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EBI DbfetchID X17022; SV 1; linear; genomic DNA; STD; PRO; 2284 BP. XX AC X17022; XX DT 12-DEC-1989 (Rel. 22, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 9) XX DE Citrobacter freundii (RFL9) cfr9IM gene for DNA DE (Cytosine-4)-Methyltransferase. XX KW cytosine methyltransferase. XX OS Citrobacter freundii OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Citrobacter. XX RN [1] RX DOI; 10.1093/nar/17.23.9823 RX PUBMED; 2690010. RA Klimasauskas S., Timinskas A., Menkevicius S., Butkiene D., Butkus V., RA Janulaitis A.; RT "Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: RT similarity to adenine and cytosine-C5 DNA-methylases"; RL Nucleic Acids Res. 17(23):9823-9832(1989). XX RN [2] RP 1-2284 RA Lubys A.; RT ; RL Submitted (29-MAR-1992) to the EMBL/GenBank/DDBJ databases. RL Institute of Biotechnology, Gene Engineering Department, Graiciuno 8, RL Vilnius, Lithuania, 2028 XX RN [3] RP 1-2284 RX DOI; 10.1016/0378-1119(94)90132-5 RX PUBMED; 8163180. RA Lubys A., Menkevicius S., Timinskas A., Butkus V., Janulaitis A.; RT "Cloning and analysis of translational control for genes encoding the Cfr9I RT restriction-modification system"; RL Gene 141(1):85-89(1994). XX FH Key Location/Qualifiers FH FT source 1..2284 FT /organism="Citrobacter freundii" FT /strain="RFL9" FT /mol_type="genomic DNA" FT /clone="clone pCfr9IRM2.3" FT /db_xref="taxon:546" FT CDS 281..1183 FT /transl_table=11 FT /gene="cfr9IM" FT /standard_name="Cfr9I methyltransferase" FT /product="DNA (Cytosine-4)-Methyltransferase" FT /function="Methyl transfer" FT /db_xref="GOA:P14243" FT /db_xref="HSSP:1G60" FT /db_xref="InterPro:IPR017985" FT /db_xref="UniProtKB/Swiss-Prot:P14243" FT /protein_id="CAA34887.1" FT /translation="MPSKKSSSPLSVEKLHRSEPLELNGATLFEGDALSVLRRLPSGSV FT RCIVTSPPYWGLRDYGIDEQIGLESSMTQFLNRLVTIFSEAKRVLTDDGTLWVNIGDGY FT TSGNRGYRAPDKKNPARAMAVRPDTPEGLKPKDLIGIPWRLAFALQEDGWYLRSDIVWN FT KPNAMPESVKDRPTRSHEFLFMLTKSEKYYYDWEAVREEKDSGGFRNRRTVWNVNTKPF FT AGAHFATFPTELIRPCILASTKPGDYVLDPFFGSGTVGVVCQQEDRQYVGIELNPEYVD FT IAVNRLQGEDTNVIRIAAA" FT CDS 1180..2172 FT /transl_table=11 FT /gene="cfr9IR" FT /standard_name="Cfr9I restriction endonuclease" FT /product="Restriction Endonuclease" FT /function="DNA cleavage" FT /db_xref="GOA:Q60132" FT /db_xref="InterPro:IPR019071" FT /db_xref="UniProtKB/Swiss-Prot:Q60132" FT /experiment="experimental evidence, no additional details FT recorded" FT /protein_id="CAA34888.1" FT /translation="MTNKIVFPEPKQQVDFAFSLKRFRGIYLQNALLETVRDMDIVALD FT TQLAEYVNKADLATLATYGLRAELLFPVPVLLETNPFLLGYYRLLMGYSQKEFYGKDKG FT FNAGCFKSMEVKGNIGKVAKPKISELCHAFCSVASSLLQGVGPLRISRELLDDLTLLTV FT GPQLRGGANNQRGADGIVLVFEIIKEIVSHAVAEVRENAIEVNSATGRNVLIEFAPDPD FT IIIREEMSLDNYRNVVAIEVKSGTDVSNIHNRIGEAEKSHQKARGHGYTECWTVVNVSR FT LDMDKARKESPSTNRFYSITDLSLREGEQYEDFRRRVLSLTAISAAPTP" XX