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EBI DbfetchID X53868; SV 1; linear; genomic DNA; STD; PRO; 2134 BP. XX AC X53868; XX DT 27-APR-1993 (Rel. 35, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 3) XX DE Moraxella sp. lip2 gene for lipase 2 XX KW LIP2 gene; lipase 2; secreted protein. XX OS Moraxella sp. OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; OC Moraxellaceae; Moraxella. XX RN [1] RP 1-2134 RA Feller G.; RT ; RL Submitted (06-JUL-1990) to the EMBL/GenBank/DDBJ databases. RL Feller G., Laboratory of Biochemistry, University of Liege, Institute of RL Chemistry B6, Sart Tilman, B-4000 Liege, Belgium. XX RN [2] RX DOI; 10.1089/dna.1991.10.381 RX PUBMED; 1907455. RA Feller G., Thiry M., Gerday C.; RT "Nucleotide sequence of the lipase gene lip2 from the antarctic RT psychrotroph Moraxella TA144 and site-specific mutagenesis of the conserved RT serine and histidine residues"; RL DNA Cell Biol. 10(5):381-388(1991). XX DR CABRI; LMBP 3230. XX CC excreted enzyme; antarctic strain XX FH Key Location/Qualifiers FH FT source 1..2134 FT /organism="Moraxella sp." FT /strain="TA144" FT /mol_type="genomic DNA" FT /clone_lib="genomic expression" FT /clone="pULG332" FT /cell_line="E.coli RR1" FT /db_xref="taxon:479" FT -35_signal 540..545 FT -10_signal 569..574 FT RBS 584..588 FT CDS 594..1895 FT /transl_table=11 FT /gene="lip2" FT /product="triacylglycerol lipase" FT /EC_number="3.1.1.3" FT /db_xref="GOA:P24484" FT /db_xref="InterPro:IPR002168" FT /db_xref="InterPro:IPR013094" FT /db_xref="UniProtKB/Swiss-Prot:P24484" FT /protein_id="CAA37862.1" FT /translation="MPILPVPALNALLTKTIKTIKTGAAKNAHQHHVLHHTLKGLDNLP FT APVLERINRRLKASTAEQYPLADAHLRLILAISNKLKRPLAIDKLPKLRQKFGTDAVSL FT QAPSVWQQNADASGSTENAVSWQDKTIANADGGDMTVRCYQKSTQNSERKSTDEAAMLF FT FHGGGFCIGDIDTHHEFCHTVCAQTGWAVVSVDYRMAPEYPAPTALKDCLAAYAWLAEH FT SQSLGASPSRIVLSGDSAGGCLAALVAQQVIKPIDALWQDNNQAPAADKKVNDTFKNSL FT ADLPRPLAQLPLYPVTDYEAEYPSWELYGEGLLLDHNDAEVFNSAYTQHSGLPQSHPLI FT SVMHGDNTQLCPSYIVVAELDILRDEGLAYAELLQKEGVQVQTYTVLGAPHGFINLMSV FT HQGLGNQTTYIINEFACLVQNLLTSEGDKPNLRA" FT stem_loop 1895..1913 FT stem_loop 1970..1995 XX SQ Sequence 2134 BP; 591 A; 459 C; 479 G; 605 T; 0 other; atcgatgtca ttttcgatgg tctcattagc gtcagtatct gaattatttt tcggatcaaa 60 ctctttcatg gtttcttcta aaatactctc agcgtgctca acattgtcat gtgacacgtt 120 ttggtcatga gattcatggt tggtgttctg ctcgctcatg gtagctcctt aggattatta 180 ataataatgg ttggttttaa ggcacaataa actcgccaaa cgctggttat tgtgctttta 240 ttaatggcct aactgttttt gttcgttctt tttaaaatgc gcttcaatag ctgcttgata 300 aaggtggtga ggataaattt caagcccaac ctgaggaatt ttttgtttta gcaggataaa 360 tcattgcaaa gcgtaaggat tattactaat accgctataa aaagtgctca acattaggat 420 aactggctgg tagagacgat tacgtttttg tcaccgatat gcgcttaact ttatcgtgac 480 tagcttttaa gatagattgg aatttaatat aaatttcacg attaacgcta tattgacagt 540 tgactgataa agccgactga ctttgtttta acaccaatca caagagaggg tttatgccta 600 ttctaccagt accggcgctc aatgccttgc tgacaaaaac gattaaaacc attaaaactg 660 gggccgctaa aaatgctcat cagcatcatg ttttgcacca tacgctaaaa ggattggaca 720 atctgccagc gccggtgctc gaacgtatca atcgtcgatt aaaggcgtct acagcagagc 780 aatatcctct agccgatgcc catctgcgcc tgatactcgc tattagtaat aagctcaagc 840 gaccgttagc aatagataag ctgcccaaac tacggcagaa atttggtact gatgcggtgt 900 ctttgcaagc gcctagcgtt tggcaacaaa atgcggatgc ttcaggcagt acagaaaatg 960 ccgtcagctg gcaagataaa accattgcta atgccgatgg tggtgatatg accgtgcgct 1020 gctaccagaa atcgacgcaa aatagtgaga gaaaaagtac ggatgaagcc gccatgctat 1080 tttttcatgg cggcggattt tgtatcggtg acatcgacac gcaccatgag ttttgtcata 1140 cggtctgtgc tcaaacgggc tgggcggtgg taagtgtcga ctatcgcatg gcacctgaat 1200 atccagcgcc aactgcactt aaggactgtc tggcggctta tgcttggctg gctgaacatt 1260 cacagtcgct tggcgcatca ccatcgcgta ttgtactatc tggtgatagt gcgggcggtt 1320 gcttggcagc actggttgca cagcaggtta tcaaacctat cgatgcacta tggcaagata 1380 ataatcaagc tcctgcagcc gataaaaaag tcaatgacac ttttaaaaac tcactggctg 1440 atttaccgcg acctttagca caactgccgc tatatcctgt cactgattat gaggctgaat 1500 atccaagctg ggagctttat ggtgaagggt tgctgctcga tcacaacgat gcagaagtct 1560 ttaattctgc ctacactcag cacagcggtc taccacagtc ccatccactt atctcagtca 1620 tgcatggtga caatacgcaa ctgtgtccca gctatattgt cgttgcggaa ttagatatct 1680 tacgagatga aggactcgcc tatgccgaac ttttgcaaaa agagggcgtt caagtacaaa 1740 cttatacagt gctaggagcg ccgcacggtt ttattaactt aatgagtgtc catcaaggtc 1800 ttggcaatca gacaacttat attatcaatg agtttgcttg tcttgtgcaa aacctactga 1860 ccagtgaggg tgataagcca aatctcagag cgtgatccca aacgaaatgg gataccccaa 1920 aagccgtaac cagatgacac tacgaagtga ctgttattga tgacctcata gccataacct 1980 aagcggttga tggctttgat gataaaattg gcaggaatat ttgtccatta tgggtatgcc 2040 ctgagagctg taaatcaata ggtaactcac tgtgctcggt aatagcgctc ggtctgtgat 2100 catgagtcgt ctgaactcta ccgttgccga tatc 2134 // |
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