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EBI DbfetchID X61122; SV 3; linear; genomic DNA; STD; PRO; 5779 BP. XX AC X61122; XX DT 09-OCT-1992 (Rel. 33, Created) DT 27-APR-2004 (Rel. 79, Last updated, Version 7) XX DE Escherichia coli genes for eco57IM endonuclease and methyltransferase XX KW endonuclease; methyltransferase. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. XX RN [1] RC revised by [3] RA Vaisvila R.; RT ; RL Submitted (30-JUL-1991) to the EMBL/GenBank/DDBJ databases. RL R. Vaisvila, Inst. of Applied Enzymology Fermentas, Fermentu 8, Vilnius RL 232028, LITHUANIA. XX RN [2] RP 1-5779 RX DOI; 10.1093/nar/20.22.6051 RX PUBMED; 1334261. RA Janulaitis A., Vaisvila R., Timinskas A., Klimasauskas S., Butkus V.; RT "Cloning and sequence analysis of the genes coding for Eco57I type IV RT restriction-modification enzymes"; RL Nucleic Acids Res. 20(22):6051-6056(1992). XX RN [3] RC revised by [4] RA Janulaitis A.; RT ; RL Submitted (02-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Janulaitis A., UAB Fermentas, Graiciuno 8, LT-00241 Vilnius, LITHUANIA. XX RN [4] RP 1-5779 RA Janulaitis A.; RT ; RL Submitted (04-MAR-2004) to the EMBL/GenBank/DDBJ databases. RL Janulaitis A., UAB Fermentas, Graiciuno 8, LT-00241 Vilnius, LITHUANIA. XX FH Key Location/Qualifiers FH FT source 1..5779 FT /organism="Escherichia coli" FT /strain="original strain RFL57 cloned in RRI" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT -35_signal 634..640 FT /note="methyltransferase" FT TATA_signal 657..662 FT /note="methyltransferase" FT RBS 687..690 FT /note="methyltransferase" FT CDS 696..884 FT /transl_table=11 FT /note="SORF" FT /db_xref="UniProtKB/Swiss-Prot:P25287" FT /protein_id="CAA43432.1" FT /translation="MAKGESERIVLEVEPELKKALYSVLAMEQKTLKDWFVDKAQEHIC FT EKKSELIERFSKVDNEI" FT CDS 874..2508 FT /transl_table=11 FT /product="methyltransferase" FT /db_xref="GOA:P25240" FT /db_xref="InterPro:IPR002052" FT /db_xref="UniProtKB/Swiss-Prot:P25240" FT /protein_id="CAA43433.1" FT /translation="MKFKADQTSQKLRGGYYTPQNLADYVTKWVLSKNPKTILEPSCGD FT GVFIQAIANNGYNSNIELFCFELFDTEASKALERCKLNNFSNATITEGDFLVWANECLK FT KNKQIFDGALGNPPFIRYQFLERNFQEQAQLVFEHLDLKFTKHTNAWVPFLLSSLALLK FT QGGRIGMVIPSEISHVMHAQSLRSYLGHVCSKIVIIDPKEIWFEDTLQGAVILLAEKKQ FT YPDEASQGVGIVSVSGFEFLQEDPNVLFNDTAGINGETVEGKWTKATLSIDELQLIKRV FT IAHPDVRKFKDIAKVDVGRYCDGANNYFLVDNETVKLYKLERFAHPMFGRSQHCPGIIY FT DEKQHIENQEKGLPTNFLYIDEEFEYLSKSVKNYIKLGEVEEYHKRYKCRIRKPWFKVP FT SVYSTEIGMLKRCHDAPRLIHNRVRAYTTDTAYRVSSTVTSTENLVCSFLNPITVITAE FT LEGLFYGGGVLELVPSEIEKLYILIVEGLEHNVEELNLLIKDGQIERVIRQQGSLILGT FT LGFTQEENEKLVEIGRSLEIEGYVSRV" FT CDS complement(2570..5566) FT /transl_table=11 FT /product="endonuclease" FT /db_xref="GOA:P25239" FT /db_xref="InterPro:IPR007409" FT /db_xref="UniProtKB/Swiss-Prot:P25239" FT /protein_id="CAA43434.3" FT /translation="MNKKDQLQRLIDKYKSDIDYYRSARYNETQLRTDFLDQLFLILGW FT DITNAAGKPTNEREVLVEEGLKARAGENTKKPDYTFRLFSERKFFLEAKKPSVDISTTI FT EPALQVRRYGFTAKLKISVLSNFEYTAIYDCSNQVKETDSVANSRIKLYHFTELVDKFD FT EINNLIGRESVYTGHFDNEWSEIENKILRFSVDDLFLKQINDWRLLLANEFLQIKNELP FT EEKLNDLVQNYINSIVFLRVCEDRDLEEYETLYHFAQDKDFQSLVKKLKSSDKKYNSGL FT FSLEYIDELLSNANSCIWSIIEQLYFPQSTYSFSVFSSDILGNIYEIFLSEKVRIDELG FT NVKIQPKEEHIDRDVVTTPTHIVKEIIRNTVVEYCKGKSDIEILNSKFADIACGSGAFI FT IEAFQFIQDILIDYYIQNDKSKLQQISEHTYKLKFEVKREILCKCIYGIDKDYNATKAC FT TFGLLLKLLEGETTETIGKDTPILPALDTNILFGNSLIDSGDKVKQEDIFSINPFDLTN FT YQFDVIVGNPPYMATEHMNQLTPKELDIYKRKYKSAYKQFDKYFLFIERSIQILKEYGY FT LGYILPSRFIKVDAGKKLRKFLSENKYLSKLISFGSHQVFKNKTTYTCLLFLNKENHDN FT FSFYEVKDFKKWLTREDKYLLSSTYQTSSLDSDTWVLEKKINDILKLMFSKSEQLGNIV FT GKSNVANGIQTSANKYYIHKEIKSENGFIYFEYDGIEYHIEKELTRPYFETNRSGDDSF FT YTYKDVEPNSFVVYPYKKVGERIQFIEYDELKRQYPKLFEFLQVVKVHLNDKKRSIKPD FT PTGPNEWYRYGRSQALENCDVDQKLIVGILSNGYKYSIDNHRTFVSSGGTAGYSIINIP FT NNVRYSIYYIQAILTSKYLEWFASIYGDIFRGRFVARGTKVQTRMPIPTIDFDDPKQKE FT IHDTISSKQQYLNKLYSQTQKSADRDKIIFERQFEQEKIQMDYLIKNLFDLGDLDSEIP FT TVEDLYKNL" FT RBS complement(5573..5576) FT TATA_signal complement(5609..5615) FT /note="endonuclease" FT -35_signal complement(5643..5648) XX SQ Sequence 5779 BP; 1832 A; 985 C; 1030 G; 1932 T; 0 other; taaagtgtgg ttttaggccg aatttcaatt gattcatcat ttgtggaatg taactaatcg 60 acatgcttcc caaacagaaa aatcgcttct attcccaaca gaaattttac gaacatgagc 120 gcaattattt ctctgctagg ataattggtt tttagtaaat cgctacagca ggcaattgcc 180 aatataaaaa tctggcacaa gggtgaacag cgcgcgccac ataatccatt gttattgcta 240 tatattagcg gaatacataa atgaacagcc acgtcttttc gattatggtc gaaaatctac 300 gaacctctgc acagtttacg tagaactttt tttggccaca gagttcacag taccggccta 360 tatcgtggcg cttgaaggaa gatagagaaa gattaaagat ggtggatatt gagttatgca 420 aaactgcaac ttggataatg cagtgctact gcggcccgat tttggcagta tctttagtat 480 attcagagcg gtatagcctt acacttttat tccgttcggc tttactgcta agatatatgg 540 ctctgcagag ccttcccctt accatattgg ctagggattc ttctgacttt tcaaacggtt 600 gaaaagaatg ctgaaaggca agccagtttt ttctttagaa gctctgaaaa cccatgtatt 660 atatgtaaca taacatataa tacatgggtg ttaatatggc aaaaggtgaa tcagaaagaa 720 tcgtcctaga ggttgagcca gaactaaaaa aagctctcta ttcagttctt gcaatggaac 780 aaaaaactct taaagactgg tttgttgata aggctcaaga acatatatgc gagaaaaaat 840 cagagctcat agagagattt tcgaaggtag acaatgaaat ttaaagcaga tcaaacctca 900 caaaaactta gaggtggcta ttacacccca caaaatttag ctgattatgt aactaaatgg 960 gtcttaagta agaatcctaa aaccatactt gagccgagtt gtggtgatgg tgtgtttatt 1020 caggctattg ctaataatgg ttataactct aacattgagt tattttgctt tgaattgttc 1080 gacactgaag catctaaggc tctcgaacgc tgtaaactga acaatttttc taatgcaacg 1140 attactgagg gggattttct agtctgggct aatgaatgtc ttaagaaaaa taagcagata 