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EBI DbfetchID X55299; SV 1; linear; genomic DNA; STD; PRO; 3360 BP. XX AC X55299; XX DT 27-APR-1993 (Rel. 35, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 6) XX DE C.stercorarium celZ gene for endo-beta-1,4-glucanase (Avicelase I) XX KW avicelase; cellulase; endo-beta-1,4-glucanase. XX OS Clostridium stercorarium OC Bacteria; Firmicutes; Clostridia; Clostridiales; Clostridiaceae; OC Clostridium. XX RN [1] RP 1-3360 RX DOI; 10.1007/BF00265062 RX PUBMED; 2250652. RA Jauris S., Ruecknagel K.P., Schwarz W.H., Kratzsch P., Bronnenmeier K., RA Staudenbauer W.L.; RT "Sequence analysis of the Clostridium stercorarium celZ gene encoding a RT thermoactive cellulase (Avicelase I): identification of catalytic and RT cellulose-binding domains"; RL Mol. Gen. Genet. 223(2):258-267(1990). XX FH Key Location/Qualifiers FH FT source 1..3360 FT /organism="Clostridium stercorarium" FT /strain="NCIB 11745" FT /mol_type="genomic DNA" FT /db_xref="taxon:1510" FT CDS 241..3081 FT /transl_table=11 FT /gene="celZ" FT /product="endo-beta-1,4-glucanase (Avicelase I)" FT /db_xref="GOA:P23659" FT /db_xref="HSSP:1G87" FT /db_xref="InterPro:IPR013783" FT /db_xref="UniProtKB/Swiss-Prot:P23659" FT /protein_id="CAA39010.1" FT /translation="MRKFWSFAIIISLLVTGLFIHTPKAEAAGYNYGEALQKAIMFYEF FT QRSGKLPENKRDNWRGDSGLNDGADVGLDLTGGWYDAGDHVKFNLPMAYSQTMLAWAAY FT EAEEALERSGQMGYLLDAIKWVSDYLIKCHPSPNVFYYQVGDGHLDHSWWGPAEVMQMD FT RPAYKVDLANPGSTVVAEAAAALASAAVVFADRDPAYAATCIQHAKELYNFAEITKSDS FT GYTAASGFYDSHSGFYDELSWAGVWLYLATGDETYLNKAEQYVAYWGTEPQTNIISYKW FT AHCWDDVHYGACLLLAKITGKQIYKEAIERHLDYWSVGYNGERVHYTPKGLAWLDSWGS FT LRYATTTAFLASVYADWEGCSREKAAIYNDFAKQQIDYALGSSGRSYVVGFGVNPPKRP FT HHRTAHSSWADSMSVPDYHRHVLIGALVGGPGKDDSYTDDINNYINNEVACDYNAGFVG FT ALAKMYEDYGGSPIPDLNAFEEITNDEFFVMAGINASGQNFIEIKALLHNQSGWPARVA FT DKLSFRYFVDLTELIEAGYSASDVTITTNYNAGAKVTGLHPWNEAENIYYVNVDFTGTK FT IYPGGQSAYRKEVQFRIAAPQNTNFWNNDNDYSFRDIKGVTSGNTVKTVYIPVYDDGVL FT VFGVENGIKYGNTYLREGTDYTVSGDTVTILKSFLNSFDTSTVQLIFDFSAGRDPVLTV FT NIIDTTTSASIVPTTADFDKNPDASRDVKVKLVPNGNTLLAVKKDGEALVLGRDYSIDG FT DEVTIFREYLADQPVGRVTLTFDFDRGTDPVLTINITDSRQVETGVIQIQMFNGNTSDK FT TNGIMPRYRLTNTGTTPIRLSDVKIRYYYTIDGEKDQNFWCDWSSVGSNNITGTFVKMA FT EPKEGADYYLETGFTDGAGYLQPNQSIEVQNRFSKADWTDYIQTNDYSFSTNTSYGSND FT RITVYISGVLVSGIEP" FT RBS 224..233 FT /standard_name="Shine-Dalgarno" XX SQ Sequence 3360 BP; 1002 A; 643 C; 812 G; 903 T; 0 other; aatgattatt cctttaatcc gacggcaagc agttatgtgg attttaataa aattacggca 60 tacatttctg ggaatctggt atacggtata gagccgtaat agcggattaa aaacaacggg 120 catgatggga tatgacgttc aattgatcat aatttcgggt gcatggttac catgcacccg 180 gaattagacg ttgtatatgg tataatacat ttgaaaccgt tgaagggagg aggttttaat 240 atgcgaaaat tttggtcctt tgcaattata atcagtttac ttgtcaccgg tctctttata 300 catactccta aggctgaagc ggcaggatat aattatgggg aagcattaca aaaagctatt 360 atgttttatg aatttcaaag gtctggaaaa ctgccggaaa acaaaaggga taattggcgg 420 ggagattccg gacttaacga cggagcggac gttggccttg atcttaccgg cggttggtat 480 gatgccggtg atcacgtaaa gtttaacctt ccaatggcct attcccagac aatgcttgca 540 tgggctgcgt atgaggccga agaagctttg gaacgaagcg ggcaaatggg ctatcttttg 600 gatgccatca aatgggtcag cgattacctt ataaaatgcc acccttcgcc aaatgtattt 660 tattatcagg tcggggacgg tcatctggat