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EBI DbfetchID X13602; SV 1; linear; genomic DNA; STD; PRO; 4241 BP. XX AC X13602; XX DT 16-FEB-1989 (Rel. 18, Created) DT 14-NOV-2006 (Rel. 89, Last updated, Version 5) XX DE Caldocellum saccharolyticum celB gene for cellobiohydrolase/endocellulase XX KW cellobiohydrolase; endocellulase. XX OS Caldicellulosiruptor saccharolyticus OC Bacteria; Firmicutes; Clostridia; Thermoanaerobacterales; OC Thermoanaerobacterales Family III. Incertae Sedis; Caldicellulosiruptor. XX RN [1] RP 1-4241 RA Saul D.J.; RT ; RL Submitted (18-NOV-1988) to the EMBL/GenBank/DDBJ databases. RL Saul D.J., Department of Cellular and Molecular Biology, University of RL Auckland, Private Bag, Auckland, New Zealand. XX RN [2] RP 1-4241 RX DOI; 10.1093/nar/17.1.439 RX PUBMED; 2789517. RA Saul D.J.; RT "Nucleotide sequence of a gene from Caldocellum saccharolyticum encoding RT for exocellulase and endocellulase activity"; RL Nucleic Acids Res. 17(1):439-439(1989). XX FH Key Location/Qualifiers FH FT source 1..4241 FT /organism="Caldicellulosiruptor saccharolyticus" FT /mol_type="genomic DNA" FT /clone="pNZ1087" FT /db_xref="taxon:44001" FT promoter 623..628 FT /note="put. -35 region" FT promoter 646..651 FT /note="put. -10 region" FT RBS 661..667 FT /note="pot. rRNA binding site" FT CDS 679..3798 FT /transl_table=11 FT /product="celB polypeptide precursor" FT /db_xref="GOA:P10474" FT /db_xref="HSSP:1NBC" FT /db_xref="InterPro:IPR013781" FT /db_xref="UniProtKB/Swiss-Prot:P10474" FT /protein_id="CAA31936.1" FT /translation="MKRNLFRIVSRVVLIAFIASISLVGAMSYFPVETQAAPDWSIPSL FT CESYKDDFMIGVAIPARCLSNDTDKRMVLKHFNSITAENEMKPESLLAGQTSTGLSYRF FT STADAFVDFASTNKIGIRGHTLVWHNQTPDWFFKDSNGQRLSKDALLARLKQYIYDVVG FT RYKGKVYAWDVVNEAIDENQPDSYRRSTWYEICGPEYIEKAFIWAHEADPNAKLFYNDY FT NTEISKKRDFIYNMVKNLKSKGIPIHGIGMQCHINVNWPSVSEIENSIKLFSSIPGIEI FT HITELDMSLYNYGSSENYSTPPQDLLQKQSQKYKEIFTMLKKYKNVVKSVTFWGLKDDY FT SWLRSFYGKNDWPLLFFEDYSAKPAYWAVIEASGVTTSSPTPTPTPTVTVTPTPTPTPT FT PTVTATPTPTPTPVSTPATGGQIKVLYANKETNSTTNTIRPWLKVVNSGSSSIDLSRVT FT IRYWYTVDGERAQSAVSDWAQIGASNVTFKFVKLSSSVSGADYYLEIGFKSGAGQLQPG FT KDTGEIQIRFNKSDWSNYNQGNDWSWLQSMTSYGENEKVTAYIDGVLVWGQEPSGATPA FT PTMTVAPTATPTPTLSPTVTPTPAPTQTAIPTPTLTPNPTPTSSIPDDTNDDWLYVSGN FT KIVDKDGRPVWLTGINWFGYNTGTNVFDGVWSCNLKDTLAEIANRGFNLLRVPISAELI FT LNWSQGIYPKPNINYYVNPELEGKNSLEVFDIVVQTCKEVGLKIMLDIHSIKTDAMGHI FT YPVWYDEKFTPEDFYKACEWITNRYKNDDTIIAFDLKNEPHGKPWQDTTFAKWDNSTDI FT NNWKYAAETCAKRILNINPNLLIVIEGIEAYPKDDVTWTSKSSSDYYSTWWGGNLRGVR FT KYPINLGKYQNKVVYSPHDYGPSVYQQPWFYPGFTKESLLQDCWRPNWAYIMEENIAPL FT LIGEWGGHLDGADNEKWMKYLRDYIIENHIHHTFWCFNANSGDTGGLVGYDFTTWDEKK FT YSFLKPALWQDSQGRFVGLDHKRPLGTNGKNINITTYYNNNEPEPVPASK" FT sig_peptide 