 |
|
EBI DbfetchID X62835; SV 1; linear; genomic DNA; STD; PRO; 7872 BP. XX AC X62835; XX DT 12-NOV-1991 (Rel. 30, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 8) XX DE B.acidocaldarius amy gene for amylase XX KW amy gene; amylase. XX OS Alicyclobacillus acidocaldarius subsp. acidocaldarius OC Bacteria; Firmicutes; Bacillales; Alicyclobacillaceae; Alicyclobacillus. XX RN [1] RP 1-7872 RA Hemila H.O.; RT ; RL Submitted (22-OCT-1991) to the EMBL/GenBank/DDBJ databases. RL H.O. Hemila, Institute of Biotechnology, Valimotie 7, 00380 Helsinki, RL FINLAND XX RN [2] RP 1-7872 RX PUBMED; 8254309. RA Koivula T.T., Hemilae H., Pakkanen R., Sibakov M., Palva I.; RT "Cloning and sequencing of a gene encoding acidophilic amylase from RT Bacillus acidocaldarius"; RL J. Gen. Microbiol. 139(10):2399-2407(1993). XX FH Key Location/Qualifiers FH FT source 1..7872 FT /organism="Alicyclobacillus acidocaldarius subsp. FT acidocaldarius" FT /strain="ATCC 27009" FT /mol_type="genomic DNA" FT /clone_lib="lambda gt-10" FT /db_xref="taxon:1388" FT -35_signal 3224..3229 FT -10_signal 3246..3251 FT RBS 3288..3294 FT /note="amy gene" FT CDS 3297..7202 FT /transl_table=11 FT /gene="amy" FT /product="amylase" FT /db_xref="GOA:Q06307" FT /db_xref="HSSP:1JI2" FT /db_xref="InterPro:IPR006047" FT /db_xref="InterPro:IPR006589" FT /db_xref="InterPro:IPR013781" FT /db_xref="InterPro:IPR013783" FT /db_xref="InterPro:IPR014756" FT /db_xref="InterPro:IPR017853" FT /db_xref="UniProtKB/TrEMBL:Q06307" FT /protein_id="CAA44638.1" FT /translation="MHVKWTGAASLATTIVMSAALPFAAFENTRSVGTERVLAATAADS FT TNASPPTGASAGGGTAGETYSNAVQIVSGNGFRAGNGATVVLAVNGLNLNPSSFKVVVS FT NSLNGVIDVTSDSVLTGPNSIALHLPAGDDGLGAGTYTVTVESGGDSVSTPSGQGLQVM FT PYTTADTIQWDGIYTSDGAMYVSDPNPSPGQEVTISLRAYSGNLTKVILNCWDTAQNKG FT FQVEMSPGRTFGPYQLWSATIPASNGGTIYYRFDIYDGTSFACLSGDGLHTSDDINNNF FT PLPVGTVTLSTLQANPGDTVTVSDPVGDFAGSQDQPNHTVIRFVNSSGETAATVNGTNA FT SWNSVQFTVPQSLPNGLYRVEIDTVAKDADGVVNVELDRSAELIVGPLPAWMQAYAHDS FT FQAFYRSPFGAVSTGTPITLRLRAPLSVKSATLRLWGAADQSGEIDLPMQKLQMSGDEL FT AQQTGVQDINDYTWWTVTIPAADVTTPGTMWYQFVTETDTGQVVYYDDNGAQLEGPGQV FT GLSSDGPSYQISVYERGFQTPDWLKHAVIYEIMPDRFYNGNIATEENPNTQKGIYVGAD FT GTESLGPIQFHENWDSPPYDPNIPPLSDPKIASLRGNGQWNIDFFGGDLQGIEDKLDYL FT KSLGVNTLYLMPVFEAESNHKYDTADYFKIDPGFGTQQDWLNLVQAAHAKGFHIILDGV FT FEDTGSDSVYFNKFGNFHSNGAWQAYLKNQPSLSPYYSWYVWTGNTSNPYDSWFQIDTL FT PLTDTSNPAYQRFVYGSDNSVARVWIREGADGWRLDSADNGNFNTAWWGGFRQAVKSID FT PNAAIIGEIWDNATNDNGTDWLTGSTFDSVMNYQFRNAVIDFFRGTYNDGNVQHHAVDA FT AGFNQELMRLYSEYPLQSFYSMMNLVDSQDTMRILTILENAPQPGDLSALQQDEYRPSP FT