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EBI DbfetchID X65616; SV 1; linear; genomic DNA; STD; PRO; 3791 BP. XX AC X65616; S51648; XX DT 07-MAY-1992 (Rel. 31, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 27) XX DE S.reticuli cel1 gene for mycelium-associated cellulase (avicelase 1) XX KW avicelase; cellulase; mycelium-associated cellulase; ORF1. XX OS Streptomyces reticuli OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Streptomycineae; Streptomycetaceae; Streptomyces. XX RN [1] RP 1-2760 RA Schlochtermeier A.; RT ; RL Submitted (15-APR-1992) to the EMBL/GenBank/DDBJ databases. RL A. Schlochtermeier, AGM FB Biologie/Chemie, Universitaet Osnabrueck, RL Angewandte Genetik der Mikroorganismen, Barabarastr. 11, D W-4500 RL Osnabrueck, FRG XX RN [2] RP 1-2760 RX DOI; 10.1111/j.1365-2958.1992.tb01797.x RX PUBMED; 1282194. RA Schlochtermeier A., Walter S., Schroder J., Moorman M., Schrempf H.; RT "The gene encoding the cellulase (Avicelase) Cel1 from Streptomyces RT reticuli and analysis of protein domains"; RL Mol. Microbiol. 6(23):3611-3621(1992). XX RN [3] RP 1-3791 RA Walter S.; RT ; RL Submitted (23-FEB-1995) to the EMBL/GenBank/DDBJ databases. RL Walter S., AGM FB Biologie/Chemie, Universitaet Osnabrueck, Angewandte RL Genetik der Mikroorganismen, Barabarastr. 11, D W-4500 Osnabrueck, FRG XX RN [4] RP 1-3791 RX PUBMED; 7574585. RA Schlochtermeier A., Walter S.; RT "Studies of Streptomyces reiculi cel1 (cellulase) gene expression in RT Streptomyces strains, Eschericia coli and Bacillus subtilis"; RL Appl. Environ. Microbiol. 61:487-494(1995). XX FH Key Location/Qualifiers FH FT source 1..3791 FT /organism="Streptomyces reticuli" FT /strain="described by Zaehner, Tuebingen" FT /mol_type="genomic DNA" FT /clone_lib="lambda-charon 35" FT /clone="CCEL30, pUCEL1" FT /db_xref="taxon:1926" FT CDS 36..617 FT /transl_table=11 FT /gene="orf1" FT /db_xref="InterPro:IPR010499" FT /db_xref="UniProtKB/TrEMBL:Q54993" FT /protein_id="CAA46569.1" FT /translation="MLRLRQAELEAGMAAAAGRLVQVEARLRSIESEGHMPTNDVVVKN FT VPAIRVAELTATAASFAPEHIGPVYGPLYDELFRRLDAAGVTPTGPGVAFYEDAPEGGG FT RIVVHAAVQVAAPLQDGAFRVLDLPPVDQAATIVHRGSMDAVLPTAQALARWIDGNGYR FT SAGYPRDINLECPENRDDWVTELQAPVAKP" FT RBS 23..26 FT terminator 629..664 FT /note="putative" FT RBS 1323..1326 FT sig_peptide 1335..1421 FT /gene="cel1" FT CDS 1335..3575 FT /transl_table=11 FT /gene="cel1" FT /product="avicelase 1" FT /db_xref="GOA:Q05156" FT /db_xref="HSSP:1GU3" FT /db_xref="InterPro:IPR013783" FT /db_xref="UniProtKB/Swiss-Prot:Q05156" FT /protein_id="CAA46570.1" FT /translation="MKRRTTAVLTLTALLGTALTALPVQQAGAEEVEQVRNGTFDTTTD FT PWWTSNVTAGLSDGRLCADVPGGTTNRWDSAIGQNDITLVKGETYRFSFHASGIPEGHV