spacer
spacer
EBIDatabasesDatabase BrowsingDbfetch

EBI Dbfetch

ID   X15708; SV 1; linear; genomic DNA; STD; PRO; 1260 BP.
XX
AC   X15708;
XX
DT   17-DEC-1989 (Rel. 22, Created)
DT   18-APR-2005 (Rel. 83, Last updated, Version 7)
XX
DE   Streptomyces cacaoi beta-lactamase gene (EC 3.5.2.6)
XX
KW   antibiotic resistance; beta-lactamase.
XX
OS   Streptomyces cacaoi
OC   Bacteria; Actinobacteria; Actinobacteridae; Actinomycetales;
OC   Streptomycineae; Streptomycetaceae; Streptomyces.
XX
RN   [1]
RP   1-1260
RA   Forsman M.;
RT   ;
RL   Submitted (30-JUN-1989) to the EMBL/GenBank/DDBJ databases.
RL   Forsman M., Department of Cell and Microbiology, Swedish Defence Research
RL   Establishment, S-90182, Umea, Sweden.
XX
RN   [2]
RP   1-1260
RX   DOI; 10.1111/j.1365-2958.1989.tb00125.x
RX   PUBMED; 2559297.
RA   Forsman M., Lindgren L., Haeggstroem B., Jaurin B.;
RT   "Transcriptional induction of Streptomyces cacaoi beta-lactamase by a
RT   beta-lactam compound";
RL   Mol. Microbiol. 3(10):1425-1432(1989).
XX
DR   CABRI; DSM 40057.
XX
FH   Key             Location/Qualifiers
FH
FT   source          1..1260
FT                   /organism="Streptomyces cacaoi"
FT                   /strain="DSM 40057"
FT                   /mol_type="genomic DNA"
FT                   /clone_lib="pACYC184"
FT                   /db_xref="taxon:1898"
FT   repeat_region   29..88
FT                   /note="sequence containing 6 10 bp direct repeats"
FT   promoter        132..137
FT                   /note="-35 region"
FT   promoter        156..161
FT                   /note="-10 region"
FT   misc_feature    168..168
FT                   /note="transcription start site"
FT   RBS             259..264
FT                   /note="pot. ribosome binding site"
FT   CDS             272..1216
FT                   /transl_table=11
FT                   /product="beta-lactamase preprotein (AA -26 to 288)"
FT                   /db_xref="GOA:P14560"
FT                   /db_xref="HSSP:1I2S"
FT                   /db_xref="InterPro:IPR000871"
FT                   /db_xref="UniProtKB/Swiss-Prot:P14560"
FT                   /protein_id="CAA33739.1"
FT                   /translation="MLHTRIRRATLGAVAALSLVPVMACGQESSADAAEPAGSAPSSSA
FT                   AAHKPGEVEPYAAELKALEDEFDVRLGVYAVDTGSGREVAYRDGERFPYNSTFKALECG
FT                   AVLDKHTDREMDRVVKYSEDDLVDNSPVTEKHVEDGMTLTALCDAAVRYSDNTAANLLF
FT                   ETVGGPKGLDKTLEGLGDHVTRMERVEPFLSRWEPGSKRDTSTPRAFAKDLRAYVLGDV
FT                   LAEGDRKQLTTWLRNNTTGDGLIRAGVRQGWVVGDKTGTGSYYGARNDMAVVWRPDGRP
FT                   LVLNVMVHGHTKDAELDSELIARATEVVADRLG"
FT   sig_peptide     272..349
FT                   /note="signal peptide (AA -26 to -1)"
FT   mat_peptide     350..1213
FT                   /note="beta-lactamase (AA 1 - 288)"
FT   misc_feature    546..579
FT                   /note="active site (AA 92 - 99)"
XX
SQ   Sequence 1260 BP; 187 A; 466 C; 422 G; 185 T; 0 other;
     atctcccgta ctctccccgc ttcttcggcg gctcttcctg cctcgtcctg cctcgtcctg        60
     cctcttcctg cctcttcctg cctcgtcacg gctcttcatg ccgttccggc atggaaccgc       120
     gcatacctgt attggaccgc atggccggca gcggcgaagg tgagtaccgg cacccgggcc       180
     cgccgacggg acggcggggt gccgctcccg tctttctgcg ctccatccct ttcagccgcc       240
     ccgatcgcgg ggcgacccgg aggttcggaa catgctgcac acacgtattc ggcgtgccac       300
     cctcggcgcc gtggccgcgc tgtccctcgt accggtgatg gcctgcggcc aggagagctc       360
     cgctgacgcc gccgagcccg cgggctccgc gccctcctcg tccgccgcgg cacacaagcc       420
     cggcgaggtc gagccgtacg ccgccgaact caaggcgttg gaggacgagt tcgacgtgcg       480
     gctgggcgtc tacgccgtcg acaccggctc cggccgcgag gtggcctacc gcgacgggga       540
     acgcttcccc tacaactcca ccttcaaggc gctggaatgc ggcgccgtgc tcgacaagca       600
     cacggaccgc gagatggacc gggtggtgaa gtactccgag gacgacctgg tcgacaactc       660
     gcccgtgacc gagaagcacg tcgaggacgg gatgacgctc accgcgctgt gcgacgcggc       720
     cgtccgctac agcgacaaca ccgccgccaa cctgctgttc gagacggtcg gcgggcccaa       780
     gggcctcgac aagacgctcg aagggctggg cgaccatgtc acgcggatgg aacgcgtcga       840
     gccgttcctc agccgctggg agcccggcag caagcgggac acctcgacgc cccgcgcgtt       900
     cgccaaggac ctgcgggcct atgtcctcgg tgacgtgctg gccgagggcg accggaagca       960
     gctgaccacg tggctgcgca acaacaccac gggggacggg ctgatccggg cgggcgtgcg      1020
     ccagggctgg gtcgtcggcg acaagaccgg cacgggcagc tactacggcg cgcgcaacga      1080
     catggccgtc gtctggcggc cggacggccg tccgctcgtc ctcaacgtca tggtgcacgg      1140
     ccacacgaag gacgccgagc tggacagcga gctcatcgcc cgggccacgg aggtcgtcgc      1200
     cgaccggctc ggctgatccc cgccctcgac ggctcgtccg gtggtccgcc ggacgtgtcc      1260
//
spacer
spacer