AAEQLGIERLKLVSDFQFSFPGDPTIFYGDEAGLTGYSDPLNRRTYPWDNQNLDLLNHY FT RKLGAIRNANPVLQTGDFTPLYAQGMVYAFARTIRNGRDVFGVPAEDATAIVAINNQNQ FT AITVTIPTDGTVADGSTMLDELNNQWYKVQNGGITLTLQSYQGAILVTPSDAPMAYLQE FT EDSQNEIAWTPVQGAIGYRVWRQNPNGQWVPFGPVLPATDLSVTVERDAYAQTFAVQAL FT FSASDHAQSPVSAPKTVSLAVDVPAVRLSQPIVSGRVVGDRAMVSITPVSGATQYVIYQ FT RQGDGSYAPVATVSTSGDSAAIGEVPAQGPANSPHATIRVTVPVPAGFSSVTYRVAAQN FT EDGQAVTNPLTLSLSKK" FT CDS 7332..>7872 FT /transl_table=11 FT /product="malE protein-homologue" FT /db_xref="GOA:Q06311" FT /db_xref="HSSP:1ELJ" FT /db_xref="InterPro:IPR006059" FT /db_xref="InterPro:IPR006060" FT /db_xref="UniProtKB/TrEMBL:Q06311" FT /protein_id="CAA44639.1" FT /translation="MGNRIDRCGRARVGGRTVAGCGTSNGGQNTSPSTSSSSAKGEASA FT LPKGETITVWSWQTGPELQDVKQIAAQWAKAHGDKVIVVDQSSNPKGFQFYATAARTGK FT GPDVVFGMPHDNNGVFAEEGLMAPVPSGVLNTGLYAPNTIDAIKVNGTMYSVPVSVQVA FT AIYYNKKLVPQPPQTWA" XX SQ Sequence 7872 BP; 1615 A; 2240 C; 2473 G; 1544 T; 0 other; cgttcctcgt gccgtccgaa gcgttcccga cgaatctgcg cggcaccgcc gcgggatctc 60 ggcggcggct ggcaagattg gcgcgttcct cggcgcgttc gtgctgccgg gtatcctcaa 120 agccgcgggc ttgagtgtca ccatgggcat gctggcgggc gtggcgctgc tcggggcgct 180 gctcaccctc cttgcggttc ccgagatgaa gcagagatcc ctcgaggaca ccgaggagat 240 ccaggtgtcg ccgcaagcca cgacgcatca cagcattgtc ggctgatgtc ggcgtgaggc 300 gtctgtcgcg aaagcgagga gaaaaaccat gaagcacgga ccggatatgc cagaggacgt 360 ctgcatgcgg tgcttggcgg tgctcggcga gccccagcgg ctgcgcatcc tccgggcgtt 420 ggccgagggc gagcagaccg cgggcgcgct gagcgacgcg ccttcggcgt gcgccaaaac 480 accctttccc atcacatgag gcagcttcgc gagcacgggc tggtggaggt gcgccgccat 540 ccgcacgacg agcgatttac gttttatcga ttgaacgggc gtcgactgcg ggccctctct 600 gaccgtcctg acggattggg ccgctcgcgc agacgcagag tgtaaacctc gggccgctgg 660 tcttcgagac ggcggcctcg gcacggagat cctccttcgg ccgtttgcgc gggtggtgat 720 tgtgggtacg ggtggaactg aggtggagag cgtgggcaaa gatatgcagc gcaagaatgg 780 cttgatattt tcacttccga aatgagcccc tgcggcacag ctcccaggtc catcgtacat 840 gagcgcgggt tgtggcacca gacgtttcac gcgtggatcg ccaccggaag aagtcctgcg 900 gggcgcctcg tcgtccaact gcgcggcgcc cgcaaggaca cgaatccact gcgcctcgac 960 gtgagcgccg cgggtcacct cgaggcggga gaatcgccgg aagagggtgt gcgggagatc 1020 gaggaggagc tcggccttca tgtccccgcg ggacgcctgc acaagctcgg cgtggtggca 1080 cacgaggtgc ggctcgggcc ggtgtgggac cgcgaatttc accatttgta tgttgccatc 1140 gtcccagatt tgtcgctcgg ggtcctgcgc ccagcggtcg acgagatcgc gggcatctac 1200 gaactctctg ttcgtgactt cttcgatctc gccttcggcc gccggcagga agctgtgctc 1260 accggttatc gcgtgcttcc gggccatttg caaaagacgc gcgcaccgcg cgtggacgga 1320 cttcgtgccg cgatcgcgcc cgtacctcga cctgctcgca catttcttcc tgcgcgaggg 1380 ctgatctcag tccacaaacg ggccgttgaa