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EBI DbfetchID X15425; SV 1; linear; genomic DNA; STD; PLN; 5119 BP. XX AC X15425; XX DT 15-JAN-1990 (Rel. 22, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 3) XX DE Cucumber DNA for malate synthase (EC 4.1.3.2) XX KW malate synthase. XX OS Cucumis sativus (cucumber) OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; OC Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; OC eurosids I; Cucurbitales; Cucurbitaceae; Cucumis. XX RN [1] RP 1-5119 RA Graham I.A.; RT ; RL Submitted (06-JUN-1989) to the EMBL/GenBank/DDBJ databases. RL Graham I.A., Edinburgh University, Dept of Botany, The Kings Bldgs, RL Mayfield Road, Edinburgh, EH9 3JH, Scotland. XX RN [2] RP 1-5119 RX DOI; 10.1007/BF00016022 RX PUBMED; 2491683. RA Graham I.A., Smith L.M., Brown J.W.S., Leaver C.J., Smith S.M.; RT "The malate synthase gene of cucumber"; RL Plant Mol. Biol. 13(6):673-684(1989). XX FH Key Location/Qualifiers FH FT source 1..5119 FT /organism="Cucumis sativus" FT /strain="Long green ridge" FT /mol_type="genomic DNA" FT /dev_stage="mature leaves" FT /clone_lib="lambda gt11" FT /clone="lambda MS11" FT /db_xref="taxon:3659" FT misc_feature 1724..1729 FT /note="pot. CRE consensus (cAMP responsive element)" FT TATA_signal 1823..1830 FT precursor_RNA 1857..4676 FT /note="transcript" FT precursor_RNA 1857..4629 FT /note="transcript" FT exon 1857..2228 FT /number=1 FT CDS join(1896..2288,2712..3037,3421..3751,3825..4481) FT /product="malate synthase" FT /db_xref="GOA:P08216" FT /db_xref="InterPro:IPR019830" FT /db_xref="UniProtKB/Swiss-Prot:P08216" FT /protein_id="CAA33465.1" FT /translation="MGSLGMYSESGLTKKGSSRGYDVPEGVDIRGRYDEEFAKILNKEA FT LLFIADLQRTFRNHIKYSMECRREAKRRYNEGGLPGFDPATKYIRDSEWTCAPVPPAVA FT DRRVEITGPVERKMIINALNSGAKVFMADFEDALSPNWENLMRGQINLKDAVDGTISFH FT DRVRNRVYKLNDRTAKLFVRPRGWHLPEAHIFIDGEPATGCLVDFGLYFFHNHANFRRS FT QGQGYGPFFYLPKMEHSREAKIWNSVFERAEKMAGIERGSIRATVLIETLPAVFQMNEI FT LYELRDHSVGLNCGRWDYIFSYVKTFQAHPDRLLPDRVLVGMTQHFMRSYSDLLIRTCH FT RRGVHAMGGMAAQIPIRDDPKANEVALELVRKDKLREVKAGHDGTWAAHPGLIPACMEV FT FTNNMGNAPNQIRSMRRDDAANLTEEDLLQQPRGVRTMEGLRLNTRVGIQYLAAWLTGA FT GSVPLYNLAEDAATAEISRVQNWQWLKYGVELDGDGLGVRVNKELFGRVVEEEMERIER FT EVGKERFKKGMYKEACKMFTRQCTAPNLDDFLTLDAYNYIVIHHPRELSKL" FT intron 2289..2711 FT /number=1 FT exon 2712..3037 FT /number=2 FT intron 3038..3420 FT /number=2 FT exon 3421..3751 FT /number=3 FT intron 3752..3824 FT /number=3 FT exon 3825..4629 FT /number=4 FT polyA_site 4629..4629 FT polyA_site 4676..4676 XX SQ Sequence 5119 BP; 1587 A; 959 C; 947 G; 1626 T; 0 other; cgatacagta ctcttatctc tacccaagtc aaatcaccaa tatcactaat gtcccattaa 60 tatcactaat atataaatat cactgatata attgatatca atcaaagtta agtcactgat 120 attacaaata ttattcaact tttctctagc atcagtaacc taatcgttta gggcttcatg 180 tatgccttta tatattgaag ttgttgataa gatatgaatt aaaacttgct tctgagactc 240 accacaaaaa ggtatcaagt tgaatactga tttgaaaaga