 |
|
EBI DbfetchID X53373; SV 1; linear; genomic DNA; STD; ENV; 5741 BP. XX AC X53373; XX DT 22-JUL-1991 (Rel. 28, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 6) XX DE Unclassified alcalophilic gram-positive bacteria DSM 5853 amyA180 gene for DE exo-maltopentaohydrolase (amylase) XX KW amyA gene; amylase; exo-maltopentaohydrolase. XX OS unidentified bacterium OC Bacteria; environmental samples. XX RN [1] RP 1-5741 RA Candussio A.; RT ; RL Submitted (30-MAY-1990) to the EMBL/GenBank/DDBJ databases. RL Candussio A., Lehrstuhl fuer Mikrobiologie der Universitaet Muenchen, RL Maria-Ward-Str. 1a, D-8000 Muenchen 19, FRG. XX RN [2] RP 1-5741 RX DOI; 10.1111/j.1432-1033.1990.tb19108.x RX PUBMED; 1696201. RA Candussio A., Schmid G., Boeck A.; RT "Biochemical and genetic analysis of a maltopentaose-producing amylase from RT an alkaliphilic gram-positive bacterium"; RL Eur. J. Biochem. 191(1):177-185(1990). XX FH Key Location/Qualifiers FH FT source 1..5741 FT /organism="unidentified bacterium" FT /environmental_sample FT /strain="UGO163/26" FT /mol_type="genomic DNA" FT /db_xref="taxon:2338" FT promoter 172..177 FT /note="-35 region" FT promoter 196..201 FT /note="-10 region" FT precursor_RNA 212..>212 FT /note="transcript" FT CDS 297..5351 FT /transl_table=11 FT /product="precursor polypeptide (AA -37 to 1647)" FT /db_xref="GOA:Q03658" FT /db_xref="HSSP:1K85" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/TrEMBL:Q03658" FT /protein_id="CAA37453.1" FT /translation="MKQQLNRVISIVLCLIVMLSVFESTIMLLPGSVEVKGQEYRELNQ FT LENKPFSWDNANVYFVLTDRFYNGNTSNDNSYGRPQIDAWGTNIGTFHGGDIKGLTKKL FT EEGYFTDLGTNAIWISAPWEQMHGWVGGKDGDFAHYGYHGYYGLDFTAMDQNMGTIDEM FT REFVDLAHSLGIRVVLDIVMNHVGYPTIVDMHEFGFGDTGGLPRDWTPNQAQGQNWHTH FT NDIMNKDNEAAWANWWGSDWIRADETAGYDNCGGSEQTMCIGFLPDIKTEVTTGVDLPP FT ILRNKWNDQASGYEDWFVPAAEPYRQDLNIAPKDYLIKWITSWVEEFGIDGFRVDTAKH FT VEIERWAELKNEAEVALQTWRENNPDKPGANWDDNFWMTAEVFGHGLGKSEYFDFGFDS FT VINFEFQNANFNNLEGLFSRYANSINTDPDFNMLSYVSSHDTKLYSRDDLIQAGTALLL FT LPGGVQVFYGDETARPLGDGGSDPEQGTRSSMNWANINQNVLSHWQKLGQFRNNHIAIG FT AGAHQKLSDSPYTFARTYESDDIVDEVVVATGAQGTTAVTVEGVFEDGTVVRDAYTGDE FT TTVTKGTATFTAGTQGIILIENTAEPVTNLPIVSATPGNSSFRTDDITITLNVDRADMG FT KYTLDGSDPADGLTFMDGEEIVIGADMEFDETATLRLYAENENGIRTRSYTYRKVDPDA FT LLEVYFKKPADWGTPHIYYYDTFPEEPEVTWTTAPEMTLVEDDWYVYVFENAESANIIF FT KDSSGKQIPGPNEPGFFIDQIGWYDGVKWLDSDPFEREPKEPATTPKNLSVVNVTETTV FT TFEWDQSDGYVVEYEILRDEDVVASTIRTTFTDEDLNPDTTYTYSVVAVGEGGQKSAPS FT EALKVTTLEENDEPKEPAEAPENLRIADITDTTVTINWNASNGYVTGYEVLRDGVVIGE FT TTRTTFIDTGLDADRTYTYTIVALGDGGQKSDPSEALEVTTQEKPEGNLVTIYYKKGFD FT TPYMHYRPEGGEWTIVPGIRMEESEIAGYSKLTVDIREASKLEVAFNNGRGAWDSDQEN