1200 tttgatggag ctttaggtaa tcctccattc atccgttatc agttccttga aagaaatttc 1260 caagaacaag ctcagttggt cttcgaacat ttagatctaa aatttacaaa gcacactaat 1320 gcttgggttc cattcttact ttcttcatta gcccttctca aacaaggtgg aagaataggg 1380 atggtcatac catcggagat tagtcatgta atgcacgctc aatcattgag aagctactta 1440 ggtcatgttt gctctaagat tgtcatcatt gaccctaaag agatttggtt tgaagataca 1500 ttacaggggg ccgttattct ccttgcagag aaaaagcaat atccagatga agcatcgcaa 1560 ggtgtaggta ttgttagtgt tagtggtttt gaattccttc aagaagatcc aaacgttctg 1620 tttaatgata cggcaggaat aaatggtgaa acagttgaag gtaaatggac taaagcaaca 1680 ctcagtattg acgagcttca gctaattaaa cgggtaattg ctcatccaga tgttcgtaaa 1740 tttaaagata tagctaaagt tgatgtaggt aggtattgtg acggggccaa taattatttt 1800 cttgttgata atgaaacagt taaattatat aaattagaac gttttgctca ccctatgttt 1860 ggcagaagcc aacattgccc cggaattatt tatgatgaga agcagcacat tgaaaaccag 1920 gaaaagggtt taccaaccaa ttttttatat atagatgaag agtttgaata tctttctaaa 1980 agtgtcaaaa attacataaa gttaggtgaa gtagaggaat accacaagcg ttataagtgc 2040 cgtatacgta agccttggtt caaggtccca tctgtatata gcacagagat tggaatgtta 2100 aagcgttgtc acgatgcgcc acgcttaatt cataacagag ttagagcata taccactgat 2160 actgcatata gagtttcttc aacagttact agtactgaga acttagtgtg tagttttttg 2220 aaccctataa cggtcattac agccgaactc gaaggactct tttacggcgg tggcgttctt 2280 gaacttgtcc catccgagat agaaaaacta tatatcctta tcgttgaagg attagaacat 2340 aacgttgaag aacttaacct attgattaaa gatggtcaaa tagagagggt aattcgacaa 2400 caaggttcac taatcttagg cacgcttggt ttcactcaag aagaaaatga aaaacttgta 2460 gaaataggaa gaagcttaga gatagaaggc tacgtaagta gggtttaaaa agtcgggggc 2520 tcataaaaaa tcagtgcagc ttttagctca ttgattttat ctggtaacct tataggtttt 2580 tgtatagatc ttctactgtt ggaatctcgg aatctaaatc ccccaagtca aaaagattct 2640 taatcaaata atccatttgg attttttctt gttcaaattg cctttcgaat atgattttat 2700 ctctatcagc tgatttttga gtttggctat acagtttatt caaatactgt tgctttgaac 2760 tgatcgtatc atgtatttct ttctgttttg gatcatcaaa atcaatagta ggaataggca 2820 ttctagtttg aacttttgtc ccacgagcaa caaatctacc tctaaaaata tcaccatata 2880 ttgaagcaaa ccactcaagg tactttgatg taaggattgc ttggatataa tagatagagt 2940 atctaacgtt attaggaatg ttgataatgc tatagcccgc agtgcctcca gaagaaacaa 3000 aagttctatg gttatcaatg ctgtacttat atccattaga tagaatacca acaatcagct 3060 tctgatcaac atcgcagttt tctaatgctt ggctacgccc gtatctgtac cattcattag 3120 gcccagttgg gtcaggctta atagagcgtt tcttatcatt caaatggact tttacaacct 3180 gcaggaactc aaataactta ggatattgac gttttagttc atcgtattca atgaactgga 3240 ttctttcacc cactttcttg tatgggtaaa caacaaatga attaggttca acgtctttat 3300 aagtatagaa actatcatca cctgaacgat tcgtttcgaa gtaagggcga gttaattcct 3360 tttcaatgtg gtattcaatt ccatcatatt caaaataaat aaaaccattt tcagatttga 3420 tttctttatg aatgtagtat ttatttgcac ttgtctgaat gccatttgca acattacttt 3480 ttcctacaat gttacctagc tgttcagatt