tacttgaagc tcctttgggc 300 ctacactcaa aacagctttc acacctcata attgattgac agaaagaagt taccaagaaa 360 taccgttgaa taatttctct tgtaaaagaa agaaatatat agaaaaagag gaaaaagaaa 420 aagagtcatc acaacccgta attagtatct tttttgaagt gttgtactct tgacattccc 480 tgaaataaaa tttctgatgg acgatcttgc taagtggatg gaggcttctt tagccgaatc 540 actctaaatc ttcttcttct caatctcttc ttctgtggtg ttcagtttct tggctgcatt 600 atttcagaat caaatgaagt gttttccttc ttcttttgtt tttgtccata tcttcataat 660 ctttaatttc aagtgtaaca tattgacaag cgtaataaac tagaaatgaa atagtggttt 720 actatctttt cacagaactc tatcatataa tatatgtaag cagttttctc atattttggt 780 ctaagtctct aacatcatca acattgttga actctcattt catgatctcc caaactgttc 840 ctgtagcctt atgtttttca cccattgatg taggaataaa taacaaacac tcttatcctt 900 aactaatctt agataaacat ccattaaact caacaaatcc aaaagataaa taatctagtg 960 ttaccttttt cccaattcct cctcccctta cttcgtttaa acttatgtgt ttctttagaa 1020 attactcaaa accatatact agcatatcta caatccaatt aaactgtata actgtttaat 1080 tcctaaagcc atggacacaa attaatttga atttataaaa gataagttct tacagataac 1140 aaacaaaatt ggaaactgaa aaaaaagttc aatagactat agagaatgtg attacatttt 1200 gtaaatgttt tttttttcca tatttgaaaa tgcttattta tagagtaata attattttta 1260 ttctctcctc tccctctaat ccctatcgct tctttttctt tcacttctcc tcttctccat 1320 tttttcttct ttctccattt ctgcttattt tttaagcaat acttaagagc agtttaagaa 1380 gtacttaacc acctaatcta tgattaatat atactaataa aagtttaact caattaacaa 1440 tatttaccat agagtttaat ttcctgcatc catactttaa atattaagaa gaaaaaaaac 1500 cttaaaacct tccttgctat attttcttgt tttcttcatc agcttcattc ttcttcgtgt 1560 ttataaattt tcttccttct tcttcatttc ttttctcttt ccaattcacc ggacttatga 1620 aaaccgacaa aacccaccct atcatcttct tgattcacgc tattgcagtt cccaatgttg 1680 gcacggaact caataaatgg tggattgggt tgttatcttt ttcgcgtcat cagctattat 1740 tttccatttg gatagaatac cgccatcaac ctccataacg ataatcttca acattcctat 1800 cctactcaca aaatgtgctt catatttata cggacaccta acctctctac agacccaccg 1860 attacatttc acttgaggaa caacacagaa ccaagatggg atcgcttggg atgtattccg 1920 aatcaggatt aacgaagaag ggtagcagca gaggctacga cgttccagag ggagtggaca 1980 ttcgaggacg ctatgatgaa gaatttgcca aaattctcaa caaggaagcc ttgttgttta 2040 tcgctgattt acagagaact tttagaaacc acatcaagta ttcgatggaa tgccgcagag 2100 aagccaagag gcggtataat gaaggggggt taccggggtt tgatccggcg actaagtata 2160 taagagattc tgagtggaca tgtgcccctg tccccccagc agttgctgat cggagagtgg 2220 agatcaccgg acctgtggag cggaagatga tcatcaacgc actcaattct ggagctaaag 2280 ttttcatggt ctgttctctc ttccttctct ctcctaaaga ctttgttttt atttaagatc 2340 ttgaaatgtc tggagtatgt tgtctgtccc actgcccctc agaacaatca aaaacttgtt 2400 ctttttaata acaaaccgtt tgtagacagt agacaggcat gagctcgagc tggtgtagtg 2460 ggatgaataa caattttagg gcaggtatta tttcagttat atatatatat atatatatta 2520 tattaaaagc atgaagtccg cgccatgggc ctatactaaa tcagtccgtg tagatagcac 2580 ttaaggcact gttatggaac taagtttcaa tgtctattct atgcaatttg catttatttg 2640 cttttcgtaa cgtggatttc atgacataaa agaatgaagt ttgatgaaaa aaccaatcaa 2700 aatgaaaaca ggcggacttt gaagatgcac