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EBI DbfetchID X74289; SV 1; linear; genomic DNA; STD; PRO; 2319 BP. XX AC X74289; XX DT 28-DEC-1993 (Rel. 38, Created) DT 12-JUN-2006 (Rel. 88, Last updated, Version 5) XX DE B.stearothermophilus ama gene for N-acyl-L-amino acid amidohydrolase XX KW ama gene; N-acyl-L-amino acid amidohydrolase. XX OS Geobacillus stearothermophilus OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Geobacillus. XX RN [1] RP 1-2319 RA Charlier D.R.; RT ; RL Submitted (23-JUL-1993) to the EMBL/GenBank/DDBJ databases. RL D.R. Charlier, Research Insitute Ceria/Coovi, E. Grysonlaan 1, 1070 RL Brussels, BELGIUM XX RN [2] RP 1-2319 RX PUBMED; 8285691. RA Sakanyan V., Desmarez L., Legrain C., Charlier D., Mett I., Kochikyan A., RA Savchenko A., Boyen A., Falmagne P., Pierard A., et a.l.; RT "Gene cloning, sequence analysis, purification, and characterization of a RT thermostable aminoacylase from Bacillus stearothermophilus"; RL Appl. Environ. Microbiol. 59(11):3878-3888(1993). XX CC Overlaps with Y08751 XX FH Key Location/Qualifiers FH FT source 1..2319 FT /organism="Geobacillus stearothermophilus" FT /strain="NCIB 8224" FT /mol_type="genomic DNA" FT /clone="pAVK66" FT /db_xref="taxon:1422" FT CDS <1..1053 FT /codon_start=1 FT /transl_table=11 FT /gene="ORF1" FT /db_xref="GOA:P37113" FT /db_xref="InterPro:IPR011650" FT /db_xref="UniProtKB/Swiss-Prot:P37113" FT /protein_id="CAA52341.1" FT /translation="DRERKEGTNPDATVVLVGSHLDSVYNGGCFDGPLGVLAGVEVVQT FT MNEHGVVTHHPIEVVAFTDEEGARFRFGMIGSRAMAGTLPPEALECRDAEGISLAEAMK FT QAGLDPDRLPQAARKPGTVKAYVELHIEQGRVLEEAGLPVGIVTGIAGLIWVKFTIAGP FT AEHAGATPMSLRRDPMAAAAQIIIVIEEEARRTGTTVGTVGQLHVYPGGINVIPERVEF FT VLDLRDLKAEVRDQVWKAIAVRAETIAKERNVRLTTERLQEMAPVLCSEVVKQAAERAC FT KQLGYPPFWLPSGAAHDGVQLAPICPIGMIFVRSQDGVSHSPAEWSTKEDCAVGAEVLY FT HTVWQLAQGE" FT CDS 1087..2199 FT /transl_table=11 FT /gene="ama" FT /product="N-acyl-L-amino acid amidohydrolase" FT /db_xref="GOA:P37112" FT /db_xref="InterPro:IPR011650" FT /db_xref="UniProtKB/Swiss-Prot:P37112" FT /protein_id="CAA52342.1" FT /translation="MTKEEIKRLVDEVKTDVIAWRRHLHAHPELSFQEEKTAQFVYETL FT QSFGHLELSRPTKTSVMARLIGQQPGRVVAIRADMDALPIQEENTFEFASKNPGVMHAC FT GHDGHTAMLLGTAKIFSQLRDDIRGEIRFLFQHAEELFPGGAEEMVQAGVMDGVDVVIG FT THLWSPLERGKIGIVYGPMMAAPDRFFIRIIGKGGHGAMPHQTIDAIAIGAQVVTNLQH FT IVSRYVDPLEPLVLSVTQFVAGTAHNVLPGEVEIQGTVRTFDETLRRTVPQWMERIVKG FT ITEAHGASYEFRFDYGYRPVINYDEGDPRHGGNGVRAVRRRGSGPLETEHGRRRFLRLF FT AKSARQLFLRRRGQCRKRHRLPAPPPALYD" XX SQ Sequence 2319 BP; 520 A; 596 C; 741 G; 462 T; 0 other; gatcgggagc ggaaagaagg gacgaatccg gatgccacgg tcgtccttgt tggatctcat 60 ctcgattcgg tttacaacgg cggctgcttt gatggaccgc tcggggtgtt