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EBI DbfetchID X61207; SV 1; linear; genomic DNA; STD; PRO; 4746 BP. XX AC X61207; XX DT 23-SEP-1991 (Rel. 29, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 7) XX DE A.brasilense hisB, H, A, F and E genes for imidazole glycerolphosphate DE dehydratase, glutamine amidotransferase, DE phosphorybosilformimino-5-amino-phosphorybosil-4-imidazolecarboxamide DE isomerase, cyclase and phosphorybosil-AMP-cyclohydrolase XX KW cyclase; glutamine amido transferase; hisA gene; hisB gene; hisE gene; KW hisF gene; hisH gene; histidine operon; KW imidazoleglycerol-phosphate dehydratase; phosphorybosil-AMP-cyclohydrolase; KW phosphorybosilformimino-5-amino-phosphorybosil-4-imidazolecarboxamide isomerase. XX OS Azospirillum brasilense OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; OC Rhodospirillaceae; Azospirillum. XX RN [1] RP 1-4746 RA Fani R.; RT ; RL Submitted (09-AUG-1991) to the EMBL/GenBank/DDBJ databases. RL R. Fani, Dipartimento di Biol Animale E Genetica, Univ di Firenze, Via RL Romana 17, 50125 Firenze, ITALY XX RN [2] RP 1-4746 RX DOI; 10.1007/BF00334360 RX PUBMED; 2664449. RA Fani R., Allotta G., Bazzicalupo M., Gallori E., Polsinelli M.; RT "Cloning of the histidine biosynthetic genes of Azospirillum brasilense: RT organisation, nucleotide sequence and analysis of the hisH, hisA, hisF and RT hisE genes"; RL Mol. Gen. Genet. 216(2-3):224-229(1989). XX RN [3] RX DOI; 10.1016/0923-2508(93)90044-3 RX PUBMED; 8210676. RA Fani R., Alifano P., Allotta G., Bazzicalupo M., Carlomagno M.S., RA Gallori E., Rivellini F., Polsinell M.; RT "The histidine operon of Azospirillum brasilense: organization, nucleotide RT sequence and functional analysis"; RL Res. Microbiol. 144(3):187-200(1993). XX FH Key Location/Qualifiers FH FT source 1..4746 FT /organism="Azospirillum brasilense" FT /strain="Sp6" FT /mol_type="genomic DNA" FT /db_xref="taxon:192" FT CDS 404..1027 FT /transl_table=11 FT /gene="hisB" FT /product="Imidazole glycerolphosphate dehydratase" FT /db_xref="GOA:P18787" FT /db_xref="InterPro:IPR000807" FT /db_xref="UniProtKB/Swiss-Prot:P18787" FT /protein_id="CAA43515.1" FT /translation="MDQSLANGVGGASIERNTTETRIRVAVNLDGTGVYDVKTGVGFLD FT HMLEQLSRHSLMDLSVAAEGDVHIDAHHTTEHSGIAIGQAVAKAVGDRKGIQRYGHAYV FT PMDETLTRVALDFSNRPYLIWKVSFSRDKIGDMDTELFREWFQAFAMAAGVTLHVECLY FT GENNHHIVESCYKALARALRAGIEIDPRKRDAVPSTKGTLGGSL" FT CDS 1080..1658 FT /transl_table=11 FT /gene="hisH" FT /product="glutamine amidotransferase" FT /db_xref="GOA:P18785" FT /db_xref="HSSP:1GPW" FT /db_xref="InterPro:IPR016226" FT /db_xref="UniProtKB/Swiss-Prot:P18785" FT /protein_id="CAA43516.1" FT /translation="METVALIDYGSGNLRSPAKALERAAAGCHASFQVLVTSDADAVRK FT