 |
|
EBI DbfetchID X61087; SV 1; linear; genomic DNA; STD; PRO; 1388 BP. XX AC X61087; XX DT 02-AUG-1991 (Rel. 28, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 8) XX DE E.carotovora pel10.5 gene for endo-pectate lyase XX KW endo-pectate lyase; pectatelyase; pel gene; pel10.5 gene. XX OS Pectobacterium carotovorum OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Pectobacterium. XX RN [1] RP 1-1388 RA Antonov L.S.; RT ; RL Submitted (25-JUL-1991) to the EMBL/GenBank/DDBJ databases. RL L.S. Antonov, Lab for Mol. Biology of Plant Stress, Plant Mol. Biology RL Building, VPI 7 SU, Blackburg, VA 24061-0330, USA XX RN [2] RA Antonov L.S., Lacy G.H.; RT "Erwinia carotovora subspecies carotovora: Structure of a functional RT pelX-like pectate lyase gene is distinct from other pelBC genes"; RL Mol. Microbiol. 0:0-0(0). XX CC According to Prof. George H. Lacy the sequence reported may be CC incorrect. The sequence will be reinvestigated in order to allow him to CC support their legitimacy. XX FH Key Location/Qualifiers FH FT source 1..1388 FT /organism="Pectobacterium carotovorum" FT /strain="EC14" FT /mol_type="genomic DNA" FT /clone="pDR40" FT /cell_line="lacy culture collection accession L-543" FT /db_xref="taxon:554" FT promoter 39..67 FT repeat_region 69..101 FT /rpt_type=INVERTED FT misc_binding 102..118 FT /bound_moiety="KdgR" FT misc_feature 113..126 FT /note="dyad symmetry" FT RBS 183..188 FT sig_peptide 198..263 FT CDS 198..1301 FT /transl_table=11 FT /gene="pel10.5" FT /product="pectate lyase" FT /EC_number="4.2.2.2" FT /db_xref="GOA:Q04085" FT /db_xref="HSSP:1AIR" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/TrEMBL:Q04085" FT /protein_id="CAA43401.1" FT /translation="MKYLLPTAAAGLLLLAAQPAMAANTGGYATTDGGEVSGAVKKTAR FT SMKEIVDIIEAAQVDSKGKKVKGGAYPLIITYSGNEDSLIKAAEKNICSQWSKDARGVE FT IKEFTKGTYHPWNQRIVRQLRSLDCETLLKFWYVICALVTCRAARKHGDAIRIDNTPNV FT WIDHNEIFAKNFECAGTKDGDTTFESAIDIKKASTNVTISYNYIHGIKKVGLSGLSQRQ FT RDDRLTRYRFFASPDQRSRCGFCARRHSSSRRAGRALPSLRYRVDCGTKRISLRRCFSS FT YPVIYPPTNFASLRFNPRRPQAHISASNSTCIRIIITDSRRCCEDAVTFSSCISSAATA FT ISAPHYYQYVISKGKCQQAFPYHVTLH" FT mat_peptide 264..1298 FT /gene="pel10.5" FT /product="pectate lyase" FT /EC_number="4.2.2.2" XX SQ Sequence 1388 BP; 368 A; 348 C; 312 G; 360 T; 0 other; ccaatcatca gtattacaaa tgtttcaccg caatacattt aacatttcac ccttgaactg 60 atcttatttt tgaccacact tcccttggtt ttccatcaaa attgaaattc atttttgttg 120 aaaaatttat acttgttaca tcgggcatag ggatcgataa atgcccatga aaattctatt 180 ccaaggagac agtgataatg aaatacctat tgcctacggc agctgccgga ttgctattac 240 tcgcggctca acccgcaatg gccgcaaata cgggcggcta tgccactacg gatggtggag 300 aagtatccgg tgccgtgaaa aaaacagcac gttctatgaa agaaattgtg gatattattg 360 aagccgcgca agtggattca aaaggcaaga aagtcaaagg cggtgcttac ccgctcatca 420 tcacctatag cggtaatgaa gattcattaa tcaaagcggc tgaaaagaat atctgcagcc 480 agtggagtaa ggatgcccgc ggtgtggaaa tcaaagagtt caccaaaggg acttaccatc 540 cttggaacca acggatcgtc cgccaacttc ggagtctgga ttgtgagact cttctaaagt 600 tctggtacgt gatatgcgct ttggttacat gccgggcggc gcgcaagcat ggcgatgcca 660 ttcgtatcga taacacgccg aacgtctgga ttgaccacaa cgagatcttc gcgaaaaact 720 ttgaatgcgc aggtacaaaa gacggtgaca cgacattcga gtccgcgatt gatattaaga 780 aagcctcgac caacgtgacc atttcgtaca actacattca tggcatcaaa aaggttgggc 840 tcagtggcct ttcacagcga cagcgcgacg accgactaac acgctaccgg ttttttgcct 900 cccctgatca acgttcaaga tgcggttttt gtgctcgacg acatagttcg tcccgccggg 960 ccggcagggc tctgccttca ctacgatatc gcgttgactg tggcactaag cgtatttcac 1020 tgcgacgatg cttttcgtct tatccagtga tttatccacc cactaacttt gcctcgctga 1080 ggttcaaccc ccgacgacca caggcacaca tttccgcgtc caacagcact tgcatacgca 1140 ttatcattac tgactcgcgg cgttgctgtg aagacgctgt gacattcagt tcgtgtattt 1200 catccgcagc gacagcgatt tcagcgccgc actattacca gtatgtaata tcaaagggca 1260 aatgccagca ggcctttccg tatcacgtta cgttgcatta gtgccgctgc tacttgttcg 1320 ccattttacg ccttgtacac tttgtaatta gtgattcttc ggtagtactg tcgagaatgc 1380 gaaaccca 1388 // |
|