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EBI DbfetchID X70378; SV 1; linear; genomic DNA; STD; PLN; 5645 BP. XX AC X70378; XX DT 23-FEB-1993 (Rel. 35, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 10) XX DE D.caryophyllus fht gene for narigenin 3-dioxygenase XX KW 2-oxoglutarate-dependent dioxygenase; KW dihydrokaempferol, 2-oxoglutarate-dependent dioxygenase; KW flavanone 3-hydroxylase; flavonoid gene; hydroxylase; KW naringenin,2-oxoglutarate 3-dioxygenase. XX OS Dianthus caryophyllus (clove pink) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; OC Caryophyllales; Caryophyllaceae; Dianthus. XX RN [1] RA Dedio J., Saedler H., Forkmann G.; RT "Cloning and nucleotide sequence determination of the flavanone RT 3-hydroxylase gene from Dianthus caryophyllus (carnation)"; RL Unpublished. XX RN [2] RP 1-5645 RA Dedio J.; RT ; RL Submitted (10-FEB-1993) to the EMBL/GenBank/DDBJ databases. RL J. Dedio, Max-Planck-Inst fuer Zuechtungsforschung, Carl-von-Linne-Weg 10, RL 5000 Koeln 30, FRG XX FH Key Location/Qualifiers FH FT source 1..5645 FT /organism="Dianthus caryophyllus" FT /mol_type="genomic DNA" FT /clone_lib="MboI partial, EMBL4" FT /db_xref="taxon:3570" FT CDS join(604..966,3070..3498,4172..4477) FT /gene="fht" FT /standard_name="naringenin,2-oxoglutarate 3-dioxygenase" FT /standard_name="flavanone 3-hydroxylase" FT /standard_name="dihydrokaempferol, 2-oxoglutarate-dependent FT dioxygenase" FT /product="naringenin 3-dioxygenase" FT /EC_number="1.14.11.9" FT /db_xref="GOA:Q05964" FT /db_xref="InterPro:IPR005123" FT /db_xref="UniProtKB/Swiss-Prot:Q05964" FT /protein_id="CAA49839.1" FT /translation="MVAEKPKTLTSLEGDDKLNSNFVRDEDERPKVAYNEFSNDIPVIS FT LAGIDGEKRGEICRKIVEACEDWGIFQVVDHGVGDDLIADMTRLAREFFALPAEEKLRF FT DMSGGKKGGFIVSSHLQGEVVQDWREIVTYFSYPTNSRDYTRWPDKPEGWIKVTEEYSN FT KLMTLACTLLGVLSEAMGLELEALTKACVDMDQKIVVNYYPKCPQPDLTLGLKRHTDPG FT TITLLLQDQVGGLQATRDGGKTWITVQPVPGAFVVNLGDHGHFLSNGRFKNADHQAVVN FT SECSRLSIATFQNPSPDATVYPLAIREGENSIMEEPITFADLYRRKMAKDLEIARHKRL FT AKEEMPFKELDEAKFESKSIDQILA" FT mRNA join(459..966,3070..3498,4172..4749) FT /gene="fht" FT /experiment="experimental evidence, no additional details FT recorded" FT exon 459..966 FT /gene="fht" FT /number=1 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 3070..3498 FT /gene="fht" FT /number=2 FT /experiment="experimental evidence, no additional details FT recorded" FT exon 4172..4749 FT /gene="fht" FT /number=3 FT /experiment="experimental evidence, no additional details FT recorded" FT intron 967..3069 FT /gene="fht" FT /number=1 FT intron 3499..4171 FT /gene="fht" FT /number=2 FT CAAT_signal 378..386 FT /gene="fht" FT TATA_signal 429..439 FT /gene="fht" FT polyA_signal 4658..4663 FT /gene="fht" FT polyA_site 4750 FT /gene="fht" FT /experiment="experimental evidence, no additional details FT recorded" XX SQ Sequence 5645 BP; 1875 A; 831 C; 1016 G; 1923 T; 0 other; gaattcagta attctcctgt gcaggggcgg acccacctaa tagcaagggg tggcagccgc 60 taccccatac acaaaaaagt cggtttaaaa atggatttcc gctacaccaa atatcaatga 120 aaaatataat gttacccagt ataatgaaca ataaatatac ttttgctact gtaaacacta 180 atttttgggt ctgcccgtgc tcctgtgaac attaaaattt atgatttgca tagaggaaac 240 gacaaataca actaccagtg gttatagaga