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EBI DbfetchID X72831; SV 1; linear; genomic DNA; STD; PRO; 6352 BP. XX AC X72831; XX DT 18-MAY-1993 (Rel. 35, Created) DT 12-JUN-2006 (Rel. 88, Last updated, Version 7) XX DE C.acetobutylicum adhE, ctfA and ctfB genes XX KW acetoacetate decarboxylase; adhE gene; alcohol dehydrogenase; ctfA gene; KW ctfB gene. XX OS Clostridium acetobutylicum OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RX PUBMED; 8226639. RA Fischer R.J., Helms J., Durre P.; RT "Cloning, sequencing, and molecular analysis of the sol operon of RT Clostridium acetobutylicum, a chromosomal locus involved in RT solventogenesis"; RL J. Bacteriol. 175(21):6959-6969(1993). XX RN [2] RP 1-6352 RA Fischer R.J.; RT ; RL Submitted (05-MAY-1993) to the EMBL/GenBank/DDBJ databases. RL R.J. Fischer, Institut fuer Mikrobiologie, Grisebachstrasse 8, 3400 RL Goettingen, FRG XX CC Related sequences: M34078 & M93363 XX FH Key Location/Qualifiers FH FT source 1..6352 FT /organism="Clostridium acetobutylicum" FT /mol_type="genomic DNA" FT /db_xref="taxon:1488" FT CDS complement(<1..253) FT /transl_table=11 FT /note="ORF6" FT /db_xref="InterPro:IPR000601" FT /db_xref="UniProtKB/Swiss-Prot:P33747" FT /protein_id="CAA51341.1" FT /translation="MKKKLKSVLIWFLIFTFNLSLGSFREVFADNNDAVLATAITLSKN FT EDKLLVNQTDTLTAVVTPDNASNKGVTWSSSDTNTAAVD" FT RBS complement(261..266) FT /note="putative" FT /note="ORF6" FT -35_signal 507..512 FT /note="putative" FT /note="ORF5" FT -10_signal 530..535 FT /note="ORF5" FT /note="putative" FT mRNA 543..>1534 FT /note="ORF5" FT CDS 578..1534 FT /transl_table=11 FT /note="ORF5" FT /db_xref="GOA:P33746" FT /db_xref="HSSP:1ELR" FT /db_xref="InterPro:IPR019734" FT /db_xref="UniProtKB/Swiss-Prot:P33746" FT /protein_id="CAA51342.1" FT /translation="MNLLNLFTYVIPIAICIILPIFIIVTHFQIKSLNKAVTSFNKGDR FT SNALEILSKLVKSPIKNVKANAYITRERIYFYSRDFELSLRDLLQAIKLRPKTINDVYS FT FALSYHILGEPERALKYFLRAVELQPNVGISYENLAWFYYLTGKYDKAIENFEKAISMG FT STNSVYRSLGITYAKIGDYKKSEEYLKKALDAEPEKPSTHIYFSYLKRKTNDIKLAKEY FT ALKAIELNKNNFDGYKNLAEVNLAEDDYDGFYKNLEIFLEKINFVTNGEDFNDEVYDKV FT KDNEKFKELIAKTKVIKFKDLGIEIDDKKILNGKFLV" FT -35_signal 1918..1923 FT /note="putative start 1" FT -10_signal 1941..1946 FT /note="putative start 1" FT mRNA 1952..>4786 FT /gene="adhE" FT mRNA 1952..>5503 FT /gene="ctfA" FT mRNA 1952..>6170 FT /gene="ctfB" FT CDS 2059..2169 FT /transl_table=11 FT /note="ORFL" FT /db_xref="UniProtKB/TrEMBL:Q45814" FT /protein_id="CAA51343.1" FT /translation="MYCKPCFVLQFTISISKSAFKNNIYTKNNIDIIFKI" FT -35_signal 2078..2083 FT /note="putative start 2" FT -10_signal 2100..2105 FT /note="putative start 2" FT mRNA 2114..