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EBI DbfetchID X62073; SV 1; linear; genomic DNA; STD; PRO; 4454 BP. XX AC X62073; S76412; XX DT 04-MAR-1993 (Rel. 35, Created) DT 30-JUN-2006 (Rel. 88, Last updated, Version 9) XX DE Erwinia chrysanthemi kdgF, kduI, kduD, pelW genes XX KW 2-keto-3-deoxygluconate oxydoreductase; 5-keto-4-deoxyuronate isomerase; KW exopolygalacturonate lyase; kdgF gene; kduD gene; kduI gene; KW pecin metabolism; pectin degradation; pelW gene. XX OS Erwinia chrysanthemi OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Dickeya. XX RN [1] RX DOI; 10.1111/j.1365-2958.1991.tb02149.x RX PUBMED; 1766386. RA Condemine G., Robert-Baudouy J.; RT "Analysis of an Erwinia chrysanthemi gene cluster involved in pectin RT degradation"; RL Mol. Microbiol. 5(9):2191-2202(1991). XX RN [2] RC Revised by [3] RA Condemine G.; RT ; RL Submitted (24-MAR-1992) to the EMBL/GenBank/DDBJ databases. RL G. Condemine, Laboratoire de Genetique Moleculaire des Microorganismes, I N RL S A Batiment 406, 20 Av Albert Einstein, 69621 Villeurbanne, FRANCE XX RN [3] RP 1-4454 RA Hugouvieux-Cotte-Pattat N.; RT ; RL Submitted (12-JAN-1999) to the EMBL/GenBank/DDBJ databases. RL N. Hugouvieux-Cotte-Pattat, Laboratoire de Genetique Moleculaire des RL Microorganismes, I N S A Batiment 406, 20 Av Albert Einstein, 69621 RL Villeurbanne, FRANCE XX RN [4] RX PUBMED; 10383957. RA Shevchik V.E., Condemine G., Robert-Baudouy J., Hugouvieux-Cotte-Pattat N.; RT "The exopolygalacturonate lyase PelW and the oligogalacturonate lyase Ogl, RT two cytoplasmic enzymes of pectin catabolism in Erwinia chrysanthemi 3937"; RL J. Bacteriol. 181(13):3912-3919(1999). XX FH Key Location/Qualifiers FH FT source 1..4454 FT /organism="Erwinia chrysanthemi" FT /strain="3937" FT /mol_type="genomic DNA" FT /db_xref="taxon:556" FT CDS complement(83..415) FT /transl_table=11 FT /gene="kdgF" FT /db_xref="InterPro:IPR014710" FT /db_xref="UniProtKB/Swiss-Prot:Q05527" FT /protein_id="CAA43987.1" FT /translation="MRRYFIDDETPWEELGDGIKRKIITWSDDLMMVCVHFAKGAIGTP FT HKHDIHDQIAYVAAGSFEVVIEGEKRILKTGDAYMAVKNEMHGVVSLEEGSVLIDTFSP FT KRADFL" FT RBS complement(420..426) FT misc_feature 548..574 FT /note="KdgR box" FT RBS 727..732 FT CDS 738..1574 FT /transl_table=11 FT /gene="kduI" FT /product="5-keto-4-deoxyuronate isomerase" FT /db_xref="GOA:Q05529" FT /db_xref="InterPro:IPR007045" FT /db_xref="UniProtKB/Swiss-Prot:Q05529" FT /protein_id="CAA43988.1" FT /translation="MQVRQSIHSDHARQLDTAGLRREFLIEHIFDADACTMTYSHIDRI FT IVGGVMPVHQAVTVGEDDGKQLGVTYFLERRELGAINIGGAGVVSVDGERYAIGHEEAI FT YIGKGARDIRFTSVDPAKPARFYYNSAPAHTTFPTRKITAAEASPQTIGDDATSNRRTI FT NKYIVPDVLPTCQLTMGLTKLAEGNLWNTMPCHTHERRMEVYFYFDMDEETAVFHMMGQ FT PQETRHILVHNEQAVISPSWSIHSGVGTKRYTFIWGMVGENQVFSDMDHVKVSELR" FT misc_feature 1682..1709 FT /note="KdgR box" FT RBS 1741..1747 FT CDS 1756..2517 FT /transl_table=11 FT /gene="kduD" FT /product="2-keto-3-deoxygluconate oxydoreductase" FT /db_xref="GOA:Q05528" FT /db_xref="HSSP:1EDO" FT /db_xref="InterPro:IPR016040" FT /db_xref="UniProtKB/Swiss-Prot:Q05528" FT /protein_id="CAA43989.1" FT /translation="MILNTFNLQGKVALITGCDTGLGQGMAVGLAEAGCDIVGVNIVEP FT KETIEKVTAVGRRFLSLTADMSDISGHAALVEKAVAEFGKVDILVNNAGIIRREDAIEF FT SEKNWDDVMNLNIKSVFFMSQTVARQFIKQGHGGKIINIASMLSFQGGIPVPSYTASKK FT RVMGITRIVANEWAKHNINVNAIAPGYMATNNTQHVRADQDRSKEILDRIPAGRWGLPQ FT DLQGPAVFLASSASDYVNGYTIAVDGGWLAR" FT misc_feature 2713..2739 FT /note="KdgR box" FT RBS 2766..2771 FT CDS 2778..4439 FT /transl_table=11 FT /gene="pelW" FT /product="exopolygalacturonate lyase" FT /EC_number="4.2.2.9" FT /db_xref="GOA:Q05526" FT /db_xref="InterPro:IPR010702" FT /db_xref="UniProtKB/Swiss-Prot:Q05526" FT /protein_id="CAA43990.1" FT /translation="MSIFTDLNTSRKWQIDQWLSAVNSHIEKIQQYGHSVVNPTPLLAD FT GFEIKTQSPVVWQFPDGHDAPISNFASQQNWLRLLISMSVITETEKYRHLAFCQSEYFL FT NRFVDENSGLFYWGGHRFINLDTLASEGPESKSMVHELKHHLPYYEFLHQVNPEKTRHF FT IQGFWNAHVEDWSCLDLGRHGDYARQRDPDVFLHSRHDVVTPANWPELPLTKGLTFVNA FT GTDLIYAAFVYARHTGDAHAAAWGKHLYRQYVLARNPETGMPVYQFSSPLQRQPVPADD FT NQTQSWFGDRAQRQFGPEFGAIAREANVLFRDMRPLLIDNPLAMLDILRHQPDAEILTW FT VIAGLKNYYQYAYDVNSNSLRPMWNNGQDMTDYCFKRDGYYGKAGTVLKPFPLEGDYLL FT PLVRAWLLSDDDDLHTLIVTMLSRLEKQGIHQSASPFLLLAITELAHAKQSAQWAEYAW FT QMAEILFKRYFHHGLFVRSEHHRYVRLDDPFPAILLTLIAACRNKWSEVPAVLTQGGYI FT HGDYRINGESRVIYDTGIYLPRKINPLILFLQIHHY" XX SQ Sequence 4454 BP; 1137 A; 1190 C; 1090 G; 1037 T; 0 other; cagaccgcat aagcggacta taaaggaaag aattgaaaga aaggatttcg acaaggtgcc 60 ggcaccttat taatagaagg aatcagagga agtcagcacg tttcggcgag aaggtgtcga 120 tcaacacgct gccttcttcc agcgatacta caccgtgcat ttcattcttc accgccatat 180 aggcatcgcc ggttttcaga atgcgttttt cgccttcgat caccacttcg aagctaccgg 240 ccgccacata ggcgatctga tcgtggatat cgtgcttgtg cggcgtaccg atggcaccct 300 tggcgaaatg aacgcacacc atcatcaaat catcgctcca ggtgatgatt ttacgcttaa 360 tgccatcgcc cagctcttcc cacggcgttt catcgtcaat aaagtacctt ctcatggttt 