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EBI DbfetchID X56656; SV 1; linear; genomic DNA; STD; PRO; 2292 BP. XX AC X56656; S44834; XX DT 23-FEB-1993 (Rel. 35, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 4) XX DE L.enzymogenes phoA gene for secreted alkaline phosphatase XX KW alkaline phosphatase; phoA gene; secreted protein. XX OS Lysobacter enzymogenes OC Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; OC Xanthomonadaceae; Lysobacter. XX RN [1] RA Roy K.L.; RT ; RL Submitted (22-NOV-1990) to the EMBL/GenBank/DDBJ databases. RL K.L. Roy, UNIVERAITY OF ALBERTA, DEPT OF MICROBIOLOGY, M-330 BIOLOGICAL RL SCIENCES BLDG, EDMONTON ALBERTA T6G 2E9, CANADA XX RN [2] RP 1-2292 RX PUBMED; 1856159. RA Au S., Roy K.L., von Tigerstrom R.G.; RT "Nucleotide sequence and characterization of the gene for secreted alkaline RT phosphatase from Lysobacter enzymogenes"; RL J. Bacteriol. 173(15):4551-4557(1991). XX RN [3] RA Roy K.L.; RT ; RL Submitted (20-NOV-1990) to the EMBL/GenBank/DDBJ databases. RL Roy K.L., University of Alberta, Department of Microbiology, M-330 RL Biological Sciences Building, Edmonton, Alberta, Canada, T6G 2E9. XX FH Key Location/Qualifiers FH FT source 1..2292 FT /organism="Lysobacter enzymogenes" FT /strain="ATCC 29487" FT /mol_type="genomic DNA" FT /clone_lib="genomic DNA library in lambda" FT /sub_clone="pUC118" FT /db_xref="taxon:69" FT prim_transcript 263..>2292 FT /gene="phoA" FT RBS 278..282 FT sig_peptide 289..1086 FT /gene="phoA" FT CDS 289..1908 FT /transl_table=11 FT /gene="phoA" FT /product="alkaline phosphatase" FT /note="secreted" FT /db_xref="GOA:Q05205" FT /db_xref="InterPro:IPR004843" FT /db_xref="UniProtKB/Swiss-Prot:Q05205" FT /protein_id="CAA39978.1" FT /translation="MNLSPSRTPICAALAAALLGAAALAPAHAAQRILQLSEDTTHSKP FT VSAASALRGTPLAKAGAADRVCEAGAKWLRVGFKQLKLAGYDSLVLTSSGGDKLVFEGQ FT HWNQRSFTTRPLRGECVDIQPYFSQPDSAFQLDRYDYSTVALDKATVVVAGAGDICDTS FT GNACQGTSDLIVSINPTAVFTAGDNAYNSGTLSEYNSRYAPTWGRFKALTSPSPGNHDY FT STTGAKGYFDYFNGSGNQTGPAGDRSKGYYSWDVGDWHFVSLNTMSGGTVAQAQIDWLK FT ADLAANTKPCTAAYFHHPLLSRGSYSGYSQVKPFWDALYAAKADLVLVGHDHNYQRYGK FT MNPDKAAASDGIRQVLVGTGGRAFYGISGSHALLEASNDSTFGVLKLTLSATGYTGDFV FT PRAGSSYTDHFTGTCNKGSGNPPTQTLTLNSVRDVTVKSGGSRDNGATLYADGSDGGQV FT LRGLMAWNVSSAAGKTLTGAQVKLQVSDRSTGTYDLYRAGAAWTEANASYSGVSLGSKI FT GSVVPSATGAQSIALNAAGFSW" FT mat_peptide 1087..1905 FT /gene="phoA" FT /product="alkaline phosphatase" FT /note="secreted" FT repeat_region 1976..1981 FT /rpt_type=INVERTED FT old_sequence 1996..2011 FT /replace="cgccaatgcgccgca" FT /citation=[2] FT repeat_region 1998..2003 FT /rpt_type=INVERTED FT old_sequence 2046..2047 FT /replace="tcg" FT /citation=[2] FT repeat_region 2080..2093 FT /rpt_type=INVERTED FT repeat_region 2102..2115 FT /rpt_type=INVERTED XX SQ Sequence 2292 BP; 388 A; 