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EBI DbfetchID X16397; SV 1; linear; genomic DNA; STD; PRO; 2201 BP. XX AC X16397; XX DT 23-NOV-1989 (Rel. 21, Created) DT 22-MAR-1995 (Rel. 43, Last updated, Version 3) XX DE Erwinia carotovora pelB gene for pectate lyase (EC 4.2.2.2) XX KW pectate lyase; pelB gene. XX OS Pectobacterium carotovorum OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Pectobacterium. XX RN [1] RP 1-2201 RA Hinton J.C.D.; RT ; RL Submitted (29-AUG-1989) to the EMBL/GenBank/DDBJ databases. RL Hinton J.C.D., University of Warwick, Dept of Biological Sciences, Coventry RL CV4 7AL, UK. XX RN [2] RP 1-2201 RX DOI; 10.1111/j.1365-2958.1989.tb00164.x RX PUBMED; 2695748. RA Hinton J.C.D., Sidebotham J.M., Gill D.R., Salmond G.P.C.; RT "Extracellular and periplasmic isoenzymes of pectate lyase from Erwinia RT carotovora subspecies carotovora belong to different gene families"; RL Mol. Microbiol. 3(12):1785-1795(1989). XX FH Key Location/Qualifiers FH FT source 1..2201 FT /organism="Pectobacterium carotovorum" FT /strain="SCRI193" FT /mol_type="genomic DNA" FT /clone_lib="phC8" FT /clone="pB6" FT /db_xref="taxon:554" FT RBS 38..42 FT /note="ribosome binding site" FT CDS 49..1755 FT /transl_table=11 FT /note="precursor polypeptide (AA -19 to 548)" FT /db_xref="GOA:P14005" FT /db_xref="InterPro:IPR010702" FT /db_xref="UniProtKB/Swiss-Prot:P14005" FT /protein_id="CAA34432.1" FT /translation="MKRFALSLLAGLVALQASAATPDRLTIVNQYVDNVLTKAGDHYHG FT QSPTPLLADGVDPRTGKQMEWIFPDGRHAVLSNFSAQQNLMRVLVGLSNLSGNPSYKQR FT AEAIVKYHFQHYQDESGLLIWGGHRFVDLKTLQPEGPSEKEMVHELKNAYPYYDLMFSV FT DKDATARFIRGFWNAHVYDWKIMETSRHGKYGQKIGALWQSPFEQQPPFFATKGLSFLN FT AGNDLIYSASLLYKYNKEDGALVWAKRLAQQYVLPRDKATGLGVYQFTQALKRDETTDD FT ADTHSKYGDRAQRQFGPEFGPTALEGNMMLKGRTSTIYSENALMQLQLGKDLGAEGKEL FT LTWTTDGLKAFAKYAYNESDNTFRPMLANGKDLSNYVLPRDGYYGKKGTVIKPYPADNS FT FLLSYARAYTVLPDAELWRVARGIARAQGLGELGSAPGKDVKVDLATKNNDPYALFALL FT DLYQASKVKDYLSLAEKVGDNIISTRYQNGFFMAEPNRQYADVDTIEPYALLALEAAVR FT NQPQSVAPFLNGAGFTEGGYRMEDGSTRVSTRDNEIFLLNVGETLKPNNKK" FT sig_peptide 49..106 FT /note="signal peptide (AA -19 to -1)" FT mat_peptide 107..1752 FT /note="mature polypeptide (AA 1-548)" XX SQ Sequence 2201 BP; 556 A; 591 C; 561 G; 493 T; 0 other; gatcctttta ttacagctct ggattaatca tcgttacagg tagataaaat gaaaagattt 60 gcgctgtcgc tccttgcggg tctggtcgct ttacaggcca gcgccgctac gcctgaccgc 120 ctcaccatcg tcaatcagta tgttgataac gtgctgacaa aagccggtga ccactatcac 