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EBI DbfetchID X17284; SV 1; linear; genomic DNA; STD; PRO; 3562 BP. XX AC X17284; XX DT 11-JUL-1990 (Rel. 24, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 5) XX DE Erwinia chrysanthemi pelE and pelD genes XX KW lyase; pectate lyase; pelD gene; pelE gene. XX OS Erwinia chrysanthemi OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Dickeya. XX RN [1] RP 1-3562 RA Van Gijsegem F.; RT ; RL Submitted (14-DEC-1989) to the EMBL/GenBank/DDBJ databases. RL Van Gijsegem F., Universite Libre de Bruxelles, Department de Biologie RL Moleculaire, Service de Genetique, rue des Chevaux, 67, B-1640 RL Rhode-Saint-Genese, Belgium. XX RN [2] RP 1-3562 RX DOI; 10.1111/j.1365-2958.1989.tb00124.x RX PUBMED; 2615652. RA Van Gijsegem F.; RT "Relationship between the pel genes of the pelADE cluster in Erwinia RT chrysanthemi strain B374"; RL Mol. Microbiol. 3(10):1415-1424(1989). XX FH Key Location/Qualifiers FH FT source 1..3562 FT /organism="Erwinia chrysanthemi" FT /strain="B374" FT /mol_type="genomic DNA" FT /db_xref="taxon:556" FT RBS 299..303 FT /note="ribosome binding site" FT CDS 311..1525 FT /transl_table=11 FT /product="pelE precursor (AA -41 to 363)" FT /db_xref="GOA:P0C1A4" FT /db_xref="HSSP:1JRG" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/Swiss-Prot:P0C1A4" FT /protein_id="CAA35175.1" FT /translation="MNNSRMSSVSTQKTTGRSALGTKSALAAIIATTMMVSVASAASLQ FT TTKATEAASTGWATQSGGTTGGAKASSSKIYAVKSISEFKAALNGTDSSPKIIQVTGAI FT DISGGKAYTSFDDQKARSQISIPSNTTIIGIGNKGKFTNGSLVVKGVSNVILRNLYIET FT PVDVAPHYEEGDGWNAEWDAVVIDSTDHVWVDHVTISDGSFTDDKYTTKNGEKYVQHDG FT SLDIKRGSDYVTVSNSRFELHDKTILIGHSDNNGSQDAGKLRVTFHNNLFDRVGERTPR FT VRFGSVHAYNNVYVGDVNHKAYRYQYSFGIGTSGSLLSESNAFTIDNMKKISGRDKECS FT VVKAFNGKIFSDKGSIINGASYNLNGCGFGFSAYSAKIPYKYSAQTITTSLAGSISSNA FT GYGKL" FT sig_peptide 311..433 FT /note="signal peptide (AA -41 to -1)" FT mat_peptide 434..1522 FT /note="pelE protein (AA 1-363)" FT terminator 1540..1570 FT /note="pelE transcription terminator" FT RBS 2033..2037 FT /note="ribosome binding site" FT CDS 2045..3220 FT /transl_table=11 FT /product="pelD" FT /db_xref="GOA:P18209" FT /db_xref="HSSP:1JRG" FT /db_xref="InterPro:IPR012334" FT /db_xref="UniProtKB/Swiss-Prot:P18209" FT /protein_id="CAA35176.1" FT /translation="MNNTRVSFRSTKSLLAAIIATSMMTWSVNRATLQTTKATEAASTG FT WATQGGTTGGAKAASAKIYAVKNISEFKAALNGTDTDPKIIQVTGAIDISGGKAYTSFD FT DQKARSQISVPSNTTIIGIGSNGKFTNGSLVIKGVSNVILRNLYIETPVDVAPHYEEGD FT GWNAEWDAAVIDNSTRVWVDHVTISDGSFTDDKYTTKNGEKYVQHDGALDIKKGSDYVT FT ISSSRFELHDKTILIGHSDSNGSQDSGKLRVTFHNNVFDRVTERTPRVRFGSIHAYNNV FT YLGDVKNSVYPYLYSFGLGTSGTILSESNSFTLSNLKSIDGKNPECSIVKQFNSKVFSD FT NGSLVNGSSTTKLDTCAVTAYKPTLPYKYSAQTMTSSLASSINSNAGYGKL" FT sig_peptide 2045..2137 FT /note="signal peptide (AA -31 to -1)" FT mat_peptide 2138..3217 FT /note="pelD protein (AA 1-360)" FT terminator 3242..3266 FT /note="pelD transcription terminator" XX SQ Sequence 3562 BP; 1073 A; 982 C; 776 G; 731 T; 0 other; aaaccgccag tctaaaacag cagaattaat taactatcac cgttacaatc atcgaaatta 60 aaaatataaa taattgattt aaatcataaa aaataaaaaa ccaagtaaca caaagttaca 120 aatacagtca atagtttatt ttttattaat aaacaccatt gtcatcatcg tgacaaagtt 180 cacaaaatag acactaaaac cgcataaaca ttcgccaaaa caaaagatga aatggtattt 240 cgtttttaga cacacatgta acaaatggac accatttgga tcgctcactg agcacacaag 300 gaaaattgcc atgaacaact cacgtatgtc ttccgtttca acacagaaaa caacaggacg 360 ttctgccctg ggaaccaaaa gcgcgctggc ggcgattatc gccaccacca tgatggtctc 420 agtcgcttcc gccgccagtc tgcaaaccac caaagcgaca gaagcggcgt caaccggctg 480 ggcaacgcag agcggcggca ccaccggcgg cgccaaagcg tcatcatcca aaatctacgc 540 ggtgaaaagc atcagcgaat