FT NYLFEPGVHTYIPSHEGRGEIIPGKPGAPIDGNKVTIYYQNGFDTPYVHYRPEGGNWTN FT APGLKMEDSEFASYSRLTLDIGEANRAEVAFNNGRGLWDSDNENNYFFNIGDNTYIPGK FT NGSAGEIYGGKPRPPLVGNEVIIYYKNGFDTPYVHYRPEGGTWTNAPGIKMDKSEIAGY FT SKITLDIGRADRVEVAFNDGRGAWDSDNERNYLFVVGNNTYEPGINGAPGQVKHGVLPD FT DGEDPGDIEDPDHTSPSKPTDLTAIATAHTVSLSWTASADDVEVAGYKIYRDGVEIGVT FT ESTTYTDSGLTAETTYSYMVQAYDTSNNFSALSDELTIETAEKTGVDPGGDMPYSTNPS FT FGKKVTTPITIDGVNDGEWTDDMLIAIGMAGDDPRSLGDNWSMHETPMDLTHLWGAWDH FT EYLYLAWQYVDVTDIIDPANAGSSAGTTISQMDMPQTIAIDTIPEQGATHDMWGKNGGE FT SLWGGPDLPDYQLNIASNMFHSGYISRAVDGVFPVDDGGINYKTGEEAGITVKFSKGKG FT YSTLWGVLDADDAVDPSKLVNFTELAHDSTRDTFYEAKIPLAAIGNPDIENERIGVMIH FT QGEFSPMDTLPNDPATSDTPGVSESNSPLEWEDIDLLTVPFARIGQ" FT sig_peptide 297..407 FT /note="signal peptide (AA -37 to -1)" FT mat_peptide 408..5348 FT /note="exo-maltopentaohydrolase (AA 1 to 1647)" FT terminator 5360..5385 FT /note="put. hairpin loop" XX SQ Sequence 5741 BP; 1841 A; 942 C; 1355 G; 1603 T; 0 other; ggtaccgcct atctcagtgt gtgaaagcta tgcatcaaaa tacctactcc atgagcgttt 60 cttcaacacg aatctacttt atttaatatt attcataacg aaacatcaga aaatattgtt 120 attacctaaa ttccttgttt ttgtctttta atgttggtca atgttctatg gttgtgctaa 180 taaaaatgtt aacgctttct caggaggcta tatgagaggg gtgatgtctg ctaaacaata 240 aggattcatc aacaccatgg ttataaaaaa ttaaagattg aaaggaggaa aaggtaatga 300 agcaacagct taatcgcgtg ataagtatcg tattatgttt aattgtcatg ctctcggtgt 360 ttgaaagtac tattatgtta ttaccaggtt cagtagaggt aaaaggccaa gagtatcgag 420 aactaaatca gctagaaaat aaaccttttt catgggataa tgcaaacgtt tactttgtgt 480 taaccgatcg tttttacaat ggaaatacaa gtaatgataa ttcttatggg agaccgcaaa 540 tagatgcttg gggtacaaac attggtactt tccatggcgg ggacataaaa ggattaacaa 600 agaaattgga agaaggttac tttacagacc taggtacaaa tgccatatgg atatctgctc 660 catgggaaca aatgcatggc tgggttggtg ggaaagatgg tgattttgct cactatggct 720 atcatggtta ctatggatta gattttacgg ctatggatca gaatatgggt acaattgatg 780 aaatgcgtga atttgttgac cttgcacatt cattaggcat tagagttgtt ctcgacattg 840 ttatgaatca cgttggctat ccaacgatcg ttgacatgca tgaatttggt tttggtgata 900 ctggaggact tccaagagat tggacaccta atcaagcaca gggccaaaat tggcatacac 960 ataatgacat tatgaataaa gacaatgaag cagcttgggc gaattggtgg ggaagtgact 1020 ggattcgtgc cgatgaaaca gccgggtatg acaattgtgg tggcagcgaa cagacaatgt 1080 gtatagggtt cttaccagat attaaaacgg aagtaaccac aggtgttgat ttaccaccga 1140 tattgagaaa caagtggaat gatcaagcta gtggctatga agattggttt gttccagcag 1200 ctgaacctta tcgtcaagat ttaaacattg ctccgaaaga ttatttgatc aaatggatta 1260 cttcatgggt tgaggaattc ggtattgatg gattccgtgt tgatacagca aagcatgtag 1320 agattgagcg atgggctgaa ttgaagaatg aagcggaagt agcacttcaa acatggcgag 1380 