccttc 6540 cattgaacct gatgagacct ctcttagcca gtttttttct ctgggaacac atatcatacc 6600 aatttcattc tctaacatat agcgagaaaa tctaacatcg acgaatcgtt gagaaccatc 6660 catatatttt aaagatggta attgatctgc cttaagaaaa acagtccctg tacctaattg 6720 atttacaact ctcggtcgca atagcccttg agtaaatgaa aatacttttc ttttagactg 6780 atctccatca aatgcatcta tgtatataca gccatgaatc ccaacaatgc aattgaatat 6840 tgcaaaggaa ttataaaaat tgagcatttt ttctacataa tcgggagggt aaataatatc 6900 atcatctgta agtacgatca tatcattttt agcgcaagga aatataaatt tgcccacatc 6960 cttataatct ttatctggaa taactggatt taattttgaa aaaccatcta attcctcagg 7020 aatttcttca aactcattca ggcaaagatt aattttatct acttgagcat gtaaactttg 7080 tatagaatgc accaactctt tttttcgagg tgggtatgat gacatatttg caacaatcat 7140 ggggtaatat tttaattaat attgcatgtt aatgaaaact atcaacgtat tgatagtcgt 7200 ttatttatag gaaatgctta ataaacagaa cacattaagt tgattagaat gactccgcac 7260 ctcatggacg atatgtttaa ttaaaaataa ctactagggt gtacgtttga tatatttatc 7320 ttcatctaaa ttattactct taccatatca aaccactctt tgcataaaca tttcctgtaa 7380 ctatctaaaa ataaattggc agataattaa ttaaagaata acagcaacgt attacgagat 7440 cacgatcaaa ttcataatta cctagttcaa agtatactgt ccatcctcct catacactaa 7500 atacaataga gtttatataa ttatatagag tgtgattaca atctccggca atgacactgt 7560 tagtgcaaca gtaatcaggc ctccctctta gtatcgctta cgctgaaaga ataactggca 7620 gcctgccaca ccatgttgtc tggcaacgag gaagaccgcc attttcggtt caaggctctg 7680 ctgaaaaata gcgattttcc tgtgtggcac gctgtctgcg tttctctgct tctaagacac 7740 caattatcta ttctccaact actagtctaa aagactagta tcactactat cagttattta 7800 aatgatattg attgcctaga gattcaggga gctagtctag ttattcagag aacggtatct 7860 taacaatttt gttatgattt taggagatct ggatttctat tcatgatgct tggcgacaaa 7920 aataaatgaa caaaaagaac ttatatcagc cacagcaaca taatttctta tgtcacttgt 7980 attctacctg tgtcattaag tttatcgttt caatttcata agata 8025 // |
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