>4786 FT /gene="adhE" FT /note="alternative" FT mRNA 2114..>5503 FT /gene="ctfA" FT /note="alternative" FT mRNA 2114..>6170 FT /gene="ctfB" FT /note="alternative" FT RBS 2183..2188 FT /gene="adhE" FT /note="putative" FT CDS 2198..4786 FT /transl_table=11 FT /gene="adhE" FT /product="alcohol dehydrogenase E" FT /EC_number="1.1.1.1" FT /db_xref="GOA:P33744" FT /db_xref="InterPro:IPR012079" FT /db_xref="UniProtKB/Swiss-Prot:P33744" FT /protein_id="CAA51344.1" FT /translation="MKVTTVKELDEKLKVIKEAQKKFSCYSQEMVDEIFRNAAMAAIDA FT RIELAKAAVLETGMGLVEDKVIKNHFAGEYIYNKYKDEKTCGIIERNEPYGITKIAEPI FT GVVAAIIPVTNPTSTTIFKSLISLKTRNGIFFSPHPRAKKSTILAAKTILDAAVKSGAP FT ENIIGWIDEPSIELTQYLMQKADITLATGGPSLVKSAYSSGKPAIGVGPGNTPVIIDES FT AHIKMAVSSIILSKTYDNGVICASEQSVIVLKSIYNKVKDEFQERGAYIIKKNELDKVR FT EVIFKDGSVNPKIVGQSAYTIAAMAGIKVPKTTRILIGEVTSLGEEEPFAHEKLSPVLA FT MYEADNFDDALKKAVTLINLGGLGHTSGIYADEIKARDKIDRFSSAMKTVRTFVNIPTS FT QGASGDLYNFRIPPSFTLGCGFWGGNSVSENVGPKHLLNIKTVAERRENMLWFRVPHKV FT YFKFGCLQFALKDLKDLKKKRAFIVTDSDPYNLNYVDSIIKILEHLDIDFKVFNKVGRE FT ADLKTIKKATEEMSSFMPDTIIALGGTPEMSSAKLMWVLYEHPEVKFEDLAIKFMDIRK FT RIYTFPKLGKKAMLVAITTSAGSGSEVTPFALVTDNNTGNKYMLADYEMTPNMAIVDAE FT LMMKMPKGLTAYSGIDALVNSIEAYTSVYASEYTNGLALEAIRLIFKYLPEAYKNGRTN FT EKAREKMAHASTMAGMASANAFLGLCHSMAIKLSSEHNIPSGIANALLIEEVIKFNAVD FT NPVKQAPCPQYKYPNTIFRYARIADYIKLGGNTDEEKVDLLINKIHELKKALNIPTSIK FT DAGVLEENFYSSLDRISELALDDQCTGANPRFPLTSEIKEMYINCFKKQP" FT RBS 4834..4839 FT /gene="ctfA" FT /note="putative" FT CDS 4847..5503 FT /transl_table=11 FT /gene="ctfA" FT /product="acetoacetate decarboxylase" FT /EC_number="4.1.1.4" FT /note="subunit A" FT /db_xref="GOA:P33752" FT /db_xref="HSSP:1K6D" FT /db_xref="InterPro:IPR004163" FT /db_xref="UniProtKB/Swiss-Prot:P33752" FT /protein_id="CAA51345.1" FT /translation="MNSKIIRFENLRSFFKDGMTIMIGGFLNCGTPTKLIDFLVNLNIK FT NLTIISNDTCYPNTGIGKLISNNQVKKLIASYIGSNPDTGKKLFNNELEVELSPQGTLV FT ERIRAGGSGLGGVLTKTGLGTLIEKGKKKISINGTEYLLELPLTADVALIKGSIVDEAG FT NTFYKGTTKNFNPYMAMAAKTVIVEAENLVSCEKLEKEKAMTPGVLINYIVKEPA" FT RBS 5490..5495 FT /gene="ctfB" FT /note="putative" FT CDS 5505..6170 FT /transl_table=11 FT /gene="ctfB" FT /product="acetoacetate decarboxylase" FT /EC_number="4.1.1.4" FT /note="subunit B" FT /db_xref="GOA:P23673" FT /db_xref="HSSP:1O9L" FT /db_xref="InterPro:IPR004164" FT /db_xref="UniProtKB/Swiss-Prot:P23673" FT /protein_id="CAA51346.1" FT /translation="MINDKNLAKEIIAKRVARELKNGQLVNLGVGLPTMVADYIPKNFK FT ITFQSENGIVGMGASPKINEADKDVVNAGGDYTTVLPDGTFFDSSVSFSLIRGGHVDVT FT VLGALQVDEKGNIANWIVPGKMLSGMGGAMDLVNGAKKVIIAMRHTNKGQPKILKKCTL FT PLTAKSQANLIVTELGVIEVINDGLLLTEINKNTTIDEIRSLTAADLLISNELRPMAV" FT CDS complement(6236..