ADRVVLPAVGAFADCKRGLSEVPGMQLMAEGGREYGVTEGLGWIKGEVVKLEPADPTLK FT IPHMGWNELDIRREHPVLAGLRERAHAYFVHSYRFAVERPEDVIASADYGGPFAAVVGR FT DNLVGTQFHPEKSQETGLALVANFLTWRV" FT CDS 1661..2167 FT /transl_table=11 FT /gene="ORF1" FT /function="Unknown" FT /db_xref="GOA:P26723" FT /db_xref="InterPro:IPR000182" FT /db_xref="UniProtKB/Swiss-Prot:P26723" FT /protein_id="CAA43517.1" FT /translation="MTDVTVERIDTLKTGDLHDLCDAADDAVKAGGGFGWVAPPAREIM FT ERYWKGVLVVPERILFVGRLDGVIAGSAQLVKPARNNEAQAHAATLTTSFVAPWARCHG FT LARRLTVAVEEEARASGFRVLNLDVRETQRRQPSRCTRIWASAVGARIPSMRWWTAKGW FT PDISM" FT CDS 2220..2984 FT /transl_table=11 FT /gene="hisA" FT /product="phosphorybosilformimino-5-amino- FT phosphorybosil-4-imidazolecarboxamide isomerase" FT /db_xref="GOA:P26720" FT /db_xref="InterPro:IPR006062" FT /db_xref="UniProtKB/Swiss-Prot:P26720" FT /protein_id="CAA43518.1" FT /translation="MIIYPAIDLKDGACVRLLRGEMSQATVFNTEPADQARLFESQGFE FT WLHLVDLNGAFEGKPVNGKAVESILGAVTVPVQLGGGIRDLKTIALWLEKGVSRVILGT FT VALREPELVREARREFPGKVAVGIDAREGYVAVAGWAETSTIKALDLALKFEDSGVAAI FT IYTDINRDGAMGGVNVEATSDLAFHLTTPVIASGAVSSIDDLIALKKEEDTGIQGVICG FT RALYDGRIDPKTALDLLSSVSVSGTPVTGKAG" FT CDS 2984..3769 FT /transl_table=11 FT /gene="hisF" FT /product="cyclase" FT /db_xref="GOA:P26721" FT /db_xref="HSSP:1GPW" FT /db_xref="InterPro:IPR004651" FT /db_xref="UniProtKB/Swiss-Prot:P26721" FT /protein_id="CAA43519.1" FT /translation="MLKMRVIPCLDVKDGRVVKGVNFVDLIDAGDPVEQARVYDREGAD FT ELTFLDITASHENRDTIYDVVRRTAEQVFMPLTVGGGVRTVDDIRKLLLAGADKVSINT FT AAIHRPEFVQEAAEKFGAQCIVVAIDAKQVEPGRWEIFTHGGRKATGIDAIEWAKRMES FT YGAGEILLTSMDRDGTKSGFDLALTRKVADGLRIPVIASGGVGTLDHLVEGIREGHATA FT VLAASIFHFGTYTIGQAKAALAEAGIPVRPARMAEAAHG" FT CDS 3762..4097 FT /transl_table=11 FT /gene="hisE" FT /product="phosphorybosil-AMP-cyclohydrolase" FT /db_xref="GOA:P26722" FT /db_xref="InterPro:IPR008179" FT /db_xref="UniProtKB/Swiss-Prot:P26722" FT /protein_id="CAA43520.1" FT /translation="MAEDKISAAVLDRLYATVQARKGADPETSYTAKLFHRGTAKIAQK FT VGEEAVETVIEAVRGDKAAMASESADLLYHLMVLWADAGLEPAAVWEKLAQREGTSGIA FT EKNARKS" FT CDS 4129..4497 FT /transl_table=11 FT /gene="ORF2" FT /function="Unknown" FT /db_xref="GOA:P26724" FT /db_xref="HSSP:1KPF" FT /db_xref="InterPro:IPR001310" FT /db_xref="UniProtKB/Swiss-Prot:P26724" FT /protein_id="CAA43521.1" FT /translation="MAKTYDPNNVFARILRGEIPCKKVLETEHALAFHDINPQAPTHIL FT VIPKGAYVDMDDFSARATEAEIAGLFRAVGEVARGAGAAEPGYRILSNCGEDANQEVPH FT LHIHVFAGRRLGPMITKG" FT terminator 4542..4576 XX SQ Sequence 4746 BP; 830 A; 1628 C; 1597 G; 691 T; 0 other; gaattctcgt gcagactgcg cccgctggtc caatagacgt cctcataggg cggccagcac 60 tccagcgccc agacatagcg ccccgtcacc atgccccgcc gcatcgagcc gagcgagagg 120 ccgtcgaagc ggaccggtcc cgtcgaaccg cgggcgacgg gtggggattt gcgggtctgc 180 ggcagctccc cgcccatgca