 |
|
EBI DbfetchID X67744; SV 1; linear; genomic DNA; STD; PRO; 3161 BP. XX AC X67744; XX DT 19-MAR-1993 (Rel. 35, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 53) XX DE S.xylosus scrB and scrR genes XX KW scrB gene; scrR gene; sucrase; sucrose repressor. XX OS Staphylococcus xylosus OC Bacteria; Firmicutes; Bacillales; Staphylococcus. XX RN [1] RX PUBMED; 8423155. RA Bruckner R., Wagner E., Gotz F.; RT "Characterization of a sucrase gene from Staphylococcus xylosus"; RL J. Bacteriol. 175(3):851-857(1993). XX RN [2] RC revised by [3] MAT RA Brueckner R.; RT ; RL Submitted (15-OCT-1992) to the EMBL/GenBank/DDBJ databases. RL R. Brueckner, Mikrobielle Genetik, Universitaet Tuebingen, Auf der RL Morgenstelle 28, 72076 Tuebingen , FRG XX RN [3] RP 1-3161 RA Brueckner R.; RT ; RL Submitted (16-AUG-1995) to the EMBL/GenBank/DDBJ databases. RL R. Brueckner, Mikrobielle Genetik, Universitaet Tuebingen, Auf der RL Morgenstelle 28, 72076 Tuebingen, FRG XX RN [4] RX PUBMED; 8550467. RA Gering M., Brueckner R.; RT "Transcriptional regulation of the sucrase gene of Staphylococcus xylosus RT by the repressor ScrR"; RL J. Bacteriol. 178(2):462-469(1996). XX DR CABRI; DSM 20267. XX FH Key Location/Qualifiers FH FT source 1..3161 FT /organism="Staphylococcus xylosus" FT /strain="DSM 20267" FT /isolate="cured strain C2a" FT /mol_type="genomic DNA" FT /clone_lib="genomic in E.coli" FT /clone="pRA2, pRA7" FT /db_xref="taxon:1288" FT -35_signal 427..432 FT /gene="scrR" FT -10_signal 450..455 FT /gene="scrR" FT mRNA 459..>1457 FT /gene="scrR" FT /note="459 is minor transcriptional start" FT mRNA 462..>1457 FT /gene="scrR" FT /note="462 is major transcriptional start" FT RBS 482..487 FT /gene="scrR" FT CDS 495..1457 FT /transl_table=11 FT /gene="scrR" FT /product="sucrose repressor" FT /db_xref="GOA:P74892" FT /db_xref="HSSP:1LQC" FT /db_xref="InterPro:IPR001761" FT /db_xref="UniProtKB/Swiss-Prot:P74892" FT /protein_id="CAA47969.1" FT /translation="MKNIADIAKIAGVSKSTVSRYLNNGSVSLKTQQKLDEIIRENDYQ FT PNQFAQSLRARRTNMIGAIIPRMNSFAVDETIKGVKTVCDQLNYSLLLNYTNLNIQLEI FT DALETFYRSKVDGIVFMATEITDQHLEVINKINVPVIIVGQAHDDLHCIIHNDYQAGYL FT VGDMLGQQGYNDIKFFGVTESDIAVGVQRKEGLIAGLEAHNIQPEISLTSFNYQEAMVD FT VVEALQAYPHYDAIVGATDSIALAIHKYNSEHKPHAHEKYIVGFGGDPVTDIVSPSIHT FT INYNFEYAGSVAMDKLNQMIQHQVIEQRIIIDVEQSFEN" FT misc_feature 504..553 FT /note="HTH-motif" FT -35_signal 1447..1452 FT /gene="scrB" FT -10_signal 1469..1474 FT /gene="scrB" FT mRNA 1482..>3022 FT /gene="scrB" FT /note="1482 is major transcriptional start" FT mRNA 1483..>3022 FT /gene="scrB" FT /note="1483 is minor transcriptional start" FT protein_bind 1487..1502 FT /gene="scrB" FT /bound_moiety="repressor protein" FT RBS 1526..1531 FT /gene="scrB" FT CDS 1541..3025 FT /transl_table=11 FT /gene="scrB" FT /product="beta-fructofuranosidase" FT /EC_number="3.2.1.26" FT /db_xref="GOA:Q05936" FT /db_xref="InterPro:IPR006232" FT /db_xref="UniProtKB/Swiss-Prot:Q05936" FT /protein_id="CAA47970.1" FT /translation="MSEWTKEQRYQKYEDVDQQTIEKLTEQVNQSEYRQTFHIQPQIGL FT LNDPNGLIYFKGNYYVSHQWFPLGPVHGLKYWYTYQSKDLVDFKAIGPTIKPDTKDDSH FT GVYSGSAFEYHDHLYYMYTANHRDSDWNRISTQHIAKMSKDGSINKFPKAVISAPPSGY FT TQHFRDPKVHVQDGVYYAMIAAQNIKKQGRILQYRSTDIVNWEFQGEVQTNLDDFGYMW FT ECPDYFNLNGYDMLLFCPQGIDSEGERFKNIYQSGYIMGQYDINNLTMNHADFHELDYG FT FDFYAPQTFLDENGQRILIGWMGLPDINYPSDADGWAHCLTIPRVLTIESGNLKQRPIK FT ALEKLRTNEETALGYANKFTRQLHPYEGKQFELIIDILENEATEVYFEVRTSKTESTLI FT TYNKREQKLTLDRSESGQLPEPVEGTTRSTYLDTPLSKLQLFVDTSSVEIFCNDGERVM FT TARIFTDENATGIKTSTESGQTYLKFTKYDLKGDTI" XX SQ Sequence 3161 BP; 1148 A; 460 C; 584 G; 969 T; 0 other; gaattcatag ctatgctttg agttcgacct ttttagtatt tatgattaat acataactgc 60 taattattac