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EBI DbfetchID X52176; SV 1; linear; genomic DNA; STD; PRO; 3987 BP. XX AC X52176; XX DT 25-MAY-1990 (Rel. 24, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 8) XX DE Leptospira interrogans sphingomyelinase-c gene (EC 3.1.4.12) XX KW hemolysin; sphingomyelinase; sphingomyelinase-c; KW unidentified reading frame. XX OS Leptospira interrogans OC Bacteria; Spirochaetes; Spirochaetales; Leptospiraceae; Leptospira. XX RN [1] RP 1-3987 RA Segers R.P.A.M.; RT ; RL Submitted (30-MAR-1990) to the EMBL/GenBank/DDBJ databases. RL Segers R.P.A.M., Institute of Infectious Diseases and Immunology, RL Department of Bacteriology, Faculty of Veterinary Medicine, State RL University of Utrecht, P.O. Box 80.165, 3508 TD Utrecht, The Netherlands. XX RN [2] RP 1-3987 RA Segers R.P.A.M., Van der Drift A., De Nijs A., Corcione P., RA Van der Zeijst B.A.M., Gaastra W.; RT "Molecular analysis of a sphingomyelinase-c gene from Leptospira RT interrogans serovar hardjo"; RL Infect. Immun. 50:2177-2185(1990). XX FH Key Location/Qualifiers FH FT source 1..3987 FT /organism="Leptospira interrogans" FT /strain="sponselee, serogroup sejroe, serovar hardjo" FT /mol_type="genomic DNA" FT /clone_lib="lambda EMBL3" FT /clone="pHL2-B3, pHL2-B4" FT /db_xref="taxon:173" FT promoter 528..533 FT /note="pot. -35 region" FT promoter 559..564 FT /note="pot. -10 region" FT RBS 570..574 FT /note="pot. ribosome binding site" FT CDS 581..2251 FT /transl_table=11 FT /note="precursor protein (AA -27 to 529)" FT /db_xref="GOA:P17627" FT /db_xref="InterPro:IPR005135" FT /db_xref="InterPro:IPR017766" FT /db_xref="UniProtKB/Swiss-Prot:P17627" FT /protein_id="CAA36424.1" FT /translation="MRIKKYTKVRLLVNCCLLLFFLIDCGADRQSLYKDLLASLIYISD FT NKNIGSTNSDLTGSGSVSSSPADAAPENSILANSIPENMGIKILTHNVFLLPKTLPGWG FT NWGQNERAQRIVSSNYIQNQDVIVFDEAFDTDARKILLDGVRSEYPYQTDVIGRTKKGW FT DATLGLYRTDAFTNGGVVIVSKWPIEEKIQHVFKEKGCGADVFSNKGFAYVRIDKNGRK FT FHIIGTHVQAQDSGCANLGVVSRVNQFNEIRDFIDSKKIPKNEMVLIAGDLNVIKGSRE FT YHQMLCILNVNNPKYVGVPFTWDTKTNEIAAFYYKKVEPAYLDYIFVSKSHFQPPIWQN FT LAYDPISAKTWTAKGYTSDEFSDHYPVYGFIYADSSTPTKSGRKRKYDRVSFVSVATGK FT KIQANSEKSNAWLKVNATTETDLTKFNLVQTNDPDSNPSCMKSGHVRIESSHSLNYFWN FT WWLGGGKGNYAYYPKFNDGSNRIQIINLDGGCLQDGSRVAFKDYDTISRRQYFLTVWEG FT GNWDKYLYLWRSHIGLREIFYLKLDSSPEMNWSKKLIYR" FT sig_peptide 581..661 FT /note="signal peptide (AA -27 to -1)" FT mat_peptide 662..2248 FT /note="mature sphingomyelinase-c (AA 1-529)" FT repeat_region 1337..1365 FT /note="inverted repeat A" FT repeat_region 1632..1755 FT /note="inverted repeat C" FT repeat_region 1698..1724 FT /note="inverted repeat B" FT repeat_region 2268..2297 FT /note="inverted repeat D" FT repeat_region 2315..2347 FT /note="inverted repeat E" FT promoter complement(3277..3281) FT /note="pot. -10 region" FT repeat_region 3332..3370 FT /note="inverted repeat F" FT CDS complement(2434..3345) FT /transl_table=11 FT /note="ORF 1 (AA 1-303)" FT /db_xref="UniProtKB/Swiss-Prot:P17628" FT /protein_id="CAA36425.1" FT /translation="MEIFCICLLLDPIWIFGDTLILKDGRKMGNVRTSLREDHVLVEDE FT TGKVEKIDLTLVEKILVSEIKKPEGEEKQKEHNNIKKFYFSWNLSSWSTKVTEKMNFNR FT GYNITDLITGVIYIDPYIEKRYTVNTKTTSFNGEYRYNLNLSFLFGIELNSYSFPDRKI FT SPLVGILMNSNFNSTPEYQTLSSISINLFLFEGNFNFDKQGNDKFGIETLSLLPGMKYY FT FPLSESIFWFTQVGLGVGKSIESGVHSKVQTVLFVGTGIQWELESYFFNIALQYRKTDL FT IGATQSYHFNEPIFMIGGGLKL" FT RBS complement(3354..3357) FT /note="pot. ribosome binding site" FT promoter complement(3394..3399) FT /note="pot. -35 region" FT CDS complement(3396..>3987) FT /codon_start=2 FT /transl_table=11 FT /note="ORF 2 (incomplete) (197 AA) (3987 is 3rd base in FT codon)" FT /db_xref="UniProtKB/Swiss-Prot:P24845" FT /protein_id="CAA36426.1" FT /translation="DPQKEELIEHSLNKQTGSNFGQKTELEKVWVDTSSEDISNSYFEI FT KPLNLSGSREKSLFKTLKEDRLDAKSAQENLNILKEIQQQIIKNVSQKKQAPLNELYLK FT DLENISNQLGNSEPIKLSEIEKKQEPIERKTSTTTNTESNQEKPLRLGNKTIQLKDGTI FT LKGNAMQYGSQYILEENEKKRIIESTNIESISF" XX SQ Sequence 3987 BP; 1205 A; 685 C; 746 G; 1351 T; 0 other; ggatcctttt ttcgttattt ttactaagaa tttgttccaa ggacgtggaa acttttacgt 60 tttagatgtt cgtggataga acttttttgc tcctaatttt gttcatcaag tagcttgatc 120 tttgtaaatc tgatttgaga actttttaca ttacatacaa gtccataaaa cgccatatat 180 gcttgaagtc gatttctaat ttcttgtgga acgatttttg aaatgatgaa atatatatta 240 tatttttaat ataatatagc ttataaaagc cgtttaatct gcccggtgtg ggatttcaaa 300 atcataaaag attagaaatc gtttttatat gacatacgat tatcaaattt cttcatttaa 360 tcttgaaaca tgatctaaaa agtaaacaaa gactttgttc gcttttaatt tgtttatttt 420 tatacattta aaattttgaa ggttgcttta ggaattggca tttttaattt caattacttg 480 agcgttatat gaaattgatt cgtttttata aaatactctt cttcttgttg aactctatgt 540 tatagtatgg gcattgattt aaaaagaatg gagatagtgt atgagaataa aaaaatatac 600 aaaagtgaga cttcttgtaa attgttgtct cttattattt tttctaatag attgtggagc 660 cgatagacaa tctttgtata aagatttact tgcgtctcta atttacattt cagataataa 720 aaacattggt tctacaaatt ctgatttaac gggttctggc tctgtaagtt ctagtcccgc 780 agatgccgct cctgagaatt ccatccttgc gaattctatt cctgagaaca tgggaattaa 840 aattctaact cacaacgttt tcctgttgcc gaaaacgctt ccaggttggg gaaattgggg 900 acaaaacgaa agagcacaac gtatcgtgag ttcgaattat atccaaaacc aagacgtcat 960 tgtgttcgat gaagcttttg atacagatgc aagaaaaatt ctcttggacg gagttcgttc 1020 agaatatccg tatcaaaccg atgtgatcgg aagaactaaa aaaggttggg atgctactct 1080 tggcttgtac agaacggatg cgtttacgaa cggaggagtt gttattgtaa gtaaatggcc 1140 catcgaagag aaaatacaac atgtcttcaa agaaaagggc tgtggcgcgg atgttttttc 1200 caacaaagga tttgcttatg tgaggatcga taaaaacgga agaaagtttc atatcattgg 1260 aactcatgta caggcacaag attccggatg tgcgaactta ggagtcgtct caagagtgaa 1320 tcaattcaac gagatcaggg attttatcga ttccaaaaaa atcccgaaaa acgaaatggt 1380 tctgatcgcg ggagatttga acgtaatcaa aggtagtaga gaatatcatc agatgctttg 1440 tatattgaac gtaaacaatc ctaaatatgt aggagttcct tttacgtggg atacaaaaac 1500 caatgaaatt gccgcttttt attataaaaa agtggagccc gcatacttgg attacatatt 1560 cgtttcaaag tcccactttc