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EBI DbfetchID X57401; SV 1; linear; genomic DNA; STD; PRO; 4621 BP. XX AC X57401; XX DT 27-JUN-1991 (Rel. 28, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 6) XX DE K.pneumoniae scrY, scrA and scrB genes for sucrose porin, sucrose-specific DE enzyme II and sucrose-6-phosphate hydrolase, resp. XX KW integral cytoplasmic membrane protein; scrA gene; scrB gene; scrY gene; KW sucrose porin; sucrose transport; sucrose-6-phosphate hydrolase; KW sucrose-specific enzyme II. XX OS Klebsiella pneumoniae OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Klebsiella. XX RN [1] RP 1-4621 RA Jahreis K.; RT ; RL Submitted (28-JAN-1991) to the EMBL/GenBank/DDBJ databases. RL K. Jahreis, Abt Genetik FB 5 Biologie, Chemie Universitaet Osnabrueck, RL Barbarastr 11, 4500 Osnabrueck XX RN [3] RP 35-1744 RX DOI; 10.1111/j.1365-2958.1991.tb00769.x RX PUBMED; 1649946. RA Schmid K., Ebner R., Jahreis K., Lengeler J.W., Titgemeyer F.; RT "A sugar-specific porin, ScrY, is involved in sucrose uptake in enteric RT bacteria"; RL Mol. Microbiol. 5(4):941-950(1991). XX RN [4] RP 1-4621 RX DOI; 10.1007/s004380050067 RX PUBMED; 8628219. RA Titgemeyer F., Jahreis K., Ebner R., Lengeler J.W.; RT "Molecular analysis of the scrA and scrB genes from Klebsiella pneumoniae RT and plasmid pUR400, which encode the sucrose transport protein Enzyme II RT Scr of the phosphotransferase system and a sucrose-6-phosphate invertase"; RL Mol. Gen. Genet. 250(2):197-206(1996). XX CC See also X57400. XX FH Key Location/Qualifiers FH FT source 1..4621 FT /organism="Klebsiella pneumoniae" FT /map="65'" FT /strain="1033-5P14" FT /mol_type="genomic DNA" FT /clone="KAY 2026" FT /db_xref="taxon:573" FT misc_binding 28..49 FT /bound_moiety="CAP" FT /note="putative" FT misc_binding 52..63 FT /bound_moiety="Scr repressor" FT /note="putative" FT /experiment="experimental evidence, no additional details FT recorded" FT misc_binding 109..122 FT /bound_moiety="Scr repressor" FT /note="putative" FT /experiment="experimental evidence, no additional details FT recorded" FT -10_signal 130..135 FT /note="putative" FT RBS 154..159 FT /note="putative" FT CDS 167..1684 FT /transl_table=11 FT /gene="scrY" FT /product="sucrose porin" FT /db_xref="GOA:P27218" FT /db_xref="InterPro:IPR003192" FT /db_xref="UniProtKB/Swiss-Prot:P27218" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA40657.1" FT /translation="MYKKRKLAILIALLTGTAAAHGQTDLNSIEARLAALEKRLQDAET FT RASTAESRAASAEQKVQQLTQQQQQTQATTQQVARRTTQLEEKAERPGGFEFHGYARSG FT VIMNDSAASTKSGAYMTPAGETGGAIGRLGNQADTYVEMNLEHKQTLDNGATTRFKVMV FT