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EBI DbfetchID X52124; SV 1; linear; genomic DNA; STD; PRO; 2825 BP. XX AC X52124; XX DT 13-JUL-1990 (Rel. 24, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Haemophilus influenzae genes for HincII restriction-modification system DE (HincII methyltransferase (EC 2.1.1.72) and HincII endonuclease (EC DE 3.1.21.4)) XX KW endonuclease; HincII endonuclease; HincII methyltransferase; KW methyltransferase; nuclease; restriction modification enzyme; transferase. XX OS Haemophilus influenzae OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; OC Pasteurellaceae; Haemophilus. XX RN [1] RP 1-2825 RA Ito H.; RT ; RL Submitted (23-MAR-1990) to the EMBL/GenBank/DDBJ databases. RL Ito H., Institution Bioproducts Development Center, Takara Shuzo, Co., RL Ltd., Seta 3-4-1, Otsu, Shiga 520-21, Japan. XX RN [2] RP 1-2825 RX DOI; 10.1093/nar/18.13.3903 RX PUBMED; 2374714. RA Ito H., Sadaoka A., Kotani H., Hiraoka N., Nakamura T.; RT "Cloning, nucleotide sequence, and expression of the HincII RT restriction-modification system"; RL Nucleic Acids Res. 18(13):3903-3911(1990). XX CC Data kindly reviewed (10-SEP-1990) by Ito H. XX FH Key Location/Qualifiers FH FT source 1..2825 FT /organism="Haemophilus influenzae" FT /strain="Rc" FT /mol_type="genomic DNA" FT /clone_lib="pBR322" FT /clone="pAH2 plasmid" FT /db_xref="taxon:727" FT misc_feature 462..491 FT /note="stem loop structure" FT CDS 516..2024 FT /transl_table=11 FT /note="HincII methyltransferase (AA 1-502)" FT /db_xref="GOA:P17744" FT /db_xref="InterPro:IPR002052" FT /db_xref="InterPro:IPR002296" FT /db_xref="InterPro:IPR003356" FT /db_xref="UniProtKB/Swiss-Prot:P17744" FT /protein_id="CAA36369.1" FT /translation="MDESKKISLGQFFTPTHIVKYMIGLMTKNKNASILEPSSGNGVFL FT DSLIQLGYTNLTSYEIDGDIISHPFVINSSFITSYDKPQYDSIIGNPPYVRWKNLSELQ FT KKELKDNSIWKMYCNSLCDYFYIFIIKSILQLKVGGELIFICPDYFFSTKNAEGLRKFL FT INNGSFEKIILFNESKVFHGVSSSVVIFKYIKGKNIDNINIINIDSKSPIKSEDIESLG FT ESYYIPRFSSSDVWVTSPNHIKVALDKFESYCKTIKKVQQKSLFDDLSFLDKAFQMNDI FT NYSELELLNSICVAKAKHLDAFCFSGYTRYKLILDDNNEDKLITYFPNFFYEFNNYKDY FT LLKRYSYNKYLPYWEVAFLRNFSLFSKNEKKIFVPCKERISKKSNFRFSLVDEFIYPTQ FT DVTALYKKENVKESIEYITAYLNSKAVFLWMKYKGVVKGNVVEFSEKPLTNIPFRRIDW FT QLKSEKKIHDDITNLVRKYLSNKEFSILHEINLNLEKLGIKVEI" FT CDS 2021..2797 FT /transl_table=11 FT /note="HincII endonuclease (AA 1-258)" FT /db_xref="GOA:P17743" FT /db_xref="InterPro:IPR011335" FT /db_xref="InterPro:IPR011337" FT /db_xref="InterPro:IPR015307" FT /db_xref="PDB:1KC6" FT /db_xref="PDB:1XHU" FT /db_xref="PDB:1XHV" FT /db_xref="UniProtKB/Swiss-Prot:P17743" FT /protein_id="CAA36370.1" FT /translation="MSFIKPIYQDINSILIGQKVKRPKSGTLSGHAAGEPFEKLVYKFL FT KENLSDLTFKQYEYLNDLFMKNPAIIGHEARYKLFNSPTLLFLLSRGKAATENWSIENL FT FEEKQNDTADILLVKDQFYELLDVKRRNISKSAQAPNIISAYKLAQTCAKMIDNKEFDL FT FDINYLEVDSELNGEDLVCVSTSFAELFKSEPSELYINWAAAMQIQFHVRDLDQGFNGT FT REEWAKSYLKHFVTQAEQRAISMIDKFVKPFKKYIL" XX SQ Sequence 2825 BP; 1045 A; 340 C; 487 G; 953 T; 0 other; aagcttacga ctctgaattc cttgagggag aacaagtcga agtggctcgc gtgaaaattg 60 ttaatcgcca acgtgaagca gaaggcaaac caccagttga atttgaacgt gaattattgg 120 gtatcaccaa agcgtctctt gcgactgaat ccttcatctc tgccgcatcg ttccaagaaa 180 