FT VRAVVGLAVSPYDTWQEASPVLTEADGSYSYTFTAPVDTTQGQVAFQVGGSTDAWRFCV FT DDVSLLGGVPPEVYEPDTGPRVRVNQVAYLPAGPKNATLVTDATARLPWQLRNAQGTTV FT ARGLTVPRGVDASSGQNVHSIDFGSYRGRGTGYTLVADGETSHPFDIDAAAYRPLRLDS FT VKYYYTQRSGIAIRDDLRPGYGRAAGHLNVAPNQGDANVPCQPGVCDYTLDVTGGWYDA FT GDHGKYVVNGGIATWELLSTYERSLTARTGHPAALGDGTLALPESGNKVPDVLDEARWE FT LEFLLKMQVPAGQPLAGMAHHKLHDEQWTGLPLLPDQDPQKRELHPPTTAATLNLAATA FT AQAARLYRPFDKAFAARALTAARTAWQAALAHPDLLADPNDGTGGGAYNDDDVTDEFYW FT AAAELYLTTGERQFADHVLDSPVHTADIFGPTGFDWGHTAAAGRLDLALVPSRLPGRDQ FT VRRSVIKAADTYLATLTAHPYGMPYAPAGNRYDWGSSHQVLNNGVVLASAYDLTGAAKY FT RDGALQGMDYVLGRNALNMSYVTGYGEVSSHNQHSRWYAHQLDPTLPNPPSGTLAGGPN FT SSIQDPYAQSKLTGCVGQFCYIDDIQSWSTNETAINWNAALARMASFAADQG" FT mat_peptide 1422..3575 FT /gene="cel1" FT /product="avicelase 1" XX SQ Sequence 3791 BP; 589 A; 1486 C; 1255 G; 461 T; 0 other; gtcgcgagga ggatcagcac cgaggaactg cgcgcatgct gcggctgcgg caggccgagc 60 tggaggccgg catggccgcc gcggccggac ggctggtcca ggtcgaggcg aggctccggt 120 cgatcgagag cgaggggcac atgcccacga acgacgtcgt cgtcaagaac gtcccggcga 180 tccgggtggc ggaactcacc gcgaccgccg cgagcttcgc gcccgagcac atcggcccgg 240 tctacggacc gctctacgac gagctgttcc ggcgcctgga cgcggcgggc gtcacgccca 300 cgggccccgg tgtcgccttc tacgaggacg cgccagaggg cggcggcagg atcgtcgtcc 360 acgccgccgt ccaggtcgcg gcgccccttc aggacggcgc gttccgggtg ctcgacctgc 420 cacccgtcga ccaggcggcg acgatcgtgc accgcggctc gatggacgcg gtcctgccca 480 cggcgcaggc cctggcccgc tggatcgacg gcaacggcta ccggtcggcc ggatacccgc 540 gggacatcaa cctggagtgc cccgagaacc gcgacgactg ggtcaccgaa ctccaggcac 600 cggtggccaa gccctgagcc gcggcggtcc tggccggttc ggtgacctcc ccgagccggt 660 caggtgccga ggggccccgg ttcgcgccgg cacgaccgcc gcaggcgagc cacctcaccg 720 cgcgtcggcc gaccctccgt cacaagccct cacggcgcgg gttcgcgtcc gggctcccgg 780 gccagtcgct cctggacgtc caggcgtgcg cactcgtagt gcgcgccgtt ctgccggagc 840 accccgagtg cgtgactcct cgtcaccgtc ggccagtcgg accacggacg cccctgccgg 900 acgaggacca gcgccgcctc cgctcccggt cgggcgcggc ctgtacgcac gtcgctccac 960 cggccggtga gccacggcca ctccggacag ccaaggtgag ggagccggag cgccgcgcat 1020 tcgaccgaaa tgcaacgaac aggaatcgaa tacgctgtgg gccgtgctct ctcgatactc 1080 tcgtccgcct ctgtgactga gcgccgcagt cggcccttga gcacgccggc gggcacctga 1140 gcgcgacacc ggcccacagg ggccggctgg gggagaaacc gcctgcgacg atccgggaag 1200 gcttcactgg gtccaaatcc gtcgcgtccg gaccgttgac ggggtgtcat gcgcgcagca 1260 agcatgtctc gcgtccgagt tttgggagcg ctcccacgca cctggcgcac ccgcacgtcc 1320 cgaggagccc agacgtgaaa cgacgcacaa ccgccgtgct gaccctcacc gccctgctgg 1380 gcacggcgct gaccgcgctg cccgtgcagc aggccggtgc cgaggaggtc gagcaggtca 1440 ggaacggcac cttcgacacc accaccgacc cctggtggac cagcaacgtc accgcgggcc 1500 tgagcgacgg ccggctgtgc gcggacgtgc ccggcggcac caccaaccgc tgggactccg 1560 cgatcgggca gaacgacatc