ADGQTTYNDWTASSSDLNVRQAFVELGNLPTFEGPFKGSTLWAGKRFDRDNFDIHWIDS FT DVVFLAGTGGGIYDVKWNDSLRSNFSLYGRNFGDIADSSNSVQNYIVSMNNFAGPVQMM FT VSGMRAKDNDDRQDANGNLVKGDAANTGVHALLGLHNESFYGLRDGTSKTALLYGHGLG FT AEVKGIGSDGALRPGANTWRFASYGTTPLSDRWFIAPAVLAQSSKDRYVDGDSYQWATL FT NLRLIQEVTQNFALAWEGSYQYMDLQPEGYNDRHAVNGSFYKLTFAPTFKVGSIGDFFS FT RPEIRFYTSWMDWSKKLDNYANDDALGSNGFKSGGEWSFGMQMETWF" FT CDS 1789..3159 FT /transl_table=11 FT /gene="scrA" FT /product="sucrose-specific enzyme II" FT /db_xref="GOA:P27219" FT /db_xref="InterPro:IPR001996" FT /db_xref="InterPro:IPR003352" FT /db_xref="InterPro:IPR004719" FT /db_xref="InterPro:IPR010973" FT /db_xref="InterPro:IPR011535" FT /db_xref="InterPro:IPR013013" FT /db_xref="InterPro:IPR018113" FT /db_xref="UniProtKB/Swiss-Prot:P27219" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA40658.1" FT /translation="MDFEQISRSLLPLLGGKENIASAAHCATRLRLVLVDDALADQQAI FT GKIDGVKGCFRNAGQMQIIFGTGVVNKVYAAFIQAAGISESSKSEAADLAAKKLNPFQR FT IARLLSNIFVPIIPAIVASGLLMGLLGMVKTYGWVDPSNALYIMLDMCSSAAFIILPIL FT IGFTAAREFGGNPYLGATLGGILTHPALTNAWGVAAGFHTMNFFGIEVAMIGYQGTVFP FT VLLAVWFMSMVEKRLRRVIPDALDLILTPFLTVIISGFIALLLIGPAGRALGDGISFIL FT STLISHAGWLAGLLFGGLYSVIVITGIHHSFHAIEAGLLGNPSIGVNFLLPIWAMANVA FT QGGACFAVWFKTKDAKIKAITLPSAFSAMLGITEAAIFGINLRFVKPFIAALVGGAAGG FT AWVVSMHVYMTAVGLTAIPGMAIVQASSLLNYIIGMAIAFAVAFALSLTLKYKTDAE" FT RBS 3147..3151 FT /note="putative" FT CDS 3159..4559 FT /transl_table=11 FT /gene="scrB" FT /product="beta-fructofuranosidase" FT /product="sucrose-6-phosphate hydrolase" FT /EC_number="3.2.1.26" FT /db_xref="GOA:P27217" FT /db_xref="InterPro:IPR001362" FT /db_xref="InterPro:IPR006232" FT /db_xref="InterPro:IPR008985" FT /db_xref="InterPro:IPR013148" FT /db_xref="InterPro:IPR013189" FT /db_xref="InterPro:IPR018053" FT /db_xref="UniProtKB/Swiss-Prot:P27217" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA40659.1" FT /translation="MSLPSRLPAILQAVMQGQPQALADSHYPQWHLAPVNGLLNDPNGF FT CQVAGRYHLFYQWNPLACDHTYKCWGHWSSADLLHWRHEPIALMPDEEYDRNGCYSGSA FT VEFEGALTLCYTGNVKFPDGGRTAWQCLATENADGTFRKLGPVLPLPEGYTGHVRDPKV FT WRQDGRWYMVLGAQDVQQRGKVLLFTASDLREWRLVGEIAGHDVNGLANAGYMWECPDL FT FPLADTHLLICCPQGLAREAQRFLNTYPAVWMAGRFDAERGIFDHGPLHELDSGFEFYA FT PQTMQADDGRRLLVGWMGVPDGDEMHQPTRAQGWIHQMTCVRELEWQAGTLYQRPLREL FT VALRGEAQGWCGQTLPLAPMELAFDLSPDSTLGLDFAGALQLTVNRDGLRLSRRGLQTA FT EMHHRYWRGEARRLRIFIDRSSVEIFINDGEGVMSSRFFPGYPGQLIFSGATPVAFCRW FT LLRPCMVE" XX SQ Sequence 4621 BP; 899 A; 1432 C; 1401 G; 889 T; 0 other; gatccccttg ggccggttaa cggcccactt tgctggcgac atcacaattc ttaaaccggt 60 ttagcaattt ttattttcac cgcgttaccg acatgtttac catatcaact aaaccggttt 120 agcaaacatt agcacactca ctgatttacc tttggatgtc accaacatgt ataaaaaacg 180 gaagttagcc attcttattg