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EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X52944; SV 1; linear; genomic DNA; STD; PRO; 1618 BP.
XX
AC   X52944;
XX
DT   31-JUL-1990 (Rel. 25, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 4)
XX
DE   Erwinia carotovora peh gene for endo-polygalacturonase (EC 3.2.1.15)
XX
KW   endo-polygalacturonase; pectolytic enzyme.
XX
OS   Pectobacterium carotovorum
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
OC   Enterobacteriaceae; Pectobacterium.
XX
RN   [1]
RP   1-1618
RA   Hinton J.C.D.;
RT   ;
RL   Submitted (09-FEB-1990) to the EMBL/GenBank/DDBJ databases.
RL   Hinton J.C.D., Imperial Cancer Research Fund laboratories, Institute of
RL   Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford.
RL   OX3 9DU. UK.
XX
RN   [2]
RP   1-1618
RX   DOI; 10.1111/j.1365-2958.1990.tb00675.x
RX   PUBMED; 2215211.
RA   Hinton J.C.D., Gill D.R., Lalo D., Plastow G.S., Salmond G.P.C.;
RT   "Sequence of the peh gene of Erwinia carotovora: homology between Erwinia
RT   and plant enzymes";
RL   Mol. Microbiol. 4(6):1029-1036(1990).
XX
CC   Data kindly reviewed (15-AUG-1990) by Hinton J.C.D.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1618
FT                   /organism="Pectobacterium carotovorum"
FT                   /strain="SCRI193, sub-species carotovora"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="pUC8"
FT                   /clone="pHHX"
FT                   /db_xref="taxon:554"
FT   misc_feature    138..175
FT                   /note="sigma-70-type promoter"
FT   misc_feature    141..159
FT                   /note="KdgR-binding site"
FT   RBS             281..284
FT                   /note="ribosome binding site"
FT   CDS             291..1499
FT                   /transl_table=11
FT                   /product="precursor protein (AA -26 to 376)"
FT                   /db_xref="GOA:P18192"
FT                   /db_xref="HSSP:1BHE"
FT                   /db_xref="InterPro:IPR012334"
FT                   /db_xref="UniProtKB/Swiss-Prot:P18192"
FT                   /protein_id="CAA37119.1"
FT                   /translation="MEYQSGKRVLSLSLGLIGLFSASAFASDSRTVSEPKAPSSCTVLK
FT                   ADSSTATSTIQKALNNCGQGKAVKLSAGSSSVFLSGPLSLPSGVSLLIDKGVTLRAVNN
FT                   AKSFENAPSSCGVVDTNGKGCDAFITATSTTNSGIYGPGTIDGQGGVKLQDKKVSWWDL
FT                   AADAKVKKLKQNTPRLIQINKSKNFTLYNVSLINSPNFHVVFSDGDGFTAWKTTIKTPS
FT                   TARNTDGIDPMSSKNITIAHSNISTGDDNVAIKAYKGRSETRNISILHNEFGTGHGMSI
FT                   GSETMGVYNVTVDDLIMTGTTNGLRIKSDKSAAGVVNGVRYSNVVMKNVAKPIVIDTVY
FT                   EKKEGSNVPDWSDITFKDITSQTKGVVVLNGENAKKPIEVTMKNVKLTSDSTWQIKNVT
FT                   VKK"
FT   sig_peptide     291..368
FT                   /note="signal peptide (-26 to -1)"
FT   mat_peptide     369..1496
FT                   /note="endo-polygalacturonase (AA 1-376)"
XX
SQ   Sequence 1618 BP; 467 A; 317 C; 415 G; 419 T; 0 other;
     aagcttacca cccgctggct tctgcgcatg acgggattcg ttttgtttcc acgtttcgtt        60
     gatacttcat ttaatttttt ggtgatattt taaccatgcg taaagttgaa tgctttccga       120
     ataagcattg gaataatttt atgtaaatgg acttttcatt aatctacata ggcatgtgtg       180
     acaattttta gtttctataa aggtgtttta tttttcttat taagcaaaat gaaagcgtaa       240
     tgccttttgg ggatggaaaa gttgtggatg cttataaggt aaggtttgac atggaatatc       300
     aatcaggcaa gcgagtttta tcattatcac tggggcttat cggtttgttt agcgcatcgg       360
     catttgcttc tgattcccga acggtgagtg aaccgaaagc accgtcttcc tgtacggtgc       420
     ttaaagctga cagtagtacg gccacgagta caattcaaaa agcgctgaat aattgcgggc       480
     aaggaaaagc ggtaaagctg agcgcaggga gttcatccgt ttttctgagc ggtccgcttt       540
     ctctaccttc tggcgtgagc ttattaatcg acaaaggggt aaccctacgt gctgtgaata       600
     atgccaagtc ttttgaaaat gcgccctcat cctgtggcgt ggtggataca aacggtaaag       660
     gctgcgatgc gtttattacc gccacaagca cgacgaatag cggaatctat gggccgggca       720
     ccattgatgg acaagggggc gtgaagcttc aggataaaaa ggtgagctgg tgggatctgg       780
     ctgccgatgc caaagtgaaa aagttaaaac agaatacccc ccggctgatt cagattaata       840
     agagcaagaa cttcacgctg tataacgttt ctctcattaa ttccccgaat ttccacgtcg       900
     tgttcagcga tggtgacggc ttcaccgcgt ggaaaaccac gatcaaaacg ccatctaccg       960
     ccagaaacac cgacggtatc gatcctatgt cgtcaaagaa catcaccatt gcccacagta      1020
     atatttcgac gggtgacgat aatgtggcga tcaaagccta taaggggcgt tctgagacac      1080
     gcaatatttc cattttgcat aatgaatttg gaacgggaca tggcatgtcg atcggtagcg      1140
     aaacgatggg agtttataac gtgacggtcg atgatctgat tatgactggc accacgaatg      1200
     gcttacgtat taaaagcgac aaatcagcag ctggcgttgt caatggtgtt cggtatagca      1260
     acgtagtcat gaagaacgtg gcgaaaccga tcgtgattga cacggtatat gagaaaaaag      1320
     agggaagtaa tgttcctgac tggagcgaca ttacgtttaa ggatattacg tctcaaacca      1380
     aaggcgtggt ggtgctgaac ggcgagaatg cgaaaaagcc gatagaagtg acgatgaaga      1440
     acgtcaaact gacgagcgac agcacatggc aaatcaagaa cgtcaccgtt aagaagtagt      1500
     tcgattaata agttgttttg tcatgacaac gtccagagcg ttgacaaatc tacttgcaga      1560
     acgagaacgg tggtaatggg atagaagagt aaagcgccca gggatggcgc ggctcgag        1618
//
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