851 C; 730 G; 323 T; 0 other; tgatatcgat aagcatgcgt tctttacaca cgaaacgtga ggccgcaggc cgcgcccgct 60 tgtgcgcctc gcgcgtgggc gcgcccgtct gcggcggtga tgcgagcgct gcggcgaaga 120 caagtcggaa cttgtgacgg acgccgcgct gacggcgcaa cagccgcgca ttgcgtcgca 180 atcgtgtagc agttttccgg ccaaactccg cagccatcgc ggccttgccc ggccgcgatg 240 cccgccttca tccgccccgc taccagccac tcccgccgga gacagccagt gaacctctcg 300 ccctcgcgca cgccgatctg cgccgccctc gccgccgccc tgctcggcgc cgccgcgctc 360 gcgcccgcgc acgccgccca gcgcatcctg cagctgtcgg aggacaccac ccacagcaag 420 ccggtcagcg ccgcctccgc gttgcgcggc acgccgctgg ccaaggccgg cgccgcggat 480 cgcgtgtgcg aagccggggc caagtggcta cgcgtgggct tcaagcagct caagctggcc 540 ggctacgact cgctggtgct gaccagcagc ggcggcgaca agctcgtgtt cgaaggccag 600 cactggaacc agcgcagctt caccacccgc ccgctgcgcg gcgagtgcgt cgacatccag 660 ccgtatttca gccagcccga cagcgccttc cagctcgacc gctacgacta cagcacggtc 720 gcgctggaca aggccacggt ggtcgtggcc ggcgccggcg acatctgcga caccagcggc 780 aacgcctgcc agggcacctc ggacctgatc gtctcgatca accctaccgc ggtgttcacc 840 gccggcgaca acgcctacaa cagcggcacg ctgagcgaat acaacagccg ctacgcgccg 900 acctggggcc ggttcaaggc gctgaccagc ccgtcgccgg gcaaccacga ctacagcacc 960 accggcgcca agggctactt cgactacttc aacggcagcg gcaaccagac cggcccggcc 1020 ggcgaccgca gcaagggcta ctacagctgg gacgtcggcg actggcactt cgtctccctc 1080 aacaccatga gcggcggcac cgttgcgcaa gcccagatcg attggctcaa ggccgacctc 1140 gccgccaaca ccaagccctg caccgcggcc tatttccatc atccgctgct cagccgcggc 1200 agctactcgg gctacagcca ggtcaagccg ttctgggacg cgctttacgc ggccaaggcc 1260 gacctggtgc tggtcggcca cgaccacaac taccagcgct acggcaagat gaatcccgac 1320 aaggccgcgg ccagcgacgg catccgccag gtgttggtcg gcaccggcgg ccgcgccttc 1380 tacggcatca gcggcagcca cgcgctgctg gaagccagca acgacagcac cttcggcgtg 1440 ctcaagctga ccttgagcgc gaccggctac accggcgact tcgtgccgcg cgccggcagc 1500 agctacaccg accatttcac cggcacctgc aacaagggca gcggcaaccc gccgacccag 1560 acgctgacgc tcaacagcgt gcgcgatgtg acggtgaagt ccggcggcag ccgcgacaac 1620 ggcgccacgc tctacgccga cggcagcgac ggcggccagg tgttgcgcgg cctgatggcg 1680 tggaatgtgt cgtccgccgc gggcaagacc ctcaccggcg cgcaggtcaa gctgcaggtc 1740 agcgaccgct ccaccggcac ctacgacctg taccgcgccg gcgctgcctg gaccgaggcc 1800 aacgcgagct acagcggcgt gagcctgggc tccaagatcg gctcggtcgt gcccagcgcc 1860 accggtgcgc aatccatcgc gctcaatgcc gcgggcttca gctggtgaag gactgggcgt 1920 cggcgcggtc gccaacgacg gcgtggtgct gtcttcgacc tccagcgacg gcgtggattg 1980 gtcctcgcgc gaagggccca atcgccggca ggctgatcct gacctacacg ccctgattcg 2040 gcgtttgatt cggcggcttg agcttgtgcg ccgaagacaa acgaaaacgc ccgcgcaatg 2100 ccgggcgttt tcgttgccgc ggacgcgcgg tcaggccatg gcttgcaagg ccgggcgcgc 2160 ggcgtgggtg cccgcgccgg cgccgatcat cagcttgacc gattccttga tgcgctcggc 2220 gccgccgagc ttgaccgcgg cgatcagcga tcgccatggt cttgcttcgt cggctgctgg 2280 cgtcccgaga tg 2292 // |
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