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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X63431; SV 1; linear; genomic DNA; STD; PLN; 1174 BP.
XX
AC   X63431;
XX
DT   22-JUN-1992 (Rel. 32, Created)
DT   09-SEP-2004 (Rel. 81, Last updated, Version 3)
XX
DE   R.mannii str1 gene for strictosidine synthase
XX
KW   alkaloid biosynthesis; str1 gene; strictosidine synthase.
XX
OS   Rauvolfia mannii
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Rauvolfia.
XX
RN   [1]
RP   1-1174
RA   Kutchan T.M.;
RT   ;
RL   Submitted (03-DEC-1991) to the EMBL/GenBank/DDBJ databases.
RL   T.M. Kutchan, Lehrstuhl f Pharmazeutische Biologie, Universitaet Muenchen,
RL   Karlstr 29, D-8000 Muenchen 2, FRG
XX
RN   [2]
RP   1-1174
RX   DOI; 10.1016/0003-9861(92)90746-J
RX   PUBMED; 1567228.
RA   Bracher D., Kutchan T.M.;
RT   "Strictosidine synthase from Rauvolfia serpentina: analysis of a gene
RT   involved in indole alkaloid biosynthesis";
RL   Arch. Biochem. Biophys. 294(2):717-723(1992).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1174
FT                   /organism="Rauvolfia mannii"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="lambda Charon 4A"
FT                   /cell_line="R.mannii cell suspension culture"
FT                   /db_xref="taxon:4062"
FT   CDS             <1..1031
FT                   /codon_start=3
FT                   /gene="str1"
FT                   /product="strictosidine synthase"
FT                   /db_xref="GOA:P68174"
FT                   /db_xref="InterPro:IPR004141"
FT                   /db_xref="UniProtKB/Swiss-Prot:P68174"
FT                   /protein_id="CAA45025.1"
FT                   /translation="KLSDSQTMALFTVFLLFLSSSLALSSPILKEILIEAPSYAPNSFT
FT                   FDSTNKGFYTSVQDGRVIKYEGPNSGFVDFAYASPYWNKAFCENSTDAEKRPLCGRTYD
FT                   ISYNLQNNQLYIVDCYYHLSVVGSEGGHATQLATSVDGVPFKWLYAVTVDQRTGIVYFT
FT                   DVSTLYDDRGVQQIMDTSDKTGRLIKYDPSTKETTLLLKELHVPGGAEVSADSSFVLVA
FT                   EFLSHQIVKYWLEGPKKGTAEVLVKIPNPGNIKRNADGHFWVSSSEELDGNMHGRVDPK
FT                   GIKFDEFGNILEVIPLPPPFAGEHFEQIQEHDGLLYIGTLFHGSVGILVYDKKGNSFVS
FT                   SH"
FT   polyA_signal    1122..1127
FT   polyA_signal    1141..1146
XX
SQ   Sequence 1174 BP; 352 A; 250 C; 242 G; 330 T; 0 other;
     ccaaactttc tgattcgcaa actatggcac tgttcaccgt cttccttctt ttcctctcct        60
     cttcgctcgc tctctcctct ccaatcttga aagagatttt gattgaggct ccttcctatg       120
     cccccaattc cttcaccttc gactcaacca acaaagggtt ctacacctcc gtccaagatg       180
     gccgagttat caagtacgaa ggacccaact ccggtttcgt cgacttcgcc tatgcatctc       240
     cctactggaa caaagcgttc tgtgagaaca gcacagatgc agagaaaaga cccttgtgtg       300
     ggaggacata tgatatttca tataacttgc aaaacaacca gctttacatt gttgattgct       360
     attatcatct ttctgtggtt ggttctgaag gtgggcatgc tacccaactc gccaccagcg       420
     ttgatggagt gccattcaag tggctctatg cagtaacagt tgatcagaga actgggattg       480
     tttacttcac cgatgttagc accttatatg atgacagagg tgtccaacaa attatggata       540
     caagcgataa aacaggaaga ctaataaagt atgatccctc caccaaagaa acaacactac       600
     tgttgaaaga gctacacgtt ccaggtgggg cagaagtcag tgcagatagc tcctttgttc       660
     ttgtggctga gtttttgagc catcaaattg tcaaatattg gctagaaggg cctaagaagg       720
     gcactgcgga ggttttagtg aaaatcccaa acccaggaaa tataaagagg aacgctgatg       780
     gacatttttg ggtttcctca agtgaagaat tagatggaaa tatgcacgga agagttgatc       840
     ctaaaggaat aaaatttgat gagtttggga acattcttga agttatccca ctcccaccac       900
     catttgcagg tgaacacttc gaacaaattc aagagcatga tggtttgctg tacattggaa       960
     ccctgttcca tggctctgtg ggcatattag tatatgataa gaagggaaat tcttttgttt      1020
     caagtcatta aattttccac gaaccagatt ggttttgttt ttgatttgta taacactctt      1080
     taaaggtttt gtattcgaat cacgtcatct cagcctcgag gaataagaaa aaagcagcag      1140
     aataacttct caaaaaaaaa aaaaaaaaaa aagg                                  1174
//
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