EBI Dbfetch

ID   X61088; SV 1; linear; genomic DNA; STD; PRO; 1429 BP.
XX
AC   X61088;
XX
DT   02-AUG-1991 (Rel. 28, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 6)
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DE   E.carotovora pel9.5 gene for endo-pectate lyase
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KW   endo-pectate lyase; pectatelyase; pel gene; pel9.5 gene.
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OS   Pectobacterium carotovorum
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Pectobacterium.
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RN   [1]
RP   1-1429
RA   Antonov L.S.;
RT   ;
RL   Submitted (25-JUL-1991) to the EMBL/GenBank/DDBJ databases.
RL   L.S. Antonov, Lab for Mol. Biology of Plant Stress, Plant Mol. Biology
RL   Building, VPI 7 SU, Blackburg, VA 24061-0330, USA
XX
RN   [2]
RA   Antonov L.S., Lacy G.H.;
RT   "Erwinia carotovora subspecies carotovora: Structure of a functional
RT   pelX-like pectate lyase gene is distinct from other pelBC genes";
RL   Mol. Microbiol. 0:0-0(0).
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CC   According to Prof. George H. Lacy the sequence reported may be
CC   incorrect. The sequence will be reinvestigated in order to allow him to
CC   support their legitimacy.
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FH   Key             Location/Qualifiers
FH
FT   source          1..1429
FT                   /organism="Pectobacterium carotovorum"
FT                   /strain="EC14"
FT                   /mol_type="genomic DNA"
FT                   /clone="pDR40"
FT                   /cell_line="lacy culture collection accession L-543"
FT                   /db_xref="taxon:554"
FT   promoter        22..49
FT   repeat_region   23..90
FT                   /rpt_type=DIRECT
FT   misc_binding    160..175
FT                   /bound_moiety="KdgR"
FT   RBS             221..226
FT   sig_peptide     235..300
FT   CDS             235..1296
FT                   /transl_table=11
FT                   /gene="pel9.5"
FT                   /product="pectate lyase"
FT                   /EC_number="4.2.2.2"
FT                   /db_xref="GOA:Q04086"
FT                   /db_xref="HSSP:1AIR"
FT                   /db_xref="InterPro:IPR012334"
FT                   /db_xref="UniProtKB/TrEMBL:Q04086"
FT                   /protein_id="CAA43402.1"
FT                   /translation="MKYLLPSAAAGLLLLAAQPTMAANTGGYATTDGGDVSGAVKKTAR
FT                   SLQEIVDIIEAAKKDSSGKAVKGGPYPLVITYNGNEDALIKAAEANICGQWSKDPRGVE
FT                   IKEFTKGITILGTNGSSANFGIWMVNSSNVIVRNMRFGYMPGGAKDGDAIRIDNSPNVW
FT                   IDHNEIFAKNFECAGTPDNDTTFESAVDIKKASTNVTVSYNYIHGVKKVGLSGSSNTDT
FT                   GRNLTYHHNIYSDVNSRLPLQRGGQVHAYNNLYDGINSSGFNVRQKGIALIIRNMHNAL
FT                   NPVATRDGDANFGTWDLRNNNITSPADFAKYNITWDKATTPYINAEDWKSTGTFSAVPS
FT                   SYSPFSAQCKDKW"
FT   mat_peptide     301..1293
FT                   /gene="pel9.5"
FT                   /product="pectate lyase"
FT                   /EC_number="4.2.2.2"
XX
SQ   Sequence 1429 BP; 409 A; 349 C; 321 G; 350 T; 0 other;
     agcgaacgta aattcaccca attgtcttct attatacaat taatatattc gatgtcttct        60
     attatacaat taatatattc gatgtcttat cctgcattac ttgctttttc tgttaacact       120
     tagggacggc tttatagaca aaccttaatt tcatttttgt tgaaacagct atcttattac       180
     acagggtgtg tagtgatcaa tacccaaaaa attctatgtc aaggagagta cataatgaaa       240
     tacctactgc cttctgcagc agctgggctg ttgctgctcg ctgcccaacc gacaatggcg       300
     gcaaatacgg ggggttatgc caccactgac ggcggcgacg tttctggtgc ggtgaaaaaa       360
     acggcgcgtt ctctgcaaga gatcgtcgat atcattgaag ccgcgaaaaa ggactcaagc       420
     ggtaaagcgg tcaaaggcgg accttacccg ctcgttatta cctacaacgg taatgaagat       480
     gcgctaatca aagccgccga agctaacatc tgcggccagt ggagcaaaga tccgcgcggt       540
     gtggaaatca aagagttcac caaagggatc accattctcg gaaccaacgg ttcttccgct       600
     aacttcggta tctggatggt taactcttcc aacgttatcg tacgtaacat gcgcttcggc       660
     tatatgcctg gcggtgcaaa agatggtgat gccatccgta tcgataattc accgaacgtc       720
     tggatcgacc acaacgagat cttcgccaag aacttcgaat gtgccggtac gccagacaac       780
     gacaccacct ttgaatccgc ggttgatatc aagaaagcct caaccaacgt caccgtgtcg       840
     tacaactata tccatggcgt gaaaaaggtc gggttgagcg gttcaagcaa cacggataca       900
     gggcgtaacc tgacttacca tcacaatatt tacagcgatg ttaactcacg tctgccgctg       960
     caacgtggtg gccaagtcca tgcttataac aacctgtatg acggcatcaa cagttcaggc      1020
     tttaacgtcc gtcagaaagg gatcgcattg atcatacgca acatgcacaa tgcactcaac      1080
     ccggtggcca cacgtgacgg tgatgccaac ttcggtactt gggacctacg taataacaac      1140
     attaccagcc cagccgactt tgctaaatac aatatcactt gggataaagc aaccacacct      1200
     tacatcaatg ctgaagactg gaaaagcacc ggcacgttct cagccgtccc atctagctat      1260
     tctccatttt ctgcacagtg caaggataaa tggtaaagct atgcgtgcgt aggtaaaaac      1320
     gaggagtact gagagtagcc aaatgtaaat aaaagcgtta gcccgatgca attcaatcta      1380
     cgtagggttc ttcctggagc gaagtgcttc gcctcgttgt tgatagggg                  1429
//