679..762 FT /note="signal peptide" FT mat_peptide 763..3795 FT /note="mat. cellobiohydrolase/endocellulase" FT misc_feature 1804..1932 FT /note="TP box" FT misc_feature 2398..2532 FT /note="TP box" FT RBS 4024..4030 FT /note="pot. rRNA binding site" FT CDS 4043..4241 FT /partial FT /transl_table=11 FT /note="unkown ORF" FT /db_xref="GOA:P21481" FT /db_xref="HSSP:1G87" FT /db_xref="InterPro:IPR001701" FT /db_xref="UniProtKB/Swiss-Prot:P21481" FT /protein_id="CAA31937.1" FT /translation="MLKLKKAIKRITLCVAVVFLLQILFLFSGYNNSDVKAATTFNYGE FT ALQKAIMFYEFQMSGKLPSWI" XX SQ Sequence 4241 BP; 1524 A; 636 C; 920 G; 1161 T; 0 other; cggtgtatca catagtatta cgttagattt caatgtaagc ataaaaatta tacaaaatga 60 ggttataaaa ttcataagga atttggtttt tatgagggct ctggtgtaat gccaaagtgg 120 caattgataa tgtaacttgg acgtaagaat tgtgtcaaat atgttgatag tatttttgca 180 acagaggttg aaatattttc aaaatgagca ttgttaattt aaatagactg agagcacaaa 240 tgtgaagctt aaaatttgga aaaagagcga taattacaaa ataaggtttt taaaattaga 300 tactgtcgaa gaaaaaacag taactgagtt tttaatgatg ttaaattcta taaagtcaaa 360 tgtaccacat atggtatata tgaaattaac ataagaaaaa atgcaaaaaa gaataaatta 420 taatttaatt ataggtttaa aaaagccgaa aaaaatattt aagtttacgc tgcacgatgt 480 gctgggagcc tttcggcaac accagagcct ttgtttaaag ttttgctcat attaagaatt 540 ttttaatcac aaataagctt tcaaaaaatg gtaacatata gcgtctaaat tgaacaatta 600 tatagtgtcg ccaatttgtt tttcgacaaa atgtaaaata aaaaatttaa aaataagaag 660 agggggttag tggataaaat gaagagaaat ctatttagga ttgtatctcg agttgtatta 720 atagcgttta ttgctagtat ttctctggtt ggtgcaatga gttattttcc agttgaaact 780 caagctgcgc cagactggag tattccaagt ttatgtgaga gttataaaga cgattttatg 840 ataggcgtgg caatacctgc aagatgtttg agcaatgata ctgacaaacg aatggtattg 900 aaacatttta acagtataac agcagagaat gaaatgaagc cggaaagctt attagctggt 960 cagaccagta ctgggttaag ttatagattt agtactgctg atgcttttgt tgactttgcg 1020 agtacaaaca agataggtat aagaggtcat acattagttt ggcataatca gacacctgat 1080 tggttcttta aagatagcaa tggtcaaagg ttatccaaag atgcattatt agcaagacta 1140 aagcaatata tttatgatgt tgttggaaga tataaaggga aagtgtatgc atgggacgtt 1200 gtcaatgaag ctatcgatga gaatcagcca gatagttata gacgttcgac atggtatgaa 1260 atttgtggtc ctgagtacat tgaaaaagca tttatatggg ctcatgaagc agaccctaac 1320 gcaaagctat tctacaatga ctataatacc gagatttcta agaaaagaga ttttatatac 1380 aacatggtaa aaaacctgaa atctaaaggt atacctattc atggtattgg tatgcaatgt 1440 catataaatg ttaactggcc atctgttagc gaaatagaaa acagtattaa gttatttagt 1500 tcgataccag gtattgagat tcacattaca gaacttgata tgagcctata caactacggc 1560 tccagcgaaa attattccac accaccacag gatttgcttc aaaagcagtc tcagaaatac 1620 aaagagattt ttacaatgct gaaaaaatac aaaaatgtag taaaaagtgt tactttttgg 1680 ggattgaaag atgattattc atggttaaga tcattctacg gtaagaatga ttggccgttg 1740 ttgttttttg aagattacag