tcaaagcagc actgaacgga accgattcgt cgcccaagat 600 catccaggtc accggagcaa ttgatatcag cggcggcaaa gcctacacca gctttgacga 660 tcagaaggcc cgcagccaaa tcagcattcc gtccaacacc accatcatcg gtattggcaa 720 caaaggcaaa ttcaccaacg gttcgctggt ggtgaaaggc gtcagcaacg ttatcctgcg 780 taacctgtac atcgaaacgc cggtggacgt ggcgccgcat tacgaagagg gagatggctg 840 gaacgccgag tgggacgcag tagtgattga cagcacagac cacgtctggg ttgaccatgt 900 caccatcagc gacggcagct tcaccgacga caaatacacc accaaaaacg gcgaaaaata 960 tgtgcagcat gacggctcgc tggatatcaa gcgcggctcc gactacgtta ccgtttctaa 1020 tagccgcttc gagctgcacg acaaaaccat cctgatcggc cacagcgaca acaacggctc 1080 gcaggacgcc ggcaaactgc gcgtcacctt ccacaacaac ctgtttgacc gggtcggcga 1140 acgtaccccg cgcgtacgct tcggcagcgt tcacgcttac aacaacgttt acgttggcga 1200 cgtcaaccac aaagcctatc gctatcagta cagcttcggt atcggcacca gcggtagcct 1260 gctgtctgag tccaacgcgt ttaccattga taacatgaaa aaaatcagcg gccgcgacaa 1320 agaatgcagc gtggtcaagg cgtttaacgg taagatcttc tctgataaag gctcgatcat 1380 caacggcgct tcgtacaatc tgaatggttg cggctttggc ttcagtgcat acagcgccaa 1440 gatcccgtac aaatactcag cccaaaccat caccaccagc ctggctggca gcatcagcag 1500 caacgcgggc tacggtaaac tgtaatttcc cgcccagcgt aaaggccact tgcaaaagtg 1560 gcctttattt tcccatattc ccaccactcg tattaccccg atgttctccg aagcgccact 1620 caatacggtt caggttaatc cggccttgcc acaggcgttc atcaagaaat cgaccggttt 1680 tccccatcaa atgcagggac tagcaggaat tagtaagagc cacagattaa taaaattcta 1740 tttaataaaa atcgaatcaa ttgttaactg tttgggttca tttcagataa aaacacttat 1800 acatatagct gaatttaaaa gaaaaattaa ttcaacattc ataaccaaaa gttaccggtc 1860 acgatcacac tttagataaa attaatcagc ctcataaaaa aaacgagatt ttgatcacaa 1920 aataaacaat cgaaaacgct taaaaatccg ccttgccaaa ggacaaaatg gcgtttcatt 1980 tttttcacaa acacttttca gtcaacaaaa ttggattagc gcagatagcg caaggaacca 2040 gtctatgaac aacacacgag tgtctttccg tagtaccaaa agcttgctgg cagccatcat 2100 cgctacatcg atgatgacct ggtcagtcaa tcgagccacg ctgcaaacca ccaaggcgac 2160 agaagcagcg tcaactggct gggcaacgca gggcggcacc accggcggcg ctaaagcagc 2220 ctcagccaaa atctatgcag taaaaaatat cagcgaattt aaagccgcgc tgaatggcac 2280 cgacaccgat ccgaaaatca ttcaggtgac cggcgctatc gatatcagcg gcggcaaagc 2340 ctacaccagc tttgacgatc agaaagcccg cagccagatc agcgttccgt ccaacaccac 2400 catcatcggt attggcagca atggcaaatt caccaacggc tctctggtca tcaaaggcgt 2460 gagtaacgtt atcctgcgta acctgtatat cgaaacgccg gtggatgtag caccgcatta 2520 cgaggaaggg gatggctgga acgccgagtg ggatgcggcg gtgatcgaca attccacccg 2580 cgtttgggtt gaccatgtca ccatcagcga cggcagcttc accgatgaca aatacaccac 2640 caaaaacggc gaaaaatatg tgcagcacga cggcgcgctg gatatcaaga aagggtccga 2700 ctacgtcacc atttccagca gccgcttcga actgcatgac aaaaccatcc tgatcggcca 2760 tagcgacagc aacggctcac aggactccgg caaactgcgc gttaccttcc acaacaacgt 2820 gtttgaccgt gtaaccgaac gtaccccgcg cgtacgcttt ggtagcatcc acgcgtataa 2880 caacgtgtat ctgggcgacg tgaaaaacag cgtctatccg tatctgtaca gcttcggtct 2940 tggcaccagc ggcacgatcc tgtcggagtc caactccttc acgctctcca acctgaagag 3000 cattgatggc aaaaatccag aatgcagcat cgtgaagcaa ttcaacagca aggtattctc 3060 cgataacggc tcactggtta acggctcgtc aaccactaag ctggacactt gcgccgtgac 3120 ggcttacaaa ccgactctgc cgtacaaata ttcggctcag accatgacaa gtagcctggc 3180 tagcagcatc aatagcaatg caggatacgg caaactgtaa ttcctctgcc agctaaatcg 3240 cggccacttg aaaaagtggc cttttttgca tcaatcgcag gtaaaagagg tcatcatcat 3300 gctcacaact acctggaatc gcgccttttt tctcggttcc ctgctttgtc tgccgatatc 3360 ttttgcgcag gctgaaggca ccgtgacgga aaccaacgct tcacctacgc cccacgagct 3420 caatgtcgtc acgctggctc ccaatacatc gatcagcggc agagtggcct accgtgacat 3480 acgcttccct gccacattac tcatcaaaga tcagcgtggc gtagaacgca acgtaaaaac 3540 cgatattcaa gggcgatttt at 3562 // |
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