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EBI DbfetchID X56977; SV 1; linear; genomic DNA; STD; PRO; 2608 BP. XX AC X56977; XX DT 19-MAR-1991 (Rel. 27, Created) DT 19-MAR-1991 (Rel. 27, Last updated, Version 2) XX DE M. bovis mboIIM and mboIIR genes coding respectively for methyltransferase DE MboII and endodeoxyribonuclease MboII XX KW endodeoxyribonuclease; mboIIM gene; mboIIR gene; methyltransferase. XX OS Moraxella bovis OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Moraxellaceae; Moraxella. XX RN [1] RP 1-2608 RA Bocklage H.; RT ; RL Submitted (13-DEC-1990) to the EMBL/GenBank/DDBJ databases. RL H. Bocklage, INSTITUT FUER GENETIK, UNIVERSUITAET ZU KOELN, WEYERTAL 121, RL D-5000 KOELN 41, F R G XX RN [2] RP 1-2608 RX DOI; 10.1093/nar/19.5.1007 RX PUBMED; 2020540. RA Bocklage H., Heeger K., Mueller-Hill B.; RT "Cloning and characterization of the MboII restriction-modification RT system"; RL Nucleic Acids Res. 19(5):1007-1013(1991). XX FH Key Location/Qualifiers FH FT source 1..2608 FT /organism="Moraxella bovis" FT /strain="ATCC 10900" FT /mol_type="genomic DNA" FT /clone_lib="pUC9" FT /db_xref="taxon:476" FT RBS 100..103 FT /note="methylase RBS" FT CDS 112..894 FT /transl_table=11 FT /gene="mboIIM" FT /product="methyltransferase MboII" FT /db_xref="GOA:P23192" FT /db_xref="InterPro:IPR002052" FT /db_xref="PDB:1G60" FT /db_xref="UniProtKB/Swiss-Prot:P23192" FT /protein_id="CAA40297.1" FT /translation="MLEINKIHQMNCFDFLDQVENKSVQLAVIDPPYNLSKADWDSFDS FT HNEFLPFTYRWIDKVLDKLDKDGSLYIFNTPFNCAFICQYLVSKGMIFQNWITWDKRDG FT MGSAKRGFSTGQETILFFSKSKNHTFNYDEVRVPYESTDRIKHASEKGILKNGKRWFPN FT PNGRLCGEVWHFSSQRHKEKVNGKTVKLTHITPKPRDLIERIIRASSNPNDLVLDCFMG FT SGTTAIVAKKLGRNFIGCDMNAEYVNQANFVLNQLEIN" FT RBS 892..896 FT /note="restrictase RBS" FT CDS 903..2153 FT /transl_table=11 FT /gene="mboIIR" FT /product="endodeoxyribonuclease MboII" FT /db_xref="GOA:P23191" FT /db_xref="UniProtKB/Swiss-Prot:P23191" FT /protein_id="CAA40298.1" FT /translation="MKNYVSNINLGNSSLKFIDERLQSENYRGIHLSQHNRYDLPKLID FT ILTLLNKHAPNQSLMQIRTTDISKRPQNIPEEQSYAEFCNEAKSLTNIGTQDAMRKNLF FT VDFARMGLINRYNDKKVLTDPFKRGVTKYVALSDMGVKLIDPKLDILSKNLIFSKSLNK FT LLTGFVEDVLSLLTNSDLKEISFDEFMLFVSAMNCNFNFSISTEQCESLIKEYRLLSRV FT QKNAVIDTLKSELIPDNFNGDKKDKRDYHNWANENQQIWTLFENIPFFIMEKDSRKLIL FT ITSDVDLSKYSKSKMKRSQQAKNDYFKHHKVNKIKGYELDHIIPLLEAESVDEYRYLDN FT WLNLLYIDGKTHAIKSQSGSKYYIFTFDDNDYNQIYFLDTQGDKLSINNDDTALFDKNK FT VPKIYEYNQNFINAKTS" XX SQ Sequence 2608 BP; 959 A; 448 C; 456 G; 745 T; 0 other; aaaatcaatt agaatatatg gaaaacagaa acaattgtta tgcaatttcc gtaagagatt 60 taaaccaaat aggtacacta atagatatta tttaccaaaa tggttgaaaa tatgctagaa 120 ataaataaaa tacatcaaat gaactgcttt gattttttag accaagtgga aaataaaagc 180 gttcaattag ccgtcattga cccgccttac aatcttagta aggcggattg ggatagtttt 240 gatagccata atgaattttt gccatttact tatcgttgga