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EBI DbfetchID X60420; SV 1; linear; genomic DNA; STD; PRO; 3434 BP. XX AC X60420; S43726; XX DT 27-OCT-1992 (Rel. 33, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 21) XX DE Erwina herbicola pheA, tyrA, and aroF genes XX KW aroF gene; bifunctional protein; chorismate mutase/prephenate dehydratase; KW DAHP synthase; P-protein; pheA gene; T-protein; tyrA gene; KW tyrosine-sensitive DAHP synthase isoenzyme. XX OS Pantoea agglomerans OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Pantoea. XX RN [1] RP 1-3434 RA Xia T.; RT ; RL Submitted (11-SEP-1991) to the EMBL/GenBank/DDBJ databases. RL T. Xia, University of Florida, Dep of Microbiology and Cell Sciences, 3103 RL McCarthy Hall, IFAS, Gainesville, FL 32611, USA XX RN [2] RP 1-3434 RX PUBMED; 1512561. RA Xia T., Zhao G., Fischer R.S., Jensen R.A.; RT "A monofunctional prephenate dehydrogenase created by cleavage of the 5' RT 109 bp of the tyrA gene from Erwinia herbicola"; RL J. Gen. Microbiol. 138(7):1309-1316(1992). XX FH Key Location/Qualifiers FH FT source 1..3434 FT /organism="Pantoea agglomerans" FT /mol_type="genomic DNA" FT /clone_lib="Sau 3A:pUC18" FT /clone="pJX181" FT /db_xref="taxon:549" FT promoter 243..272 FT /gene="pheA" FT terminator 395..416 FT /note="pheA attenuator terminator" FT CDS 445..1608 FT /transl_table=11 FT /gene="pheA" FT /product="chorismate mutase" FT /product="prephenate dehydratase" FT /EC_number="5.4.99.5" FT /EC_number="4.2.1.51" FT /db_xref="GOA:Q02286" FT /db_xref="HSSP:1ECM" FT /db_xref="InterPro:IPR018528" FT /db_xref="UniProtKB/Swiss-Prot:Q02286" FT /protein_id="CAA42949.1" FT /translation="MNPDNPLLALRDKISAVDKKLLTLLAERRLLAVEVAQAKLATHRP FT IRDVERERALLENLIVLGKAHNLDAHYITRLFQLVIEDSVLTQQALLQKNLNHPHAHAA FT RIAFLGPKGSYSHLAARNYASRHFDSMVECGCLKFHDIIKQVENGVADYAVMPIENTSS FT GSINDVYDLLQQTSLSIVGELTLPIDHCVLVNGPTDLQQIETVYSHPQPFQQCSQFINR FT FPHWKIEYTESTAAAMEKVAALNSPKVAALGSEAGGELYQLQVLERNLANQQQNHTRFI FT VLARKAIEVSDQVPAKTTLIMATGQQAGALVDALLVLRQHNLIMSKLESRPINGNPWEE FT MFYIDVQGNLQSERMQQALQELQTMTRSLKVLGCYPSENVVPAEPGR" FT terminator 1616..1645 FT /gene="pheA" FT terminator complement(1616..1645) FT /gene="tyrA" FT CDS complement(1665..2786) FT /transl_table=11 FT /gene="tyrA" FT /product="chorismate mutase" FT /product="prephenate dehydrogenase" FT /EC_number="5.4.99.5" FT /EC_number="1.3.1.12" FT /db_xref="GOA:Q02287" FT /db_xref="InterPro:IPR003099" FT /db_xref="UniProtKB/Swiss-Prot:Q02287" FT /protein_id="CAA42950.1" FT /translation="MVAELTALRDQIDSVDKALLDLLAKRLELVAEVGEVKSRYGLPIY FT VPEREASMLASRRKEAEALGVPPDLIEDVLRRVMRESYTSENDKGFKTLCPELRPVVIV FT GGKGQMGRLFEKMLGLSGYTVKTLDKEDWPQAETLLSDAGMVIISVPIHLTEQVIAQLP FT PLPEDCILVDLASVKNRPLQAMLAAHNGPVLGLHPMFGPDSGSLAKQVVVWCDGRQPEA FT YQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVNLDQLLALS FT SPIYRLELAMVGRLFAQDPQLYADIIMSSESNLALIKRYYQRFGEAIALLEQGDKQAFI FT ASFNRVEQWFGDHAKRFLVESRSLLRSANDSRP" FT CDS complement(2792..