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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X53032; SV 1; linear; genomic DNA; STD; PRO; 1459 BP.
XX
AC   X53032;
XX
DT   16-JUL-1990 (Rel. 24, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 5)
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DE   Bacillus BamHI proviral methylase gene for BamHI proviral methylase
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KW   DNA-binding protein; methylase; provirus; provirus H2.
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OS   Bacillus amyloliquefaciens
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
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RN   [1]
RP   1-1459
RA   Connaughton J.F.;
RT   ;
RL   Submitted (04-MAY-1990) to the EMBL/GenBank/DDBJ databases.
RL   Connaughton J.F., Georgetown University Medical School, Dept. of
RL   Biochemistry & Molecular Biology, 4000 Reservoir Rd. N.W., Washington D.C.
RL   20007, USA.
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RN   [2]
RP   1-1459
RX   DOI; 10.1093/nar/18.13.4002
RX   PUBMED; 2374727.
RA   Connaughton J.F., Kaloss W.D., Vanek P.G., Nardone G.A., Chirikjian J.G.;
RT   "The complete sequence of the Bacillus amyloliquefaciens proviral H2, BamHI
RT   methylase gene";
RL   Nucleic Acids Res. 18(13):4002-4002(1990).
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CC   Wilson et al (wilson@neb.com) reports a new name for the gene and
CC   the protein : h2IIM for H2II methyltransferase. He proposes a
CC   start codon at bp 399 and a putative RBS at bp 385-391.
CC   Wilson G.G., "Amino acid sequence arrangements of DNA
CC   methyltransferases", Methods in Enzymology, Vol.216:259-279 (1992)
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FH   Key             Location/Qualifiers
FH
FT   source          1..1459
FT                   /organism="Bacillus amyloliquefaciens"
FT                   /strain="H"
FT                   /mol_type="genomic DNA"
FT                   /clone="pBamM6.5, pBamM2.5"
FT                   /db_xref="taxon:1390"
FT   RBS             423..427
FT                   /note="ribosome binding site"
FT   CDS             441..1238
FT                   /transl_table=11
FT                   /note="methylase (AA 1-265)"
FT                   /db_xref="GOA:P18051"
FT                   /db_xref="InterPro:IPR001091"
FT                   /db_xref="InterPro:IPR002941"
FT                   /db_xref="InterPro:IPR017985"
FT                   /db_xref="UniProtKB/Swiss-Prot:P18051"
FT                   /protein_id="CAA37205.1"
FT                   /translation="MKENIGDCTIDLTVTSPPYDDLRNYNGYSFNFEETAQELYRVTKE
FT                   GGVVVWVVGDKTHKGSETGSSFRQALYFKELGFNLHDTMIYEKDSISFPDKNRYYQIFE
FT                   YMFIFSKGKPKTINLLADRKNKWYNGKKHIKGHYRKMDGEKVRHHKQNLLKEFGVRFNI
FT                   WRIPNGHQKSTLDKIAFQHPAIFPEKLAEDHILSWSNEGDIVFDPFMGSGTTAKMAALN
FT                   NRKYIGTEISKEYCDIANERLKNYIILHKRMEGKGYRLPQVHS"
XX
SQ   Sequence 1459 BP; 547 A; 192 C; 308 G; 412 T; 0 other;
     aagcttattg aggaaacacc aagatcaata attgaaattt tagagtttat gactggtcaa        60
     agagatgaac atccaagtga gttgccagaa cataatctgt tttctgaaga tactagatgg       120
     aaatggatgc ttcaatcaga tagttattat ttcgatggga aaacccatag caaaattgaa       180
     aatgacgctg ttgttggtgg ttgttatgta agtattagat gtaatttaaa aaactacgat       240
     gatgagattg aaaaattcat cgactggatt tcatcatata ttcaaaagga ctacgatcat       300
     tattttattg gatacgaacg atatgaagag gacaaggagc ctacattaat atttgtgtaa       360
     aaggattatt tcatctgaaa actaaaggag ggtatttatt ggaactaaac aaaatacata       420
     ataatgattg cgtacaattc atgaaagaga atattggtga ttgcactatc gacttaacag       480
     tgacttctcc tccttatgat gacctaagga attacaatgg atattcattt aactttgagg       540
     aaactgctca agaattgtat agggtgacaa aagagggcgg agtcgttgtt tgggtagtag       600
     gggataaaac acataaaggt tccgagacag ggtcaagttt tagacaagct ttatacttta       660
     aagagttggg ctttaaccta catgacacaa tgatttatga aaaagacagt atcagctttc       720
     cagataagaa tagatattat cagatttttg agtatatgtt tattttttcg aaaggcaagc       780
     caaaaacaat caatctatta gcagacagga aaaataaatg gtacaacggc aagaaacaca       840
     taaaaggaca ttacagaaaa atggacggcg aaaaagtacg acatcataaa cagaacttac       900
     taaaggagtt cggtgtgagg ttcaacattt ggaggatacc gaacggtcat caaaaatcaa       960
     cactagacaa gatcgctttt caacatccgg caatatttcc agaaaagctt gctgaagatc      1020
     acattctctc ttggtcaaat gaaggagaca tagtatttga tccgtttatg ggaagtggaa      1080
     caactgcaaa gatggcagct ttaaataacc gtaaatacat aggaacagaa attagtaaag      1140
     agtattgtga tattgcaaat gaacgattaa agaattatat aattttgcat aaaagaatgg      1200
     agggcaaagg gtatcgatta cctcaagtac atagttgata cagcctggtt taacttagtt      1260
     tggtttaaat ggcatcttgg agcggatata agcatatttg atggctgtgg atggaataca      1320
     tataaatatt taaaggataa aaataaaata agtggaggtt atgaggggga taaaaaggaa      1380
     tctgcttagc aaccacgttg atgaggatta ttggtgaata ttacgcttca aaagggtatt      1440
     cagttcatag cattgaaca                                                   1459
//
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