aaaataaccc agataagccc ggtgctaatt gggatgataa tttctggatg acagcagaag 1440 tatttggaca tggtcttggg aaaagcgagt attttgattt tggtttcgat tctgtgatta 1500 attttgaatt ccagaatgca aacttcaata atttagaagg tttattttct agatatgcaa 1560 attcaattaa cactgaccct gatttcaaca tgttaagtta tgtttcatct catgatacaa 1620 agctttatag tagagatgac cttattcagg caggtacagc attactatta cttccaggtg 1680 gcgttcaagt gttttatggc gatgaaacag ctcgaccatt aggggatggt ggttctgatc 1740 ctgagcaagg tacgcgttca tcgatgaatt gggctaatat taatcagaat gtactctctc 1800 attggcaaaa acttggtcaa ttcagaaata atcacatagc tattggtgcg ggagcgcatc 1860 agaagttatc tgatagtccg tatacgtttg cgcgtacgta tgaatcagac gatatagttg 1920 atgaagtcgt cgttgcaact ggggcccaag gaacaacagc tgttactgta gaaggtgttt 1980 ttgaagatgg gacagttgtt cgagatgctt atactggtga tgagacaaca gtaactaaag 2040 ggacagcaac atttactgct ggaacacaag gtattattct aatcgaaaat acagctgagc 2100 cagttactaa tttgccgatc gtttcagcaa cacctggtaa tagttctttt aggacagatg 2160 acataacaat cacgctaaat gttgatcgag cggatatggg gaagtataca cttgatggaa 2220 gtgatccagc agatggccta acgtttatgg atggagaaga aattgtcatt ggtgctgata 2280 tggagtttga tgaaacagca acattgagac tctatgcaga aaatgaaaat ggcataagaa 2340 caaggagtta cacatatagg aaggtagatc cagatgcgtt acttgaagta tattttaaga 2400 aaccagcgga ttggggaaca ccacatatat attactatga tacatttcca gaggagccgg 2460 aagtcacttg gactacagct ccagagatga cattagtaga ggatgattgg tatgtatatg 2520 tttttgaaaa tgctgaaagt gccaatataa tatttaagga ttcttcagga aaacaaattc 2580 caggtccaaa tgaaccagga ttcttcattg atcagattgg ttggtacgat ggcgtaaagt 2640 ggcttgattc agatcctttt gaaagggaac ctaaagagcc tgcgacaaca cctaagaacc 2700 taagtgttgt taatgtaact gaaactactg taacatttga gtgggaccaa tctgatggtt 2760 atgtcgttga atacgagatt ttacgtgatg aggatgttgt tgcttcaact attcgtacaa 2820 catttacgga tgaagacctt aatccagata caacctacac ttattctgtc gtagctgttg 2880 gagaaggcgg gcagaaatcc gccccaagtg aagcgttaaa agtgactaca ttagaagaaa 2940 atgatgaacc taaggaaccg gctgaggcgc cagaaaattt acgtatagct gatataacag 3000 atacaacagt tacaatcaac tggaatgcat ctaatggtta cgtaacagga tatgaggttc 3060 tgcgtgatgg tgtggttatt ggcgaaacaa cacggacaac attcatagat actggattag 3120 atgctgatag gacctatacg tatacgattg ttgctctcgg agatggcggg caaaagtctg 3180 atccgagcga agcgttagaa gtaacaactc aagaaaaacc agaaggaaat ctagtaacaa 3240 tatactataa aaaaggcttt gataccccat atatgcatta tcgtccggaa ggtggagagt 3300 ggacgatcgt tccaggaatt agaatggaag aatcagaaat agcaggctat agtaagttaa 3360 ccgttgatat tcgggaagca agcaagttgg aagtagcctt taataatgga cgtggggctt 3420 gggatagtga tcaagagaat aattatttat ttgagccagg tgttcatacg tacattccga 3480 gtcatgaagg aagaggagag attattccag gtaaaccagg agcaccaatc gatggtaata 3540 aagtgacgat ttactatcaa aatggctttg