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EBI DbfetchID X16458; SV 1; linear; genomic DNA; STD; PRO; 2280 BP. XX AC X16458; XX DT 23-NOV-1989 (Rel. 21, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 4) XX DE Serratia marcescens smaIR and smaIM genes for SmaI restriction endonuclease DE and SmaI modification enzyme XX KW modification enzyme; restriction endonuclease; smaIM gene; smaIR gene; KW unidentified reading frame. XX OS Serratia marcescens OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Serratia. XX RN [1] RP 1-2280 RA Heidmann S.; RT ; RL Submitted (01-SEP-1989) to the EMBL/GenBank/DDBJ databases. RL Heidmann S., Laboratorium fuer Molekulare Biologie-Genzentrum der LMU, RL Muenchen, Am Klopferspitz 18a, 8033 Martinsried, FRG. XX RN [2] RP 1-2280 RX DOI; 10.1093/nar/17.23.9783 RX PUBMED; 2690008. RA Heidmann S., Seifert W., Kessler C., Domdey H.; RT "Cloning, characterization and heterologous expression of the SmaI RT restriction-modification system"; RL Nucleic Acids Res. 17(23):9783-9796(1989). XX CC Data kindly reviewed (08-JAN-1990) by Heidmann S. XX FH Key Location/Qualifiers FH FT source 1..2280 FT /organism="Serratia marcescens" FT /strain="BMTU 1373" FT /mol_type="genomic DNA" FT /db_xref="taxon:615" FT promoter 82..87 FT /note="pot. TATA box for smaIR and small ORF" FT misc_feature 95..95 FT /note="transcription start site for smaIR and small ORF" FT RBS 106..112 FT /note="pot. ribosome binding site for small ORF" FT CDS 118..372 FT /transl_table=11 FT /note="small ORF (AA 1-84)" FT /db_xref="GOA:P14307" FT /db_xref="InterPro:IPR001387" FT /db_xref="UniProtKB/Swiss-Prot:P14307" FT /protein_id="CAA34477.1" FT /translation="MPDADICFMDIKEILAENVRSYRNINNLSQEQLAEISGLHRTYIG FT SVERKERNVTLSTLIILAKALNTSVPKLLTRQGLKNEQG" FT RBS 347..353 FT /note="pot. ribosome binding site for smaIR" FT CDS 359..1102 FT /transl_table=11 FT /note="smaIR (AA 1-247)" FT /db_xref="GOA:P14229" FT /db_xref="UniProtKB/Swiss-Prot:P14229" FT /protein_id="CAA34478.1" FT /translation="MSRDDQLFTLWGKLNDRQKDNFLKWMKAFDVEKTYQKTSGDIFND FT DFFDIFGDRLITHHFSSTQALTKTLFEHAFNDSLNESGVISSLAESRTNPGHDITIDSI FT KVALKTEAAKNISKSYIHVSKWMELGKGEWILELLLERFLEHLENYERIFTLRYFKISE FT YKFSYQLVEIPKSLLLEAKNAKLEIMSGSKQSPKPGYGYVLDENENKKFSLYFDGGAER FT KLQIKHLNLEHCIVHGVWDFILPPP" FT CDS complement(1088..1966) FT /transl_table=11 FT /note="smaIM (AA 1-292)" FT /db_xref="GOA:P14230" FT /db_xref="HSSP:1G60" FT /db_xref="InterPro:IPR017985" FT /db_xref="UniProtKB/Swiss-Prot:P14230" FT /protein_id="CAA34479.1" FT /translation="MKKHSNNLDLFDQTEECLESNLRSCKIIVGDAREAVQGLDSEIFD FT CVVTSPPYWGLRDYGNGGQIGAEDNINDYIKDLVDLFRDVRRTLKDDGTLWLNIGDSYT FT SGGRTWRDKDDKNKGRAMSYRPPTPEGLKPKDLIGVPWRLAFALQNDGWYLRTDIIWNK FT PNCQPESVRDRPTRSHEYIFLLSKGKKYYYDWESIKEPASDPKMDKKNRRTVWNINTEP FT YPGSHFAVFPRAMARLCVLAGSRPGGKVLDPFFGSGTTGVVCQELDRECVGIELNEEYA FT SLAKERILRRR" FT RBS complement(1971..1973) FT /note="pot. ribosome binding site for smaIM" FT misc_feature