agacaagtac gtgagaaaat aagtgacaaa 300 gacgttggtc catgcatgta cgcatggcca agttacgacc gaatttaatt tcgcacgaga 360 tttatggttg ttacagcggc caatcttaat tttttttttt tttgaatttt cctcctccac 420 ctatcttata tatataatat gttgtcactt actatatctt aaatattcac aacattataa 480 cataagcttc aaaataacat tattccgata tttacgtaat ataatacgta tcatattagg 540 gtacattcat tttatcaact acgactgcat attgttagac agtctcatat atacgcataa 600 aaaatggtcg ctgaaaaacc caaaacgctc acttcactag aaggggacga taaattgaac 660 tcgaattttg ttagggacga ggatgaacgt ccgaaagtgg cgtataatga gtttagcaat 720 gatattccgg tgatatctct tgctggtata gatggtgaaa aaaggggtga aatatgtcgg 780 aagattgttg aggcgtgtga agattggggg atttttcaag tggttgatca cggtgttggt 840 gacgatctta ttgctgatat gactcggttg gctcgtgaat ttttcgctct cccggcagaa 900 gagaagctcc gatttgatat gtctggtggt aaaaagggcg gttttatcgt gtcgagtcat 960 cttcaggttc atattgtttc ttcaattgtt tataaacgtt ttaacaaagt aatttatttg 1020 taataatctt tccatttaca tttaattgtc aaatttactt tttaaattga acttttacct 1080 attttaagtt tggataagtc ctgaaatacg aaaattttgg acatgaaaat aacatactct 1140 ttaaaatcat tattaaaaaa aatgttctaa gcgatcataa ttttatcaaa acaaaattca 1200 tactccttat gtactttaga taaaaatgtg gtaaaagtat tatctcgaat accatgtaat 1260 tcaggttcag tgatttgttt acagtttgac ttttttgtga gagggattta aggaaagtat 1320 aaacaaatga gtgagacaga gtatattgat tttgaatggt agttatattg gagtattatt 1380 ctacgttatc atgtgaaatg tcaagctacg tacatttttg tttaattttc acaagaatgg 1440 gtgtttttgt ggaatgtcta aaagagaaag ttgtgtatgg tttgcaaaaa actgtttaat 1500 tttcacatga atgggtgttt ttgtgtatgg tttgcaaaaa actgtatggt ttgcaaaaaa 1560 ctgtttggta tgcaaaaaac tgtttaattt tcacatgaat gggtgttttt gtgtatggtt 1620 tgcaaaaaac tgtttggttg gtatgctgat agtaactttc tacaaaaaat aaaaaattct 1680 acatgtcggt aaatgaattc ctccataata aatagatgaa tgttcatgag aagtttcaat 1740 ttcaatggaa taggcaaaca aacaacttga acatctttca gttgatggaa attttaaatt 1800 ctcatgaatt taaaatcaaa cgatccctag gttttgacat tttgttaaat caataataat 1860 ttcaaaataa atattctata aacaaatact ctgtaattga tttgagaaat ttaaataata 1920 atggatcaga tatgtacaaa aatattactc tgcatttaat ttgatttggt gtatgtcatg 1980 ttttggacat cattagttag ttacttgtgt tattgatatg agttgtttat tttattttat 2040 tttgttttcc caaccgatat ccgaacccat ttgggtcgac taattcggtt ttggagcgtg 2100 tttcggggtt aaggttttaa actcctccca acgggcttgc tggggatcga acctcagttc 2160 tccctaccaa gtctagcccc tggcaccact agaccaagac ctcgttggta ttgatacgag 2220 ttgtttatga aagtggaatt taacatgttt agagggatct tatgcaccat ttagaaatcc 2280 gcaaaattta gtaggattat tttacaagaa taattcatac tattgttgat tttctctaaa 2340 taatccgaat tattgtttat ctcaaaataa acccaacttt gatagttatc ttctcaaaat 2400 aatctatact gaaagttttt tttctcaaaa taaacccaaa taattccata tagtttattt 2460 gagaagacaa ctatcaaagt tgagtttatt tttgagataa acaatagttc ggattatttt 2520 gagaagaata ccaatagtat ggattattct tgtaaaataa tccaatttat tatacaaggg 2580 atttacgcat atggtggagt tattatggac tagaatgtaa ccatttaatg tataatatgt 2640 ctactttaac ttttaaattg accactttaa tcttaaaaag tggtcacatt atattaaata 2700 ttaaattatc actttgaatg ttaaaattgt cacatttagt cactaataat ccgtcatatc 2760 tataaatccg tcgtacaata gaaatacttt ttgatacgaa agtaattatg attatttcct 2820 tcttaaactt attatgcttc attttgaagt tgtaggattt aacgctacgc atatataaga 2880 aaatctagat caacggaaca atatttaatg agtcaaaaat cggttaaagt ataaaaacta 2940 atctttggcc tccaaccatc agtttaagct tttttactgg aagcctaaga