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EBI DbfetchID X55140; SV 1; linear; genomic DNA; STD; PRO; 3681 BP. XX AC X55140; XX DT 24-APR-1991 (Rel. 28, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 6) XX DE H.giganteus hgiDIR & hgiDIM genes and ORF(metB) for restriction enzyme DE HgiDIR and methyltransferase HgiDIM. XX KW hgiDIM gene; hgiDIR gene; methyltransferase; restriction enzyme. XX OS Herpetosiphon aurantiacus OC Bacteria; Chloroflexi; Herpetosiphonales; Herpetosiphonaceae; OC Herpetosiphon. XX RN [1] RP 1-3681 RA Kroeger M.; RT ; RL Submitted (27-NOV-1990) to the EMBL/GenBank/DDBJ databases. RL Kroeger M., Inst. Mikro- and Molekularbiologie, JLU Giessen, Frankfurter RL Strasse 107, D-W-6300 Giessen, Germany. XX RN [3] RP 1-3681 RX DOI; 10.1093/nar/19.5.1049 RX PUBMED; 2020544. RA Duesterhoeft A., Erdmann D., Kroeger M.; RT "Stepwise cloning and molecular characterization of the HgiDI RT restriction-modification system from Herpetosiphon giganteus Hpa2"; RL Nucleic Acids Res. 19(5):1049-1056(1991). XX CC See also X55137-X55143. XX FH Key Location/Qualifiers FH FT source 1..3681 FT /organism="Herpetosiphon aurantiacus" FT /strain="Hpa2" FT /mol_type="genomic DNA" FT /db_xref="taxon:65" FT CDS 676..1755 FT /transl_table=11 FT /gene="hgiDIR" FT /product="restriction enzyme" FT /db_xref="GOA:P24599" FT /db_xref="InterPro:IPR019044" FT /db_xref="UniProtKB/Swiss-Prot:P24599" FT /protein_id="CAA38937.1" FT /translation="MFEQLNQPGLFGITNSNRDFTKKEAWGKNQFNNAFPIALACFMFS FT QNIKPIYIRLEKRNIEHNYFAVDQVFQINPLEAQAFFAFEHSYHPYTELIIGKTPAIDV FT VISNLQNSQIINAFEIKLTAIPDNTTANLPDNLQGCEIVIRPDTIVYLALSIAKVFQQN FT PLALLDILDPVCARIGDWEDATSIQPMIPLFCELLYTIFDRYQAVQIPILLQPIWKTQG FT KLSILHENCLDLFVWSNFALAKVFLDASIKPSEKSITRPERTTVWLIKMLYDFAQNGKI FT DYKRTLDRITFNLKNDKAFAASGMVTRKYMNSPELQNPRIKRHSIKHIIINGGQRYLSP FT ERRLDSAIVSTPGLFEEIL" FT CDS 1752..2681 FT /transl_table=11 FT /gene="hgiDIM" FT /product="methyltransferase" FT /db_xref="GOA:P24600" FT /db_xref="HSSP:1DCT" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P24600" FT /protein_id="CAA38938.1" FT /translation="MKTIDLFAGCGGMSLGFMQAGFEIVAAVDNWRPAINTYQQNFTHP FT IHELDLAQIDAAVSLIKTHSPELIIGGPPCQDFSSAGKRDEGLGRANLTLDFAKIVLAI FT QPAWVIMENVERARLSKIHQQACSMLGDEGYSLAQVVLDASLCGVPQLRKRTFVIGHRH FT GSIADLANVLQQRLAKQSLTVRDYFGESLDTDYYYRHPRTYERRAIFSVNEPSPTIRGV FT NRPIPATYRMHPKDAGDVSLARPLTTKERSLIQTFPLDFKFVGTKSEQEQMIGNAVPVN FT LAFFLATSLQAYLNQPRMQQLSLLPSFF" FT CDS 2725..