ggccggcgtg 120 gaagtcgttc agacgatgaa cgagcacggt gttgtgacgc accacccaat tgaagtagtg 180 gcgttcactg acgaagaggg agcgcgcttt cgtttcggca tgatcggcag ccgcgccatg 240 gccggaacac tgccgccgga agcgctcgag tgccgcgacg cggaagggat ttccctcgct 300 gaagcgatga aacaggcggg gcttgacccg gaccgcttgc cgcaggcagc gcgaaaacca 360 ggaacggtga aagcctatgt cgaattgcat atcgaacaag gacgggtgct ggaggaggct 420 ggtcttccag ttggcatcgt cactggcatc gccggtctga tttgggtgaa atttaccatc 480 gccggcccgg cggaacatgc cggcgccacg ccgatgtcat tgcggcgcga cccgatggcg 540 gcggccgccc agatcatcat agtgatcgaa gaggaagcaa gacgaacagg gacaacggtc 600 ggtactgtag gacagttgca tgtatatccg ggcggtatta atgtcattcc ggaacgggtc 660 gaatttgtgc tcgatttgcg cgacttgaag gctgaggtgc gcgatcaagt atggaaagcc 720 atagccgtgc gggcagagac gatcgccaag gagcggaacg ttcgcctcac gaccgagcga 780 ctgcaagaaa tggcgccggt gttatgttcc gaggtggtga aacaggccgc ggaaagagcg 840 tgcaagcagc tcgggtatcc gccgttttgg ctgccgagcg gcgcagccca tgacggcgta 900 cagttggctc cgatttgccc gatcgggatg atttttgtcc gctcccaaga cggggtgagt 960 catagtccgg cggaatggag tactaaagaa gactgcgccg ttggagcaga ggtgctttat 1020 catacagtgt ggcaactggc ccaaggggaa taacgaaaaa gacaaacgaa cagggggaga 1080 ctatggatga caaaggaaga aatcaaacgg ctcgtcgatg aagtgaaaac ggacgtcatc 1140 gcctggcgcc gtcatttgca tgcccatccg gaattgtcgt tccaagaaga gaaaacagcg 1200 cagtttgtct atgagacgct gcaatcattc ggtcatcttg aactttcgcg gccgacgaaa 1260 acgagcgtca tggcgcggct cattggccaa cagccaggcc gggtcgtcgc cattcgcgct 1320 gatatggacg cattgccgat tcaagaggaa aacacgtttg agtttgcctc aaaaaaccca 1380 ggcgtgatgc atgcgtgcgg acatgacggc catacggcga tgcttctcgg gacggcgaaa 1440 attttctccc agctgcgcga tgacattcgc ggtgaaatcc gctttttgtt ccaacacgcg 1500 gaagaattgt tccccggcgg ggcggaggag atggtgcaag ccggcgtcat ggacggggtg 1560 gacgtcgtca tcggcactca cctttggtcg ccgcttgagc gcggaaaaat cggcattgtg 1620 tatgggccga tgatggccgc acccgaccgc tttttcatcc gcatcatcgg caaaggcggc 1680 catggagcga tgccgcacca aacgatcgat gcgatcgcca tcggagcgca agtcgtgacg 1740 aacttgcagc acattgtctc gcgctatgtc gacccgctcg agccgcttgt tctgtccgtg 1800 acgcaatttg tggcgggtac ggcgcataat gtcctgcctg gggaggtcga aatccaaggg 1860 acagtgcgca cgttcgatga gacgctgcgg cgcacggtgc cgcaatggat ggagcgcatt 1920 gtcaaaggga tcaccgaagc gcacggcgcc tcgtatgagt ttcgatttga ctacggctac 1980 cgcccggtca tcaactacga tgaaggtgac ccgcgtcatg gaggaaacgg cgtgcgagct 2040 gttcggcgaa gaggcagtgg cccgcttgaa accgaacatg ggcggcgaag atttctccgc 2100 ctttttgcaa aaagcgcccg gcagcttttt ctacgtcggc gcgggcaatg tagaaaaagg 2160 catcgtttac ccgcaccacc acccgcgctt tacgattgac gaagacgcgc ttgagattgg 2220 cgtgcaaatg tttgtcgcgg cgacgttgaa actgttggcg ggggcggaat agctgttttc 2280 gcttgacata tccaagattg tcagttcaaa cgtttgatc 2319 // |
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