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EBI DbfetchID X52988; SV 1; linear; genomic DNA; STD; PRO; 2350 BP. XX AC X52988; XX DT 13-AUG-1990 (Rel. 25, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 7) XX DE Bacillus amyloliquefaciens sacB gene for levansucrase (EC 2.4.1.10) XX KW levansucrase; sacB gene. XX OS Bacillus amyloliquefaciens OC Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus. XX RN [1] RP 1-2350 RA Vasantha N.; RT ; RL Submitted (09-MAY-1990) to the EMBL/GenBank/DDBJ databases. RL Vasantha N., E.I. du Pont, E228 IRm 310C, P.O.Box 80228 Wilmington,, DE RL 19880-0228, USA. XX RN [2] RP 1-2350 RX DOI; 10.1016/0378-1119(90)90345-R RX PUBMED; 2265762. RA Tang L.B., Lenstra R., Borchert T.V., Nagarajan V.; RT "Isolation and characterization of levansucrase-encoding gene from Bacillus RT amyloliquefaciens"; RL Gene 96(1):89-93(1990). XX FH Key Location/Qualifiers FH FT source 1..2350 FT /organism="Bacillus amyloliquefaciens" FT /strain="ATCC 23844" FT /mol_type="genomic DNA" FT /db_xref="taxon:1390" FT CDS 863..2281 FT /transl_table=11 FT /note="precursor (AA -29 to 443)" FT /db_xref="GOA:P21130" FT /db_xref="InterPro:IPR003469" FT /db_xref="UniProtKB/Swiss-Prot:P21130" FT /protein_id="CAA37179.1" FT /translation="MNIKKIVKQATVLTFTTALLAGGATQAFAKENNQKAYKETYGVSH FT ITRHDMLQIPKQQQNEKYQVPQFDQSTIKNIESAKGLDVWDSWPLQNADGTVAEYNGYH FT VVFALAGSPKDADDTSIYMFYQKVGDNSIDSWKNAGRVFKDSDKFDANDPILKDQTQEW FT SGSATFTSDGKIRLFYTDYSGKHYGKQSLTTAQVNVSKSDDTLKINGVEDHKTIFDGDG FT KTYQNVQQFIDEGNYTSGDNHTLRDPHYVEDKGHKYLVFEANTGTENGYQGEESLFNKA FT YYGGGTNFFRKESQKLQQSAKKRDAELANGALGIIELNNDYTLKKVMKPLITSNTVTDE FT IERANVFKMNGKWYLFTDSRGSKMTIDGINSNDIYMLGYVSNSLTGPYKPLNKTGLVLQ FT MGLDPNDVTFTYSHFAVPQAKGNNVVITSYMTNRGFFEDKKATFGPSFLMNIKGNKTSV FT VKNSILEQGQLTVN" FT sig_peptide 863..949 FT /note="signal peptide (AA -29 to -1)" FT mat_peptide 950..2278 FT /note="mature levansucrase (AA 1-443)" XX SQ Sequence 2350 BP; 739 A; 524 C; 506 G; 581 T; 0 other; gaattccttc aggaaaagaa cgatggctgt cttattagcg gttgcaggca catttatttt 60 ggtcacacac gggaatgtcg gcagcctgtc tatatccggt ctggctgttt tttggggcat 120 cagctcggca tttgcgctgg cgttttacac cctccagccg catcggcttt tgaagaaatg 180 gggctccgcc attattgtcg gatggggcat gctgatgcgg agccgttctc agcctgattc 240 agccgccttg gaagtttgaa ggccaatggt cgttgtccgc atatgccgcg atcgtgttta 300 tcatcatttt cggaacgctc atcgcttttt attgctattt ggaaagcctg aaatatctga 360 gtgcctctga aaccagcctc ctcgcctgtg cagagccgct gtcagcagct tttttagcgg 420 tgatctggct gcatgttccc ttcggaatat cagaatggct gggtacttta ctgattttag 480 ccaccatcgc tttattatct atcaagaaaa aataacctct cttttttaga gaggtttttc 540 cctaggcctg aagcaccctt tagtctcaat tacccataaa ttaaaaggcc ttttttcgtt 600 ttactatcat tcaaaagagg aaaatagacc agttgtcaat agaatcagag tctaatagaa 660 tgaggtcgaa aagtaaatca cgcaggattg ttactgataa agcaggcaag acctaaaatg 720 tgttaagggc aaagtgtatt ctttggcgtc atcccttaca tattttgggt ctttttttct 780 gtaacaaacc tgccatccat gaattcggga ggatcgaaac ggcagatcgc aaaaaacagt 840 acatacagaa ggagacatga acatgaacat caaaaaaatt gtaaaacaag ccacagttct 900 gacttttacg actgcacttc tggcaggagg agcgactcaa gccttcgcga aagaaaataa 960 ccaaaaagca tacaaagaaa cgtacggcgt ctctcatatt acacgccatg atatgctgca 1020 gatccctaaa cagcagcaaa acgaaaaata ccaagtgcct caattcgatc aatcaacgat 1080 taaaaatatt gagtctgcaa aaggacttga tgtgtgggac agctggccgc tgcaaaacgc 1140 tgacggaaca gtagctgaat acaacggcta tcacgttgtg tttgctcttg cgggaagccc 1200 gaaagacgct gatgacacat caatctacat gttttatcaa aaggtcggcg acaactcaat 1260 cgacagctgg aaaaacgcgg gccgtgtctt taaagacagc gataagttcg acgccaacga 1320 tccgatcctg aaagatcaga cgcaagaatg gtccggttct gcaaccttta catctgacgg 1380 aaaaatccgt ttattctaca ctgactattc cggtaaacat tacggcaaac aaagcctgac 1440 aacagcgcag gtaaatgtgt caaaatctga tgacacactc aaaatcaacg gagtggaaga 1500 tcacaaaacg atttttgacg gagacggaaa aacatatcag aacgttcagc agtttatcga 1560 tgaaggcaat tatacatccg gcgacaacca tacgctgaga gaccctcact acgttgaaga 1620 caaaggccat aaataccttg tattcgaagc caacacggga acagaaaacg gataccaagg 1680 cgaagaatct ttatttaaca aagcgtacta cggcggcggc acgaacttct tccgtaaaga 1740 aagccagaag cttcagcaga gcgctaaaaa acgcgatgct gagttagcga acggcgccct 1800 cggtatcata gagttaaata atgattacac attgaaaaaa gtaatgaagc cgctgatcac 1860 ttcaaacacg gtaactgatg aaatcgagcg cgcgaatgtt ttcaaaatga acggcaaatg 1920 gtacttgttc actgattcac gcggttcaaa aatgacgatc gatggtatta actcaaacga 1980 tatttacatg cttggttatg tatcaaactc tttaaccggc ccttacaagc cgctgaacaa 2040 aacagggctt gtgctgcaaa tgggtcttga tccaaacgat gtgacattca cttactctca 2100 cttcgcagtg ccgcaagcca aaggcaacaa tgtggttatc acaagctaca tgacaaacag 2160 aggcttcttc gaggataaaa aggcaacatt tggcccaagc ttcttaatga acatcaaagg 2220 caataaaaca tccgttgtca aaaacagcat cctggagcaa ggacagctga cagtcaacta 2280 ataacagcaa aaagaaaatg ccgatacttc attggcattt tcttttattt ctcaacaaga 2340 tggtgaattc 2350 // |
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