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EBI DbfetchID X16985; SV 1; linear; genomic DNA; STD; PRO; 1536 BP. XX AC X16985; XX DT 12-DEC-1989 (Rel. 22, Created) DT 21-JUN-1994 (Rel. 40, Last updated, Version 5) XX DE Micrococcus varians plasmid borne mvaIM gene for DNA-methylase (MvaI). XX KW DNA methylase; restriction modification enzyme. XX OS Micrococcus luteus OC Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales; OC Micrococcineae; Micrococcaceae; Micrococcus. OG Plasmid XX RN [1] RP 1-1536 RA Klimasauskas S.; RT ; RL Submitted (20-OCT-1989) to the EMBL/GenBank/DDBJ databases. RL Klimasauskas S., Institute of Applied Enzymology, Fermenty 8, Vilnius, RL 232028, Lithuanie, USSR. XX RN [2] RP 1-1536 RX DOI; 10.1093/nar/17.23.9823 RX PUBMED; 2690010. RA Klimasauskas S., Timinskas A., Menkevicius S., Butkiene D., Butkus V., RA Janulaitis A.; RT "Sequence motifs characteristic of DNA[cytosine-N4]methyltransferases: RT similarity to adenine and cytosine-C5 DNA-methylases"; RL Nucleic Acids Res. 17(23):9823-9832(1989). XX FH Key Location/Qualifiers FH FT source 1..1536 FT /organism="Micrococcus luteus" FT /plasmid FT /strain="RFL19" FT /mol_type="genomic DNA" FT /db_xref="taxon:1270" FT promoter 64..69 FT /note="-35 region" FT promoter 87..92 FT /note="-10 region" FT RBS 140..144 FT /note="pot.ribosome binding site" FT CDS 151..1515 FT /transl_table=11 FT /note="MvaI DNA methylase (AA 1-454)" FT /db_xref="GOA:P14244" FT /db_xref="InterPro:IPR017985" FT /db_xref="UniProtKB/Swiss-Prot:P14244" FT /protein_id="CAA34854.1" FT /translation="MEYLNDKDQHLIDKLSKKINDNNQYLGFLNTNTKELTHRYHIYPA FT MMIPQLAKEFIELTQQVKPEIKKLYDPFMGSGTSLVEGLAHGLEVYGTDINPLSQMMSK FT AKTTPIEPSKLSRAISDLEYSIREMTILYHEGNYKISNLPDFDRIDFWFKEEVIISLQL FT IKNCINEFIEDDLKTFFMAAFSETVRHVSNTRNNEFKLYRMAPEKLEIWNPNVTEEFLK FT RVYRNELGNMDFYRQLENVGNYSPKTIINKQSNIKLPEEFKDEMFDIVVTSPPYGDSKT FT TVAYGQFSRLSAQWLDLKIDDETKINQLDNVMLGGKTDKNIIVNDVLEYLNSPTSKSVF FT NLISHKDEKRALEVLQFYVDLDKSIKETTRVMKPESYQFWVVANRTVKMISIPTDIIIS FT ELFKKYNVHHLYSFYRKIPNKRMPSKNSPTNKIGNHSVTMTSEIILMLKNYINKS" XX SQ Sequence 1536 BP; 608 A; 199 C; 236 G; 493 T; 0 other; actaattcag caattaattg atcaaatttt tgacttctcg tcataactat tcctccttgt 60 tttttgatat gatagggcat atactatata ttactgaata ataaaaaggt ttttgtgtac 120 taatagtgca caaaaaccaa aggagacaaa atggaatatt taaatgataa agatcaacat 180 ttaattgata aattatcaaa aaagattaat gataataatc aatatcttgg tttcttaaat 240 acaaatacta aagaattaac tcatagatat catatttatc ctgctatgat gatccctcaa 300 ttggctaaag aattcattga attaactcaa caagtaaaac cagaaatcaa aaaattatat 360 gatcctttta tgggctctgg tacttcttta gtagaaggac ttgcacatgg gttggaagta 420 tatggaacag atataaatcc tctatcacaa atgatgagta aagctaaaac tactcctata 480 gaaccttcaa agttgtcgag agctatttca gatcttgaat attctataag agaaatgaca 540 attctgtatc atgaggggaa ttataaaata agcaaccttc ctgattttga tagaatagat 600 ttttggttta aagaagaagt tataataagt ctacagttaa taaaaaattg cataaatgag 660 tttatagaag atgatttgaa aacgttcttc atggcagcat ttagtgaaac agttaggcat 720 gtttcaaata ctcgtaataa tgaatttaaa ctgtatagaa tggcacctga aaaattagaa 780 atatggaatc cgaatgtaac tgaagaattt ttaaagagag tatacagaaa tgaattaggc 840 aatatggatt tctatagaca acttgaaaat gtaggaaatt actcgcctaa aactataata 900 aataagcaaa gcaacataaa acttccagag gaatttaaag atgaaatgtt cgatattgta 960 gttacttctc caccatatgg tgatagtaaa acaactgtag cctatgggca attttcaaga 1020 ttgtccgctc aatggttgga tctgaaaata gatgatgaga ctaaaataaa tcaattagat 1080 aatgtgatgc ttggtggaaa aacagataaa aatattattg ttaatgatgt gttagaatat 1140 ctcaattctc caacgtcgaa atcagtattt aatttaataa gtcataaaga tgaaaaaaga 1200 gcactagaag ttcttcaatt ttatgttgat ttggataaat ctattaaaga aactacaaga 1260 gtgatgaagc ccgagtcata tcaattttgg gtagtagcta atagaacagt aaaaatgatc 1320 agtataccaa ctgatattat aatttctgag ttatttaaaa agtataatgt tcatcattta 1380 tatagtttct ataggaaaat ccctaataaa cgtatgcctt caaaaaattc tcctactaat 1440 aaaataggta atcattctgt taccatgact tctgagatta tattaatgct aaaaaattac 1500 attaataaaa gctgatcttc ttcaatcatg cttaca 1536 // |
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