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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X56466; SV 1; linear; genomic DNA; STD; PRO; 1391 BP.
XX
AC   X56466;
XX
DT   05-FEB-1992 (Rel. 30, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 2)
XX
DE   P.fluorescens lepA (partial) and lep gene for leader peptidase 1
XX
KW   cytoplasmic membrane anchored; leader peptidase 1; lep gene; lepA gene;
KW   novel protease class; proteolytic enzyme;
KW   signal sequence cleavage of pre-proteins.
XX
OS   Pseudomonas fluorescens
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
XX
RN   [1]
RP   1-1391
RA   Black M.T.;
RT   ;
RL   Submitted (01-SEP-1991) to the EMBL/GenBank/DDBJ databases.
RL   M.T. Black, Smithkline Beecham Pharmaceutical, Research Division, Brockham
RL   Park, Betchworth, Surrey RH3 7AJ, UK
XX
RN   [2]
RP   1-1391
RX   PUBMED; 1546969.
RA   Black M.T., Munn J.G.R., Allsop A.E.;
RT   ""On the catalytic mechanism of prokaryotic Leader Peptidase 1"";
RL   Biochem. J. 282:539-544(1992).
XX
DR   CABRI; NCIMB 10586.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1391
FT                   /organism="Pseudomonas fluorescens"
FT                   /strain="NCIMB 10586"
FT                   /mol_type="genomic DNA"
FT                   /db_xref="taxon:294"
FT   CDS             <1..486
FT                   /codon_start=1
FT                   /transl_table=11
FT                   /gene="lepA"
FT                   /db_xref="GOA:P26843"
FT                   /db_xref="InterPro:IPR000640"
FT                   /db_xref="UniProtKB/Swiss-Prot:P26843"
FT                   /protein_id="CAA39838.1"
FT                   /translation="GTQVQVTYDMPMNEVVLDFFDRLKSTSRGYASLDYHFDRYQSANL
FT                   VKLDLLINGDKVDALALIVHKDNAHYKGRQLTEKMKELIPRQMFDVAIQAAIGGQIIAR
FT                   TSVKALRKNVLAKCYGGDVSRKRKLLEKQKAGKKRMKQVGNVEVPQEAFLAVLRLDS"
FT   RBS             484..488
FT   CDS             492..1346
FT                   /transl_table=11
FT                   /gene="lep"
FT                   /product="leader peptidase 1"
FT                   /db_xref="GOA:P26844"
FT                   /db_xref="HSSP:1KN9"
FT                   /db_xref="InterPro:IPR011056"
FT                   /db_xref="UniProtKB/Swiss-Prot:P26844"
FT                   /protein_id="CAA39839.1"
FT                   /translation="MSLNFPLLLVIAVAVCGLLALLDLVFFAPRRRSAIASYQGSVSQP
FT                   DAVVIEKLNKEPLLVEYGKSFFPVLFIVLVLRSFLVEPFQIPSGSMKPTLDVGDFILVN
FT                   KFSYGIRLPVIDKKVIEVGDPQRGDVMVFRYPSDPNVNYIKRVVGLPGDVVRYTSDKRL
FT                   FINGESVAEKLLGAEPNTLGSAELYQEKLGAVEHEIRKEMSRYRAMPDGQWKVPAGHYF
FT                   MMGDNRDNSNDSRYWDDPNIPKDLLGMVPDENIVGKAFAVWMSWPEPKLSHLPNFSRVG
FT                   LIK"
FT   terminator      1353..1374
FT                   /note="rho-independent terminator"
XX
SQ   Sequence 1391 BP; 305 A; 388 C; 412 G; 286 T; 0 other;
     ggtacccagg tgcaagtgac ctacgatatg ccgatgaacg aagtggtcct ggacttcttc        60
     gaccgtctca aatccaccag tcgcggctat gcttcgctgg attaccattt cgatcgttac       120
     caatcggcta atctggtgaa actggatctg ctgatcaacg gcgacaaggt agatgccctg       180
     gcactgatcg tgcacaagga caacgcgcac tacaaaggtc gccaactgac cgagaagatg       240
     aaagaactga ttccgcgcca gatgttcgac gttgcgatcc aggccgccat tggcgggcag       300
     atcattgcac ggacttccgt caaggctctc agaaagaacg tactggccaa atgctacggc       360
     ggtgacgtaa gccggaagcg caagctgctt gagaagcaaa aggccggtaa aaaacgcatg       420
     aagcaagtag gtaacgtgga agttccacaa gaagccttcc ttgcggtgct caggttggat       480
     agttaggtcc tatgtcacta aatttcccgc tgttgctggt tatcgccgtc gccgtttgcg       540
     gcctcctggc gttgctcgat ctggtgttct tcgccccgcg tcggcggtcg gccattgctt       600
     cctatcaggg cagcgtcagc cagcccgatg cggtggtgat cgagaagctg aacaaagagc       660
     ccttgctggt tgaatacggc aagtcgttct tcccggtgtt gttcatcgtg ctggtgctgc       720
     gttcgttcct ggtggagccg ttccagatcc cttccgggtc gatgaagccg accctggacg       780
     tgggcgactt tatcctggtg aacaagtttt cctacgggat ccgcttgccg gtgatcgaca       840
     agaaagtcat cgaggtcggt gacccgcagc gcggcgatgt gatggtattc cgctacccga       900
     gcgaccccaa cgtcaactac atcaagcgtg tagtcggcct gccgggcgac gtggtgcgct       960
     acaccagcga caagcgcctg ttcatcaacg gtgaatcggt ggccgagaag ctgctgggcg      1020
     ccgagccgaa caccctgggc agcgccgagt tgtaccagga aaaactcggt gcggtagagc      1080
     acgagatccg caaggaaatg agccgctacc gcgcaatgcc tgatggccag tggaaagtac      1140
     ccgccgggca ctacttcatg atgggcgaca accgcgacaa ctccaatgac agccgttact      1200
     gggatgaccc caatattccc aaggacctgc tgggcatggt gcccgacgag aacatcgtcg      1260
     gcaaagcctt cgcggtctgg atgagttggc cggagcccaa gctcagccac ctgccgaact      1320
     tctcgcgggt cgggctgatc aagtaataca ggcggcgctg tgaacacagc gccgaatgct      1380
     tttctggggt t                                                           1391
//
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