ttgattttta 3000 tactctaaca aagtcgacat catatgaccc gaaactaacc ccgagttgaa tcgaaatccg 3060 aatcaacagg gagaagtagt gcaggactgg agggaaatcg tgacgtattt ctcatacccg 3120 acgaactcaa gggactacac aagatggcca gacaaaccag agggttggat aaaggtcaca 3180 gaggaataca gcaacaagtt aatgacctta gcatgtacac ttttaggtgt actttctgaa 3240 gccatgggtt tagaattaga ggcacttact aaagcttgtg ttgatatgga ccaaaagatt 3300 gtggttaatt actaccctaa gtgccctcaa cctgacctta ctttagggct caagaggcac 3360 accgaccccg ggactataac cctcctcctt caggaccaag tcggcggtct tcaggccact 3420 cgtgacggtg gtaaaacttg gattaccgtg cagccggttc ccggtgcctt cgttgttaac 3480 cttggtgatc atggtcatgt gagttcacta cttcctttag ttttatggtt tgtttttttc 3540 gtttctgtcg agtatgtctg tgatgaaacc gtcttactta ggatattaag aaatagtcac 3600 gtagctaaga aattaatgtg tcgtttttat tctctttttt ttttttttga tgaaaaactt 3660 tacttgactt cctttttttt tttttttttt aaacttaaga atgtttgaac gtaaatatac 3720 gagaaactat taaaaagaat tagagtaaaa tgaaagattg tcttaccgtc aaatgattga 3780 aaacgtattc aaagtttggt cttgaaaact gttgtatgaa gtcaaagtgg aagttttcaa 3840 agaagtcggg agtatatgtc gttttggtcg agtatggttg tggtgaaatt gtcttaccgt 3900 aatatatcaa gatatattgt cacccaaaaa aattactccg tatatcaaga atatatgcga 3960 aaaatttaac gtgtcgtttt aattgtactc cctatatttt gatgaaagct ttcagttttt 4020 ttgtgcaaat tgatatttat ggtgaaaatt gcagttaaag tgtcgtcttg aaaattgtac 4080 aaagtcaaag cggaatttta aaaaagtaga ggcagtatat tttagctatt ggaatattct 4140 aattaaacat gtgacgtgat tttgggtgaa gtttttgagc aatgggagat tcaaaaatgc 4200 ggatcaccaa gcggtggtga actcggaatg cagccgccta tcaatcgcaa cgtttcaaaa 4260 cccgtcacca gacgcgacgg tctacccatt ggcaataaga gaaggtgaga attcaattat 4320 ggaagaacca ataactttcg ccgatttgta tcggcgaaaa atggccaaag accttgagat 4380 cgcccgtcat aagaggcttg ctaaagagga aatgcctttt aaagagttgg acgaggccaa 4440 gtttgagtcc aaatctattg accaaatact tgcttagatg ggcttggttt ggtttcatta 4500 tattaaattt attattatta ttatttattg catttgatat gatatgattg gaaataaaag 4560 agagattgtt tgtgataatt tgtgtgatta ttatatcact aagttatggc tttaatttgt 4620 ggtatgttgg gaattatata tttagttttg tgtgaagaat atatgattta aagttaaaaa 4680 aaaaaatgat ttgttatatg atttacttgt aaggttataa ggttatattt attgttcgag 4740 tttgcgtata tattactcct acaatacaat ttaaaaagag tctaattatt taaaaattac 4800 tccatatgat aaatcgtcgc actataagat cgtctaatgt aataatattg gttcattaaa 4860 ttgttaagac ttacttttat gtttggtttt tctcttctgg accattctat gctacataaa 4920 ggtttcatag agaatttgct aaaagaaaaa ctagtaattc tttcataaga ctgatttata 4980 ccataagatg agcttattac aacgtacaag gatggttgaa ttattgaatt tttttaagta 5040 taattgttac tccgtaaatc gttaaacaga ttgaacatat ctacggagta agttataata 5100 ctccagtaag atcatttcat tcgataattg gtaaaacaat taactagccg aattaattac 5160 gaagaatagt acccacaaaa ctttaaactt ttaaaggaac aaaagaaaag tagcgacaaa 5220 tcaaacctag accataaata aacgtctaaa taatccaact aacatttcca ccaaaagttc 5280 tctactatgt gacgacatat atacgacgat atcaacattg ctcgagaagt ctagtgcaat 5340 ttattacaaa ccaaacacgt tacacgtatt acgaatgtaa cgtagttatg ggcttgtaat 5400 gcgatcgaaa aataaacaga aatgaaaaag atagaaaaat atagttgttc gatcgttcag 5460 attgataggc tactttaata atagagttgt gatgattggt gaaatatata aactcctaga 5520 ttcgagtaac tcgatgtgac aaactcgttc attcaattca tgatatatat aggtctaagg 5580 cttggagaaa atggaacggt aaaattacta gactaccatt ccactaattt gacatggata 5640 agctt 5645 // |
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