ggcggccagc accagcaggg ccgcgaggga caggaaaagg 240 cgtccgggcg tcggcatgtc cgatgccata tcccattatg cgcgttgagc gtgcacacga 300 ccatgcacac acacgcatta tcgggcatcg cgaccgacgg tcataggcgt tttcggaggc 360 ggcacctctg ccgcccgcgc gcttcctgat aaaacccgga ctcatggacc agagccttgc 420 caatggcgtc ggcggcgcct cgatcgagcg gaacaccacc gaaacccgga tccgggtcgc 480 cgtgaatctg gacggcaccg gcgtctatga cgtgaagacc ggcgtcggct tcctcgacca 540 catgctggag cagctgtcgc gccacagcct gatggacctg tcggtggcgg cggagggcga 600 cgtgcatatc gacgcccacc acacgaccga gcacagcggc atcgccatcg gccaagcggt 660 ggcgaaggcg gtgggcgacc gcaagggcat ccagcgctac ggccacgcct acgtcccgat 720 ggacgagacg ctgacccgcg tcgcgctcga cttctccaac cggccctacc tgatctggaa 780 ggtgtccttc agccgcgaca agatcggcga catggacact gagctgttcc gcgagtggtt 840 ccaggccttc gccatggccg cgggcgtgac gctgcacgtc gaatgcctct atggcgagaa 900 caaccaccac atcgtggaaa gctgctacaa ggcgcttgcc cgcgcgctgc gggccgggat 960 cgaaatcgac ccgcgcaagc gcgacgccgt gccctcgacc aaagggacgc tgggcggctc 1020 cctctgaaaa aaaccggcgg cttcgacaag gccgggtctc tgctgaaggc tttcgtttca 1080 tggaaaccgt tgcgctgatc gattacggct ccggcaacct gcgttcgccc gccaaggctc 1140 tggagcgcgc ggcggcgggg tgccatgcct ccttccaagt cttggtgacc tccgacgccg 1200 acgccgtgcg caaggccgac cgcgtcgtcc tgccggccgt cggcgccttc gccgactgca 1260 agcgcggcct gtcggaggtt cccggcatgc agctgatggc ggagggcggg cgcgagtacg 1320 gcgtgaccga ggggctgggc tggatcaagg gcgaggtggt gaagctggag cccgccgacc 1380 cgacgctgaa gatcccgcac atgggctgga acgagctgga catccgccgg gagcacccgg 1440 tgctggccgg cctgcgcgag cgggcgcacg cctacttcgt ccactcctac cgcttcgccg 1500 tggagcggcc ggaggacgtg atcgccagcg ccgattacgg cgggcctttc gcagcggtgg 1560 tcggacgcga caatctggtc ggcacccagt tccacccgga aaagagccag gagaccggcc 1620 tcgccctggt cgccaacttc ctgacctggc gggtctgacg atgaccgacg tcacggtcga 1680 gcgcatcgac acgctgaaga ccggcgatct gcacgacctg tgcgacgccg ccgacgacgc 1740 cgtcaaggcg gggggcggct tcggctgggt ggcgccgccc gcccgcgaaa tcatggaacg 1800 ctattggaag ggcgttctgg tggtgccgga acgcatcctg ttcgtcggcc ggctggacgg 1860 cgtcatcgcc ggctccgccc agctggtgaa gccggcccgc aacaacgagg cgcaggccca 1920 cgccgccacc ctgaccacca gcttcgtcgc cccctgggcg cgctgccacg ggctggcccg 1980 ccgcctgacc gtggcggtgg aggaggaggc gcgggcgtcc ggcttccgcg tgctgaacct 2040 cgatgtgcgc gaaacccaga ggcggcagcc atcacgctgt acgaggatct gggcttccgc 2100 cgttggggca cgcatccctt ctatgcgctg gtggacggca aaaggctggc cggacatttc 2160 tatgtgaaag acctcacccc cgacacgctt cacgacacgc cataggacga gacaccgcga 2220 tgatcatcta tcccgccatc gacctcaaag acggtgcctg cgttcggctg ctgcgcggcg 2280 agatgagcca ggcgaccgtc ttcaacaccg aacccgccga ccaggcccgc ctgttcgaaa 2340 gccagggctt cgaatggctg catctggtcg atctgaacgg cgcctttgaa ggcaagccgg 2400 tcaacggcaa ggcggtcgag agcatcctgg gcgccgtgac cgttccggtg cagttgggcg 2460 gcggcatccg cgacctgaag accatcgccc tgtggctgga gaagggcgtc