ctttgctaac cggcaccgcc gccgcccatg ggcagacaga 240 cctgaacagc attgaagcgc gtctcgccgc cctggaaaaa cgcctgcagg acgccgagac 300 ccgcgccagc actgccgaaa gccgcgccgc ctcagcggag cagaaagttc agcagttaac 360 ccagcagcag cagcaaaccc aggccaccac ccagcaggtg gccaggcgca ccactcaact 420 ggaagaaaaa gccgaacggc ccggcggctt tgagttccat ggctatgcgc gttccggggt 480 gatcatgaac gactcggccg ccagtaccaa atccggcgct tatatgaccc ccgccgggga 540 gaccggcggc gccattggtc gcctgggcaa ccaggccgac acctatgtgg aaatgaacct 600 cgaacataaa cagaccctgg acaacggggc gaccacccgt ttcaaagtga tggtggccga 660 cggacagacc acctataacg actggacggc aagcagcagc gacctgaacg tacgccaggc 720 gttcgtcgag ctgggcaatc tgccgacctt cgaaggcccg ttcaaaggct cgaccctgtg 780 ggccgggaaa cgctttgacc gcgacaactt cgacatccac tggattgact cggatgtggt 840 gttcctcgcc gggaccggcg gcgggatcta cgacgtgaaa tggaacgaca gcctgcgcag 900 caacttctcg ttatacggcc gcaactttgg cgatatcgcc gacagcagca acagcgtgca 960 gaactatatc gtcagcatga ataactttgc cggcccggtg cagatgatgg tcagcgggat 1020 gcgggcgaaa gataatgacg accgccagga cgcgaacggc aatctggtga aaggcgatgc 1080 cgctaacacc ggggttcatg ccctgctggg cctgcacaat gagagcttct atggcctgcg 1140 cgacgggacc agcaaaacgg ccctgctgta cggccacggg ctgggcgccg aggttaaagg 1200 catcggctcc gacggcgcgc tgcgcccggg ggccaatacc tggcgcttcg ccagctatgg 1260 cactacgccg ctgagcgatc gctggtttat tgccccggcc gtgctggcgc agagcagtaa 1320 agatcgttat gtcgatggcg acagctatca gtgggccacc ctcaacctgc gtctgattca 1380 ggaagtgacg cagaacttcg ccctcgcctg ggagggcagc tatcagtaca tggatctgca 1440 gcctgaaggc tacaacgatc gccatgcggt caatggcagc ttctacaagc tgaccttcgc 1500 cccgaccttc aaggtgggca gcatcggcga cttcttctcg cggccggaga tccgcttcta 1560 tacatcgtgg atggactgga gcaaaaaact ggacaactac gccaacgatg acgcgttagg 1620 cagcaacgga ttcaaatcgg gcggcgaatg gtcgttcggt atgcaaatgg agacctggtt 1680 ctgacggcca ccggggcgac agggtaaata acacataaat ataaggttcg cggcgcctgc 1740 cacggctggc gccgcccacg ccatatcatc atgcatttag agggtactat ggattttgaa 1800 cagatttccc gctcactgct tcccctgctg ggcggcaagg aaaatatcgc cagcgccgcg 1860 cactgcgcca cccgcctgcg gctggtgctg gtcgacgacg cgctcgccga tcagcaggcg 1920 attggcaaaa tcgacggggt gaaaggctgc tttcgcaatg ccggacagat gcagatcatc 1980 ttcggcaccg gggtggtcaa taaagtctat gccgccttta tccaggccgc aggcatcagc 2040 gaatcgagca aatccgaagc cgccgacctg gcggcgaaaa agctgaaccc gttccagcgc 2100 atcgcccgcc tgctgtccaa catcttcgtg ccgattattc cggccatcgt cgcctccggc 2160 ctgctgatgg gcctgctggg gatggtgaaa acctacggtt gggtcgaccc gagcaacgct 2220 ctctatatca tgctggatat gtgcagttcg gcggcgttta tcattctgcc gatcctgatc 2280 ggctttaccg ccgcccgcga atttggcggt aacccctatc tgggcgcgac cctcggcggg 2340 atcctcaccc acccggcgct gaccaacgcc tggggcgtcg ccgccggctt ccacaccatg 2400 aatttcttcg gcatcgaagt ggcgatgatc ggctaccagg gcaccgtctt