complement(1992..1992) FT /note="transcription start site for smaIM" FT promoter complement(2000..2005) FT /note="pot. TATA box for smaIM" XX SQ Sequence 2280 BP; 694 A; 462 C; 420 G; 704 T; 0 other; gttagagcga tacttagcgg tcccctcaga acatcccttc ttaaatgcta ctcatcgtct 60 gtcgacttat agtcatcacg gcattataaa ttccctttaa tttaaagggg atgacatgtg 120 cctgacgctg acatctgttt catggatatt aaagaaatcc ttgctgaaaa tgtaaggagc 180 tacaggaata tcaataattt atcgcaagaa cagttagcgg aaatatccgg tttgcacaga 240 acttacatag gttctgttga aagaaaagaa agaaatgtaa ctctaagtac tcttattatt 300 ttagctaaag cattgaatac ttccgtacca aaactcctaa caaggcaggg tttaaaaaat 360 gagcagggat gaccaactct ttacactttg gggaaagctt aacgatcgtc agaaggataa 420 ttttctaaaa tggatgaaag cttttgatgt agagaaaact taccaaaaaa caagtgggga 480 tattttcaat gatgattttt tcgatatatt tggtgataga ttaattactc atcatttcag 540 tagcacgcaa gctttaacaa aaactttatt cgaacatgct tttaatgact ccttaaatga 600 atctggagtt atatcctctc ttgcggaaag tagaacaaac cctgggcatg acataacaat 660 cgatagcata aaggttgctt taaaaacaga agcagctaaa aatattagca aatcatatat 720 tcatgtaagt aagtggatgg agttaggcaa gggggagtgg attctagaat tattattaga 780 acggttttta gagcatctag agaattatga acgtattttc acactcagat attttaaaat 840 atccgagtat aaatttagct accagcttgt agaaataccc aagagtcttt tgttggaagc 900 aaaaaatgcg aaattagaaa taatgtcggg aagcaaacaa agccctaagc ccggctatgg 960 atatgtgtta gatgaaaatg aaaataagaa gttttctcta tactttgatg gtggtgccga 1020 gagaaaactt caaataaaac atttaaattt agaacattgc attgttcatg gagtttggga 1080 ttttattcta ccgccgcctt aatattctct ctttggcaag agaagcatat tcttcattga 1140 gctcgatacc gacacactct ctatcgagtt cttggcaaac aacccctgtg gttcccgatc 1200 caaaaaacgg atcaagaact tttcctccgg gacgtgagcc agccaacaca cataacctag 1260 ccatggcacg tgggaaaaca gcgaagtgac ttcctggata cggttctgtg ttaatattcc 1320 aaacagttcg ccggttcttc ttatccattt taggatcaga tgccggttct ttaattgatt 1380 cccaatcata gtaatatttt ttccctttag ataagagaaa tatatattca tgtgatctag 1440 ttggtctatc tctgacgctt tcgggttgac agttcggttt gttccatatg atatcagtcc 1500 ttaaatacca accatcattt tgaagtgcga atgccagccg ccatggaaca ccgatcaaat 1560 cttttggttt taaaccttcc ggcgtaggcg gtctatagct cattgctcgc cctttgtttt 1620 tatcatcttt atcacgccaa gttcttcctc cagatgtata gctgtcacct atattaagcc 1680 atagcgttcc atcatcttta agagttcttc ttacatctct aaatagatca actaaatctt 1740 ttatgtaatc atttatgtta tcttcagcgc ctatttgacc gccattaccg taatctctta 1800 acccccaata aggtggggat gtaacaacac agtcaaatat ttcagaatct aacccctgaa 1860 ctgcttccct agcatcgcct acaataattt tgcaagaacg caggttgctc tccaaacact 1920 cttctgtttg atcgaataag tctaaattgt tactgtgttt tttcatagaa ctcaaagaaa 1980 caaagaaata atgctactta tggtattcat ttgagagcgt aaaaacaaca tttttagact 2040 caattccttt acgttgttca gttagctcaa cagccgcatt ttcgtccgtc gaagaagcta 2100 tagtgttgtt agcatctgct ttttgtagcc ggtggtctgc cagcactcag aatctccctg 2160 tatgcaacac ccgcctagct cacccggtag gtatctccac acttccggca gctactttta 2220 cgcagctcgg ccagctgttt atgcaacagc ttgttgtctt gtcgtatgag cccgatcagg 2280 // |
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