agccgcgtca 2520 tcctgggcac cgtcgccttg cgcgagccgg aactggtccg cgaagcccgc cgggagttcc 2580 ccggcaaggt cgccgtgggc atcgacgcgc gcgagggtta tgtggcggtc gccggctggg 2640 ccgagacctc gaccatcaag gcgctggatc tggcgctgaa gttcgaggac agcggcgtcg 2700 ccgccatcat ctacaccgac atcaaccgcg acggtgccat gggcggcgtg aacgtcgagg 2760 cgacctccga cctcgccttc cacctgacca ccccggtgat cgcgtcgggc gccgtttcct 2820 ccatcgacga tctgatcgcg ttgaagaagg aggaggacac cggcatccag ggcgtcatct 2880 gcggccgcgc gctctacgac gggcgcatcg atccgaagac ggcgcttgat ctcctctcat 2940 ccgtttccgt ttctggcacc cccgtcaccg gaaaggccgg ctgatgctca agatgcgcgt 3000 catcccctgc ctggacgtca aggacgggcg ggtcgtcaag ggggtcaact tcgtcgatct 3060 gatcgacgcc ggcgaccccg tggagcaggc ccgcgtctat gaccgggaag gggcggacga 3120 gctgaccttc ctcgacatca cggccagcca cgagaaccgc gacaccatct acgacgtggt 3180 gcgccgcacc gccgagcagg tgttcatgcc gctgaccgtc ggcggcggcg tgcgcacggt 3240 ggacgacatc cgcaagctgc tgctggccgg cgccgacaag gtgtcgatca acaccgcggc 3300 catccaccgt ccggagttcg tccaagaggc ggcggagaag ttcggcgccc aatgcatcgt 3360 cgtcgccatc gacgccaagc aggtcgagcc aggccgctgg gagatcttca cccacggcgg 3420 gcgcaaggcc acgggcatcg acgccatcga gtgggccaag cgcatggagt cctacggggc 3480 cggcgaaatc ctgctgacct cgatggaccg cgacggcacc aagagcggct tcgacctcgc 3540 cctgacccgc aaggtggccg acgggctgcg cataccggtg atcgcgtcgg gcggcgtcgg 3600 caccctggac catctggtgg agggcatccg tgagggccac gccacggcgg tgctggcggc 3660 ctccatcttc catttcggca cctacaccat cgggcaggcc aaggcggcgc tggccgaggc 3720 cggcattccg gtgcgcccgg cccgcatggc ggaggccgca catggctgag gacaagatct 3780 ccgccgcggt cctggaccgg ctctacgcca ccgtccaggc gcgcaagggg gccgatccgg 3840 agacctccta caccgccaag ctgttccacc gcggcaccgc caagatcgcc cagaaggtcg 3900 gcgaggaagc ggtggagacg gtcatcgagg cggtgcgcgg cgacaaggcg gccatggcct 3960 cggaatcggc ggacctgctc tatcacctga tggtgctgtg ggccgacgcc gggctggagc 4020 ccgccgcggt gtgggagaag ctggcccagc gcgagggcac cagcggcatc gccgagaaga 4080 acgcgcgcaa gtcttaaaga acatcaaggc ggaaggggaa acgacgcgat ggccaagacc 4140 tacgatccga acaacgtctt cgcccgcatc ctgcgcggcg aaatcccgtg caagaaggtc 4200 ctggagaccg agcacgctct ggccttccac gacatcaacc cgcaggcccc gacgcacatc 4260 ctggtgatcc ccaagggcgc ctacgtcgac atggacgatt tcagcgcccg cgcgacggag 4320 gcggagatcg ccggcctgtt tcgcgcggtc ggcgaggtgg cgcgcggcgc cggcgccgcc 4380 gagcccggtt accgcatcct gtccaactgc ggcgaggacg cgaaccagga ggttccgcac 4440 ctgcacatcc acgtcttcgc cggccgccgc ctcggtccga tgatcaccaa ggggtgagcc 4500 tcggcgtggc gatccacgtg aaacatcggc ctgaaacaga aggccgcggg ggaaaccccc 4560 ggcgcccttt tcttttggtc cggttctttt cgctcacacc atggcgcgga tctgccgctc 4620 cgccgcttcg ccgtcgatgc ggcagtagac ggcctggacg acgctgaagg caccgaaggc 4680 gaacagcccg gcggccacca ccccgagcag ccaggggccg tagggctgct gctggagaaa 4740 ctgcag 4746 // |
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