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EBI DbfetchID X59944; SV 1; linear; genomic DNA; STD; PLN; 5081 BP. XX AC X59944; X55789; XX DT 01-JUL-1992 (Rel. 32, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 5) XX DE P.americana cel1 gene for cellulase (endo-beta-1,4-glucanase) XX KW cel1 gene; cellulase; endo-beta-1,4-glucanase. XX OS Persea americana (avocado) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; magnoliids; Laurales; Lauraceae; Persea. XX RN [1] RP 1-5081 RA Cass L.G.; RT ; RL Submitted (04-JUN-1991) to the EMBL/GenBank/DDBJ databases. RL L.G. Cass, University of California, Dept of Biological Sciences, Santa RL Barbara CA 93106, USA XX RN [3] RP 8-5081 RX DOI; 10.1007/BF00315799 RX PUBMED; 2259345. RA Cass L.G., Kirven K.A., Christoffersen R.E.; RT "Isolation and characterization of a cellulase gene family member expressed RT during avocado fruit ripening"; RL Mol. Gen. Genet. 223(1):76-86(1990). XX CC See M17634 for overlapping sequence XX FH Key Location/Qualifiers FH FT source 1..5081 FT /organism="Persea americana" FT /cultivar="Haas" FT /mol_type="genomic DNA" FT /dev_stage="ripe fruit" FT /clone_lib="EMBL3" FT /clone="CEL1-6" FT /db_xref="taxon:3435" FT CDS join(1624..1833,1922..2131,2923..3123,3210..3302, FT 3408..3569,3650..3761,4159..4322,4409..4741) FT /gene="cel1" FT /product="cellulase" FT /EC_number="3.2.1.4" FT /db_xref="GOA:P05522" FT /db_xref="HSSP:1IA6" FT /db_xref="InterPro:IPR018221" FT /db_xref="UniProtKB/Swiss-Prot:P05522" FT /protein_id="CAA42569.1" FT /translation="MDCSSPLSLFHLLLVCTVMVKCCSASDLHYSDALEKSILFFEGQR FT SGKLPTNQRLTWRGDSGLSDGSSYHVDLVGGYYDAGDNLKFGLPMAFTTTMLAWGIIEF FT GCLMPEQVENARAALRWSTDYLLKASTATSNSLYVQVGEPNADHRCWERPEDMDTPRNV FT YKVSTQNPGSDVAAETAAALAAASIVFGDSDSSYSTKLLHTAVKVFEFADQYRGSYSDS FT LGSVVCPFYCSYSGYNDELLWGASWLHRASQNASYMTYIQSNGHTLGADDDDYSFSWDD FT KRVGTKVLLSKGFLQDRIEELQLYKVHTDNYICSLIPGTSSFQAQYTPGGLLYKGSASN FT LQYVTSTAFLLLTYANYLNSSGGHASCGTTTVTAKNLISLAKKQVDYILGQNPAKMSYM FT VGFGERYPQHVHHRGSSLPSVQVHPNSIPCNAGFQYLYSSPPNPNILVGAILGGPDNRD FT SFSDDRNNYQQSEPATYINAPLVGALAFFAANPVTE" FT mRNA join(1508..1833,1922..2131,2923..3123,3210..3302, FT 3408..3569,3650..3761,4159..4322,4409..5005) FT /gene="cel1" FT /experiment="experimental evidence, no additional details FT recorded" FT exon 1508..1833 FT /number=1 FT exon 1922..2131 FT /number=2 FT exon 2923..3123 FT /number=3 FT exon 3210..3302 FT /number=4 FT exon 3408..3569 FT /number=5 FT exon 3650..3761 FT /number=6 FT exon 4159..4322 FT /number=7 FT exon 4409..5005 FT /number=8 FT intron 1834..1921 FT /number=1 FT intron 2132..2922 FT /number=2 FT intron 3124..3209 FT /number=3 FT intron 3303..3407 FT /number=4 FT intron 3570..3649 FT /number=5 FT intron 3762..4158 FT /number=6 FT intron 4323..4408 FT /number=7 FT TATA_signal 1475..1480 FT CAAT_signal 1438..1450 FT misc_feature 1153..1172 FT /note="GAGA box" XX SQ Sequence 5081 BP; 1396 A; 1083 C; 1029 G; 1573 T; 0 other; gtcgacgctt ttaattcagg tggctctttc ttttgattag cccacctgag tcttttaacg 