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EBI DbfetchID X58434; SV 1; linear; genomic DNA; STD; PRO; 3361 BP. XX AC X58434; S73625; XX DT 21-JAN-1992 (Rel. 30, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 24) XX DE S.aureus pdhB, pdhC and pdhD genes for pyruvate decarboxylase, DE dihydrolipoamide acetyltransferase and dihydrolipoamide dehydrogenase XX KW dihydrolipoamide acetyltransferase; dihydrolipoamide dehydrogenase; KW lipoamide dehydrogenase; pdh operon; pdhB; pdhC; pdhD; KW pyruvate decarboxylase; pyruvate dehydrogenase (lipoamide); subunit E1beta; KW subunit E2; subunit E3. XX OS Staphylococcus aureus OC Bacteria; Firmicutes; Bacillales; Staphylococcus. XX RN [1] RP 1-3361 RA Hemilae H.; RT ; RL Submitted (13-MAR-1991) to the EMBL/GenBank/DDBJ databases. RL H. Hemilae, Institute of Biotechnology, Valimotie 7, 00380 Helsinki, RL Finland XX RN [2] RP 1-3361 RX PUBMED; 1756171. RA Hemila H.; RT "Lipoamide dehydrogenase of Staphylococcus aureus: nucleotide sequence and RT sequence analysis"; RL Biochim. Biophys. Acta 1129(1):119-123(1991). XX RN [3] RP 1-3361 RX DOI; 10.1016/0923-2508(91)90055-F RX PUBMED; 1784816. RA Hemila H., Palva A., Paulin L., Adler L., Arvidson S., Palva I.; RT "The secretory S complex in Bacillus subtilis is identified as pyruvate RT dehydrogenase"; RL Res. Microbiol. 142(7-8):779-785(1991). XX CC Genes pdhB, pdhC and pdhD of the pdh operon XX FH Key Location/Qualifiers FH FT source 1..3361 FT /organism="Staphylococcus aureus" FT /strain="V8" FT /isolate="ATCC 27733" FT /mol_type="genomic DNA" FT /clone_lib="plasmid pUN101 library in E.coli" FT /clone="plasmid pMBR1" FT /germline FT /db_xref="taxon:1280" FT CDS <1..466 FT /codon_start=2 FT /transl_table=11 FT /gene="pdhB" FT /product="pyruvate dehydrogenase (lipoamide): subunit FT E1beta" FT /EC_number="1.2.4.1" FT /db_xref="GOA:Q59820" FT /db_xref="HSSP:1QS0" FT /db_xref="InterPro:IPR005476" FT /db_xref="UniProtKB/TrEMBL:Q59820" FT /protein_id="CAA41338.1" FT /translation="KEGNDISIITYGAMVQESMKAAEELEKEGNDISNQSHRCKWFKKS FT NESXQKNLXKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVA FT ELSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF" FT RBS 543..548 FT CDS 557..1849 FT /transl_table=11 FT /gene="pdhC" FT /product="dihydrolipoamide acetyltransferase: subunit E2" FT /EC_number="2.3.1.12" FT /db_xref="GOA:Q59821" FT /db_xref="HSSP:2PDE" FT /db_xref="InterPro:IPR004167" FT /db_xref="UniProtKB/Swiss-Prot:Q59821" FT /protein_id="CAA41339.1" FT /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKSV FT VEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKEEA FT PAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDAYLN FT GGAPTASNESADSATNEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVNSKHT FT APHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALNTSFNE FT EAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDGKLTADE FT MKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPVLALSLSF FT DHRQIDGATGQNAMNHIKRLLNNPELLLMEG" FT RBS 1837..1846 FT CDS 1853..3259 FT /transl_table=11 FT /gene="pdhD" FT /product="dihydrolipoamide dehydrogenase: subunit E3" FT /EC_number="1.8.1.4" FT /db_xref="GOA:P0A0E8" FT /db_xref="HSSP:1EBD" FT /db_xref="InterPro:IPR001327" FT /db_xref="UniProtKB/Swiss-Prot:P0A0E8" FT /protein_id="CAA41340.1" FT /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLGG FT VCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGGVE FT GLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFGKRV FT IDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQMTQP FT VKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRPNTDEL FT GLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAEAIDGQA FT AEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLDDTNGFVK FT LITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLGEMTMEAAE FT KAIGYPIHTM" FT terminator 3290..3314 XX SQ Sequence 3361 BP; 1193 A; 535 C; 702 G; 929 T; 2 other; aaaagaaggt aatgacattt caatcataac atacggtgca