SQ Sequence 2284 BP; 624 A; 438 C; 570 G; 652 T; 0 other; aagggaagcc taaccagacc gggaaagggt ctggctctgc cgtggtgttc accgagccat 60 aaacgatcgg tactcgataa gagagcggtt aactgacaat ctgatgatac gccccggcgc 120 ttttgttggt atgcccaaaa cggcgctaaa cggtcgctat tgtctgcttt tagttcggta 180 aggctgttgt tggagtatct gatgacgttt acactactgg cccagataga tttaaaagtt 240 gtaggttgtt gcatgtctgc attgtgcgtg aggaatattt atgccaagta aaaagagtag 300 ttcgccgctg agtgttgaga aacttcatcg ttctgagccc ttggagttga acggagctac 360 gctttttgaa ggtgatgctc tgtcagtatt gaggagactt ccgagcggct cagttcggtg 420 catcgtcact tctccgccat actgggggct acgtgattac ggcatagatg aacaaatcgg 480 tttagaaagt agcatgactc agtttttaaa tcgtcttgtt acgatctttt ctgaggcgaa 540 acgtgtattg actgacgacg gaacgctatg ggttaacatt ggtgatggat atacaagcgg 600 aaatcgcggg tatagagctc ctgataagaa aaatccggca cgagctatgg ctgttcgccc 660 ggatacgcca gaaggactaa aaccgaagga tctgattggg attccttggc ggttagcgtt 720 cgctttgcaa gaagatgggt ggtacctacg aagcgacatt gtttggaata aacctaacgc 780 gatgcctgaa agtgtaaaag accggcctac ccgttctcat gagttccttt ttatgctgac 840 caaatcagag aaatattatt acgattggga agcggtgaga gaagaaaaag atagcggagg 900 tttcagaaat cgacgcacag tatggaatgt aaatacgaaa ccttttgcag gggctcattt 960 cgcaacattc ccaacggagc taattcgtcc atgcatcttg gcatccacga aacctggtga 1020 ttacgtatta gatccattct tcggctctgg tactgtaggc gttgtatgcc agcaggaaga 1080 ccgccagtat gttggtattg aactcaatcc agaatatgtt gatatagctg taaatcgttt 1140 gcagggtgag gatacaaatg tgataaggat cgcggcagca tgactaataa aatagttttc 1200 cctgaaccaa agcagcaagt tgatttcgct ttttccttaa aacgatttcg tggtatctat 1260 ctccagaatg cactgcttga aacagttcgt gatatggata tcgtagcgct tgatacccag 1320 cttgccgagt atgttaataa agcagatctg gcaactctcg caacatacgg tttacgtgca 1380 gagcttctct tccctgtacc tgttttattg gagaccaatc cttttctttt aggctactat 1440 cgtcttttaa tgggatacag ccagaaagag ttttatggga aggataaagg cttcaatgct 1500 ggttgcttca aatccatgga agtgaaaggg aatattggca aagttgcaaa acctaaaata 1560 tctgaacttt gtcatgcttt ttgctcagtg gcatcttctc ttttgcaagg agttggaccg 1620 ctgaggatta gtcgggagct tcttgatgac ttgacacttc tcaccgttgg tccacaactt 1680 cgcgggggcg ccaataatca gcgtggagct gatggtatcg tattagtgtt tgaaataata 1740 aaagaaattg tttcgcatgc agtggctgag gttagggaga acgctattga ggttaatagt 1800 gcgacaggcc gaaatgttct aatcgagttt gctccagatc cagatatcat cattcgggaa 1860 gagatgtccc tcgataacta tcgaaatgtg gttgcaatcg aagttaaaag tggtactgat 1920 gtttcaaata ttcataatcg tattggtgaa gctgagaaaa gccatcaaaa agcacgaggc 1980 catggatata cggaatgttg gacggtggtt aatgtatccc gtttagatat ggacaaggct 2040 cggaaagaat ctccttctac gaatcgattt tattcaataa ctgatttgtc tcttagagaa 2100 ggagaacaat atgaagattt tagacggcga gttttatcct tgactgcgat ttctgcggct 2160 ccaacgccat aaaagtcata ttaactgtct tgttcagaat aaggtaaaag ctcgctgatt 2220 actctcttga acagcaagcc tctaatttta gctgattact ggttactgct ggtttatggg 2280 atcc 2284 // |
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