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EBI DbfetchID X15758; SV 2; linear; genomic DNA; STD; PRO; 3178 BP. XX AC X15758; M29331; XX DT 30-NOV-1989 (Rel. 22, Created) DT 16-SEP-2009 (Rel. 102, Last updated, Version 12) XX DE Lysinibacillus sphaericus restriction modification methylase gene, clone DE pES1 XX KW . XX OS Lysinibacillus sphaericus OC Bacteria; Firmicutes; Bacillales; Planococcaceae; Lysinibacillus. XX RN [1] RP 1-3178 RX DOI; 10.1016/S0022-2836(83)80123-5 RX PUBMED; 6313947. RA Posfai G., Kiss A., Erdei S., Posfai J., Venetianer P.; RT "Structure of the Bacillus sphaericus R modification methylase gene"; RL J. Mol. Biol. 170(3):597-610(1983). XX RN [2] RP 1-3178 RX DOI; 10.1093/nar/25.14.2841 RX PUBMED; 9207033. RA Finta C., Kiss A.; RT "Footprint analysis of the bsp RI DNA methyltransferase-DNA interaction"; RL Nucleic Acids Res. 25(14):2841-2846(1997). XX RN [3] RP 1-3178 RA Rasko T., Der A., Klement E., Posfai E., Medzihradszky K.F., Marshak D.R., RA Roberts R.J., Kiss A.; RT ; RL Unpublished. XX FH Key Location/Qualifiers FH FT source 1..3178 FT /organism="Lysinibacillus sphaericus" FT /mol_type="genomic DNA" FT /clone="pES1" FT /db_xref="taxon:1421" FT CDS 168..1082 FT /transl_table=11 FT /product="BspRI restriction endonuclease" FT /protein_id="CBE66553.1" FT /translation="MAQRKYGALEQKVANIFINEQVFTFKGKRYRVLKIGKPQKQGGSG FT EPKTDVYILGQELGGTDTVELKISVKKRDFEFIENKITPIRMADILGANWQKIIQESSE FT RIKDRFADCSLIYPERRGTTLKNSITLGWKAEITNRPRTLSARMQLSGQQIKDLLYKGV FT NLPPEKRDAIVDGNIIANSGIAEYILETDLDDIHTTADILSQMELIDDANLNEAYLAFT FT ANNYRTDTDKCDGARSLAVRVKWEIINGKLAHHIMFGQPLLYTGQAAVPELKQTLIQLG FT TIHPENMNDNSILDPSIVCKKKR" FT promoter 1096..1101 FT /note="-35 region" FT promoter 1119..1124 FT /note="-10 region rbs" FT precursor_RNA 1131..>1131 FT /note="primary transcript" FT CDS 1160..2434 FT /transl_table=11 FT /product="DNA(cytosine-5)methyltransferase" FT /db_xref="GOA:P13906" FT /db_xref="HSSP:1DCT" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/Swiss-Prot:P13906" FT /protein_id="CAA33764.2" FT /translation="MAIKINEKGRGKFKPAPTYEKEEVRQLLMEKINEEMEAVATATSD FT ISNDEIQYKSDKFNVLSLFCGAGGLDLGFELAGLEQSLGTDKALEAFKDRDVYNAIRHE FT SVFHTVYANDIFSEALQTYEKNMPNHVFIHEKDIRKIKEFPSANLVIGGFPCPGFSEAG FT PRLVDDERNFLYIHFIRCLMQVQPEIFVAENVKGMMTLGGGEVFRQIVEDFGAAGYRVE FT ARLLNARDYGVPQIRERVIIVGVRNDIDFNYEYPEITHGNEEGLKPYVTLEEAIGDLSL FT DPGPYFTGSYSTIFMSRNRKKKWTDQSFTIQASGRQAPIHPGGLPMEKVDKNKWIFPDG FT