tgtcaccaaa ttgggagaat ctgatgaggg 2760 ggcaaattaa tcttaaggat gcagtggatg ggactataag tttccatgat agagttagaa 2820 acagggttta taagctgaat gatcggacag ccaagctctt tgtccgccct cgaggttggc 2880 atttgccaga ggctcatatc ttcattgacg gcgagcctgc aaccggctgt cttgtggatt 2940 ttgggctcta ttttttccac aaccatgcta attttcgacg ctctcaaggt caaggttatg 3000 gccctttctt ttaccttccc aaaatggagc actccaggta aatcttcatc caaatattat 3060 tatgtaacca attgtgcaag ttttgagaaa ttgagctctg tttgcaatga gagtcgcttg 3120 tagatgatgc ataattgtcc ctgataaaat aggctacaag ataacattat gtaaagaatg 3180 aacacccctg gaaaatagac gttagtttga ggttaaaaac tactctgttt tgagaattag 3240 tctcgtgtct agaattaatg agttagttat cttattttct agccatttta gggagttttt 3300 aaaatatgat gttgttcttg gagagttctc tcaacttagg gatggacttt tcttgtttga 3360 ccatgaattt ggcctacatc acaatcttac gaacggtctg gtctaatatg tgatgtgtag 3420 ggaagctaaa atatggaaca gtgtatttga gagggcagag aagatggcag ggatagagag 3480 gggcagcatc agggccaccg tactaattga aacacttcca gcagtgtttc aaatgaatga 3540 aatactttac gagctgaggg atcactctgt gggattgaac tgtggtagat gggattacat 3600 attcagctat gtcaagacct tccaggctca cccagatcgt ctattacccg atcgggtcct 3660 agtcggtatg acccaacatt tcatgaggag ctattctgat cttctcatca ggacttgtca 3720 caggcgtggt gtgcatgcca tgggaggcat ggtaggtata atacaaacat tgcattcaat 3780 aatatttcac atccagagtt ttgacagcta gattcttttt gcaggctgct caaattccga 3840 ttagagacga tccgaaggca aatgaggtag cacttgagct agtgaggaag gacaaactga 3900 gggaggtaaa ggcagggcat gatggaacat gggcagcaca tccaggacta atcccggctt 3960 gtatggaagt cttcaccaac aacatgggga atgcccccaa ccaaattcga tccatgagac 4020 gtgacgatgc tgcaaacttg accgaagaag atctcttaca acagccaaga ggtgtacgta 4080 caatggaagg gcttcgcttg aacacccgag ttgggattca gtacctagcc gcatggctaa 4140 ccggggctgg ctcggtgcct ctatacaacc ttgcggaaga tgcagcaaca gcagaaatta 4200 gcagggttca aaactggcaa tggctgaagt atggagtgga attggatgga gatgggcttg 4260 gtgtgagagt gaacaaggaa ctgtttggga gagtggtgga agaagaaatg gaaaggattg 4320 aaagagaagt tgggaaagag agattcaaaa aaggaatgta caaagaagct tgcaagatgt 4380 tcacaaggca atgcacagct ccaaatttgg atgactttct gaccttagac gcttacaact 4440 atattgttat acatcatcca agggaattgt ccaagctttg aaaagtgaaa accaccacca 4500 aaacacttgc ttttgcttcc attctcttct tgttactgag aataacgtat ctgtaatgta 4560 agtctgtctt tgtccatttc tttttcaagc tgtgtggtta tttcaagaaa tccagcaatt 4620 gcaagcaaat tggaattcgg tacttctcta tatataaacc tatattttgc ctgttagctt 4680 atttgactct ctttgttcaa ctctcacatc agctatgttt cttatataat actatcgcat 4740 ttttcaattt gaaagtagat aagaagcata tttgtaattt aagtagggta aaagcgatta 4800 gagattatct atcataagct agtatggttt ggattttctg agtgaagaaa aaagaagatt 4860 atgtttgagt tcggattttc ctgctaaaga aaagaaaaag gttatatttg agtcagaaat 4920 tgatgatata gaacaccata ttttcttatt ttgcttaaat ttgttgcctc ccttgtaaaa 4980 ttcaccaact agactatatt tgttgtcacc atgacagaag aaaaaattgt tcaattcgaa 5040 atccaacttt aacaaaaaca gaacagaaaa agaatttatc aattatatgc tctctcatct 5100 catcatgcct taagcatcg 5119 // |
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