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EBI DbfetchID X58425; SV 1; linear; genomic DNA; STD; PRO; 2000 BP. XX AC X58425; XX DT 14-JUN-1991 (Rel. 28, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 13) XX DE E.coli (Tn5086) dhfrVII gene for dihydrofolate reductase type VII and sulI DE gene, 5' end XX KW dhfrVII gene; dihydrofolate reductase; dihydropteroate synthase; sulI gene; KW transposon Tn5086; trimethoprim resistance. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OG Plasmid pLM027 XX RN [1] RP 1-2000 RA Sundstroem L.; RT ; RL Submitted (15-MAR-1991) to the EMBL/GenBank/DDBJ databases. RL L. Sundstroem, Dept of Pharmaceutical Microbiology, Uppsala University, RL Biomedical Center, P O Box 581, S-751 23 Uppsala, SWEDEN XX RN [2] RX PUBMED; 8195081. RA Radstrom P., Skold O., Swedberg G., Flensburg J., Roy P.H., Sundstrom L.; RT "Transposon Tn5090 of plasmid R751, which carries an integron, is related RT to Tn7, Mu, and the retroelements"; RL J. Bacteriol. 176(11):3257-3268(1994). XX CC class II transposon Tn5086, integrase-mediated site-specific CC recombination XX FH Key Location/Qualifiers FH FT source 1..2000 FT /organism="Escherichia coli" FT /plasmid="pLM027" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT -35_signal 321..326 FT -10_signal 344..349 FT CDS complement(<1..436) FT /transl_table=11 FT /gene="integrase" FT /db_xref="GOA:P62590" FT /db_xref="InterPro:IPR002104" FT /db_xref="InterPro:IPR004107" FT /db_xref="InterPro:IPR010998" FT /db_xref="InterPro:IPR011010" FT /db_xref="InterPro:IPR011946" FT /db_xref="InterPro:IPR013762" FT /db_xref="UniProtKB/Swiss-Prot:P62590" FT /protein_id="CAA41325.1" FT /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAFI FT RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQEI FT GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGM" FT variation 459 FT /replace="gggg" FT /note="Tn21" FT RBS 581..589 FT CDS 594..1067 FT /transl_table=11 FT /gene="dhfrVII" FT /product="dihydrofolate reductase type VII" FT /note="ttg start codon" FT /db_xref="GOA:P27422" FT /db_xref="InterPro:IPR001796" FT /db_xref="InterPro:IPR012259" FT /db_xref="InterPro:IPR017925" FT /db_xref="UniProtKB/Swiss-Prot:P27422" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA41326.1" FT /translation="MKISLISATSENGVIGNGPDIPWSAKGEQLLFKALTYNQWLLVGR FT KTFDSMGVLPNRKYAVVSRKGISSSNENVLVFPSIEIALQELSKITDHLYVSGGGQIYN FT SLIEKADIIHLSTVHVEVEGDINFPKIPENFNLVFEQFFLSNINYTYQIWKKG" FT repeat_region 1108..1171 FT /rpt_type=INVERTED FT /rpt_unit_range=1108..1136 FT /rpt_unit_range=1144..1171 FT CDS 1298..1645 FT /transl_table=11 FT /note="ORF 1" FT /db_xref="GOA:P0AA22" FT /db_xref="InterPro:IPR000390" FT /db_xref="UniProtKB/Swiss-Prot:P0AA22" FT /protein_id="CAA41327.1" FT /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY FT FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP FT SWKSLRRPTPW" FT CDS 1639..