>6352) FT /transl_table=11 FT /gene="adc" FT /db_xref="GOA:P23670" FT /db_xref="InterPro:IPR010451" FT /db_xref="PDB:3BH2" FT /db_xref="UniProtKB/Swiss-Prot:P23670" FT /protein_id="CAA51347.1" FT /translation="DHAMAPLNDLPVKEIVSSSHILADIILPRAEVIYDYLK" XX SQ Sequence 6352 BP; 2404 A; 946 C; 1037 G; 1965 T; 0 other; gatctaccgc tgctgtattt gtgtcacttg atgaccaggt tacaccttta tttgaagcat 60 tatcaggtgt aactacagcc gtaagcgtgt ctgtctgatt tactaaaagc ttatcttcat 120 ttttgcttaa agttattgcg gtggcaagta ctgcatcatt attatctgca aatacttctc 180 taaaggaacc tagacttaaa ttaaaagtaa aaattaaaaa ccatatcaga acacttttta 240 actttttttt cattttttat cccccttata gaatgtaata ataacattct tatctaattt 300 agttttctat ttttaactgg cagtaataaa gtttttgcat tgcaatggtc ggcgttaata 360 cgtgaacaat tgttatttag caaaaattat gttcaataca aatgactttg aaaacatttt 420 cttatatacc acattatata ttaatttgaa attaatgtaa acgtttcttg ttacaatttt 480 ctctcttaat ttttaattaa aaaaactcga taattttctt tgtatgtggt attatttgtg 540 tacataagta taaaaaaagg aaagagttga atttagcatg aatttattaa atctttttac 600 atatgtcata cctattgcga tatgtataat acttccaata tttataatag taacgcattt 660 tcaaatcaaa tctcttaaca aagctgtgac aagctttaat aagggtgata gaagcaatgc 720 cctagaaata ctatcaaaat tagtaaaaag tcctattaaa aatgttaaag caaatgccta 780 catcacaaga gaaagaatct acttctattc aagagacttt gaactttctt taagagattt 840 attacaggct attaaattaa gaccaaaaac aataaatgat gtttacagct ttgcacttag 900 ctatcacatt cttggagagc ctgaaagagc attaaaatat tttttaagag cagttgaact 960 ccagcctaat gtaggtatat cctacgagaa cttagcctgg ttttactact taacaggcaa 1020 atacgataag gcaatagaaa actttgaaaa agctatttcc atgggcagta caaactctgt 1080 ctatagaagt ttaggaatta cctatgccaa aataggagat tataaaaaat ccgaagaata 1140 tcttaagaaa gctttagacg cagaacctga aaaaccctca actcatatat acttttcgta 1200 tttaaaaaga aagaccaatg atattaaact agcaaaggaa tatgctctaa aagcaattga 1260 gcttaataaa aataattttg atggttataa aaatcttgct gaagtaaacc ttgctgagga 1320 tgattatgac ggcttctata aaaatcttga aatattttta gaaaaaataa attttgtaac 1380 taatggagaa gactttaatg atgaagttta tgataaagtt aaagataatg aaaagtttaa 1440 ggagcttata gctaaaacca aagtaattaa atttaaagat ttaggcatag aaatcgatga 1500 taaaaaaata cttaacggaa aatttttagt ataaaagaag ctatatctta attcaaaatt 1560 aagatatagc ttcttttatg tagtattatt tcagaagtct acaaattaag tttatattta 1620 gaccctgggg tgtaactata gtatttaata ttggtactat taattagggt tatatatact 1680 agaacttatc atggtaaaca taaatataaa ctcaattcta tttatgctcc tataaaattt 1740 tataatatag gaaaactgct aaatgtaaat tatacgttta catttagcag tttattttaa 1800 accttcatat ttttctaaat atactgataa ttcctaaata tatattatta cgccaaaata 1860 ttagatacca ttttgtaaaa gttgctattt acatttaaat acacagctgt tatatttttg 1920 acctatgctt tttattgaac tataataaaa gcatagtaat tagtaattaa