gatgggcgct ctcccacggg ttcgccgaac 1440 accatcacgg cgcgaagcga ctccgcgccg cacgggcgtg ggcacaaagc ctcgtcggcg 1500 gctcggtgcc agatgaagag atcgcccttc tgcatcggaa tctcttcctc gccaaggtgc 1560 cccgcgccgt cgagcacgaa gatgaagccc tgatgatgcg ctggggcctc gagggtgtag 1620 gagcgcccgc gaccatttca cgtcctggta cgtcatgggg cggtgcgtcc taagcggcag 1680 tccacagccc acaatccact tccgccgcac gccctgggca ggttcgtcca cgggcacgcg 1740 cgtcgggcgg gatagtcctc gtagcccgga tccatgccct tgtcgctcct cgggatgttg 1800 atccagagct gcagtccgct gtcgatcccg tccgtaccag gcatctcgga gtgcacaatc 1860 ccgcgccccg ccgtcacgtg ttgcgcgccc ccttcaggga tgacgctttg gtggcccgcg 1920 ctgtccgcgt cgccacggcg ccttgcaaca catacgtgat aatctcaaag ccccggtgcg 1980 ggtggtccgg gaaccatccg ggtgttggat ggaaaagtga tcgagcagga gaaacgggtc 2040 gatgtacggc gttgtgcgcg aagggaagat ccgcgcgtgt tcgtaaaaca tgaggggcgc 2100 gcgatgcgcg cccgggaact tcatccatgc cacgtgagtg cctcctctcg cttgcatccg 2160 ccttttggtc aggccagcgc gtctcgaagt ttctccgcaa tgcggacgag cgccttcgtc 2220 gcgccaggcg aaaggctgta aaactgtgcg aatccgtgga tcagatcttc gaagttctcg 2280 atctcgacct tgacgcccgc cttgttcagc gcttccgcgt aaagcttgcc gacgtcgcgc 2340 agcggatcgt actgcgccgt cgcgatgtac gccggaggca agccgctcaa gtccgggtag 2400 aggacgggtg aaaaccacgg atgcgtgagt tcctccaggc tgttcaagta ttgatcccgg 2460 aaccagagca tcatgccgcc ggtcaggaga tagccttccg cattttcttc gatagatgcg 2520 ggaggatgag ccggatcgta ccccgtggaa gggtagatga gcagctggaa cgcgatggcc 2580 ggcccgccgc gctctttggc aaggatgctc gtcacagcgg caagattccc tccggcgctg 2640 tctccgccga ccgcgatgcg ggctggatcg agatgaaagt ccgctgcgcg ctccgcgatc 2700 cactgaagcg cgtcgtaggc gtcttccacg gcggcaggga acttgtgctc cggcgccagg 2760 cggtagtcga cggagaacac gaccgcgcgg ccgtctttcg cgaggacgcg gcagacggga 2820 tcgtgcgtct cgaggtctcc gacgacccaa ccgccgccgt gataatacac gagcgcgggg 2880 tagggcggtt cgacgccctc cgggcggtac atgcgcacct tgagcgtgcg gccaggcaga 2940 tccatgtcaa actctcggac ctcggccacg ggctccttct tgacaggagg aaacagcgac 3000 tgttgggaac gaaattgctg ggcggagaga tgtttgtagt ccggggcagg catgcggttg 3060 agttgatcga gcacctgctg aatgacggga tcgagcggca tgtgggtcgc ctcctctgcg 3120 atgggacatc tcacttacat tttaacagtg gaagggtggg gaagggaaca agggtcttga 3180 gagaaaagaa aggattcgga caatgaacga tgcaagagca ccattgacgt cgggaaggct 3240 gagcgtataa tccaggacga caacgttagt gcaatcgttt gcacaagagg aggttgatgc 3300 acgtgaagtg gacgggtgcc gcgagccttg cgacgaccat cgtcatgtct gcagcgcttc 3360 cgttcgcggc gtttgaaaac acccgttccg tcggcacgga acgcgttctg gcggcgaccg 3420 cggcggatag tactaacgcc tcgcccccga ccggggcgtc cgcaggcggt ggcacagcag 3480 gagagaccta ctcgaatgcc gtgcagattg tgagcggcaa tggctttcgc gctggaaacg 3540 gggccacggt cgtcctcgct gtgaatggac