EBI Dbfetch

ID   X67958; SV 1; linear; mRNA; STD; PLN; 1978 BP.
XX
AC   X67958;
XX
DT   19-AUG-1992 (Rel. 33, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 9)
XX
DE   A.officinalis mRNA for asparagine synthetase
XX
KW   asparagine synthetase.
XX
OS   Asparagus officinalis (garden asparagus)
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; Liliopsida; Asparagales; Asparagaceae;
OC   Asparagus.
XX
RN   [1]
RP   1-1978
RA   Davies K.M.;
RT   ;
RL   Submitted (26-JUN-1992) to the EMBL/GenBank/DDBJ databases.
RL   K.M. Davies, Levin Horticultural  Research Centre, MAF Technology, Private
RL   Bag, Levin, NEW ZEALAND
XX
RN   [2]
RX   DOI; 10.1104/pp.102.4.1337
RX   PUBMED; 7904077.
RA   Davies K.M., King G.A.;
RT   "Isolation and characterization of a cDNA clone for a harvest-induced
RT   asparagine synthetase from Asparagus officinalis L";
RL   Plant Physiol. 102(4):1337-1340(1993).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1978
FT                   /organism="Asparagus officinalis"
FT                   /cultivar="Limbras10"
FT                   /mol_type="mRNA"
FT                   /dev_stage="mature plant"
FT                   /clone_lib="lambda-ZAPII"
FT                   /clone="pTIP27"
FT                   /tissue_type="spear"
FT                   /db_xref="taxon:4686"
FT   CDS             56..1828
FT                   /product="asparagine synthase (glutamine-hydrolysing)"
FT                   /EC_number="6.3.5.4"
FT                   /db_xref="GOA:P31752"
FT                   /db_xref="HSSP:1CT9"
FT                   /db_xref="InterPro:IPR014729"
FT                   /db_xref="UniProtKB/Swiss-Prot:P31752"
FT                   /protein_id="CAA48141.1"
FT                   /translation="MCGILAVLGCSDDSQAKRVRVLELSRRLKHRGPDWSGLCQHGDCF
FT                   LSHQRLAIIDPASGDQPLYNEDKSIVVTVNGEIYNHEELRRRLPDHKYRTGSDCEVIAH
FT                   LYEEHGEDFVDMLDGMFSFVLLDTRNNCFVAARDAVGITPLYIGWGLDGSVWLSSEMKG
FT                   LNDDCEHFEVFPPGNLYSSRSGSFRRWYNPQWYNETIPSAPYDPLVLRKAFEDAVIKRL
FT                   MTDVPFGVLLSGGLDSSLVAAVTARHLAGSKAAEQWGTQLHSFCVGLEGSPDLKAAKEV
FT                   AEYLGTVHHEFHFTVQDGIDAIEDVIFHIETYDVTTIRASTPMFLMARKIKSLGVKMVI
FT                   SGEGSDEIFGGYLYFHKAPNKEEFHHETCRKIKALHQYDCLRANKATSAWGLEARVPFL
FT                   DKEFMDVAMSIDPESKMIKPDLGRIEKWVLRKAFDDEENPYLPKHILYRQKEQFSDGVG
FT                   YSWIDGLKAHAAKHVTDRMMLNAARIYPHNTPTTKEAYYYRMIFERFFPQNSARFTVPG
FT                   GPSIACSTAKAIEWDARWSNNLDPSGRAALGVHDSAYDPPLPSSISAGKGAAMITNKKP
FT                   RIVDVATPGVVIST"
FT   polyA_site      1925
XX
SQ   Sequence 1978 BP; 566 A; 396 C; 513 G; 503 T; 0 other;
     tctgcttgca ccttttgaga gagagggaga gagagagaga gagagagaga ggatcatgtg        60
     tgggatactt gcagtgctcg gttgctccga tgactctcag gcgaagaggg ttcgagttct       120
     cgagctctct cgcaggttga agcacagggg cccagattgg agcgggcttt gccaacatgg       180
     agattgtttc ttgtctcatc agagattggc gatcattgat cccgcctctg gtgatcaacc       240
     cctgtacaac gaggacaagt ccatcgttgt cacggtaaac ggagagattt acaaccacga       300
     agagctaagg cgacgcctgc ctgatcataa atacagaact ggaagcgact gtgaagtcat       360
     cgctcatctg tatgaggaac acggagaaga tttcgtcgat atgttggatg gaatgttctc       420
     cttcgttcta ttggacaccc gaaacaattg cttcgttgcg gcaagggatg cagtgggaat       480
     aacccccctc tacattggct ggggattaga cggctctgtt tggctctcgt cggaaatgaa       540
     aggattaaac gatgactgcg aacattttga agtatttcca cctggaaacc tgtactcaag       600
     cagatcaggc agcttcagaa gatggtataa tcctcagtgg tacaatgaga ctatcccttc       660
     ggccccctat gatcctcttg ttctgaggaa agcttttgag gatgctgtta taaagaggct       720
     gatgactgat gtgccatttg gggttctgtt atctggtggc ctcgattcct cgttggtcgc       780
     cgctgttact gctcggcatc ttgcaggaag taaagctgca gagcaatggg gaactcagct       840
     ccattctttc tgtgttggct tagagggatc accagatctc aaggctgcaa aagaggttgc       900
     agagtatctg ggtactgtcc accatgagtt tcacttcaca gttcaggatg gaattgatgc       960
     cattgaggat gtaatcttcc acattgaaac gtacgatgtg acaacaatca gggcaagcac      1020
     tccaatgttc ctcatggcca gaaaaatcaa gtccttagga gtaaaaatgg tgatctcagg      1080
     cgaaggctcg gatgaaatct ttggcgggta cttgtatttt cacaaagcac ctaacaaaga      1140
     agaattccat cacgaaacat gtcgaaagat caaagctctg catcagtatg actgcctcag      1200
     agccaacaaa gcaacatcag catgggggct ggaagctcga gtgccatttt tagacaagga      1260
     gttcatggat gttgctatga gtatagatcc tgaatcgaaa atgattaagc ctgatctcgg      1320
     gaggatcgag aagtgggtac tgaggaaagc ttttgatgat gaagagaatc cctatcttcc      1380
     aaagcatatt ctctataggc aaaaggagca gttcagtgat ggtgttggat atagttggat      1440
     tgatgggctg aaggctcatg ctgcaaaaca tgtaactgat agaatgatgc tgaatgcagc      1500
     acgtatttac ccccacaaca caccaaccac aaaagaggct tattactaca gaatgatctt      1560
     tgaaaggttc ttccctcaga actcggcgag atttactgtc cctggaggtc caagcattgc      1620
     ttgcagcacg gcgaaggcta tcgaatggga cgctcgctgg tcgaacaatt tggatccgtc      1680
     ggggagagca gctctcggcg tccatgactc tgcctacgat cctcctcttc cttcttcgat      1740
     ttctgcagga aaaggagctg caatgatcac taacaagaag ccgaggattg tggatgtagc      1800
     aactccggga gttgttatta gtacctgatg ttggtttggt ttggtttggt tttgatgtac      1860
     aagttaaaat aaatgtgtgg ggcgttgtat tttggatgga gggtactaaa gcgtgtaatt      1920
     tgctgaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaa        1978
//