>3434) FT /codon_start=2 FT /transl_table=11 FT /gene="aroF" FT /product="tyr-sensitive DAHP synthase isoenzyme" FT /EC_number="4.1.2.15" FT /EC_number="5.4.99.5" FT /db_xref="GOA:Q02285" FT /db_xref="HSSP:1HFB" FT /db_xref="InterPro:IPR006219" FT /db_xref="UniProtKB/Swiss-Prot:Q02285" FT /protein_id="CAA42951.1" FT /translation="DPNSPQYLGDLFSWSAIGARTTESQTHREMASGLSMPVGFKNGTD FT GSLGTAINAMRAAAMPHRFVGINQAGQVCLLQTQGNPDGHVILRGGKAPNYGPEDVAQC FT EKEMLKAGLRPALMIDCSHGNSNKDYSRQPGVAESAIAQIKDGNRSIIGLMLESHINEG FT NQSSEQPRSEMKYGVSVTDACINWEVTETLLREMHQDLQGVLSARLSQEV" XX SQ Sequence 3434 BP; 793 A; 988 C; 881 G; 772 T; 0 other; gaattcgtgg ctgacgccac tattaacaca cctaacggga ctctggtggc cagcgccaag 60 cacgatgata tgtataccgc tatcaacgat ttaattgcca agctcgagcg tcagctgaat 120 aaagttcagc acaagtctga agcacgccgc gccagtgcca gcgtgaaaga catcattccc 180 gccgacgaag agtcctgatt ttcttaacgc tgtttcttaa cgcgcctgcg ggcgggtttt 240 tattgacagc ttttaagcag tacggttact ctcaggtcac tctcttttcg gacatgacaa 300 tgaaacctga tctgtttttc ttcgctttct tttttacctt cccctgaacg ggaggccatt 360 cgtcgtgtga aaatgaaagc gaagacgaac accgaagcct cccaaccggg aggctttttt 420 ttggatagca tacaggtgag ccctatgaat cccgataatc cattgctggc gctacgcgac 480 aaaatcagcg ccgtggataa aaaacttctg acgctgctgg ccgagcgtcg tttgctggca 540 gtggaagttg cacaagcgaa actggcgacg catcgcccga ttcgggatgt ggagcgcgaa 600 cgcgcgctac tggagaatct catcgtactg ggtaaggcac acaacctgga tgcgcactac 660 atcacccgtc tgttccagct ggtaattgaa gattccgtgc tgactcagca agcgctgctg 720 caaaaaaacc tcaaccatcc ccatgcgcat gccgcccgta tcgcctttct gggcccgaaa 780 ggctcttatt cgcatcttgc agcacgcaac tacgcgtcgc gccatttcga tagcatggtg 840 gagtgtggct gcctgaagtt tcacgacatc atcaaacagg tcgaaaatgg cgtagctgat 900 tacgccgtga tgccgattga gaataccagt tcaggctcga tcaacgatgt ctacgatctg 960 ctgcaacaga ccagcctctc aatcgtgggc gagctgacat tgcctatcga ccactgcgtg 1020 ctggtgaatg gccccaccga tcttcagcag attgagacgg tttacagcca tcctcaaccg 1080 ttccagcagt gcagccagtt tattaatcgt ttcccgcact ggaaaatcga atacaccgag 1140 agtaccgcgg cagcgatgga gaaagtggct gcgctcaact cacctaaggt ggcggcgctg 1200 ggcagtgagg ccggcggcga actgtatcag ctgcaggtgc tggaacgtaa tctggcgaat 1260 cagcagcaga accacacccg ctttatcgtg ctggcccgca aagcaatcga ggtttctgac 1320 caggtgccag