EBI Dbfetch

ID   X15948; SV 1; linear; genomic DNA; STD; PRO; 1755 BP.
XX
AC   X15948; M36505;
XX
DT   21-JAN-1990 (Rel. 22, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 5)
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DE   Dictyoglomus thermophilum amyC gene
XX
KW   amyC gene; amylase.
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OS   Dictyoglomus thermophilum
OC   Bacteria; Dictyoglomi; Dictyoglomales; Dictyoglomaceae; Dictyoglomus.
XX
RN   [1]
RP   1-1755
RX   DOI; 10.1111/j.1432-1033.1988.tb14275.x
RX   PUBMED; 2458257.
RA   Horinouchi S., Fukusumi S., Ohshima T., Beppu T.;
RT   "Cloning and expression in Escherichia coli of two additional amylase genes
RT   of a strictly anaerobic thermophile, Dictyoglomus thermophilum, and their
RT   nucleotide sequences with extremely low guanine-plus-cytosine contents";
RL   Eur. J. Biochem. 176(2):243-253(1988).
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1755
FT                   /organism="Dictyoglomus thermophilum"
FT                   /strain="H-6-12"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="pYEJ001"
FT                   /clone="pDA401"
FT                   /db_xref="taxon:14"
FT   RBS             41..46
FT                   /note="pot. ribosome binding site"
FT   CDS             52..1548
FT                   /transl_table=11
FT                   /note="amylase (AA 1 - 498)"
FT                   /db_xref="GOA:P14899"
FT                   /db_xref="InterPro:IPR006046"
FT                   /db_xref="InterPro:IPR006589"
FT                   /db_xref="InterPro:IPR017853"
FT                   /db_xref="UniProtKB/Swiss-Prot:P14899"
FT                   /protein_id="CAA34072.1"
FT                   /translation="MKIKFFIKRTLIFIFILVTFLTYIHGYNEPWYKNAIFYEVFVRSF
FT                   ADSDGDRVGDLNGLIDKLDYFKNLNITALWLMPIFPSVSYHGYDVTDYYDIHPGYGTME
FT                   DFENLIRKAHEKNIKIILDLVVNHTSSRHPWFVSSASSYNSPYRDYYIWSTEKPEKNSN
FT                   LWYKKPTGYYYALFWSEMPDLNFDNPKVREEVKKIAKFWIEKGVDGFRLDAAKHIYDDD
FT                   SKNIQWWKEFYSYLKSIKPDVYLVGEVWDNEYKIAEYYKGLPSNFNFPLSDKIMNSSSK
FT                   SKRLRNYRISRLKRLFGENNTDFADAIFLRNHDQVRVRTFFGGSIDKSILAGSIYLTLA
FT                   GNTFIYYGEEIGMEGSKPDEYIREPFKWTDDMKSKYQTYWIIPRYNLPGNGIALDTEEK
FT                   DPNSIYNHYKKLLEIRVKCRALSNGKIERIKTQDRSILAYKLELEDEKIMVVHNLNRIE
FT                   NTFNFNNEIKEKDILYIRNAKTEKNKIILGPYSTVIVKIP"
FT   misc_feature    793..795
FT                   /note="active site Glu residue"
FT   misc_feature    991..993
FT                   /note="active site Asp residue"
XX
SQ   Sequence 1755 BP; 662 A; 286 C; 289 G; 518 T; 0 other;
     cccgggcttc taaaatttaa atttattaac acatgttaac ggaggtgaaa aatgaagata        60
     aagtttttta tcaaaagaac tcttattttc atattcatct tagttacttt tctaacttat       120
     attcatggat ataatgagcc atggtataaa aacgcgattt tttatgaagt ctttgtaaga       180
     tcttttgcag atagcgacgg ggatagagta ggagatttaa atggacttat agacaaatta       240
     gattacttta aaaatctaaa tattactgct ctatggctaa tgcctatctt cccctcagtg       300
     agttatcatg gatatgatgt cacagactat tatgatatcc atccaggtta tggtaccatg       360
     gaggactttg aaaacctaat aagaaaagcc catgaaaaaa atattaaaat aattcttgat       420
     ttggtagtaa accacacctc ttcaaggcat ccatggtttg tttcttctgc ctcttcttac       480
     aattctccgt atcgcgatta ttatatatgg agtactgaaa aacctgaaaa aaattctaat       540
     ttatggtata aaaaacctac aggatactac tatgcattat tctggtcaga aatgcccgat       600
     ctaaactttg ataatccaaa agtaagagaa gaggtcaaaa aaattgcaaa attttggata       660
     gaaaaaggtg ttgacggatt tagactcgac gcagcaaagc atatttatga tgatgactct       720
     aaaaacattc agtggtggaa agaattttac tcatatttaa agagtataaa acccgatgta       780
     taccttgttg gtgaagtttg ggataacgag tataagatag cagaatacta taaaggatta       840
     ccctcaaact ttaattttcc tctttctgac aaaataatga actccagtag caaatcaaaa       900
     agacttagga attatagaat ttctcgattg aagagacttt ttggggaaaa caatacagac       960
     tttgctgatg ccatattttt aagaaaccac gatcaagtaa gagttaggac attctttggt      1020
     ggaagcatag ataaaagtat tttggcagga tctatttact taactttggc cgggaatacc      1080
     ttcatatact acggagaaga aataggaatg gaaggatcaa aacctgacga atatataaga      1140
     gaaccgttta agtggactga cgatatgaaa tctaaatacc aaacctattg gattattcca      1200
     cgatataact taccaggaaa tggtatagcc ctcgataccg aagaaaaaga tccaaatagc      1260
     atatacaatc actataaaaa actcttagaa ataagagtca aatgcagagc tttatctaat      1320
     ggaaaaatag agagaataaa gactcaagat agatctatac ttgcctataa gttagaatta      1380
     gaggatgaaa aaataatggt agtccacaat ctaaatagga ttgaaaatac ctttaacttt      1440
     aacaacgaga taaaagaaaa agacatacta tatattagaa acgctaaaac tgaaaaaaat      1500
     aaaataatct taggacctta tagcactgtt atagtcaaaa tcccataaat aactccacat      1560
     atcttctgtc aaaatatagc cacctttttc ataagcaatc aatgctgctc caaccgcagg      1620
     tggaattttt aaaggaacaa ccctgcatct ttctgcataa actctcaatt tagcctgaat      1680
     atactctgga agccatgctg cacccttata aatactccct gcaataacta caggagtttc      1740
     ttcgtttaaa gatct                                                       1755
//