ctactcgtgt tcttaccgaa gcagcagtgg caggtaaacg tgatgaatta cgtggcttga 240 aagagaacgt aatagtaggt cgattaattc ctgcgggtac aggttttgcg tatcatcaaa 300 atcgtcacaa acatcgctta gttgatgatg tcgtagcgaa attatctgaa gaagatgaag 360 ccgtatcgct gatgagtgtc ataacggcag atgatgcaac gcaaaatctt gcaacattgt 420 taaattctga aatagaagat taatatttgt atcttatgtt taaaccctca gaatgagagt 480 tctgagggtt tttattagaa aaataggata aaaatatgga tgaatcaaag aaaatatcac 540 taggtcagtt ttttactcct acacatattg ttaaatatat gattggacta atgacgaaaa 600 ataaaaatgc tagtatttta gaacctagta gtgggaatgg tgtatttcta gatagtttaa 660 ttcaattagg ttatacaaat ttaacatcgt atgaaataga tggtgatatt atttctcatc 720 catttgtaat taactcaagt tttataacat catatgataa gcctcaatat gattcgatca 780 ttggaaatcc tccttatgtt cgttggaaaa acctaagtga gcttcagaag aaagagctta 840 aagataattc tatatggaaa atgtattgta atagtctttg tgattatttc tatattttta 900 ttattaaatc tatacttcaa ttaaaagttg gaggagaatt aatatttatt tgccctgatt 960 attttttttc cactaagaac gctgagggtt taaggaaatt tttgataaat aatggaagtt 1020 ttgaaaaaat tatattattt aatgaaagta aagtatttca tggagtttct tcttcagttg 1080 ttatttttaa atatataaaa ggaaaaaata ttgataatat aaatattatt aatatagatt 1140 caaaaagccc aataaaatct gaggatatag aatctttagg tgaatcatat tatattccaa 1200 gattttctag ctcagatgtt tgggttactt cgcccaatca tattaaagtt gcattagata 1260 aatttgagag ttactgcaaa actatcaaaa aagttcagca aaaatcatta tttgatgatc 1320 ttagttttct agataaggct tttcagatga atgatataaa ttattcagaa ttagaattgc 1380 ttaattcaat ttgtgtagct aaagctaaac acttagacgc attttgtttc tctggataca 1440 ccagatacaa gttaatatta gatgataata atgaggataa attaatcact tattttccaa 1500 atttctttta tgagtttaat aattataaag attatctatt aaaaagatat agttataata 1560 aatatttgcc ttattgggaa gtggcttttc ttagaaattt ttctctattc agcaagaatg 1620 agaagaaaat atttgtgccg tgcaaagaaa gaatttcaaa aaaaagtaat tttagattta 1680 gcttagttga tgaatttata tatccaacac aggatgttac tgcattatat aagaaagaaa 1740 atgtaaagga gagtattgag tatattacag catatttaaa ctctaaagct gtgtttttat 1800 ggatgaagta taagggtgtt gtcaaaggta atgtagttga attttcagaa aaaccattaa 1860 caaatattcc ttttagaaga atagattggc aattaaaaag cgaaaaaaaa attcatgatg 1920 atataactaa tttagtaaga aaatatttat caaacaaaga attttcaatt ttacatgaga 1980 ttaacttaaa tttagaaaaa ttaggtataa aggtggaaat atgagtttca taaaacctat 2040 ttatcaggat attaattcaa tattaatcgg gcaaaaagtg aaacgtccta aatcaggtac 2100 tctgtcaggt catgctgcag gggaaccatt tgaaaaatta gtatataagt ttttgaaaga 2160 aaacctgtca gatttaacat ttaagcaata tgaatatctt aatgatttat ttatgaagaa 2220 ccctgcgata attggacatg aagctagata taaattattt aattctccaa cattgctttt 2280 tttgttaagt agaggtaaag ctgcaactga aaattggagc atagaaaatt tatttgagga 2340 gaaacaaaat gatactgcag atattttatt agtaaaggat cagttctatg aattgttaga 2400 tgtcaaaaga agaaatatta gtaaatcagc tcaagcaccc aatattattt cagcatataa 2460 attagctcag acgtgtgcaa aaatgattga taataaagaa tttgatttat ttgatattaa 2520 ttatttagaa gtagactcgg aacttaatgg tgaagatcta gtttgtgtat ctacttcttt 2580 tgctgaacta tttaaatctg aacctagtga actatatatt aactgggctg cagctatgca 2640 aattcagttt catgtaagag atttagatca ggggtttaat ggaactagag aagaatgggc 2700 aaaatcttat ctaaaacatt ttgttactca agcagagcaa agagccatat ctatgataga 2760 taagtttgtt aagccattta agaaatatat actttagttt caagttcaaa agatattata 2820 agctt 2825 // |
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