acgctggtga agggggagac gtaccggttc tccttccacg 1620 cctccggcat ccccgagggg cacgtcgtgc gggcggtcgt gggcttggcc gtgtcgccgt 1680 acgacacctg gcaggaggcg agcccggtgc tcaccgaggc cgacggttcg tactcctaca 1740 ccttcaccgc gcctgtcgac accacccagg ggcaggtcgc cttccaggtc ggcggcagca 1800 ccgacgcctg gcggttctgc gtggacgacg tctccctgct cggcggggtg cccccggagg 1860 tctacgagcc ggacaccgga ccccgggtgc gcgtcaacca ggtcgcctac ctgcccgccg 1920 ggccgaagaa cgccaccctc gtcaccgacg ccaccgcccg gctgccctgg cagctgagga 1980 acgcccaggg cacgacggtc gcccggggcc tcaccgtgcc gcgcggcgtg gacgcctcct 2040 ccgggcagaa cgtccactcg atcgacttcg gctcctaccg cgggcgcggc accggctaca 2100 ccctcgtcgc cgacggcgag accagccacc cgttcgacat cgacgcggcg gcctaccgcc 2160 cgctgcggct ggactccgtg aagtactact acacgcagcg cagcggcatc gcgatccgcg 2220 acgacctgcg gcccggctac ggccgcgcgg ccggccacct gaacgtcgcc cccaaccaag 2280 gcgacgcgaa cgtcccctgc cagccgggcg tgtgcgacta caccctcgac gtcaccggcg 2340 gctggtacga cgccggcgac cacggcaagt acgtcgtgaa cggcggcatc gccacctggg 2400 aactgctgag cacctacgag cggtcgctga cggcccgcac cggccacccg gccgccctcg 2460 gcgacggcac cctcgcgctg cccgagagtg gcaacaaggt gccggacgtc ctcgacgagg 2520 cccgctggga gctggagttc ctgctgaaga tgcaggtgcc cgccgggcag ccgctggccg 2580 ggatggccca ccacaagctc cacgacgagc agtggaccgg tctgcccctg ctgcccgacc 2640 aggacccgca gaagcgggag ctgcacccgc cgaccaccgc ggcgaccctc aacctggccg 2700 cgacggccgc ccaggccgcc cggctgtacc ggcccttcga caaggcgttc gccgcacggg 2760 cgctgacggc cgcccgcacg gcctggcagg ccgctctcgc ccaccccgac ctcctcgccg 2820 accccaatga cggcaccgga ggcggcgcct acaacgacga cgacgtcacc gacgagttct 2880 actgggccgc ggccgagctc tacctgacca cgggtgagcg gcagttcgcc gaccatgtgc 2940 tcgactcccc ggtgcacacc gcggacatct tcggcccgac cggcttcgac tggggccaca 3000 cggccgccgc cggccggctc gacctcgccc tcgtgcccag ccggctgccc ggccgcgacc 3060 aggtgcgccg ctcggtgatc aaggccgccg acacctacct cgccacgctg accgcccacc 3120 cctacggcat gccgtacgcc cccgccggta accgctacga ctggggctcc agccatcagg 3180 tcctcaacaa cggcgtggtc ctcgccagcg cctacgacct caccggcgcc gccaagtacc 3240 gcgacggggc gctgcagggc atggactacg tcctcgggcg caatgccctg aacatgtcct 3300 acgtcaccgg ctacggcgag gtcagctccc acaaccagca cagccgctgg tacgcccacc 3360 agctcgaccc caccctgccg aacccgccgt ccggcaccct cgccggcggt cccaactcga 3420 gcatccagga cccgtacgcc cagtccaagc tcacgggctg cgtcggccag ttctgctaca 3480 tcgacgacat ccagtcctgg tcgaccaacg agaccgcgat caactggaac gcggccctcg 3540 cccgcatggc ctccttcgcg gccgatcagg ggtgaccgcc cggcacgcct gaggcaccgc 3600 tcgggtaccg cactgccctg gggacactta cgggccagta cccgagcggt accgccagcc 3660 ctcgatatgg agttcagcca tcgcgagccg gagggcctgc ggcagatcgc caaggagcgc 3720 ggtacgccga tgaaggcgct catgcgcgag gcggccgccg ccggcacagc gcgccaccgg 3780 gcgtgcggga g 3791 // |
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