taatttatat 1980 tattaattat cataaaattt atgatccttt aaagtaacat ttttatttac ttcataaatt 2040 gatgttattt ttttcttgat gtattgtaaa ccttgttttg ttttgcagtt tacaatatct 2100 atctccaaat ctgctttcaa gaataatatc tatactaaaa acaatataga cattattttt 2160 aaaatataat aaatatttta gaaagaagtg tatatttatg aaagtcacaa cagtaaagga 2220 attagatgaa aaactcaagg taattaaaga agctcaaaaa aaattctctt gttactcgca 2280 agaaatggtt gatgaaatct ttagaaatgc agcaatggca gcaatcgacg caaggataga 2340 gctagcaaaa gcagctgttt tggaaaccgg tatgggctta gttgaagaca aggttataaa 2400 aaatcatttt gcaggcgaat acatctataa caaatataag gatgaaaaaa cctgcggtat 2460 aattgaacga aatgaaccct acggaattac aaaaatagca gaacctatag gagttgtagc 2520 tgctataatc cctgtaacaa accccacatc aacaacaata tttaaatcct taatatccct 2580 taaaactaga aatggaattt tcttttcgcc tcacccaagg gcaaaaaaat ccacaatact 2640 agcagctaaa acaatacttg atgcagccgt taagagtggt gccccggaaa atataatagg 2700 ttggatagat gaaccttcaa ttgaactaac tcaatattta atgcaaaaag cagatataac 2760 ccttgcaact ggtggtccct cactagttaa atctgcttat tcttccggaa aaccagcaat 2820 aggtgttggt ccgggtaaca ccccagtaat aattgatgaa tctgctcata taaaaatggc 2880 agtaagttca attatattat ccaaaaccta tgataatggt gttatatgtg cttctgaaca 2940 atctgtaata gtcttaaaat ccatatataa caaggtaaaa gatgagttcc aagaaagagg 3000 agcttatata ataaagaaaa acgaattgga taaagtccgt gaagtgattt ttaaagatgg 3060 atccgtaaac cctaaaatag tcggacagtc agcttatact atagcagcta tggctggcat 3120 aaaagtacct aaaaccacaa gaatattaat aggagaagtt acctccttag gtgaagaaga 3180 accttttgcc cacgaaaaac tatctcctgt tttggctatg tatgaggctg acaattttga 3240 tgatgcttta aaaaaagcag taactctaat aaacttagga ggcctcggcc atacctcagg 3300 aatatatgca gatgaaataa aagcacgaga taaaatagat agatttagta gtgccatgaa 3360 aaccgtaaga acctttgtaa atatcccaac ctcacaaggt gcaagtggag atctatataa 3420 ttttagaata ccaccttctt tcacgcttgg ctgcggattt tggggaggaa attctgtttc 3480 cgagaatgtt ggtccaaaac atcttttgaa tattaaaacc gtagctgaaa ggagagaaaa 3540 catgctttgg tttagagttc cacataaagt atattttaag ttcggttgtc ttcaatttgc 3600 tttaaaagat ttaaaagatc taaagaaaaa aagagccttt atagttactg atagtgaccc 3660 ctataattta aactatgttg attcaataat aaaaatactt gagcacctag atattgattt 3720 taaagtattt aataaggttg gaagagaagc tgatcttaaa accataaaaa aagcaactga 3780 agaaatgtcc tcctttatgc cagacactat aatagcttta ggtggtaccc ctgaaatgag 3840 ctctgcaaag ctaatgtggg tactatatga acatccagaa gtaaaatttg aagatcttgc 3900 aataaaattt atggacataa gaaagagaat atatactttc ccaaaactcg gtaaaaaggc 3960 tatgttagtt gcaattacaa cttctgctgg ttccggttct gaggttactc cttttgcttt 4020 agtaactgac aataacactg gaaataagta catgttagca gattatgaaa tgacaccaaa 4080 tatggcaatt gtagatgcag aacttatgat gaaaatgcca aagggattaa ccgcttattc 4140 aggtatagat gcactagtaa atagtataga agcatacaca tccgtatatg