spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X67712; SV 1; linear; genomic DNA; STD; PRO; 1280 BP.
XX
AC   X67712;
XX
DT   14-OCT-1992 (Rel. 33, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 11)
XX
DE   P.immobilis lip gene for lipase
XX
KW   lipase; triacylglycerol acyl hydrolase.
XX
OS   Psychrobacter immobilis
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Moraxellaceae; Psychrobacter.
XX
RN   [1]
RX   DOI; 10.1016/0167-4781(93)90078-R
RX   PUBMED; 7916627.
RA   Arpigny J.L., Feller G., Gerday C.;
RT   "Cloning, sequence and structural features of a lipase from the antarctic
RT   facultative psychrophile Psychrobacter immobilis B10";
RL   Biochim. Biophys. Acta 1171(3):331-333(1993).
XX
RN   [2]
RP   1-1280
RA   Arpigny J.;
RT   ;
RL   Submitted (25-AUG-1992) to the EMBL/GenBank/DDBJ databases.
RL   J. Arpigny, University of Liege, Laboratory of Biochemistry, Institute of
RL   Chemistry B6, Sart Tilman, 4000 Liege, BELGIUM
XX
RN   [3]
RX   DOI; 10.1016/0167-4781(95)00086-V
RX   PUBMED; 7632728.
RA   Arpigny J.L., Feller G., Gerday C.;
RT   "Corrigendum to "Cloning, sequence and structural features of a lipase from
RT   the antarctic facultative psychrophile Psychrobacter immobilis B10"
RT   [Biochim. Biophys. Acta 1171 (1993) 331-333]";
RL   Biochim. Biophys. Acta 1263(1):103-103(1995).
XX
RN   [4]
RA   Arpigny J.L., Gerday C., Lamotte J.;
RT   "Molecular adaptation to cold of an Antarctic bacterial lipase";
RL   J. Mol. Catal., B Enzym. 3:29-35(1997).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1280
FT                   /organism="Psychrobacter immobilis"
FT                   /strain="B10; antarctic"
FT                   /mol_type="genomic DNA"
FT                   /clone="pLIPI1"
FT                   /cell_line="E.coli RR1"
FT                   /db_xref="taxon:498"
FT   CDS             244..1197
FT                   /transl_table=11
FT                   /gene="lip"
FT                   /product="triacylglycerol lipase"
FT                   /EC_number="3.1.1.3"
FT                   /db_xref="GOA:Q02104"
FT                   /db_xref="InterPro:IPR000073"
FT                   /db_xref="InterPro:IPR000639"
FT                   /db_xref="UniProtKB/Swiss-Prot:Q02104"
FT                   /protein_id="CAA47949.1"
FT                   /translation="MLLKRLCFAALFSLSMVGCTNAPNALAVNTTQKIIQYERNKSDLE
FT                   IKSLTLASGDKMVYAENGNVAGEPLLLIHGFGGNKDNFTRIARQLEGYHLIIPDLLGFG
FT                   ESSKPMSADYRSEAQRTRLHELLQAKGLASNIHVGGNSMGGAISVAYAAKYPKDVKSLW
FT                   LVDSAGFWSAGIPKSLEGATLENNPLLIKSNEDFYKMYDFVMYKPPYLPKSVKAVFAQE
FT                   RIKNKELDAKILEQIVTDNVEERAKIIAQYKIPTLVVWGDKDQIIKPETVNLIKKIIPQ
FT                   AQVIMMEDVGHVPMVEALDETADNYKAFRSILEAQR"
FT   -35_signal      191..195
FT   -10_signal      213..218
XX
SQ   Sequence 1280 BP; 391 A; 246 C; 282 G; 361 T; 0 other;
     tgatacgcaa gttcgcgata aaaacaatca attactaaaa tttttattaa atgagtttga        60
     ggtgcgctat cacattatca gcggcaacta taatgaaagg tataaagaat cgcttaaata       120
     tgttgataaa ttattgaccc gtaacctagt tattaatatt cttatgctaa gattgcgatt       180
     ttaagcgtct ttaacttcta cccttttcta cctatactca ccaactatca caaggatgtt       240
     tcgatgctct taaaacgcct gtgctttgcc gcgcttttta gtctttctat ggtcggttgt       300
     accaacgcgc ccaatgcgtt agcggtcaat accacccaaa agattattca atatgagcgt       360
     aacaagtctg atttagaaat caaatcgtta acactagctt ctggtgataa gatggtctat       420
     gcagaaaacg gcaatgtcgc tggcgagccg ttattactca ttcatggctt tggcggtaat       480
     aaagacaact ttacccgtat cgcgcgtcag ttagaaggct atcatctgat tattcctgat       540
     ttgcttggtt ttggtgaatc tagtaaaccg atgagcgctg actatcgctc ggaggcgcaa       600
     cggacgcgct tgcatgaatt gctgcaagcg aaaggtttgg cgtcaaatat tcatgtcggc       660
     ggcaactcga tgggcggcgc aatcagtgtg gcttatgcgg cgaagtatcc caaagacgtc       720
     aaaagtttat ggctcgtcga tagcgcaggt ttttggtcgg caggtattcc taaatcatta       780
     gaaggcgcaa cgttagagaa caatccgctg ttaattaaga gcaatgaaga cttctataag       840
     atgtatgatt tcgtcatgta taagccgcct tatctgccaa aatcagtcaa agcggtgttt       900
     gcccaagagc gtatcaaaaa taaagagctg gacgccaaaa tcttggagca aatcgtcacc       960
     gataatgtcg aagagcgcgc caaaatcatc gcacaatata aaattccaac gctagtggta      1020
     tggggtgata aagatcagat catcaaacct gaaacggtca atctcataaa aaaaatcatc      1080
     ccgcaagcac aagtgattat gatggaagat gtcggtcatg taccgatggt tgaagcgctg      1140
     gatgaaacgg cagataatta taaggcgttt agaagtatat tagaggcgca gcgctaggga      1200
     ttggttgaaa gtcattattt taaaatcaaa aaagaaacac tagatacgtt ggtgtttctt      1260
     ttttttggct acaagatgtt                                                  1280
//
spacer
spacer