cccggtgctg 2460 ctggcggtgt ggtttatgag catggtcgag aagcggctgc gccgcgtgat ccctgacgcg 2520 ctggacctga tcctcactcc gttcctgacg gtgattatct ccggctttat cgccctgctg 2580 ctgatcggcc cggccggtcg cgcgctcggc gacggcattt cgtttatcct cagcacgctt 2640 atcagccatg ccggctggct ggcgggcctg ctgttcggcg gcctctattc ggtgatcgtc 2700 attaccggta tccatcacag cttccatgcc atcgaggccg gactgctggg caacccatcg 2760 attggcgtca acttcctgct gccgatctgg gcgatggcca acgtcgccca gggcggcgcc 2820 tgctttgcgg tgtggtttaa aaccaaagat gccaaaataa aagccatcac cctgccgtcg 2880 gcgttttcgg cgatgctggg gatcaccgag gcggcaatct tcgggattaa cctgcgcttt 2940 gtgaaaccgt ttatcgccgc gctggtgggc ggtgccgccg gcggcgcctg ggtggtgtcg 3000 atgcacgtct acatgaccgc ggtgggcctg acggcgatcc cgggaatggc tatcgtgcag 3060 gccagctcgc tgctgaacta cattatcgga atggcgatcg ccttcgccgt ggccttcgcg 3120 ctctctctga cgctgaaata caaaacggac gctgaataat gtcattaccg tcacgtctgc 3180 ctgcgatcct gcaggccgtt atgcagggcc agccgcaggc gctggccgac agccattatc 3240 cgcaatggca tctggcgccg gtcaacggac tgctgaacga tcctaacggc ttttgccagg 3300 tcgccgggcg ttaccacctg ttttatcagt ggaacccgct cgcctgcgac catacctata 3360 agtgctgggg acactggagc tctgccgatc tgctgcactg gcggcacgaa cctatcgccc 3420 tgatgccgga tgaagagtat gaccgcaacg gctgctactc tggcagcgcg gtcgagttcg 3480 agggtgccct gactctgtgc tacaccggca acgtgaaatt ccccgacggc gggcgcaccg 3540 cctggcaatg tctggcgacc gagaatgccg atggcacctt ccgcaagctg gggccggtgc 3600 tgccgctgcc agaaggctat accggccatg tgcgcgaccc taaagtgtgg cggcaggacg 3660 ggcgctggta catggttctt ggggcgcagg atgtgcaaca gcgcggcaaa gtgctgctgt 3720 ttaccgccag cgacctgcgg gagtggcgcc tggtgggcga gatcgccggg cacgacgtga 3780 acggcctggc gaacgccggc tacatgtggg agtgcccgga tctctttccg ctggcggaca 3840 cccacctgct gatctgctgc ccgcaggggc tggcccgcga agcgcagcgc tttctcaata 3900 cctatccggc ggtgtggatg gcaggccgct tcgacgccga acgcgggatc ttcgaccacg 3960 gcccgctgca cgagctggac agcggatttg agttctacgc gccgcagacc atgcaggccg 4020 acgatggccg ccgcctgctg gtcggctgga tgggcgtccc cgacggggac gagatgcatc 4080 agcccacccg cgcgcagggc tggatccatc agatgacctg cgtgcgtgag ctggagtggc 4140 aggctggcac tctgtatcag cgtccgctgc gcgagctggt cgccctgcgc ggggaagccc 4200 agggctggtg cggacagacc ctgcccctcg ccccgatgga gctggccttt gacctttccc 4260 ccgacagcac gctggggctg gactttgccg gcgccctgca gctcaccgtc aatcgcgacg 4320 gcctacgtct gtcgcgccgc ggcctgcaga cggcagagat gcatcaccgc tactggcgcg 4380 gcgaggcgcg acgcctgcgg atctttatcg accgctccag cgtggagatt ttcatcaacg 4440 atggcgaggg ggtgatgagc agccgcttct ttccgggcta tccggggcag ctcatcttca 4500 gcggtgcgac gccggtggca ttctgccgct ggctgctgcg gccatgcatg gtagaataag 4560 cgttttgctt tcaggctccc gggtgctgat gaaaaccaaa cgcgtaacta tcaaagatat 4620 c 4621 // |
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