>3681 FT /transl_table=11 FT /note="ORF homologous to E.coli metB" FT /db_xref="GOA:P24601" FT /db_xref="HSSP:1GC0" FT /db_xref="InterPro:IPR015422" FT /db_xref="UniProtKB/Swiss-Prot:P24601" FT /protein_id="CAA38939.1" FT /translation="MDYGFATRAIHAGQDPESVTGAVIVPIYQTSTYAQRGVGDHTGYE FT YSRTDNPTRTALQTCLAALEEAKHALVFASGLGASTTLMLMLKAGDHVICGDDVYGGTY FT RLFQRVMTEHGLSFDFIDMADPEAVRAAIKPNTRLIWLETPTNPLLKLAPIAAITRVAR FT EHGIWTIVDNTFASPYNQRPITLGADMVLHSTTKYIGGHSDVVGGAIMTSNDELYEKLK FT FLQNAAGAVPGPFDCWLVLRGVKTLSIRMRDDERNALAIAQFLTEHPGVEKVIYPGLPS FT HPQHNLAREQMRGFGGMISILLKGGAEAANAMVSKTKL" XX SQ Sequence 3681 BP; 1012 A; 765 C; 801 G; 1103 T; 0 other; agatctaccc caagttttat ttccgttaga tcaagtctgc atgggcttgg ctgcatggtt 60 gggtgataca gctatggcgt agccattcga gcagcagctg tatcaactgg cgcaacaagg 120 gcttgagcat ttgccgcgtg gctgtgccat aacgatattc gcgctgccaa tctgttgttc 180 aaaggtgatc agctgcaacg gttttagatt ttgaagaagt tggctggcgc tgcttggtgc 240 tggaattggc gtggacagca gttcatggct tgacgatgta tcacaattgg caaccagcta 300 cggcgctcaa caacaagcaa ttatcgctgg ctatcaaacg cttcggcctt tgaatcctga 360 tgagctggcg attttgccaa acttgtgtcg tttacaaagt ctttaccttt tatgttcgca 420 aggttctacg gcgctggatg caattgagcg cttaaaagaa ttaaattggg attaaaaatc 480 acccctaaat caccagcatg tgggatggct tttggcctaa attgcgctat actgaaatca 540 aaccttctcc tctctcttct ctacaaggct ccattatgcg agccgtttgt ggttttaggc 600 gcttatattt ctatgtatgc tataattaaa ctcgataaat tattctaata aatggcatat 660 ttaggaagac ctagaatgtt tgagcaatta aatcaaccag gattgtttgg aataactaat 720 tctaaccgag attttacaaa aaaagaagct tgggggaaaa accaatttaa taatgcattt 780 cctattgctt tagcttgttt tatgtttagc cagaacatta aacctatcta tattcgatta 840 gaaaagcgga atattgaaca taactatttt gctgttgatc aagtatttca aattaatcct 900 ttagaagcac aggcattttt tgcatttgaa catagctatc atccatatac tgaacttatt 960 attgggaaaa caccagcaat tgatgttgtc attagcaatc tgcaaaactc tcaaatcatt 1020 aatgcttttg agataaaatt aaccgcaatt cctgataata caacagcaaa cctcccagat 1080 aatcttcaag gttgtgaaat tgttattcga cctgatacca ttgtctatct agcactcagt 1140 attgctaaag tatttcaaca aaatccgtta gcgcttttag atattttaga tcctgtctgt 1200 gctagaattg gtgattggga agatgcaact agcattcaac caatgattcc actattttgt 1260 gaattactgt atacaatttt tgatcgttat caagctgttc aaataccgat tttacttcaa 1320 ccaatttgga aaactcaagg aaaattatca atactacacg aaaattgcct tgatcttttt 1380 gtatggagca attttgccct ggcaaaagtt tttttagatg cttcgataaa accttctgag 1440 aaatcaatta ctcggcctga acgtacaacc gtttggttaa tcaaaatgct ctatgatttt 1500 gctcagaatg gcaaaattga ttataaacga acgcttgatc ggattacctt taatttgaaa 1560 aatgataaag cctttgctgc tagcggaatg gtcacaagaa aatatatgaa ttcgccagaa 1620 ttacagaatc