180 ggtcagtcgc ctacgccatt acttgctgat ggtgtcgatc ctcgcacggg caaacaaatg 240 gagtggatct tccccgatgg tcgccacgcc gtgttatcca acttttccgc acagcagaac 300 ctgatgcgcg tgctggtcgg gctcagcaac ctgagcggga accccagcta taagcaacgt 360 gccgaagcga ttgtgaaata ccattttcaa cattatcagg atgagagcgg cctgctgatt 420 tggggcggcc accgttttgt ggatctaaaa acgctgcaac cggaaggccc cagcgaaaaa 480 gagatggtgc atgagctgaa aaatgcctat ccctactacg atttgatgtt cagcgtcgat 540 aaagacgcaa ccgcacgctt tattcgcggt ttctggaatg cgcacgttta tgactggaag 600 attatggaaa ccagtcgtca cggtaaatac gggcaaaaaa taggtgcgct ctggcaaagt 660 ccgtttgagc aacagccgcc cttcttcgcc accaaaggcc tcagcttcct gaatgcgggg 720 aacgatctga tctattccgc atcactactg tacaaataca ataaagaaga cggcgcgctg 780 gtctgggcaa aacgtctggc acagcagtac gtgctgccac gtgataaagc gaccgggctc 840 ggcgtatatc agtttacgca ggcgctgaag cgcgatgaaa ctaccgacga tgccgatacg 900 cattcaaaat acggcgatcg cgcccagcgt cagtttggcc cggaattcgg ccccactgcg 960 ctggaaggca atatgatgct gaaaggacgc accagcacaa tctattccga aaatgcgctc 1020 atgcagctcc agttgggcaa agatttaggt gcggaaggca aagaacttct gacgtggaca 1080 accgatggcc tgaaagcctt tgccaaatat gcctataacg agtcagataa cacgttccgc 1140 ccgatgctgg ctaacggcaa agatctctcc aactacgttc tgccgcgtga cggttactac 1200 ggtaaaaaag gcaccgtgat caagccttat cctgcggata attcattcct gctttcctat 1260 gctcgcgcct ataccgtgtt accggacgcc gagctgtggc gtgtcgcacg cggcatcgct 1320 cgcgcacagg ggttaggtga gttaggttca gcaccgggta aagacgttaa agtggatctc 1380 gccaccaaga acaacgatcc ttacgccctg ttcgcgttgc tggatctgta tcaggctagc 1440 aaagtgaaag actatctgtc gctggcggaa aaagtgggcg ataacattat cagcacacgt 1500 tatcaaaacg gcttctttat ggccgagccc aacagacaat atgccgatgt cgataccatc 1560 gagccttatg ccctgttagc gttggaagct gcggtgcgca atcagccaca gtccgttgca 1620 ccgttcctga atggtgccgg cttcaccgaa ggtggctacc gtatggaaga tggctcaaca 1680 cgtgtgtcca ctcgcgataa cgagatcttc ctgttgaacg ttggcgaaac gttgaaaccc 1740 aacaacaaga aataagcgtt aatcctcaac acgccaatgg tggttcgctg ccattggcaa 1800 tcccctcttg cttactctac tcaaccctga accgaacaag accgacatta tcgggtgtga 1860 tacttaatcg tgtggcgctt aatcatgtgg ttttacagga tgcaatgccg gccacgacta 1920 acgtgaccag catgaagtaa cgcaataatt ttaagcgtta gttaaccaga ggcacctctg 1980 ggttcacacc aagccagccc ggcgtgggtt cgcctttcgc ttcaccgaca tacaggctca 2040 tctgtgagcg gaagcgctcg accgcaccgc gatcgtacat gaaatcagca aatgcctgcg 2100 gacgagtgtt acggtattcc cagatatgtt tgtaacctac tggcggctca acgtcggtgc 2160 gcaccgacca catacgcgag acctgagtct gcgtttggat c 2201 // |
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