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EBI DbfetchID X63438; SV 1; linear; genomic DNA; STD; PRO; 3382 BP. XX AC X63438; S47845-S47846; XX DT 13-OCT-1992 (Rel. 33, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 9) XX DE D.ambivalens lig gene for DNA ligase XX KW DNA ligase; lig gene; thermoacidophile. XX OS Acidianus ambivalens OC Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; OC Acidianus. XX RN [1] RP 1-3382 RA Kletzin A.; RT ; RL Submitted (06-DEC-1991) to the EMBL/GenBank/DDBJ databases. RL A. Kletzin, Max-Planck-Institut f Biochemie, Am Klopferspitz 18a, D-8033 RL Martinsried, FRG XX RN [2] RP 1-3382 RX DOI; 10.1093/nar/20.20.5389 RX PUBMED; 1437556. RA Kletzin A.; RT "Molecular characterisation of a DNA ligase gene of the extremely RT thermophilic archaeon Desulfurolobus ambivalens shows close phylogenetic RT relationship to eukaryotic ligases"; RL Nucleic Acids Res. 20(20):5389-5396(1992). XX FH Key Location/Qualifiers FH FT source 1..3382 FT /organism="Acidianus ambivalens" FT /strain="DSM 3772" FT /mol_type="genomic DNA" FT /db_xref="taxon:2283" FT CDS complement(250..771) FT /transl_table=11 FT /gene="ORF3" FT /db_xref="GOA:Q02103" FT /db_xref="HSSP:1OGH" FT /db_xref="InterPro:IPR008180" FT /db_xref="UniProtKB/Swiss-Prot:Q02103" FT /protein_id="CAA45033.1" FT /translation="MILGDRDLKYYLEKGWIVISPLTQDTIRENGVDLRVGGEIARFKK FT TDEIYEDGKDPRSFYEIEKGDEFIIYPNEHVLLVTEEYVKLPNDVMAFVNLRSSFARLG FT LFVPPTIVDAGFEGQLTIEVLGSAFPVKIKRGTRFLHLIFARTLTPVENPYHGKYQGQQ FT GVTLPKFKFR" FT CDS 908..2710 FT /transl_table=11 FT /gene="lig" FT /product="DNa ligase" FT /db_xref="GOA:Q02093" FT /db_xref="InterPro:IPR012340" FT /db_xref="UniProtKB/Swiss-Prot:Q02093" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA45034.1" FT /translation="MEFKIIAEYFDRLEKISSRLQLTSLLADLFKKTDKNVIDKVVYII FT QGKLWPDFLGMPELGIGEKFLIRALSIATSVSDDEIEKMYKSVGDLGQVAFDIKQKQQS FT ASILAFLGAQKASKPLTVEKVYDDLAKVATSTGEGSRDIKIRLLAGLLKDASPLEAKYL FT VRFVDGRLRVGIGDATILDALAITFGGGQNFRPIVERAYNLRADLGNIAKILANGGIEQ FT LKNIKPQPGIPIRPMLAERLSDPAEMLSKVGNIALVDYKYDGERGQIHKAGDKIFIFSR FT RLENITNQYPDVAEYISKYVKGNEFIVEGEIIPVDPETGEMRPFQELMHRKRKSDIHEA FT IKEYPVNVFLFDLMYYEGEDYTVKPLSERRKKLESIVEDNDYVHIATHIITDNVEKLKE FT FFYQAISEGAEGVMVKSLAPDAIYQAGSRGWLWIKFKRDYQSEMADTVDLVMVGAFHGK FT GRKGGKYSSFLMAAYNPDKDVFETVCKVASGFTDAELDDLQKKIAELKRDTPHPRVVST FT MVPDVWLTPALVAEIIGAEITISPLHTCCKDQYAEGGLSIRFPRFIRWRPDKSPEDATT FT NREILEMYKSQLKKIEEKPSDQSV" FT CDS 2961..>3382 FT /transl_table=11 FT /gene="ORF2" FT /db_xref="UniProtKB/Swiss-Prot:Q02102" FT /protein_id="CAA45035.1" FT /translation="MGISYGVKIAIEDEKIELVQADHVFGAAQVVVTNSNGESVGYTGD FT FKNPGKGTPILNTDVLIVDTTYGKPTFRRKFRDEVEVLFSDYVNDSLIYGPVRVYAYYG FT KIQEAMKILRKNGISAPFIVDGKIKEVTDIAIKYGL" FT misc_feature 878..883 FT /note="lig box A" FT misc_feature 906..909 FT /note="lig box B" FT /experiment="experimental evidence, no additional details FT recorded" XX SQ Sequence 3382 BP; 1188 A; 512 C; 683 G; 999 T; 0 other; aagcttaaga atcctgactt attctttgaa aaaattgaaa agcaaggtgg gaaagttcta 60 tatactgata aagaaagaat agccgtagat gtcgatttct ttaataattt tgtaaagaaa 120 ctttcagata ttcacacttc agatgatgta gaagcgcaaa aatacctcac taagcaagaa 180 cataaattat tccaaaaatt gagacaattg ggaataatat acttcgataa taatactaag 240 gcatggaagt tatcggaatt taaattttgg taaagttacg ccttgttgtc cttggtattt 300 gccatgataa ggattctcta ctggtgttaa tgttctggcg aaaatgaggt gaagaaagcg 360 agtccccctc tttatcttca cgggaaaagc agaacctagt acttctattg tgagttgtcc 420 ttcaaagcct gcatctacga tagtcggtgg tacaaaaaga cccagcctgg caaaagaaga 