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EBI DbfetchID X16817; SV 1; linear; genomic DNA; STD; PRO; 3072 BP. XX AC X16817; XX DT 25-JAN-1990 (Rel. 22, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Klebsiella pneumoniae gyrA gene for DNA gyrase subunit A (EC 5.99.1.3) XX KW DNA topoisomerase; gyrA gene; gyrase A; topoisomerase. XX OS Klebsiella pneumoniae OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Klebsiella. XX RN [1] RP 1-3072 RA Das H.K.; RT ; RL Submitted (03-OCT-1989) to the EMBL/GenBank/DDBJ databases. RL Das H. K., Jawaharlal Nehru University, Genetic Engineering Unit, Centre RL for Biotechnology, New Dehli 110 067, India. XX RN [2] RP 1-3072 RX DOI; 10.1093/nar/18.1.151 RX PUBMED; 2155395. RA Dimri P.G., Das K.H.; RT "Cloning and sequence analysis of gyrA gene of Klebsiella pneumoniae"; RL Nucleic Acids Res. 18(1):151-156(1990). XX CC Author describes stem-loop structure in promoter region possibly CC acting as transcriptional control. CC CC Data kindly reviewed (02-MAR-1990) by Das H.K. XX FH Key Location/Qualifiers FH FT source 1..3072 FT /organism="Klebsiella pneumoniae" FT /strain="M5a1" FT /mol_type="genomic DNA" FT /clone="pGPD80" FT /db_xref="taxon:573" FT promoter 213..218 FT /note="pot.-35 region" FT misc_feature 216..233 FT /note="pot. stem-loop structure" FT promoter 238..243 FT /note="pot. -10 region" FT precursor_RNA 251..2969 FT /note="transcript" FT RBS 277..286 FT /note="pot. ribosome binding site" FT CDS 288..2918 FT /transl_table=11 FT /note="gyrase subunit A (AA 1-876)" FT /db_xref="GOA:P14829" FT /db_xref="HSSP:1AB4" FT /db_xref="InterPro:IPR013757" FT /db_xref="UniProtKB/Swiss-Prot:P14829" FT /protein_id="CAA34724.1" FT /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPVH FT RRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSLRYMLVD FT GQGNFGSVDGDSAAAMRYTEIRMSKIAHELMADLEKETVDFVDNYDGTEKIPDVMPTKI FT PNLLVNGSFGIAVGMATNIPPHNLTEVINGRLAYVEDEEISIEGLMEHIPGPDFPTAAI FT INGRRGIEEAYRTGRGKVYICARAEVEADAKTGRETIIVHEIPYQVNKARLIEKIAELV FT KEKRVEGISALRDESDKDGMRIVIEVKRDAVGRVVLNNLYSQTQLQVSFGINMVALHHG FT QPKIMNLKEIIAAFVRHRREVVTRRTILALRKARDRADILEALSIALANIDPIIELIRR FT APTPAEAKAGLIARSWDLGNVSAMLEAGDDAARPEWLEPEFGVRDGQYYLTEQQAQAIL FT DLRLQKLTGLEHEKLLDEYKELLEQIAELLHILGSADRLMEVIREELELVREQFGDARR FT TDITANSVDINIEDLITQEDVVVTLSHEGYVKYQPVNDYEAQRRGGKGKSAPRIKEEDF FT IDRLLVANTHDTILCFSSRGRLYWMKVYQVPEASRGARGRPIVNLLPLEANERYTAILP FT VREYEEGVNVFMATASGTVKKTPADEFSRPRSAGIIAVNLNEGDELIGVDLTSGQDEVM FT LFSAAGKVVRFKEDDVRAMGRTATGVRGIKLAGEDKVVSLIVPRGEGRILTATENGYRK FT RTAVAEYPTKSRATQGVISIKVTERNGSVVGAVQVDDCDQIMMITDAGTLVRIRVSEVS FT IVGRNTQGVILIRTAEDENVVALQRVAEPVDDEELDAIDGSAAEGDEDIAPEADTDDDI FT AEDEE" FT terminator 2941..2969 FT /note="pot. rho independent terminator" XX SQ Sequence 3072 BP; 712 A; 824 C; 889 G; 647 T; 0 other; gaattcgcct tctaaatccc accagcgcga ggcgacggct tcaaatttag cgatttcatt 60 atgatcaaca ttgtgggcca ccgacggttt ttcggcattc atgggcgctt ttactcctgt 120 tcagttcaga cccgcgagta tatcaggcct cagggatgaa taaaccgtct atccctgtcg 180 ccttccgccg cgcgaaaatc attgggtata ggtttacccg tatcactacg ggtgtgttat 240 aatttgcgac ctttgaatcc gggatacagt agagggatag cggttagatg agcgaccttg 300 cgagagaaat tacaccggtc aacattgagg aagagctgaa gagctcgtat ctggattacg 360 cgatgtcggt cattgttggc