420 ctccttaaga tcatataacg tgcctccctt gtgtgaacaa ggtaacacaa agggtagata 480 ccgatattct agtaacatcg gcattagatg aaacataaaa taagcaaaac catgacgcct 540 ctcaagaaat aaataaaaca ttatttcatt tttattgaat tcacatctca accacactat 600 catcgcgcca taacacgaaa gaaccgggca aaaaaggttc aggtgacgtt gtcactgcca 660 cgcaaggtga cctgttccgc ccggcgcttt cctgtcaggg tcacagcctg atttctatct 720 gaaagcgagg agcaaccatg caagtccgtc agagcatcca tagcgaccac gcccgtcagc 780 ttgataccgc tggcctgcgg cgcgagtttc tgatcgaaca catttttgat gcagacgcct 840 gcaccatgac ctatagccac atcgatcgaa tcatcgtggg cggggtaatg cccgttcatc 900 aggcggtgac tgtcggcgag gatgatggaa aacagctggg cgtcacgtac tttcttgaac 960 gccgcgaact gggcgcaatc aatatcggcg gcgccggcgt agtcagtgta gacggcgaac 1020 gctatgcaat cggtcacgaa gaagccattt atatcggcaa aggcgcgcgg gatatccgtt 1080 ttaccagcgt agacccggcc aaacctgccc gtttctacta taacagcgcg ccggcgcaca 1140 ccacctttcc gacacgcaag attaccgctg cagaagcctc cccgcagaca atcggtgatg 1200 acgcgaccag caatcgtcgg accatcaaca agtatatcgt tccggatgtg ttgcccacct 1260 gccagctcac catggggtta accaagctgg cggaaggcaa tctctggaac accatgccgt 1320 gccataccca tgagcgccgc atggaagtct atttttattt cgatatggat gaagaaacgg 1380 cggtgttcca catgatggga cagccacagg aaacccgcca cattctggtg cacaacgagc 1440 aggcggtgat ttcaccgagc tggtcgattc attcaggtgt aggcaccaaa cgctacacct 1500 ttatctgggg tatggttggc gagaatcagg ttttctcaga catggatcac gtcaaagtta 1560 gcgaactacg ctagcggctt acccaataat ttcgcgttgc gggaaagtgg caaatgagcg 1620 cgtccccggt cagtcatcgg gatgagtgac agcagccagc aagccggcaa cctgaaatat 1680 gacgggtata aaccggtatt tccctacagt aacaagctca cgacaaaaca tgtcgtttac 1740 agagagatta aagctatgat tctgaataca ttcaatttac agggcaaagt ggcactgatc 1800 accggttgcg ataccggcct gggtcagggt atggcggtcg gtctggctga agcgggctgc 1860 gacatcgtcg gcgtcaacat cgttgagccg aaagaaacca tcgaaaaagt cactgcggta 1920 ggccgtcgct tcctcagcct gaccgctgat atgagcgata tctccggtca cgccgcgctg 1980 gtggaaaaag ccgttgccga gttcggtaaa gtggacattc tggtcaacaa cgccggtatc 2040 atccgtcgtg aagacgccat cgagttcagc gaaaagaact gggacgatgt gatgaacctg 2100 aacatcaaga gtgtgttctt catgtcgcag acggttgctc gtcagttcat caagcagggt 2160 catggcggta aaattattaa tatcgcgtcc atgttgtcct tccagggcgg tatcccggta 2220 ccgtcttaca ccgcatccaa gaagcgcgtc atgggcatca cccgtattgt ggccaatgaa 2280 tgggcgaaac ataacatcaa cgtcaacgcc atcgctcccg gctacatggc gaccaacaac 2340 acccagcacg tgcgcgccga tcaggaccgt agcaaggaaa ttctggaccg tatcccagcg 2400 ggtcgttggg gtctgccgca ggacttgcag ggtccggccg tgttcctggc atccagcgca 2460 