atacgccgta tgttcattac cgcccagaag 3600 gcggaaattg gaccaacgcc ccaggattaa aaatggaaga ttcagagttt gcaagttata 3660 gtaggttaac gcttgatatt ggtgaagcta atcgtgcaga agtggctttc aataacggac 3720 gcggcctttg ggatagtgat aatgaaaata attatttctt caatattggc gataacactt 3780 atataccagg aaaaaacggt tcagctggag agatttatgg aggtaagcca agaccaccat 3840 tagtaggaaa tgaagtaatc atttattata aaaatggttt tgatacaccg tatgttcatt 3900 atcgtccaga aggtggtacg tggacaaatg caccaggaat aaaaatggat aagtcagaaa 3960 tagcaggtta cagtaaaata acgcttgata ttggtcgcgc agatcgagta gaagtagcct 4020 ttaatgacgg tcgtggtgca tgggatagtg ataacgaacg taattatctc tttgtagtcg 4080 gtaacaatac ttatgaacca ggaattaacg gcgcacctgg tcaggtgaaa catggcgtgt 4140 tacctgatga tggagaagat ccgggagata ttgaagaccc tgatcatacc tccccttcaa 4200 agccgactga tttaacagca atagctactg ctcatactgt ttcattaagc tggacagctt 4260 cagcagacga tgtagaagta gctgggtaca aaatttatcg agatggtgtg gaaatcggtg 4320 ttactgaatc aacaacttat acggattcag gcttaacggc agaaacaacg tatagctata 4380 tggtacaagc ttatgatact tctaataatt tctcggcatt aagtgatgaa ctgacaattg 4440 aaaccgccga gaaaacgggt gttgatccag gaggggatat gccttattcc acgaatccat 4500 cgtttggtaa gaaggtaaca acgccaatca caattgatgg tgttaatgac ggggaatgga 4560 cagatgatat gttgattgca attggtatgg ctggtgacga cccacgttcg ctcggggaca 4620 attggtctat gcatgaaaca ccaatggacc ttactcacct atggggagca tgggaccatg 4680 agtacttgta tcttgcttgg caatatgtag atgtaacaga tattattgac ccagctaacg 4740 caggctcatc agctggtacc acaattagcc agatggatat gccacaaacc attgcaattg 4800 ataccatccc agagcaaggt gcaacacatg atatgtgggg gaaaaatggt ggtgaatcac 4860 tttggggagg accagattta cctgattacc aactaaatat cgcatctaat atgttccatt 4920 caggctatat ttctagagca gttgatggtg tatttcctgt tgacgatgga ggaataaatt 4980 ataaaacggg tgaggaagca ggaattacag taaagttttc taaaggtaaa gggtattcaa 5040 cattgtgggg ggtgttagat gctgatgatg cagttgatcc tagtaaactt gtgaacttca 5100 ccgagcttgc ccatgattca acacgagata ctttttatga agcaaagatt cctttagctg 5160 caattggtaa tcccgacatt gaaaatgaac gcattggtgt catgattcat caaggtgaat 5220 tttcgccgat ggacacgcta ccgaatgacc ccgcaacatc cgatacacca ggtgtgagtg 5280 aatcaaattc gccattagaa tgggaagaca ttgacctgtt aacagtgcca tttgcaagaa 5340 ttggccaata attatgaaat aagccggcat gagtctatgc tggctttttg tacggctggc 5400 agttgcacat gcagagacga cactgtggtg taacagctag atgacagtaa atcattggca 5460 ttccagatgg tcggaataaa agacggattt gtgtatatag taactctatt gatgaagttt 5520 tcccgttagt tccatcctca gattatcaat catcaataat aggtggtttc atggattgct 5580 taggaaatat cgaggatgac ctatttactg ttcactaaat ctgattagag tttattggta 5640 tcaaaaaagc gttatttctt caaagcaaga agcaggcaac tgggattatc tacatttccc 5700 aatgatatga aaaatgctgt taaaaaagat ggttgaatat t 5741 // |
|