cacgaataaa acgccattca attaaacata ttattattaa tggtggtcag 1680 cgttatctta gccctgaacg gcgtttggat agtgcaattg tgagtacacc tggccttttt 1740 gaggaaatac tatgaaaact attgatttat ttgctggctg tggtggcatg tctttaggat 1800 ttatgcaagc aggatttgag atagttgcgg cagttgataa ttggcgacct gcaattaata 1860 cctatcagca aaattttact cacccaattc atgagcttga tttggcacag attgatgcgg 1920 ctgtatcctt gatcaaaacg catagcccag aattaattat tggtggacct ccgtgtcagg 1980 atttttcctc agcaggtaaa cgtgatgaag gtttggggcg tgccaactta acccttgatt 2040 ttgccaaaat agtactagct attcaacctg catgggttat catggaaaat gttgagcgtg 2100 ctcgactttc caaaattcat caacaagcgt gtagtatgtt gggggatgaa ggctattctt 2160 tggctcaagt tgtattagat gctagtttgt gtggcgtgcc tcaattacgc aaacggacgt 2220 ttgtgattgg tcatcgtcat ggttcaatcg ctgatttagc aaatgtgctt cagcaacgtt 2280 tagccaaaca atcattaacg gttcgcgatt attttggtga aagtttagat actgactatt 2340 attatcgcca tccgcgaacc tatgagcggc gcgctatctt tagtgttaat gaaccaagcc 2400 caacaattcg tggggtgaac cgaccaattc ctgcaaccta tcgcatgcat cctaaagatg 2460 ctggcgatgt gtcgttagca cgaccgctga ctaccaaaga acggagtctt atccagacat 2520 ttccattaga ttttaagttt gttggaacga aatctgagca agagcagatg attggtaatg 2580 cagtgccagt taatttggcg ttttttctag caaccagtct tcaggcttat cttaatcagc 2640 ctaggatgca acaactatca ttattaccat ctttctttta atgtaatgat caatgttgat 2700 tattaacgaa taaggagcac actcgtggat tatggctttg caacccgcgc catccatgcc 2760 gggcaagacc cagaatcagt aactggcgca gtcatcgtgc caatttatca gacttcaacc 2820 tatgcccaac gtggcgttgg cgaccataca ggctatgaat attcgcgcac cgataaccca 2880 actcgcaccg cgctccaaac atgtttagcc gctttggaag aggcaaaaca cgccttggtt 2940 tttgcttcgg gcttgggcgc ttcgacaacc ttgatgctca tgctcaaagc tggcgatcat 3000 gtaatttgtg gcgatgatgt ttatggcggc acttatcgtt tgtttcaacg ggtgatgacc 3060 gagcacggcc tgagctttga tttcattgat atggctgatc ctgaggcagt gcgggccgcg 3120 attaagccca atacgcgctt gatttggttg gaaactccca ccaatccgtt gttgaaactg 3180 gctccgattg ccgcgattac gcgggttgcg cgtgagcatg gcatttggac gattgtcgat 3240 aatacctttg cctcgcctta caaccaacgc ccaatcacct tgggagctga tatggtctta 3300 cacagcacga ccaaatatat tggcggccat agcgatgtgg ttggcggcgc aattatgacc 3360 tcgaacgacg agctgtatga gaaattgaag tttttgcaaa atgctgccgg tgctgtgcca 3420 ggcccctttg attgctggtt ggtgctgcgt ggggtcaaaa ccttgagcat tcgcatgcgt 3480 gacgacgaac gcaacgcttt ggcaattgcc caatttttaa ccgaacatcc tggggttgaa 3540 aaagtgattt acccaggctt gccatcgcac ccacaacaca atttggcccg cgagcaaatg 3600 cgcggttttg gcggcatgat ctcgattttg ctcaaaggtg gcgctgaggc agccaatgcc 3660 atggttagca aaactaagct t 3681 // |
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