cttcagaata 4200 cacaaacgga ctagcactag aggcaatacg attaatattt aaatatttgc ctgaggctta 4260 caaaaacgga agaaccaatg aaaaagcaag agagaaaatg gctcacgctt caactatggc 4320 aggtatggca tccgctaatg catttctagg tctatgtcat tccatggcaa taaaattaag 4380 ttcagaacac aatattccta gtggcattgc caatgcatta ctaatagaag aagtaataaa 4440 atttaacgca gttgataatc ctgtaaaaca agccccttgc ccacaatata agtatccaaa 4500 caccatattt agatatgctc gaattgcaga ttatataaag cttggaggaa atactgatga 4560 ggaaaaggta gatctcttaa ttaacaaaat acatgaacta aaaaaagctt taaatatacc 4620 aacttcaata aaggatgcag gtgttttgga ggaaaacttc tattcctccc ttgatagaat 4680 atctgaactt gcactagatg atcaatgcac aggcgctaat cctagatttc ctcttacaag 4740 tgagataaaa gaaatgtata taaattgttt taaaaaacaa ccttaaacct tcatatttca 4800 actacttttt ataattttaa taaagaattt aaaaggaggg attaaaatga actctaaaat 4860 aattagattt gaaaatttaa ggtcattctt taaagatggg atgacaatta tgattggagg 4920 ttttttaaac tgtggcactc caaccaaatt aattgatttt ttagttaatt taaatataaa 4980 gaatttaacg attataagta atgatacatg ttatcctaat acaggtattg gtaagttaat 5040 atcaaataat caagtaaaaa agcttattgc ttcatatata ggcagcaacc cagatactgg 5100 caaaaaactt tttaataatg aacttgaagt agagctctct ccccaaggaa ctctagtgga 5160 aagaatacgt gcaggcggat ctggcttagg tggtgtacta actaaaacag gtttaggaac 5220 tttgattgaa aaaggaaaga aaaaaatatc tataaatgga acggaatatt tgttagagct 5280 acctcttaca gccgatgtag cattaattaa aggtagtatt gtagatgagg ccggaaacac 5340 cttctataaa ggtactacta aaaactttaa tccctatatg gcaatggcag ctaaaaccgt 5400 aatagttgaa gctgaaaatt tagttagctg tgaaaaacta gaaaaggaaa aagcaatgac 5460 ccccggagtt cttataaatt atatagtaaa ggagcctgca taaaatgatt aatgataaaa 5520 acctagcgaa agaaataata gccaaaagag ttgcaagaga attaaaaaat ggtcaacttg 5580 taaacttagg tgtaggtctt cctaccatgg ttgcagatta tataccaaaa aatttcaaaa 5640 ttactttcca atcagaaaac ggaatagttg gaatgggcgc tagtcctaaa ataaatgagg 5700 cagataaaga tgtagtaaat gcaggaggag actatacaac agtacttcct gacggcacat 5760 ttttcgatag ctcagtttcg ttttcactaa tccgtggtgg tcacgtagat gttactgttt 5820 taggggctct ccaggtagat gaaaagggta atatagccaa ttggattgtt cctggaaaaa 5880 tgctctctgg tatgggtgga gctatggatt tagtaaatgg agctaagaaa gtaataattg 5940 caatgagaca tacaaataaa ggtcaaccta aaattttaaa aaaatgtaca cttcccctca 6000 cggcaaagtc tcaagcaaat ctaattgtaa cagaacttgg agtaattgag gttattaatg 6060 atggtttact tctcactgaa attaataaaa acacaaccat tgatgaaata aggtctttaa 6120 ctgctgcaga tttactcata tccaatgaac ttagacccat ggctgtttag aaagaaatac 6180 tatgaaacaa tattaaaaaa ataagagtta ccatttaagg taactcttat ttttattact 6240 taagataatc atatataact tcagctctag gcaatattat atctgcaaga atgtgagagc 6300 tagaaacaat ctcttttact ggcaaatcat taagtggcgc catagcgtga tc 6352 // |
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