ttgataaagt tttagataaa 300 ctggataaag acggctcgct ttatattttt aatacaccat ttaattgtgc ttttatttgt 360 caatacttgg taagtaaagg tatgattttt caaaattgga ttacttggga taaaagagat 420 ggaatgggta gtgccaaaag gggtttttcc acaggacaag aaaccatttt atttttttca 480 aaatccaaaa atcacacttt taattatgac gaagtgcgag tgccttatga aagcaccgac 540 agaataaagc acgccagtga aaaaggcatt ttaaaaaatg gtaaacgctg gtttcccaat 600 cctaatggca ggttatgtgg tgaagtttgg catttttcta gccaacgcca caaagaaaaa 660 gtcaatggca aaaccgtcaa attgacccat attactccaa aaccacgcga tttaatagaa 720 cgcattatta gagcaagttc caaccccaac gatttggtat tagattgttt tatgggaagt 780 ggcacaacag caatcgttgc caaaaaactg ggtcgtaatt ttattgggtg cgatatgaat 840 gctgaatatg ttaatcaagc caattttgtg ttaaatcaac ttgaaatcaa ttaaggaaaa 900 ctatgaaaaa ttatgtcagc aacatcaatc ttggtaattc atcattaaaa ttcattgatg 960 aaagattgca atctgaaaat tatcgtggca ttcatttaag tcagcataac cgctatgatt 1020 tgccaaaatt aatagatatt ttgacattgt taaataaaca cgcccccaac caatctttaa 1080 tgcaaatcag aacaactgac atcagcaaaa gaccgcagaa tatcccagaa gagcaatctt 1140 atgccgaatt ttgcaatgaa gccaaaagtt taaccaatat tggcacgcaa gatgcaatgc 1200 gtaaaaatct ttttgtggat tttgcaagaa tgggactaat caaccgatat aacgacaaaa 1260 aagtattaac tgacccattc aaaaggggcg taacaaaata tgttgctttg tccgatatgg 1320 gagtaaagtt aatagaccca aaattagata tattaagtaa aaatttaatt ttttctaagt 1380 cgttaaataa actattaaca ggctttgtgg aagatgtttt aagtttgcta accaatagtg 1440 atttaaaaga aatcagcttt gatgagttta tgctgtttgt ttcagcaatg aattgtaatt 1500 ttaattttag catttccaca gagcaatgcg aaagtttgat aaaagaatac cgattattat 1560 cacgagtaca aaaaaatgcg gtgattgaca ctttaaaatc agagcttatt ccagacaatt 1620 ttaacggcga taaaaaagat aagcgagatt atcataattg ggcaaatgaa aatcaacaaa 1680 tatggacttt gtttgaaaat attccatttt tcattatgga aaaagacagt agaaaactta 1740 ttttaataac ttctgatgta gatttgtcaa aatacagtaa atcaaaaatg aaacgctcac 1800 aacaagccaa aaatgattat tttaaacacc acaaagtcaa taaaataaaa ggctatgaat 1860 tagaccacat cattccttta ttggaagcag aaagcgtgga tgaatatcgt tatttagata 1920 actggcttaa tttattgtac attgatggca aaacacacgc cattaaaagt caaagcggtt 1980 caaaatatta tatctttaca tttgatgaca atgattataa tcagatttat tttttggaca 2040 cccaaggcga taaactgtca attaacaatg acgatactgc tttatttgac aagaataaag 2100 taccaaaaat ttatgaatac aatcaaaact ttatcaacgc caaaacatca taattatttt 2160 taaggaaacc caatgaccac cctacccgac tatgtccgtg ccattattac cgcaaccgtt 2220 tatgatgtcg ccattcgcac accgcttgat aaggcaagca aactctctgc ccgttttggc 2280 aacgacattc gcctaaaacg agaagacctg caacctgtat ttagctttaa agtgcgtggg 2340 gcgtacaaca aaatcagcca actttcggca gagcaaaaag caaaaggcat catctgtgcc 2400 tcggctggca accacgcaca aggggtagcg tatcggcaaa tcgcttaaaa atcaataact 2460 taattgtaat gcccaaaacc acgcccgaca tcaaagtgca agcggtcaaa gcctttggtg 2520 gtacggtgca tttgcatggt gacagttttg acgaagccaa ccgctatgcc atggaaaagg 2580 caacaacgga cggtatgacc tactcgcc 2608 // |
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