aaccaccgat ttggcagaat ttagcttacg atccaatctc 1620 tgcaaaaact tggaccgcaa agggatatac gagtgatgaa ttttccgatc actatcccgt 1680 ttacggtttt atctatgcgg attcatccac tccgacgaag tccggacgta aaaggaaata 1740 cgatcgagtt tccttcgtat ctgtagccac tggaaaaaag attcaagcta attctgaaaa 1800 atcgaacgca tggttgaagg tgaatgctac gacggaaacc gatttaacaa aattcaattt 1860 ggtgcaaacg aatgatcccg attcgaatcc ctcctgcatg aaaagcgggc atgtccggat 1920 cgaatcttct cattctttaa attacttttg gaattggtgg ctcggaggag gtaaaggtaa 1980 ctacgcgtac tatcccaaat tcaacgacgg gtcgaatcga atccaaatca taaacttaga 2040 cggagggtgt ttgcaagacg ggagtcgagt cgcattcaaa gattatgata ctatttcaag 2100 aagacaatat tttctcactg tttgggaagg tggaaactgg gataaatatc tctatctttg 2160 gagaagccat atcggtctaa gggaaatttt ttatctcaag ctggattcct ctcccgaaat 2220 gaattggagt aagaaattaa tttatcgttg agccttaaaa atcaaatgaa agacggcagg 2280 gatgtccctg cctcttttcg cgtacataga atttttatat cgatccagga ctttgcgata 2340 gatataatga ggctctaatt ctaattttat tactcggacg tatgaaaaaa ttccatacag 2400 gaattagtgg taaaagaagc cttcggtttg atcttacagc ttcaaccctc cgccgatcat 2460 aaagatcggt tcgttaaaat gataagattg tgtcgcccca attaaatctg tttttcggta 2520 ttgtagagct atattaaaaa agtaagattc tagttcccat tgaatgcccg ttcccacaaa 2580 aaggaccgtt tgtactttgg aatgtactcc actttcaata ctttttccga ctcccaagcc 2640 tacttgagtg aaccaaaaaa tagactcgga aagaggaaaa taatatttca ttcccggcaa 2700 aagtgaaaga gtttctatac cgaatttatc gttcccttgt ttatcaaaat taaaatttcc 2760 ttcaaacaga aataaattta tggaaatgct ggataacgtt tggtattccg gggtagagtt 2820 aaaattagag ttcataagaa ttccaacaag aggagaaatc ttgcgatccg gaaaagaata 2880 agagtttagt tcgattccga ataaaaagct gagattcaaa ttgtaacgat attcgccatt 2940 aaaggaagtt gttttggtat ttacagtata tcttttctca atataagggt ctatatagat 3000 tacgcccgtg ataaggtccg tgatgttgta tccgcggttg aaattcatct tttccgtcac 3060 tttggtgctc caacttgaca aattccaaga aaaatagaat tttttaatat tattatgttc 3120 tttttgtttc tcttcgcctt ccggcttttt gatttccgaa acaagaattt tttcaaccag 3180 agttaaatcg attttttcga cttttcccgt ttcgtcctcg actaagacgt gatcctcccg 3240 cagtgaagtt ctaacgtttc ccatttttct tccgtctttt agaatcaaag tgtctccaaa 3300 aatccagatc ggatctaata ggaggcatat acaaaaaatt tccatgagcg aaactcccgg 3360 aaactttttg aaaaagtgat ttgagtttag gatcttcaaa atgaaatcga ctctatattt 3420 gtggattcga tgattctttt tttttcgttt tcttccaaga tatattggct tccgtattgc 3480 attgcatttc ctttaagtat cgttccgtct ttgagttgaa tcgttttatt tcctaaacgc 3540 aaaggttttt cttggttgct ttcggtattt gtggtggtcg aagtttttct ttcgatcggt 3600 tcctgttttt tctctatctc cgagagtttt ataggttcag aatttccaag ttgatttgaa 3660 atattttcta gatctttgag atacaactca ttcaacggag cttgtttttt ttgcgagacg 3720 ttctttatga tttgttgttg aatctctttt agaatattca aattttcttg tgcgcttttt 3780 gcatcaagtc gatcttcttt caaagtctta aataaacttt tttcccgact tcccgataga 3840 tttagaggtt ttatttcaaa ataagaatta gagatatctt ccgaagaagt gtccacccat 3900 actttttcca attcggtctt ttgcccgaag tttgaacccg tttgtttatt caaagaatgt 3960 tctatcaact cttccttttg gggatcc 3987 // |
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