60 gctggatggg aatgtccgca tgcagatatt acctagggag aaaacttaag ctccaaacaa 120 gttacaacaa tcaggcttaa gtgaaagttt aacaagaaaa taaagggtaa gactctgaaa 180 gagtgttttt agtgtgagtg ttagggtaat gagttttctc ccttttgttc tcactccaac 240 gtccagtttt ctgcagctga tcccgtgaac agcaccggcg gaatcatagg agggccggca 300 ggggccattg gcccctccac tgccacccaa aatttcaaaa aacccaccct ttgtcttttc 360 aaaatttata atctttttac attatggtcc ccccaaagtt ttcataaaag cctattttga 420 aaactgcatt cattttttat gtggcccttt ccaaaaaaat ttctacatag tggccccctc 480 aaggaagaat tcatgggtct gcccctgcaa ataggtgaga tatgggggga attagtgggt 540 caaggttaga ttttgctgca tctaattgcg aaaggttaga gatataaggt ggaaatttgg 600 atgagacctt gactgtctta taatttaatt tattaaggtg atggctgagt attcatttta 660 taacttcctt tagttcattt tttaaccaaa cggaacatta ttcccataat tttaaacaac 720 gcccccccgc gccccccaac ttttaaagaa aattctatga tttttcacat acagcccctc 780 caaaatttct aaaaaacccc tgtttcttta gaaaattatt tcttttacat atggacccct 840 gacatttcaa aaaatctact atatattttg aaaattacat acatttctta tatgtgggct 900 cccaagaact tcttttctac ccagtgcccc cggcctccca ataatttatt ttctactgtt 960 ttctcaaaaa aatggacttc tcttaagagt tttgcacgta tgcttcaccc ttctttctta 1020 agctagagaa ttgctaactt tatgcagtta gttgaaattt cttccatcgc tggttggttt 1080 taaaaaaggg ttgcaggcat gcagcttgaa ttttaaaaaa gggttgcagg tatggcgctc 1140 gaacgtaaat atgagagaga gagagagaga gattgagatt ttgaagtcct aaaagctaaa 1200 agcggacaat tacatcaagt cctttcacag tgatcttaac aattagatgg acgcagatgg 1260 gatggtcgcg tctcccacac gtagttgtga gctaattgag agcaccgctt attacttatt 1320 agtaccaagc cagttgtacg aggaaccaag caccgtatcg actttttaag ccttggcgtt 1380 tgtagttttg tggagagaga ttgagatttt accaccgttt cttccctcca acgaccaact 1440 gctattttga cagccctttc tgctcctttt cctctataaa tagagcagtc tctacaccaa 1500 cactcttcat actcattcaa gcctgctctg gtagtagcag tactagtagt attggtaata 1560 gaaatagtag aatatcgaca ctagttgttg cagttgttca ctccttttga gggttgcaga 1620 gagatggatt gtagtagccc actttctttg tttcatcttt tgttagtatg cacagtgatg 1680 gttaaatgct gctctgcttc tgatttgcat tactctgatg cactcgaaaa atcaatcctc 1740 tttttcgaag ggcaaaggtc ggggaaatta cccaccaacc agcgtctcac atggaggggt 1800 gactctggtt tatcagatgg ctcctcttat catgtatgtg tttttctgat gctataactt 1860 cactgtccat taactttgtc atatgtatac agagattgaa cttttgattt gttgtatgca 1920 ggtggattta gtaggcggat actatgatgc aggggataat ctgaagtttg gtctgcctat 1980 ggccttcact acgacaatgc tggcatgggg tatcattgag ttcggctgct tgatgccgga 2040 gcaggtagag aatgcaagag cggcccttcg ctggagcacg gattatctac tcaaggcctc 2100 tacagccact tccaattctt tatatgtcca agtgagcgat cagcttatac tattctcttt 2160 cccttctcat ctttgggcaa catagagcag gggttacaat tcatcaagat agaaacaaga 2220 tgtaaacggt aactggaata taatcaacca atccattggt gtctcaagaa ttgagtacat 2280 gatgcatgta attctttgta agatgacatc cttcttacaa agatgatgag ctttttactt 2340 gttgaggagg gttgtgggtt atccgaataa ccggtttgag agttgttgca cgtacccatg 2400 tttcacaacg ttgtcatcgt tgtatgagta ggtgcgcaac tcattaccca tgacttccta 2460 tctgtggaga gctatcatga atcgcccata atacagaaac actcaagagg ttttgttgca 2520 gctgtccaat gaccaggtct cttttctttg tgcaattgta gttcacagat ggaaagttag 2580 gattgcacta gaagtgtggt