tgaacttgaa cccctcgagt ttcaaggttg 3600 tggtatcgaa tagcctcaac ggcgtgatcg acgtcacaag tgattccgtg ctaacggggc 3660 cgaattcgat tgccttgcac cttcctgccg gggatgacgg gcttggcgcc ggtacgtaca 3720 cggtcaccgt cgaaagtgga ggtgattcgg tctcgacgcc ttcaggacag ggacttcagg 3780 tcatgcctta tacgacggcc gacaccattc agtgggatgg aatctacaca tccgatggcg 3840 cgatgtacgt gtcagatccg aatccctccc ctggacaaga ggtgacgatc agccttcgag 3900 cgtacagcgg gaacctgacg aaggtgatcc tgaattgctg ggacaccgca caaaacaagg 3960 gtttccaggt cgagatgtct ccaggcagaa cgtttggacc gtatcagctc tggtctgcca 4020 cgatccccgc ttcaaacggc ggaacgatct attatcgctt tgacatatac gatggcacca 4080 gttttgcgtg tctctcaggt gacggactgc acacgtctga cgacatcaac aacaatttcc 4140 cgttgcccgt ggggacggtc acgctttcga cacttcaggc gaatcccggt gatacggtga 4200 cggtctccga ccctgtaggt gacttcgccg gaagccagga tcaacccaat cacacggtga 4260 tacggtttgt caactcgtcg ggcgaaacgg ccgccacagt caatgggacg aacgcgagct 4320 ggaacagcgt gcagttcaca gtcccacaga gccttccaaa cggcttgtat cgcgtcgaga 4380 tcgacacggt cgccaaggac gcggatgggg tggtcaatgt cgaattggac aggagtgcgg 4440 agcttattgt agggcctctg cccgcgtgga tgcaagcgta tgcacatgat tcgtttcagg 4500 cgttctaccg atcgcctttc ggagccgtgt ccacaggaac ccccatcacg cttcgcctgc 4560 gggctccgct cagcgtgaag agtgcgacgc ttcgcctctg gggggcagcg gatcagtcag 4620 gcgagatcga cctgccgatg cagaagctcc aaatgtcggg agacgagttg gcgcaacaaa 4680 ccggcgtgca ggacatcaac gactacacgt ggtggacggt gaccatccct gcggcggatg 4740 tgaccacacc cgggacgatg tggtatcagt tcgtgacgga gacggacact ggccaggtgg 4800 tctactacga tgacaatgga gctcagcttg aagggcctgg ccaggttggg ttgtcttccg 4860 acggaccgag ctaccagatc agcgtatacg aacggggatt tcagacgcca gattggctga 4920 agcacgccgt gatctacgaa atcatgccgg atcggttcta caatggcaat atcgccacgg 4980 aggagaatcc gaatacgcaa aaggggattt atgtaggggc cgatggaacg gagtcattag 5040 gccccatcca gttccacgag aactgggact cgccgcccta tgatccgaat attcctccgt 5100 tatctgatcc caaaattgcc agtctgcgag gcaatggcca atggaacatt gactttttcg 5160 gaggtgattt gcagggcatc gaggataagc tggactacct gaagagcctt ggagtcaata 5220 cgctgtatct gatgcccgtc tttgaggcgg aatccaatca caaatatgac acagccgact 5280 atttcaagat tgaccctgga tttggaacgc agcaggactg gctgaatctc gtacaggctg 5340 cgcacgcgaa ggggttccat atcattctcg acggggtgtt cgaagatacc ggaagtgaca 5400 gcgtatattt caacaagttc gggaacttcc actccaacgg tgcgtggcag gcgtacctga 5460 agaaccagcc gtcgctgtcg ccctactact cgtggtacgt gtggacaggg aacacctcaa 5520 acccatacga ttcgtggttt cagatcgaca cgctgccact tacggacacg tcgaaccccg 5580 cctatcagcg attcgtgtat gggagcgaca actcagtcgc gcgtgtgtgg atccgggaag 5640 gtgcggacgg atggcgcttg gactcggccg acaacgggaa tttcaacacg gcatggtggg 5700 gtggctttcg gcaggccgtg