cgtgcgctgc cggatgtccg agatggcctg aagccggtac 420 accgtcgcgt actatacgcc atgaacgtat tgggcaatga ctggaacaaa gcctataaaa 480 aatctgcccg tgtcgttggt gacgtaatcg gtaaatacca ccctcatggt gatactgccg 540 tgtatgacac cattgtacgt atggcgcagc cattctccct gcgttacatg ctggtagatg 600 gccagggtaa cttcggttct gtcgacggcg actccgccgc agcgatgcgt tatacggaaa 660 tccgtatgtc gaagatcgcc catgaactga tggccgacct ggaaaaagag acggtcgatt 720 tcgtcgataa ctatgacggc acggaaaaga tccctgacgt tatgccgacc aaaatcccga 780 acctgttagt caacggttcg ttcggtatcg cggtaggtat ggcgaccaac attccgccgc 840 acaacctgac cgaagtgatc aacggtcgtc tggcctacgt tgaagacgaa gagatcagca 900 ttgaagggct gatggaacat attccgggcc cggacttccc gaccgccgcg atcatcaacg 960 gtcgccgcgg cattgaagag gcctatcgta ccggtcgcgg taaagtgtac atttgcgccc 1020 gcgcggaagt ggaagctgac gcgaaaaccg gtcgcgaaac catcatcgtg catgaaattc 1080 cgtatcaggt gaacaaagcg cgcctgattg agaaaatcgc tgagctggtc aaagaaaaac 1140 gcgtcgaagg catcagcgcg ctgcgtgacg agtctgataa agacggcatg cgtatagtga 1200 ttgaagtgaa gcgcgatgcg gtgggtaggg ttgtgctcaa caacctctac tcgcagactc 1260 agctgcaggt ctccttcggt atcaacatgg ttgccctgca ccatggtcag ccgaagatca 1320 tgaatctgaa agaaattatt gccgccttcg tgcgccaccg ccgcgaagtg gtgacccgcc 1380 gtacgatttt agcactgcgt aaagcccgtg atcgggcgga catccttgaa gcgctgtcga 1440 ttgccctggc caacatcgat ccgattattg agctgattcg ccgcgcgccg acgccggcgg 1500 aagcgaaagc tggcttaatc gcccgttcat gggatctggg caacgtttcc gcgatgctgg 1560 aagcgggcga tgacgccgcg cgtccggaat ggctggaacc tgaattcggc gtgcgcgacg 1620 gccagtacta cctgaccgaa cagcaggcac aggcgattct ggatctgcgt ttgcagaaac 1680 tgaccggcct tgagcatgaa aaactgctcg acgaatacaa agagctgctg gaacagattg 1740 cggaactgct gcatattctg ggcagcgccg atcgcctgat ggaagttatc cgcgaagagc 1800 tggagctggt ccgtgaacag ttcggcgacg cgcgccgtac cgacatcacc gctaacagcg 1860 tagacatcaa catcgaagac ctgatcaccc aggaagatgt tgtcgtgacc ctttcgcatg 1920 agggctacgt gaagtatcag ccggttaacg actacgaagc gcagcgtcgc ggcggtaaag 1980 gcaaatctgc gccgcgtatc aaagaagaag actttatcga ccgtctgctg gtggccaaca 2040 cccacgacac gatcctctgc ttctccagcc gcggtcgtct ctactggatg aaagtgtatc 2100 aggtgccgga agccagccgc ggcgcccgcg gtcgtccgat cgtcaacctg ctgccgctgg 2160 aagccaacga gcgctacacc gcgatcctgc cggtccgcga atacgaagag ggcgtgaacg 2220 tctttatggc caccgccagc ggtaccgtga agaaaacgcc agctgacgaa ttcagccgtc 2280 cgcgttccgc cggtattatc gcggttaacc ttaacgaagg cgacgaacta ataggcgtcg 2340 atctgacctc cggtcaggac gaagtcatgc tgttctccgc cgccggtaaa gtggtgcgct 2400 ttaaagagga cgacgtccgc gcgatgggcc gtaccgccac cggcgtgcgc ggcatcaagc 2460 tggccggcga ggacaaggtg gtctcgctga tcgttccgcg cggcgaaggc cgcatcctga 2520 ccgctacgga aaacggttac cgtaaacgta ccgcagtggc ggaatatcca accaagtcgc 2580 gtgcgaccca gggcgttatc tctatcaagg tcaccgaacg caacggttcc gttgttggcg 2640 cggtgcaggt agatgactgc gaccagatta tgatgatcac cgatgcgggc acgctggtac 2700 gaatccgcgt ttcggaagtg agcattgtcg gtcgtaacac tcagggcgtg attctgatcc 2760 gcaccgcgga agatgaaaat gtggttgctc tgcagcgcgt tgctgagccg gttgatgacg 2820 aagagctgga tgccatcgac ggcagcgcgg cggaaggcga tgaggatatc gcgccggaag 2880 cggataccga cgatgatatt gccgaagacg aagagtaatt ctctgcggta attgttcttg 2940 aaagggtcgg ggtctacccc gacccttttt gttttcccgt tgcgggaccg ttgttttatc 3000 gttaccttaa cgccattcaa ttgagcccca tgcggccaga gcacctgcca cattgaaaat 3060 atatgcttgt tt 3072 // |
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