EENHRRLSVKEIKRIQTFPDWYEFSDGGNMKVSVNNRLDKQYKQIGNAVPVFLARAVAK FT SIAQFAADYLKDNHPHEAPQMKLFI" FT RBS 1147..1152 FT /note="pot.ribosome binding site" FT CDS 1244..2434 FT /transl_table=11 FT /product="DNA(cytosine-5)methyltransferase" FT /note="alternative translation methylase" FT /db_xref="GOA:Q59245" FT /db_xref="HSSP:1DCT" FT /db_xref="InterPro:IPR018117" FT /db_xref="UniProtKB/TrEMBL:Q59245" FT /protein_id="CAA33765.2" FT /translation="MEKINEEMEAVATATSDISNDEIQYKSDKFNVLSLFCGAGGLDLG FT FELAGLEQSLGTDKALEAFKDRDVYNAIRHESVFHTVYANDIFSEALQTYEKNMPNHVF FT IHEKDIRKIKEFPSANLVIGGFPCPGFSEAGPRLVDDERNFLYIHFIRCLMQVQPEIFV FT AENVKGMMTLGGGEVFRQIVEDFGAAGYRVEARLLNARDYGVPQIRERVIIVGVRNDID FT FNYEYPEITHGNEEGLKPYVTLEEAIGDLSLDPGPYFTGSYSTIFMSRNRKKKWTDQSF FT TIQASGRQAPIHPGGLPMEKVDKNKWIFPDGEENHRRLSVKEIKRIQTFPDWYEFSDGG FT NMKVSVNNRLDKQYKQIGNAVPVFLARAVAKSIAQFAADYLKDNHPHEAPQMKLFI" FT repeat_region 2443..2455 FT /rpt_type=INVERTED FT /note="inverted repeat A" FT repeat_region 2463..2475 FT /rpt_type=INVERTED FT /note="inverted repeat A'" XX SQ Sequence 3178 BP; 1135 A; 486 C; 677 G; 880 T; 0 other; tttcggaaat cgggctaaga attaaaaagt aaagcgagtc aactaggatt tgaaatcgaa 60 gagccataaa agttcttttt tattaaaaat attttaataa catagaagga atattatact 120 agtatcgcga atcttataat ctatcaaaaa tttggtaggt gaaagatttg gcgcagagaa 180 aatatggtgc attagagcaa aaggtagcaa acatatttat taacgaacaa gtattcacgt 240 ttaaaggtaa acgatatcgc gttttaaaaa ttggaaaacc gcaaaaacaa gggggttcag 300 gtgaacctaa aacggatgta tatattcttg gacaagaatt gggtggtacg gatactgttg 360 aattgaaaat ttcagtaaaa aagagagact ttgaattcat agaaaataaa attacaccaa 420 tacgtatggc agacatatta ggggcgaatt ggcagaaaat tatccaagaa tcctcagaac 480 gtatcaaaga tcgcttcgca gattgttctt taatttatcc agaacgaaga gggaccacat 540 tgaagaattc cattacgctt gggtggaaag cagaaattac aaatcgacct agaacattat 600 cagcaaggat gcaattatct gggcaacaaa taaaagattt gttatataaa ggggtaaacc 660 tacctcctga aaaaagggat gccattgttg atgggaatat cattgccaat tcaggtatcg 720 ctgagtacat attagagaca gacttggatg atattcatac gacggcagat attttatcac 780 aaatggaatt aatcgatgat gcaaacttaa atgaagcata tttggctttt actgcaaata 840 attatcggac agatacagat aagtgtgacg gtgcaagatc gctggctgtt cgagtcaaat 900 gggaaattat aaatggcaaa cttgctcatc atattatgtt tggtcagcca ttgctctaca 960 cgggacaagc agcagtgcct gagttgaagc aaactttaat acagttagga acgattcatc 1020 ctgaaaatat gaacgataat agtatcttag acccatcaat tgtttgtaaa aaaaaacgtt 1080 agcaaacttt acatattgtc acgacgatga agttccgtta aacttaaggg atgagaattt 1140 tgtcgaaagg ggcaacaaca tggctattaa