atggttcaag aatcaatgaa 60 agctgcagaa gaacttgaaa aagaaggtaa tgacatttca aatcaatcac atcggtgcaa 120 atggttcaag aaatcaaatg aaagcncgca gaagaacttg naaaaagatg gttattctgt 180 tgaagtaatt gacttacgta ctgttcaacc aatcgatgtt gatacaattg tagcttcagt 240 tgaaaaaact ggtcgtgcag ttgtagttca agaagcacaa cgtcaagctg gtgttggtgc 300 agcagttgta gctgaattaa gtgaacgtgc aatcctttca ttagaagcac ctattggaag 360 agttgcagca gcagatacaa tttatccatt cactcaagct gaaaatgttt ggttaccaaa 420 caaaaatgac atcatcgaaa aagcaaaaga aactttagaa ttttaataca ttgtaaaagt 480 taacgaagtt agcgtatttt agtctcattg attaaaatga aatgtttaat ttacgaaatc 540 ttaggagggc aaaaacgtgg catttgaatt tagattaccc gatatcgggg aaggtatcca 600 cgaaggtgaa attgtaaaat ggtttgttaa agctggagat actattgaag aagacgatgt 660 tttagctgag gtacaaaacg ataaatcagt agtagaaatc ccatcaccag tatctggtac 720 tgtagaagaa gttatggtag aagaaggtac agtagctgta gttggtgacg ttattgttaa 780 aatcgatgca cctgatgcag aagatatgca atttaaaggt catgatgatg attcatcatc 840 taaagaagaa cctgcgaaag aggaagcgcc agcagagcaa gcacctgtag ctactcaaac 900 tgaagaagta gatgaaaaca gaactgtaaa agcaatgcct tcagtacgta aatacgcacg 960 tgaaaaaggt gttaacatta aagcagtttc tggatctggt aaaaatggtc gtattacaaa 1020 agaagatgta gatgcatact taaatggtgg tgcaccaaca gcttcaaatg aatcagctga 1080 ttcagctaca aatgaagaag ttgctgaaac tcctgcagca cctgcagcag tatcattaga 1140 aggcgacttc ccagaaacaa ctgaaaaaat ccctgctatg cgtagagcaa ttgcgaaagc 1200 aatggttaac tctaagcata ctgcacctca tgtaacatta atggatgaaa ttgatgttca 1260 agcattatgg gatcaccgta agaaatttaa agaaatcgca gctgaacaag gtactaagtt 1320 aacattctta ccatatgttg ttaaagcact tgtttctgca ttgaaaaaat acccagcact 1380 taatacttca ttcaatgaag aagctggtga aattgtgcat aaacattact ggaatatcgg 1440 tattgcggcg gacactgata gaggcttatt agtacctgta gtgaaacatg ctgaccgtaa 1500 atctattttc caaatttctg atgaaattaa cgaattagct gttaaagcac gtgatggtaa 1560 attaacagcc gatgaaatga aaggtgcaac atgcacaatc agtaatatcg gttcagctgg 1620 tggacaatgg ttcactccag ttatcaatca cccagaagta gcaatcttag gaattggccg 1680 tattgctcaa aaacctatcg ttaaagatgg agaaattgtt gcagcaccag tattagcatt 1740 atcattaagc tttgaccaca gacaaattga tggtgcaact ggccaaaatg caatgaatca 1800 cattaaacgc ttattaaata atccagaatt attattaatg gaggggtaaa acatggtagt 1860 tggagatttc ccaattgaaa cagatactat agtaatcgga gcaggtcctg gtggatacgt 1920 tgcagcaatt cgtgcagctc aattaggaca aaaagtaaca atcgttgaga aaggtaatct 1980 tggtggtgtt tgcttaaacg taggatgtat tccttcaaaa gcattactac atgcttctca 2040 ccgttttgtt gaagcacaac attctgaaaa cttaggtgtt attgctgaaa gtgtttcttt 2100 aaacttccaa aaagttcaag aattcaaatc atcagttgtt aataaattaa ctggtggtgt 2160 tgaaggctta cttaaaggta acaaagtaaa catcgttaaa ggtgaagcat atttcgtaga 2220 taacaatagc ttacgtgtta tggacgaaaa gagcgcacaa acatacaact ttaaaaacgc 2280 aatcattgca acaggttcaa gaccaattga aattcctaat ttcaaattcg gtaaacgtgt 2340 tatcgactca acaggtgctt taaatttaca agaagtacca ggtaaattag ttgtagttgg 2400 tggaggatac attggatcag aattaggtac agcatttgct aactttggtt cagaagtaac 2460 catccttgaa ggtgctaaag atatcttagg tggcttcgaa aaacaaatga cacaacctgt 2520 taaaaaaggt atgaaagaaa aaggtgttga aatcgttact gaagcaatgg ctaaatcagc 2580 tgaagaaaca gataacggag ttaaagttac ttatgaagct aaaggcgaag agaaaacaat 2640 cgaagctgat tatgtattag taactgtagg tcgtcgtcca aacacagacg aattaggcct 2700 agaagaatta ggtgttaaat tcgctgaccg tggattatta gaagttgata aacaaagccg 2760 tacgtctatc agcaatatct atgcaattgg tgatatcgtt ccaggtttac cacttgctca 2820 caaagctagc tatgaagcta aagttgctgc tgaagcaatt gatggtcaag ctgctgaagt 2880 tgattacatt ggtatgccag cagtatgctt tactgaacct gaattagcta cagttggtta 2940 ttcagaagcg caagctaaag aagaaggttt agcaattaaa gcttctaaat ttccatatgc 3000 agcaaatggt cgtgcattat cattagacga tactaacgga tttgttaaac ttattacact 3060 taaagaagat gatactttaa tcggtgctca agtagttggt actggtgcat cagatattat 3120 ctctgaatta ggtttagcaa ttgaagctgg tatgaatgct gaagatatcg cattaacaat 3180 ccatgcacat ccaacattag gtgagatgac tatggaagca gctgaaaaag ctatcggata 3240 cccaatccat accatgtaat aactgattat ctataagatt cagtttttaa agctgtagca 3300 tatcctacgg cttttttgtt taggtaataa gtagtgtaag gaaattgatt tgagatatcg 3360 t 3361 // |
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