480 ccttaagttg acgaaagcca ttacgtcgtt cggcagttta acatactcct cagtaactaa 540 cagtacatgt tcgttagggt aaatgatgaa ttcatctcct ttctctatct cataaaatga 600 acgcgggtct tttccatctt catatatttc gtcagtcttc ttaaagcggg ctatctctcc 660 tcctactctt aaatcgaccc cgttctctct tatagtatct tgagtaagag gagagattac 720 tatccaaccc ttttctaggt agtattttaa atctctatct ccaagaatca taaatatcta 780 ctatttttca tcttgcaaga atatatttta taaggaaaat ggcttcagag taactctgat 840 acttcatcct tctggcttgc cggttttcat catcaaatat attatatcgt aattcaaact 900 tttatttatg gagtttaaaa ttatagctga atactttgat agactagaaa agatctcttc 960 taggcttcaa cttacatcat tacttgcaga tctttttaag aaaactgaca aaaatgttat 1020 tgataaagtt gtatatataa ttcagggtaa actatggcca gacttcttag gaatgccaga 1080 attaggaata ggtgaaaaat tccttataag agcattatca atagctacaa gcgtaagcga 1140 tgatgaaata gaaaaaatgt ataaatcagt aggagatttg gggcaagtag cttttgatat 1200 aaagcaaaaa cagcagtctg ccagtatttt agcgttttta ggagcgcaaa aagcttcaaa 1260 gccgctaact gtagaaaaag tttatgatga tttagcaaaa gttgctacaa gcactggaga 1320 aggtagtaga gatattaaaa tcagactatt agcaggatta cttaaggatg cttctccact 1380 tgaggctaaa tatttagtta gatttgttga tggtaggtta agggttggaa taggagacgc 1440 tacaatatta gacgcactag caataacttt cggcggcggg cagaacttta ggcctatagt 1500 tgaaagagca tacaatttga gggccgattt aggtaatatt gcaaaaatat tagcaaatgg 1560 cggaattgaa caactgaaga acattaaacc tcagccaggg attccaataa ggccaatgct 1620 agctgaaaga ttatcagacc ctgcagagat gttaagtaag gtaggcaata ttgcattggt 1680 cgattataag tatgatggag agagaggaca aattcataaa gcaggagata aaattttcat 1740 attctctaga agattggaaa atataacaaa tcaatatcct gacgtagcag agtatatttc 1800 aaaatacgtt aaaggtaatg agttcatagt tgagggagaa ataataccag tagatccaga 1860 aactggtgaa atgagacctt tccaagaatt gatgcacagg aaaaggaagt ccgatattca 1920 cgaggctata aaagagtatc cggttaacgt attcttattt gatttaatgt actatgaagg 1980 agaagattat acagtaaagc cgttatcaga aagacgtaag aagttagaat caatagttga 2040 agataacgat tatgtacata tagccactca tattataact gataatgtag aaaaactaaa 2100 ggaattcttc tatcaagcaa tctctgaagg cgcagaaggc gtaatggtaa aatctctggc 2160 accagatgca atatatcaag caggatcaag aggttggctt tggataaagt ttaagagaga 2220 ttaccagagc gaaatggcag atactgtaga cctggtaatg gtaggtgctt ttcacggtaa 2280 aggaaggaaa ggaggcaaat atagttcctt cttaatggct gcatataatc cagacaagga 2340 cgttttcgag actgtatgta aagttgcttc tggttttact gatgcagaac ttgacgattt 2400 acagaagaaa attgccgagt taaaaagaga cacacctcat ccaagagttg tatcaaccat 2460 ggttccagat gtttggttaa caccagcact tgttgccgaa ataattggag cagaaataac 2520 tatatctcct ctacatactt gttgcaaaga tcagtatgca gaaggtggtt tatcaataag 2580 attcccaaga tttataaggt ggagaccaga taagagtcca gaagatgcaa ctactaatcg 2640 tgaaattctt gagatgtata agagccaatt aaagaagatt gaagaaaagc cgtcagatca 2700 gagtgtataa gtttttataa aataactcat agttatgttt tgtgttcttc gatattaaga 2760 agtctggtgc aatactttta ggtaacaact ttacagtaga tggacattat gagcgaaatt 2820 ttagggttgt aactcatttt catgctgacc atattttgca attaaataag agcgtatcat 2880 cttgtattgg gattatatca actcctccaa ctcttgaagt tctctcagtt ttagggtatg 2940 atatacctaa gaaaaaagca atgggaataa gttatggtgt aaaaattgca atagaagatg 3000 agaaaataga actagttcag gcagatcatg tatttggagc cgctcaagta gttgtaacaa 3060 attccaatgg cgaaagtgta ggttatactg gagattttaa gaatccgggt aaaggtactc 3120 ctatacttaa tactgacgta ttaattgttg atactaccta cggaaagccg acatttagaa 3180 gaaaatttag agatgaagtt gaagtcttat tttcagatta cgtaaacgat tcattaattt 3240 atggaccagt aagagtttat gcttactatg gaaagattca agaggcaatg aaaatactga 3300 ggaaaaatgg aatttcggcc ccttttatag tagatggtaa aataaaagaa gttacagata 3360 ttgcaataaa gtatggtcta ga 3382 // |
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