tggtaaaata ctcaataaat aaaaaaggtt caaagttatt agtagtaaga 120 gataacattt aagcaatgaa taaaaataaa tttattagat ttacctttat agatacatga 180 aataattaat atcattatag ggagaaaagc taaaaaagcg ctgattaact accattttta 240 actaaaaaaa gggcaaacaa atagcgatgc ttattaagcc atacaatttc acctaactca 300 tgtgatatcg cttctttaaa ttgtagtttc taattctata tcacactcga aaatcaccct 360 atatattgct gtttagatac aaagctgaaa aattcaatct tttggtaaga gaagatttaa 420 atagtattta aagcgattgc attatgataa aataagtatt gtttataata gttaattaat 480 gagagtgaga aaatatgaag aacattgctg atatagcaaa aattgctggg gtttcaaaaa 540 gtacagtctc gagatattta aataatggtt cggtaagttt aaaaactcaa caaaaattag 600 atgaaattat tcgcgaaaat gactatcagc ctaatcaatt tgcacaaagc ttacgtgcgc 660 gtagaactaa tatgataggg gcaattatcc ctagaatgaa ctcttttgct gtcgacgaaa 720 caattaaagg agtcaaaaca gtctgtgatc aattgaatta tagtttactt ctaaattaca 780 caaatcttaa tattcaatta gaaattgacg cgctagaaac attttataga agtaaggtag 840 atggtattgt ctttatggca acagaaatca cagaccaaca tttagaagtt attaataaaa 900 taaatgttcc tgtaattatt gttggtcaag cacatgatga tttacattgt attattcata 960 atgattacca agctggatac ttagttggtg atatgttagg tcaacaaggt tataacgata 1020 ttaaattctt tggtgtaaca gaaagtgata ttgcggtagg tgttcaacgc aaagaaggtc 1080 tgattgcggg tttagaagca cataacatac aacctgaaat ttcgctcaca tcatttaatt 1140 atcaagaggc tatggtcgat gttgtagaag cgttacaagc ataccctcat tatgacgcaa 1200 ttgttggggc tactgattca attgccttag cgatacataa atataattca gaacacaagc 1260 cacatgcgca tgaaaagtat attgtaggat ttggtggaga tccagtcact gatattgtgt 1320 caccatctat ccatactata aattacaatt ttgaatatgc gggcagtgtg gctatggaca 1380 aactaaatca gatgatacaa catcaagtaa ttgaacaacg cattattatc gatgttgaac 1440 agtcatttga aaattaaaag aaaaatggta tgataaaaag tatattatgg aaccggtacc 1500 atttaaatga gcttatttta taaataggag gatacgcacc atgtcggagt ggacaaaaga 1560 gcaacgttat caaaaatatg aagatgttga tcaacaaacg attgaaaaat taactgaaca 1620 agttaatcag tctgaatata gacaaacttt tcatatacaa ccccaaatag gattgttaaa 1680 tgatcctaat ggtcttatct atttcaaggg gaattattat gtatctcatc aatggtttcc 1740 tttaggacct gtgcacggat tgaaatattg gtatacatat caaagtaagg atcttgtaga 1800 ttttaaagct attggtccaa cgattaaacc agatacgaaa gatgatagtc acggtgtata 1860 tagtggcagt gcctttgaat atcatgacca cttatattat atgtatacag cgaaccatcg 1920 agatagtgat tggaatagaa tttctacaca acatatcgca aaaatgtcta aggatggatc 1980 gattaataaa tttcctaaag cagtgatatc agcaccaccg tctggttata ctcaacattt 2040 tagggatcct aaggtacatg ttcaagacgg cgtttattac gcaatgatag ctgctcaaaa 2100 tattaaaaaa caagggagaa ttctgcaata tcgttcaaca gatatcgtca attgggagtt 2160 tcaaggtgag gtccaaacga atctagatga ttttggttat atgtgggagt gtccggatta 2220 ttttaattta aacggttatg acatgttatt attttgtcct caaggtatag attcagaagg 2280 ggaacgcttt aagaatattt atcaatctgg gtatattatg gggcagtatg atattaataa 2340 tttaactatg aaccatgctg attttcatga attagattat ggttttgatt tttatgctcc 2400 tcagacattc ttagacgaaa atggtcaacg catattaatt gggtggatgg gtttgcccga 2460 tattaactat ccttcagatg ccgatggttg ggcacattgt ttaactatcc cacgtgtatt 2520 gacaattgag tcaggtaatt taaaacaacg tccgatcaaa gcgcttgaaa aacttcgcac 2580 aaatgaagaa acggcactag gttatgctaa taaattcact agacaattac atccatatga 2640 aggtaaacag tttgaattaa tcatcgatat tttggaaaat gaggcgacag aagtatactt 2700 tgaagtacgt acatctaaga cggaatctac attgattacg tataataaga gggagcagaa 2760 attaacatta gatcgcagtg aaagtggtca attaccagaa cctgttgaag gtacaacgag 2820 gagtacttat ttagatacgc cattatcaaa actgcagtta tttgtagata cttctagtgt 2880 tgaaatattc tgtaatgatg gcgaacgtgt gatgacagcc agaatattta cagatgaaaa 2940 tgcaacaggc attaaaacat ctactgaatc aggccaaacc tatctaaaat ttacaaaata 3000 cgatttgaaa ggtgacacta tatgaaacgt ctactagcaa ttggagaggc tttaatagat 3060 tttataccga atactacaga ttctaaatta aaagatgtag aaaagtttac tcgacaagtc 3120 gggggtgcac catgtaatgt ggcttgtaca gcgacaaaac g 3161 // |
|