gataaatgaa aaaggcagag gtaagttcaa 1200 gccagctcct acctatgaaa aagaagaagt aagacaacta ttgatggaaa aaatcaatga 1260 ggaaatggag gctgttgcta ctgcaacaag tgatatttcc aatgatgaaa ttcaatataa 1320 atcggataag tttaatgttc tttctttgtt ctgtggagca ggaggattag acctaggttt 1380 tgaacttgct ggtctagaac aaagccttgg aacggataaa gcactagaag cgtttaaaga 1440 tcgagacgta tataatgcaa ttcggcatga aagtgttttc catactgtct atgcgaatga 1500 tatttttagt gaagccttac aaacctatga aaagaatatg cccaaccatg tgtttattca 1560 tgaaaaggat atacgaaaaa taaaagaatt tccatctgcg aatcttgtta tcggtggatt 1620 tccttgtcca gggttttctg aggcaggacc aagacttgtg gatgatgaga gaaacttttt 1680 atacatacac ttcatccgtt gcctcatgca agtacaacct gaaattttcg tcgccgaaaa 1740 tgtaaaaggg atgatgaccc ttggaggcgg agaggtattt agacaaattg tagaggattt 1800 cggtgcagcg ggttaccgtg tggaagcaag gcttttaaac gcaagagact acggcgttcc 1860 gcaaattaga gaacgtgtga taatcgttgg ggttagaaac gatatagatt tcaactatga 1920 atacccagaa attacacatg gaaacgaaga aggattaaaa ccatatgtga cgctagaaga 1980 ggcgattggt gacttgagtt tagacccagg accttacttt acagggagtt attcgacgat 2040 ctttatgagt cgtaatcgga aaaagaaatg gactgaccag tcctttacga tacaagcaag 2100 tggtcgacag gcaccgattc acccaggtgg tcttccaatg gagaaagtgg ataaaaacaa 2160 atggattttc cctgatggcg aagagaacca tcgtagactt tctgttaaag aaattaagcg 2220 aatacaaaca tttcctgatt ggtatgaatt tagtgatggt ggcaacatga aagtgtctgt 2280 taataatcga ttggacaagc agtataaaca aattggaaat gcagtacctg tctttttggc 2340 tcgtgcggtt gctaagtcca ttgctcagtt tgcagcagat tatttgaaag ataatcatcc 2400 tcatgaagca ccgcaaatga aattatttat ttaggtatga ctaaaaatac catccatcaa 2460 taggatagta tttttttagt aggaaaatga tctcgcaatc acgaattaaa atatcttaga 2520 caggagaggt aaaacatgaa tgaacagcaa acattggatt taacgaacat tcatataaca 2580 aatgtaaagg ggaaaccttt tcgaaatgat ttatatgatg tggaatttga actaaatggg 2640 gaacctttcg ttggacgact acaaaaaagg aaaagtggca cattcattct tctttcaatc 2700 gaaagtgaag aaagaacggt tatgattgtc catggaaaca cgccagagga aacgcaacaa 2760 attgagaaga ttgagccaca tcttgtcgaa caagtaatag agcaaatata atgaactcca 2820 tcctttttgg atggtgtttt tttgtgcaca ccatgatgaa ccaattttat agattacctt 2880 tacgtatagt ttttaacaaa attgggcata acttacctat gagaagaaaa cgcttcaagc 2940 ctagactatc aactactatg ctactgttct attggatgat gattgaaaag gggaaaggca 3000 aagggacggt tattatggtt cacaaatatg cagtaaagtt gaatatccac caacaacaat 3060 acctagtgaa atgtaatgtg atggatgaag aattctatgt atttttagaa gaggaattga 3120 cacatgtttg ccattctttg aacgatttag attcatggat taattcgaaa atgatatc 3178 // |
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