>2000 FT /transl_table=11 FT /gene="sulI" FT /product="dihydropteroate synthase" FT /db_xref="GOA:P0C002" FT /db_xref="InterPro:IPR000489" FT /db_xref="InterPro:IPR006390" FT /db_xref="InterPro:IPR011005" FT /db_xref="UniProtKB/Swiss-Prot:P0C002" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA41328.1" FT /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVG FT PAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQ FT GFPDPALYPDIAEADC" XX SQ Sequence 2000 BP; 492 A; 491 C; 501 G; 516 T; 0 other; gcatgcccgt tccatacaga agctgggcga acaaacgatg ctcgccttcc agaaaaccga 60 ggatgcgaac cacttcatcc ggggtcagca ccaccggcaa gcgccgcgac ggccgaggtc 120 ttccgatctc ctgaagccag ggcagatccg tgcacagcac cttgccgtag aagaacagca 180 aggccgccaa tgcctgacga tgcgtggaga ccgaaacctt gcgctcgttc gccagccagg 240 acagaaatgc ctcgacttcg ctgctgccca aggttgccgg gtgacgcaca ccgtggaaac 300 ggatgaaggc acgaacccag tggacataag cctgttcggt tcgtaagctg taatgcaagt 360 agcgtatgcg ctcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg 420 cagtggcggt tttcatggct tgttatgact gtttttttgt acagtctatg cctcgggcat 480 ccaagcagca agcgcgttac gccgtgggtc gatgtttgat gttatggagc agcaacgatg 540 ttacgcagca gggcagtcgc cctaaaacaa agttagccat tacgggggtt gaattgaaaa 600 tttcattgat ttctgcaacg tcagaaaatg gcgtaatcgg taatggccct gatatcccat 660 ggtcagcaaa aggtgagcag ttactcttta aagcgctcac atataatcag tggctccttg 720 ttggaaggaa aacatttgac tctatgggtg ttcttccaaa tcgaaaatat gcagtagtgt 780 cgaggaaagg aatttcaagc tcaaatgaaa atgtattagt ctttccttca atagaaatcg 840 ctttgcaaga actatcgaaa attacagatc atttatatgt ctctggtggc ggtcaaatct 900 acaatagtct tattgaaaaa gcagatataa ttcatttgtc tactgttcac gttgaggttg 960 aaggtgatat caattttcct aaaattccag agaatttcaa tttggttttt gagcagtttt 1020 ttttgtctaa tataaattac acatatcaga tttggaaaaa aggctaacaa gtcgttccag 1080 caccagtcgc tgcgctcctt ggacagtttt ttaagtcgcg gttttatggt tttgctgcgc 1140 aaaagtattc cataaaacca caacttaaaa accgccgctg aactcggcgt tagatgcact 1200 aagcacataa ttgctcacag ccaaactatc aggtcaagtc tgcttttatt atttttaagc 1260 gtgcataata agccctacac aaattgggag atatatcatg aaaggctggc tttttcttgt 1320 tatcgcaata gttggcgaag taatcgcaac atccgcatta aaatctagcg agggctttac 1380 taagcttgcc ccttccgccg ttgtcataat cggttatggc atcgcatttt attttctttc 1440 tctggttctg aaatccatcc ctgtcggtgt tgcttatgca gtctggtcgg gactcggcgt 1500 cgtcataatt acagccattg cctggttgct tcatgggcaa aagcttgatg cgtggggctt 1560 tgtaggtatg gggctcataa ttgctgcctt tttgctcgcc cgatccccat cgtggaagtc 1620 gctgcggagg ccgacgccat ggtgacggtg ttcggcattc tgaatctcac cgaggactcc 1680 ttcttcgatg agagccggcg gctagacccc gccggcgctg tcaccgcggc gatcgaaatg 1740 ctgcgagtcg gatcagacgt cgtggatgtc ggaccggccg ccagccatcc ggacgcgagg 1800 cctgtatcgc cggccgatga gatcagacgt attgcgccgc tcttagacgc cctgtccgat 1860 cagatgcacc gtgtttcaat cgacagcttc caaccggaaa cccagcgcta tgcgctcaag 1920 cgcggcgtgg gctacctgaa cgatatccaa ggatttcctg accctgcgct ctatcccgat 1980 attgctgagg cggactgcag 2000 // |
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