tcttctacca aggaacaccc attggatggc acaattgcag 2640 tgggtatcta aattgctgca atgaataata atcgcataga agaccaggca ttctcttttt 2700 aattgcttca agcccattag aaaagttgct cttgttaatc aagttcataa ttcacgtttt 2760 gttactagtc aatgttttca tagataatag cgatttcttt cagaaatata tccaatctag 2820 gaatgtatac atcaccacca tagtttcacc actgccgatt tgccctgctt tttcgatgaa 2880 tgctttgctt aattgtaatc cgcttggata ttgggaatgc aggtaggaga gcccaacgcg 2940 gatcaccgat gctgggagag gccagaagac atggatacac ctcgcaacgt ttacaaagtg 3000 tctacacaga atccaggctc tgatgttgca gcagagacag cagccgcact ggctgctgcc 3060 tccatcgtat ttggtgactc ggacagttcc tactccacca aattacttca cactgcggtg 3120 aaagtaatgc tccctcagaa gtgaattcaa tcgggactca gaaattctct tcattgatct 3180 ttttataagc cactttctct ggaatgcagg tctttgaatt tgcagatcag tacagaggat 3240 cttacagtga ttccctcggt tcagttgtct gcccatttta ctgctcttat tctgggtaca 3300 atgtaagaac tgccattaaa cactagcggc attttctttc gaaaactctc atgtccattg 3360 aactggcttg aaatcaaatt attcctcatg tgaatttgca aaaacaggat gagcttctgt 3420 ggggtgcatc atggcttcat cgggcatcac aaaatgcgtc gtatatgact tacattcagt 3480 cgaatggtca cacactaggg gcagatgatg atgattattc tttcagttgg gatgataaac 3540 gagttggaac caaagttctt ctttccaagg ttttcattct ccacctttct tctagtttct 3600 caaatgcgag agaagctaat aatttacctt ccatggccca cttccacagg gcttcttgca 3660 ggacaggatt gaagaactgc agctatataa agtacatact gataattaca tctgctctct 3720 tatccctgga acatctagtt tccaagccca gtacacacca ggtttgtcgt gtctttgatt 3780 aacaagaaaa gggtccttag cctcatcaaa ctatttctga ggcatctatt ccttacaagt 3840 ggagctcgtg agtctgtgat ctggaccaat gatcttatgg gtccactttg ggcatggcct 3900 aaaataatct cctgggatgg gcattattac ccttgggata caatagaagc ttaagaaaag 3960 gatgcagtaa aggccatcct tcacatggtc gggaagtagg atcttttgac attgagacat 4020 tgtggggcat caaggcgtga tgaccgggat ccatacgatg aatagtgtag accatgaaat 4080 gggttcattt tgcagtatag attcttgttt cctttgaatc aagcattcca taaactattt 4140 atctgcatcg actgacaggt gggcttctct acaaaggaag tgcgagcaat ctacagtatg 4200 taacatccac agcttttctc ctactaacat atgctaatta tctgaactct tccggaggac 4260 atgcttcatg tggtaccact actgttacag ccaagaacct catctcactc gcaaagaaac 4320 aggtacctat atttagtgat atagactatc agcaactcaa ccattcatct ccctgtatgc 4380 tcacagactc gaatggattt attatcaggt tgactacata ttaggtcaga acccggccaa 4440 gatgtcatac atggtaggat tcggagagag gtatccacag catgtccatc acagaggctc 4500 ctcacttcca tctgtacagg tgcaccctaa ttccataccc tgcaatgctg gatttcagta 4560 cctgtattct agcccaccca acccaaatat cctagtgggt gccatattgg gtggtccaga 4620 taacagagac agtttctcag atgaccggaa caattatcag cagtcagaac cagccactta 4680 cataaatgcc ccacttgttg gtgctctagc attcttcgca gcaaacccag tgacggagtg 4740 acagagattg ccctctcttt tacgtaaact gatagaaata ataaactatg gggtttcttt 4800 gtaatttcct atgatggacc tagcagcaaa ccaggtgatg gagtcacaga gattgtcctc 4860 tcttttatgt aaactgttag aaataataaa ctatgggtgt ttctttgtaa tttcctatga 4920 tgggcctagc tgtaatattt gaaagtgtgt ttgaatatat tgagttcagc tctcgattag 4980 ggcctgagct cttttgccct ctctcaatct cacgaatctc tctcaatctc acaaacaaca 5040 tggtatcaga gcattcgatc gtcgcatgaa ttttcatttc t 5081 // |
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