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EBI DbfetchID X69020; SV 1; linear; genomic DNA; STD; PRO; 2170 BP. XX AC X69020; XX DT 03-FEB-1993 (Rel. 34, Created) DT 12-JUN-2006 (Rel. 88, Last updated, Version 2) XX DE Pseudomonas sp. (strain V22) gene for cephalosporin acylase XX KW acylase; cephalosporin acylase. XX OS Pseudomonas sp. OC Bacteria; Proteobacteria. XX RN [1] RP 1-2170 RX DOI; 10.1016/0167-4781(92)90155-S RX PUBMED; 1358202. RA Ishiye M., Niwa M.; RT "Nucleotide sequence and expression in Escherichia coli of the RT cephalosporin acylase gene of a Pseudomonas strain"; RL Biochim. Biophys. Acta 1132(3):233-239(1992). XX RN [2] RP 1-2170 RA Niwa M.; RT ; RL Submitted (06-JAN-1993) to the EMBL/GenBank/DDBJ databases. RL M. Niwa, Product Development Laboratories, Fujiswawa Pharmaceutical Co, 1-6 RL 2 Chome Kashima, Yodogawa-ku, Osaka 532, JAPAN XX FH Key Location/Qualifiers FH FT source 1..2170 FT /organism="Pseudomonas sp." FT /strain="V22" FT /mol_type="genomic DNA" FT /db_xref="taxon:306" FT RBS 341..344 FT RBS 350..353 FT CDS 359..2035 FT /transl_table=11 FT /product="cephalosporin acylase" FT /db_xref="GOA:Q05053" FT /db_xref="InterPro:IPR000101" FT /db_xref="UniProtKB/Swiss-Prot:Q05053" FT /protein_id="CAA48785.1" FT /translation="MNAPVPVPRVADFTCEKKPATGSRGMVVTNHPLASAAGAQILLAG FT GNAIDAAVASLFALTVAEPMMVGILGGGLSHIRLADGRHVVIDNLSTAPGKATADMYEC FT LSDEIGKQRDTRDRENVVGAKAVAVPGALKGWCEALARFGTLPLAEVLQPAIGLAERGF FT VVTPYLSNCITDNAADLARDPGLAAMLLPGGQPLQPGMRLIQSDYAASLKLIAAEGPEA FT LYGGKLGRALTDYMAANGGLIDQADLSNYRIELREPIRGSYRGYEIIGPPPPSSSGVHI FT AQMLNILEGYDIGALGFGSTDAVHLLAEALKIAFADRAVATADPAFVKVPVARLIDKAY FT ADERRALIAMEQAKSWTAGLSGGESADTTHVTVADAMGNVVSATQTINGLFGACVQTPG FT TGMIANNYMYNFDPHPGRALSIAPGKRVFTSMAPMMAVKEGRLAFALGLPGALRIFPSA FT LQAIVNLIDHRMSLQEAVEAPRVWTEGGVLELEEAIPESVAQALIARGHKVVRSPRVAG FT GMNAIAFNPDGTLTGAACWRADGTPVAISGGLARAGARFTI" XX SQ Sequence 2170 BP; 339 A; 774 C; 721 G; 336 T; 0 other; gtcgacgggc cctgcactga cctcaccgag atcgaggatt ccgatttccc gatgtggtgc 60 cgcggcatgt cgccgatcac cacacggctc tacaatctcg gcggcacgct caacctgccg 120 atctcctgcg gcaatgtccc ggtgaaggcc ggcgacgtca tcctcgccga cgaatccggc 180 gtgctggttc tgccgcgcgc cgaggccgag gcgatcgcgg acgccgccat cgcccggcag 240 gagcgcggcg agcgcagcca ggctcgggtg aaggcgggcg agaagctcgg cgacatctcc 300 ggcgcgacca agatggtgct cgacgccatc gcggcggcga aaggctgaag aggctgccat 360 gaacgctccc gtcccggtcc cgcgcgtcgc cgacttcacc tgcgagaaga agccggcgac 420 cggctcgcgc ggcatggtcg ttaccaacca tccgctcgct tcggcggcgg gtgcgcagat 480 cctgctcgcc ggcggcaatg ccatcgacgc ggccgtcgcc tcgctcttcg cgctgacggt 540 ggccgagccg atgatggtcg gcattctcgg cggtggcctg agccatatcc ggctcgccga 600 cgggcgccat gtcgtgatcg acaacctctc gaccgcgccg ggcaaggcga cggccgacat 660 gtatgagtgc ctgtccgacg agatcggcaa gcagcgcgac acgcgcgacc gcgagaacgt 720 ggtcggagcc aaggcggtcg cggttcccgg tgcgctcaag ggctggtgcg aggcgctcgc 780 ccgcttcggc acgttgccgc tggccgaggt gctgcaacct gcgatcggac tggccgagcg 840 cggcttcgtg gtgacgccct atctctcgaa ctgcatcacc gataatgcgg ccgacctcgc 900 ccgcgatccc ggccttgcgg cgatgctgct gccgggcggg cagccgctcc agccgggcat 960 gcggctcatc cagtccgact atgccgcgag cttgaagctg atcgcggcgg aggggccaga 1020 ggcgctctat ggcgggaaac tcggacgggc gctgaccgat tacatggcgg cgaatggcgg 1080 cctgatcgat caggccgacc tctcgaatta ccgcatcgaa ttgcgcgagc cgatccgggg 1140 ctcctatcgc ggctacgaga tcatcggccc gccgccgccc tcgtcatcgg gcgtgcatat 1200 cgcgcagatg ctcaacatcc tcgaaggcta cgatatcggc gcgctcggct tcggctcgac 1260 ggacgccgtg catctgctcg ccgaggcgct gaagatcgcc ttcgccgacc gcgccgtggc 1320 gaccgccgat ccggccttcg tcaaggttcc ggtcgcgagg ctgatcgaca aggcctatgc 1380 cgacgaacgc cgcgccctga tcgcaatgga gcaggcaaag agctggacgg ccgggctgtc 1440 aggcggcgaa tccgcggata cgacccatgt caccgtcgcc gacgccatgg gcaatgtcgt 1500 cagcgcgacg cagacgatca acgggctgtt cggtgcctgt gtgcagactc ccggcacggg 1560 gatgatcgcc aacaactaca tgtacaactt cgatccgcat cccggccggg cgctctcgat 1620 cgcgccgggc aagcgggtct tcacctcgat ggcgccgatg atggcggtga aggaaggccg 1680 gctcgccttc gcgctcgggc tgcccggcgc cttgcgcatc ttcccctcgg cgctgcaggc 1740 gatcgtcaac ctgatcgacc accggatgag cctgcaggag gcggtcgagg cgccgcgcgt 1800 ctggaccgaa ggcggcgttc tcgaactcga ggaagccatc cccgagtccg tggcgcaggc 1860 gctgatcgcg cgcggccata aggtcgtgcg ctcgccgcgc gtcgccggcg gcatgaacgc 1920 catcgccttc aacccggacg gcaccctgac cggcgccgcc tgctggcgcg ccgacggcac 1980 gcccgtcgcc atctccggcg ggctcgcccg cgccggtgcc cgcttcacca tctgacccga 2040 catcttcacc gatcctgcga ctggaccatc ccatgcccga gaccatctgc ctgctcgaca 2100 tgaccacgcc cgagcgggcc gaccgcctgc gcgaattcct gcctgacgct tcgtgctgac 2160 ccatggtacc 2170 // |
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