tccgattatg tgaacggtta caccattgcc gttgatggcg gctggttggc tcgctaattt 2520 tccgcaaaat ttttttgtga tcgaaggcac aatactgaat tgtgcctttc ttttatctat 2580 caataattta tcatcaaccc gcctttcctc ttcgtttcca aatcttccat aaaaaatttt 2640 caaaacaacg ttccgacttt gatcacactt tcgatattga gcgcatgacg aaaatagaag 2700 ttgggcaata taatcaatca aaacaatgtt tctatttata aggaactgtt tcgctcagtt 2760 ctgaaagaag gtactccatg agtattttta ctgatttgaa caccagcaga aaatggcaaa 2820 tcgaccagtg gctctcggcg gttaacagcc atattgaaaa gattcagcaa tacggccaca 2880 gcgtagtcaa cccaacgccg ttattggctg acggatttga gataaaaaca cagtctccag 2940 ttgtctggca gtttcccgat ggtcatgatg ctccgatttc taacttcgcc agccagcaaa 3000 actggctgcg gttattaatt tccatgagcg tcatcacaga aacggaaaaa tatcgtcatc 3060 tggcattctg tcagagcgaa tattttctga atcgctttgt cgatgaaaat agcgggcttt 3120 tttattgggg cggccatcgt tttattaatc tcgatacgct ggcaagcgaa ggcccggaat 3180 ccaaatcaat ggtgcatgaa ttaaaacacc atctgcccta ttacgaattt ctgcatcagg 3240 tcaacccgga aaaaacccgc catttcattc agggattctg gaatgctcac gtcgaagact 3300 ggagctgcct ggatctgggc cgccatggtg attacgccag acaacgcgat ccggacgtgt 3360 ttctgcattc ccgccatgat gtggtcaccc ctgccaactg gccggagtta ccgctgacca 3420 aagggctgac cttcgtcaat gccggcaccg acctgatcta tgccgccttt gtctacgcac 3480 gccacaccgg cgacgcgcac gcggcggcct ggggcaagca cctctatcgt caatatgtgc 3540 tggcgcgtaa cccggaaacc ggtatgccgg tttaccagtt tagctctcct ttgcaacgcc 3600 agccggtgcc ggcggacgac aaccagaccc aatcctggtt tggcgaccgc gcccagcggc 3660 aatttgggcc ggagtttggc gccattgccc gtgaagccaa cgtgctgttt cgcgacatgc 3720 gcccgctgct gatcgataac ccactggcca tgctcgatat tctgcgccat caacccgatg 3780 cggaaatcct gacatgggtg atcgccggcc tgaaaaacta ctatcaatac gcttacgacg 3840 ttaacagcaa tagcctgcgc ccgatgtgga ataacggtca ggacatgacc gactactgct 3900 tcaaacgcga cggctactac ggcaaagccg gcaccgtact gaagcccttc ccgctggaag 3960 gcgactacct gctgccgctg gtacgtgcct ggctcctgag cgacgatgac gacctgcaca 4020 cactgatcgt caccatgctg tcccgactgg agaaacaggg catccaccaa tccgcttcgc 4080 cgttcctgct gctggcgatt accgaactgg cgcatgccaa acagtcggcg cagtgggcgg 4140 aatatgcctg gcaaatggcg gaaattttat ttaagcgcta tttccatcac ggtttattcg 4200 tacgttctga acatcatcgt tatgtgcggc ttgatgatcc cttcccagcc attctgctga 4260 cgctgatcgc tgcctgcaga aataaatggt cggaagttcc tgcggtcctg acacaagggg 4320 gatacatcca tggagattat cgaattaatg gagaaagccg ggttatttac gacacgggaa 4380 tttatttacc cagaaaaatt aatccactga ttttattttt acagattcac cattactaaa 4440 agtaggtagg catt 4454 // |
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