aaatcgatcg atcccaacgc agcgatcatc ggcgagatct 5760 gggacaatgc gacgaatgac aatggaacgg attggttgac gggatcgacc ttcgacagtg 5820 taatgaacta ccagttccgg aacgccgtga tcgacttctt ccgcggcacg tacaacgacg 5880 gaaacgtgca gcaccacgcc gtcgacgctg cgggattcaa ccaggaactg atgcgcctgt 5940 acagtgaata tcctctgcag tcgttctact cgatgatgaa ccttgtcgat tcgcaagaca 6000 ccatgcggat cctgaccatc ttggagaacg cgccgcagcc aggcgatcta tccgcgctcc 6060 agcaggatga gtacaggccg tctcctgcgg ctgaacagtt ggggatcgag aggctgaagc 6120 ttgtatcgga ctttcaattc agcttcccgg gcgatccgac catcttctac ggcgacgagg 6180 cagggctcac tggttattcg gatcccctca atcgtcggac ctatccgtgg gacaaccaga 6240 atctcgatct cctgaaccac taccgcaagc tcggggccat tcgaaacgcc aatcctgtgc 6300 ttcagacggg ggatttcacg ccgttgtacg cacagggcat ggtgtacgca tttgcaagga 6360 ccattcggaa tgggcgagat gtcttcggtg tgccagcgga ggatgccacg gccattgtgg 6420 cgatcaacaa tcagaaccaa gctatcaccg tgaccattcc gacggatggg acggttgcgg 6480 acgggtccac gatgctcgat gaactgaaca accagtggta caaggtgcag aatggtggca 6540 ttacactcac gctgcaatcg tatcaaggtg ccattttggt gacgccgagc gacgcgccga 6600 tggcttatct gcaagaggag gattctcaga acgagattgc gtggacgcct gtgcaaggtg 6660 ccatcggtta tcgcgtctgg agacagaatc cgaatggaca atgggtgccc tttggacctg 6720 tgcttcctgc cacggacttg agtgtcacgg tggaacgcga tgcatatgcg caaacgtttg 6780 ctgtacaagc gctgttttcg gcgtctgatc acgcccagtc tccggtgtcg gcacctaaga 6840 cggtatcgct tgccgtcgat gtgcccgcgg tacgcctgag tcagccgatc gttagtggtc 6900 gtgtggttgg agatcgtgcg atggtctcga tcacgccggt ttcaggcgcg acgcagtatg 6960 tgatctacca gagacagggc gacggatcgt atgctccggt cgcgacggtc tccacaagtg 7020 gcgattccgc agctataggg gaagttcctg cgcaaggtcc ggccaactcg cctcacgcga 7080 cgattcgcgt gacagtgccc gtacctgcag gtttctcgtc ggtgacctac cgcgtggctg 7140 cgcaaaacga agatgggcaa gctgtgacca atccattgac cctatcgctc tcgaaaaagt 7200 gatgcctcgc gaaaagggct atcggaattt tttcgagata acatcttgat tgcgatcgga 7260 tgaaagcgtt atactaatcg cgcaaacgtt tgcgcaagca aatgcacaga gggggttgtg 7320 tcggtgagga gatggggaat cgtatcgaca ggtgtggccg cgctcgtgtt ggcgggcgga 7380 cagtcgcggg ctgtggaacc tcgaacggtg gtcaaaacac gtctccttcc acgagctcgt 7440 cgagcgccaa aggggaggca agcgctctgc ccaaaggcga gaccatcacc gtttggtcgt 7500 ggcagacagg gccggagttg caagacgtga agcagatcgc cgctcagtgg gcaaaggctc 7560 atggggacaa ggtcattgtc gtcgaccaaa gctccaatcc gaagggattc cagttctacg 7620 ccacggcggc tcgcacgggc aaggggcctg atgtcgtgtt tggaatgccg cacgacaaca 7680 atggcgtttt cgcagaagaa ggattgatgg cgccggtacc gtctggcgtg ctcaacacgg 7740 gtctctatgc acctaatacg atcgacgcca tcaaggtcaa cggcacgatg tactcggtgc 7800 cagtatccgt tcaggtcgcg gcaatctact acaacaaaaa gcttgtgccg cagccaccgc 7860 agacatgggc cg 7872 // |
|