cactcgtggt gggggccggc tgaagtaatg 720 cagatggaca ggccggcgta taaagtggat ttggctaatc ccggctctac ggtagtcgcc 780 gaagccgctg cggctttggc atcagcagcg gtggtatttg cggacagaga ccctgcatac 840 gcggcgacat gtatacagca tgcgaaggaa ctgtataact ttgctgaaat taccaagagt 900 gacagcggat ataccgcagc cagtggtttt tatgattcac acagcggctt ctatgacgag 960 ctttcatggg ctggtgtatg gctatacctg gcgaccggag atgagacata tcttaacaag 1020 gccgaacagt atgtagccta ttggggaacg gagccgcaga ccaatataat atcatataaa 1080 tgggcacatt gctgggacga tgttcattac ggcgcatgcc tgcttttggc taaaataacc 1140 ggtaagcaga tttataagga agctattgag agacatctgg attattggtc ggtaggatat 1200 aacggtgaac gggtacatta tacgcctaaa gggctggcat ggcttgacag ctggggttct 1260 ctccgttatg cgaccaccac cgcatttttg gccagtgttt acgccgactg ggaaggctgc 1320 agcagggaga aagccgccat atacaatgat tttgcaaaac agcagattga ttatgcttta 1380 ggaagttccg gcagaagcta tgtggtaggt ttcggagtaa atccccccaa aaggcctcat 1440 cacagaacag cccacagttc ctgggccgac agcatgagcg tccccgatta tcacagacat 1500 gtacttatcg gagctttggt gggaggaccc ggaaaagatg acagttatac cgatgatata 1560 aacaattata taaataatga agtagcctgc gactataatg ccggatttgt cggtgctctc 1620 gccaagatgt atgaagatta tggaggaagt ccgatacccg atttgaacgc ctttgaagaa 1680 ataacaaatg atgaattttt tgtcatggcg ggtatcaatg cttccgggca aaacttcata 1740 gaaattaagg ctctgcttca taaccagtcc ggttggcccg caagagtagc cgacaaactt 1800 tcattccgtt actttgtgga tttaaccgag cttattgaag caggatacag tgcaagtgat 1860 gttacaataa ccaccaacta caacgccgga gctaaagtta cgggcctgca tccgtggaat 1920 gaggccgaaa atatctatta tgtaaatgta gactttaccg gaaccaaaat ttatccgggc 1980 ggtcagtcgg catacagaaa ggaagtccag ttcagaattg cggctcctca gaacaccaat 2040 ttctggaata atgataatga ctattctttc agggacatta aaggagtaac atcgggtaat 2100 accgttaaga cggtttatat acctgtatat gatgacgggg ttctggtatt tggagtagag 2160 aacggaatta agtacggaaa tacttattta agggaaggga cggattatac ggtatccggc 2220 gatacggtta caattctgaa aagttttctt aattccttcg ataccagtac tgttcagctt 2280 atatttgatt tcagcgcagg aagagatccg gttcttacag taaacattat tgataccact 2340 accagtgctt caattgtgcc gactacggca gactttgaca aaaatcctga cgcaagccgg 2400 gatgtgaagg ttaaattagt gccgaacgga aatactcttt tagcggttaa aaaggacggc 2460 gaggctttgg ttttgggtag ggattacagt attgacggtg atgaggttac tattttcagg 2520 gaatatcttg cagaccagcc tgtgggcagg gttactctga cctttgattt tgacagagga 2580 accgatcctg ttctgacaat taatataacc gattcaaggc aggtagaaac gggagttatt 2640 cagatacaga tgttcaacgg caatacttcc gataaaacaa acggaataat gccgagatac 2700 aggcttacca ataccggaac aactccgatt agattatccg atgttaaaat ccgttactac 2760 tataccattg acggggaaaa ggatcagaac ttctggtgtg actggtccag tgtaggaagc 2820 aataatataa ccgggacttt tgtcaaaatg gctgaaccca aagaaggcgc ggattattac 2880 ctggaaaccg gctttaccga tggagcgggt tatctgcagc ccaatcagag tattgaggtt 2940 caaaacaggt tctccaaagc tgactggaca gattacattc aaaccaatga ttattccttc 3000 agcacaaata cctcttacgg cagtaatgac aggatcactg tatacatttc aggcgtgttg 3060 gtcagtggaa ttgaaccgta atttgcaaat taaagagttt acttattcca agaaataaag 3120 tttgctgccg ctttaacctc tattaagtgg aagcggcagc tttttctatt ttattggaag 3180 taatttttcc gtaaatcaat tttcagtttg attacgcaaa ctatctaatt cttatataag 3240 tgatgtataa gtcttttcta atagatagta accggtagta tgtgccgaat aacgaacccc 3300 aaaatataca aaattctcaa aaaacttaaa agaaaatata tacgatttaa tgaaaggaca 3360 // |
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