ttgaaaacat tagcttcaaa atgtcattga 3540 ttttcttttc tagaacccaa gtgtcagaat caagagaact ggtttgatat gtgcttgata 3600 ataaatattt atcttcacga gttaaccact tcttgaagtc ttttacttca taaaaagaaa 3660 agttatcgtg attttcttta tttaaaaata acaaacaagt gtaagtcgtt ttattcttga 3720 atacttgatg agaaccaaaa gaaataagtt tagataaata tttattttct gataaaaact 3780 tgcgtagttt tttacctgca tctactttta tgaacctaga cggaagaata tagcctaaat 3840 agccgtactc tttaagaatt tgtattgaac gctcaataaa taaaaagtat ttatcgaatt 3900 gcttataagc agatttatat ttcctcttgt aaatatctaa ttcctttggt gttaactgat 3960 tcatatgctc agtcgccata taaggaggat ttccaacgat cacatcaaat tggtagttag 4020 tcaaatcaaa tggattaatt gaaaaaatat cttcttgttt aactttgtct ccactatcta 4080 tcaagctatt cccgaataga atgttcgtat ccagtgcagg caaaataggg gtgtctttac 4140 caatggtttc tgtagtttcg ccttcgagaa gctttagcaa taaaccaaag gtacaagctt 4200 ttgttgcatt atagtcttta tcaatcccat atatacattt acatagaatt tctcttttta 4260 cttcaaattt aagtttgtat gtatgttcag atatttgctg aagtttagat ttgtcatttt 4320 gtatataata atcaataaga atatcctgga tgaattggaa tgcctcaata ataaaagcac 4380 ctgagccaca agcgatatca gcaaattttg aattcaatat ctcaatatct gacttgcctt 4440 tgcaatattc tacgacagta tttctaatga tttcttttac aatatgagtt ggtgtagtaa 4500 caacgtctct atcgatatgt tcctctttcg gttgaatctt aacgtttcca agttcatcaa 4560 tacgaacttt ctcactcagg aatatttcat atatattacc taaaatatca gaagagaaaa 4620 cagagaaaga gtatgtgctt tgtgggaagt ataattgttc tatgatgctc caaatgcaag 4680 agttagcatt gcttaataac tcatcaatat attctagcga gaataatcct gaattatatt 4740 tcttgtctga ggatttcaat tttttgacta aagattgaaa atccttatct tgtgcaaagt 4800 gataaagagt ttcgtattct tccaagtctc tatcttcaca gactctaagg aaaacaatac 4860 tgttaatata attttgtaca agatcattta atttttcttc tggaagttcg ttttttatct 4920 gcaaaaattc attagcaaga agtaagcgcc aatcattaat ttgcttgagg aatagatcat 4980 caacactgaa tctcagtatc ttattttcaa tttctgacca ttcattgtca aaatgaccag 5040 tgtaaacact ttctcgaccg ataagattat taatttcatc aaacttatct acgagttcag 5100 tgaagtgata caacttgatt ctagagtttg ctactgaatc agtttcttta acctgattag 5160 agcaatcata aattgccgta tactcgaaat tcgacaacac agaaattttt aatttggctg 5220 tgaatccgta tcgtctaact tgtagagctg gttctatagt agttgatata tcaacactag 5280 gctttttggc ttcaaggaaa aatttacgct cagagaagag tctaaacgtg tagtctggct 5340 ttttagtatt ttcgcctgct cgggctttta aaccttcctc tacaagcact tcgcgttcgt 5400 ttgttggttt tcctgctgcg ttcgttatgt cccaaccgag aataaggaat agttgatcta 5460 aaaaatccgt tcttagttga gtttcgttgt atctggcaga acgataataa tcaatatcgc 5520 ttttgtattt atcaattagt ctttgtaact gatctttttt attcatctat ttctcacaac 5580 gatatttatc aaaaagtaga tttctaatga ttgtaattct tgtaccgaag tattgttagt 5640 caagttatcc taacagttcc attctttacg tctcttgttg tgctgcacag attttgctgt 5700 cactattttt acctcaaaaa ccacagtaac actaccaata ggtggctttt cgatgatgac 5760 tgcaaggaaa gtgtggtat 5779 // |
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