cgaaaaccac gctgattatg gcaaccggcc agcaggcggg tgcgctggtc 1380 gacgcgctgc tggtactgcg tcagcacaac ctgattatga gcaagctgga gtcccgcccg 1440 attaatggta atccgtggga agagatgttt tacattgatg tgcagggcaa cctgcaatca 1500 gagcggatgc agcaggcgct gcaggaatta cagaccatga cccgctctct gaaggtactg 1560 ggctgttacc ccagcgaaaa tgtggtgccc gctgaacctg gccggtaaaa agaagaaaaa 1620 aggcatccaa ctggatgcct tttttattga tggatggctg tcatttatgg gcggctgtca 1680 ttggccgatc gcaacaggct tcggctttcg accaggaagc gttttgcgtg atcgccaaac 1740 cactgttcaa cccggttaaa gctggcgata aacgcctgct tgtcgccctg ctccagcagc 1800 gcaatcgctt caccaaaccg ctggtaatag cgttttatca gcgccagatt actctctgaa 1860 gacatgatga tatccgcata gagttgcgga tcctgagcga acaaccgccc caccatcgcc 1920 agttcaagcc ggtaaatggg cgacgagagc gccagcagct gatccagatt gacgttctct 1980 tcggctaaat gcagaccata agcgaaggta gcaaagtgac gcagcgcctg aatgaatgcc 2040 atgttctggt catgctcaac agcgctgata cgatgcagac gcgcacccca gacctgaatc 2100 tgctccagga accactgata cgcttccggt tgtcttccat cacaccagac caccacctgt 2160 tttgccaggc tgccgctgtc cgggccaaac atcggatgca gacccagtac aggcccgtta 2220 tgggcagcca gcattgcctg cagaggccgg tttttgactg acgccagatc gaccagaata 2280 caatcttccg gcagtggtgg cagttgggca atcacctgct cggtcaggtg aatcggcacg 2340 ctaatgatca ccattccggc atcgctgagc agagtctcag cctgaggcca gtcctcttta 2400 tccagcgttt taaccgtgta gcctgatagc ccgagcattt tttcaaacag ccggcccatc 2460 tggcccttac caccgacaat caccaccggg cgcagttcag gacagagggt tttaaagcct 2520 ttatcattct cgctggtata ggattcccgc atcacgcgac gcagcacatc ctcaatcaga 2580 tccggtggta cgccgagcgc ttcggcctct ttgcgacgcg aagccagcat cgacgcctca 2640 cgctcaggca catagatagg caggccgtaa cggctcttca cctcacctac ctcggccacc 2700 agttccagtc gcttagccag cagatccagc agcgctttat ctacactgtc aatttgatcg 2760 cgtaacgcgg tcagttcagc caccattgct cttacacctc ttgtgacaga cgcgccgaca 2820 gcactccctg cagatcctga tgcatttcac gcagcaacgt ttcggtcact tcccagttga 2880 tgcatgcatc ggttacggaa acgccatact tcatttcgct gcgtggctgc tcagaagact 2940 ggttaccttc attgatatgg ctttccagca tcaagccaat gattgaacgg ttcccgtctt 3000 tgatctgcgc aatagcggat tccgctacgc caggctgacg gctgtagtct ttgttcgaat 3060 taccatggct gcaatctatc attaaggctg gacgcagtcc cgccttgagc atctcttttt 3120 cacactgcgc gacatcttca ggaccgtagt tcggcgcttt gccgccacgc aggatcacgt 3180 gaccatccgg attgccctga gtctgcagca ggcagacctg accggcctga ttaatcccga 3240 caaaacggtg tggcattgcg gcagcacgca tggcgttgat tgccgttccc agactgccat 3300 cggtgccatt tttaaagcct accggcatcg acaggcccga ggccatttca cggtgcgtct 3360 gcgattcagt ggtacgggcg ccaatggcag accagctgaa caggtcgccc agatattgcg 3420 gactattggg atcc 3434 // |
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