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EBI DbfetchID X62115; SV 1; linear; genomic DNA; STD; PRO; 2376 BP. XX AC X62115; S75769; XX DT 14-OCT-1991 (Rel. 29, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 8) XX DE K.pneumoniae plasmid pBWH77 aphA7 and blaS2A genes for neomycin DE phosphotransferase and beta-lactamase XX KW aphA7 gene; beta-lactamase; blaS2a gene; neomycin phosphotransferase; KW R plasmid. XX OS Klebsiella pneumoniae OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Klebsiella. OG Plasmid pBWH77 XX RN [1] RP 1-2376 RA Syvanen M.; RT ; RL Submitted (30-AUG-1991) to the EMBL/GenBank/DDBJ databases. RL M. Syvanen, School of Medicine, UC-Davis, Davis California 95616, USA XX RN [2] RP 1-413, 1223-2376 RA Lee K.Y., Hopkins J.D., Syvanen M.; RT "Evolved neomycin phosphotransferase from an isolate of Klebsiella RT pneumoniae."; RL Mol. Biol. 5:0-0(1991). XX RN [3] RP 1-2376 RX PUBMED; 2160941. RA Lee K.Y., Hopkins J.D., Syvanen M.; RT "Direct involvement of IS26 in an antibiotic resistance operon"; RL J. Bacteriol. 172(6):3229-3236(1990). XX RN [4] RX DOI; 10.1111/j.1365-2958.1991.tb00826.x RX PUBMED; 1662755. RA Lee K.Y., Hopkins J.D., Syvanen M.; RT "Evolved neomycin phosphotransferase from an isolate of Klebsiella RT pneumoniae"; RL Mol. Microbiol. 5(8):2039-2046(1991). XX CC R-Plasmid, overlap of sequenced fragments XX FH Key Location/Qualifiers FH FT source 1..2376 FT /organism="Klebsiella pneumoniae" FT /plasmid="pBWH77" FT /strain="5214773" FT /mol_type="genomic DNA" FT /db_xref="taxon:573" FT repeat_region 1..343 FT /mobile_element="insertion sequence:IS26" FT -35_signal 330..336 FT -10_signal 354..360 FT CDS 410..1225 FT /transl_table=11 FT /gene="aphA7" FT /product="neomycin phosphotransferase" FT /db_xref="GOA:Q79AN4" FT /db_xref="InterPro:IPR008266" FT /db_xref="UniProtKB/TrEMBL:Q79AN4" FT /protein_id="CAA44024.1" FT /translation="MSHIQRETSCSRPRLNSNLDADLYGYRWARDNVGQSGATIYRLYG FT KPNAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTAIPGK FT TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA FT SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI FT ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" FT CDS 1281..2141 FT /transl_table=11 FT /gene="blaS2A" FT /product="SHV2A beta-lactamase" FT /db_xref="GOA:P0A9Z8" FT /db_xref="HSSP:1N9B" FT /db_xref="InterPro:IPR000871" FT /db_xref="UniProtKB/Swiss-Prot:P0A9Z8" FT /protein_id="CAA44025.1" FT /translation="MRYIRLCIISLLATLPLAVHASPQPLEQIKLSESQLSGRVGMIEM FT DLASGRTLTAWRADERFPMMSTFKVVLCGAVLARVDAGDEQLERKIHYRQQDLVDYSPV FT SEKHLADGMTVGELCAAAITMSDNSAANLLLATVGGPAGLTAFLRQIGDNVTRLDRWET FT ELNEALPGDARDTTTPASMAATLRKLLTSQRLSARSQRQLLQWMVDDRVAGPLIRSVLP FT AGWFIADKTGASERGARGIVALLGPNNKAERIVVIYLRDTPASMAERNQQIAGIGAALI FT EHWQR" XX SQ Sequence 2376 BP; 537 A; 628 C; 649 G; 562 T; 0 other; cccggtacag atacgcccag cggccattga ccttcacgta ggtttcatcc atgtgccacg 60 ggcaaagatc ggaagggtta cgccagtacc agcgcagccg tttttccatt tcaggcgcat 120 aacgctgaac ccagcggtaa atcgtggagt gatcgacatt cactccgcgt tcagccagca 180 tctcctgcag ctcacggtaa ctgatgccgt atttgcagta ccagcgtacg gcccacagaa 240 tgatgtcacg ctgaaaatgc cggcctttga atgggttcat gtgcagctcc atcagcaaaa 300 ggggatgata agtttatcac caccgactat ttgcaacagt gccgataaaa atatatcatc 360 atgaacaata aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat 420 tcaacgggaa acgtcttgct cgaggccgcg attaaattcc aacctggatg ctgatttata 480 tgggtataga tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgattgta 540 tgggaagccc aatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga 600 tgttacagat gagatggtca gactaaactg gctgacggca tttatgcctc ttccgaccat 660 caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccgggaa 720 aacagcattc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct 780 ggcagtgttc ctgcgccggt tgcattcgat tcctgtttgt aattgtcctt ttaacagcga 840 tcgcgtattt cgtctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgctag 900 tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa 960 gcttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct 1020 tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga 1080 ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca 1140 gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca 1200 tttgatgctc gatgagtttt tctgatatgt cgcttcttta ctcgccttta tcggccctca 1260 ctcaaggatg tattgtggtt atgcgttata ttcgcctgtg tattatctcc ctgttagcca 1320 ccctgccgct ggcggtacac gccagcccgc agccgcttga gcaaattaaa ctaagcgaaa 1380 gccagctgtc gggccgcgta ggcatgatag aaatggatct ggccagcggc cgcacgctga 1440 ccgcctggcg cgccgatgaa cgctttccca tgatgagcac ctttaaagta gtgctctgcg 1500 gcgcagtgct ggcgcgggtg gatgccggtg acgaacagct ggagcgaaag atccactatc 1560 gccagcagga tctggtggac tactcgccgg tcagcgaaaa acaccttgcc gacggcatga 1620 cggtcggcga actctgcgcc gccgccatta ccatgagcga taacagcgcc gccaatctgc 1680 tactggccac cgtcggcggc cccgcaggat tgactgcctt tttgcgccag atcggcgaca 1740 acgtcacccg ccttgaccgc tgggaaacgg aactgaatga ggcgcttccc ggcgacgccc 1800 gcgacaccac taccccggcc agcatggcgg cgaccctgcg caagctgctg accagccagc 1860 gtctgagcgc ccgttcgcaa cggcagctgc tgcagtggat ggtggacgat cgggtcgccg 1920 gaccgttgat ccgctccgtg ctgccggcgg gctggtttat cgccgataag accggagcta 1980 gcgagcgggg tgcgcgcggg attgtcgccc tgcttggccc gaataacaaa gcagagcgca 2040 ttgtggtgat ttatctgcgg gataccccgg cgagcatggc cgagcgaaat cagcaaatcg 2100 ccgggatcgg cgcggcgctg atcgagcact ggcaacgcta acccggcggt ggccgcgcgc 2160 gttatccggc ccgcagcacc tcgcaggcgt gccggtgcga tatgactggc gtgcggcatc 2220 ggaaaagccg cgtgcggtaa ttgatgctgg tgaacctggg tcaaaggtaa cgcccataaa 2280 cggtggccga cctgacttgt agtttcgaag cctgtcgcac tgccaggcat gcttgtggcg 2340 ctgacctgct gaccgctcct tgacctggta ccttct 2376 // |
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