tgcaaagcca gcctattggg cagtaattga agcttctggg 1800 gtaacaacat catctccgac acctacacct acgccgacag taacagtaac accaactcca 1860 acaccgacac cgacaccgac agtaacagcg actccaacac ctacacctac acctgttagc 1920 acacctgcga caggtgggca gataaaggta ctgtatgcta acaaggagac aaacagcacg 1980 acaaacacga taaggccatg gttgaaggta gtgaatagtg gcagcagtag catagatttg 2040 agcagggtaa cgataaggta ctggtacacg gtagatggtg aaagggcaca gagtgcggta 2100 tcagactggg cacagatagg agcaagcaat gtaacattca agtttgtgaa gctgagcagt 2160 agtgtaagtg gagcggatta ttacttggag ataggattta agagtggagc agggcagtta 2220 cagcctggga aggacacagg agagatacag ataaggttta acaagagtga ctggagcaat 2280 tacaatcagg ggaatgactg gtcatggtta cagagcatga cgagttatgg agagaatgag 2340 aaggtaacag cgtatataga tggagtgctg gtatggggac aggagccgag tggagcgacg 2400 cctgcgccga cgatgacggt tgcaccgaca gcgaccccga caccaactct gagtccgacg 2460 gtaacgccga cgccggcacc gacgcagaca gcaataccaa cgccaacatt aactccaaat 2520 ccaacgccaa catcgagtat tcctgatgat acaaatgatg attggcttta tgtaagtggt 2580 aataaaatag ttgataaaga cggtagacca gtatggttaa caggtattaa ctggtttgga 2640 tataatactg gtacaaatgt atttgatggt gtatggagtt gtaatctgaa agatactctt 2700 gctgaaatag ctaatagagg ctttaacttg ctaagagttc caatatctgc agagcttata 2760 ttgaactggt cgcaaggtat ttatccaaaa ccaaatataa actactacgt aaatccagag 2820 cttgaaggca aaaacagctt ggaagtattt gacatagtag tgcaaacatg taaagaagtt 2880 ggtttgaaaa ttatgttgga tattcacagt ataaaaacag acgcgatggg acatatctat 2940 ccggtatggt atgatgaaaa atttactcca gaggattttt ataaagcgtg cgaatggatt 3000 acaaatagat ataaaaatga tgacactatt atagcttttg acctaaagaa tgagccacat 3060 ggaaaaccgt ggcaagatac aacatttgca aaatgggata attcaacaga tattaataat 3120 tggaaatatg cagctgaaac atgtgcaaaa cgtatactga atataaatcc aaaccttcta 3180 attgtaattg aaggaattga agcctatcca aaggacgacg tcacatggac atcaaaatcc 3240 tccagcgact actattcaac atggtggggc ggtaacttgc gaggtgttag aaagtatcct 3300 attaatctgg gtaaatatca aaataaagtt gtatattcgc cacatgatta cgggccatct 3360 gtttaccagc agccgtggtt ttatccaggg ttcacaaaag agtctttact acaagattgt 3420 tggcgtccga attgggcgta cattatggaa gaaaacattg cgccacttct gataggtgag 3480 tggggtggtc atctggatgg agccgataac gaaaagtgga tgaagtattt acgagattat 3540 atcatagaaa atcatattca tcacacattt tggtgcttta atgctaactc gggtgacact 3600 ggaggattgg ttggatatga ttttacgaca tgggatgaga aaaaatactc atttttgaaa 3660 ccggctctat ggcaagacag tcaaggtagg tttgttggat tagatcacaa aagaccctta 3720 ggtacaaatg ggaaaaacat taatattaca acatattaca acaataatga gcctgagcca 3780 gttccagctt caaaatgata aaatacatta aataggaatt tatggatata tgagaaaaat 3840 ttaaggtcag taggggaatg tatagttaaa gtcattaagc aagcgaatta aaggaaaaag 3900 gggggtgata aaaaacggct aaaaacataa gtaattatta aacatttatt gtaggaacta 3960 agtataagtt tcaaaatcat accgtccaat acgattaatt gaagttaact atcaaatcaa 4020 aaagaggggg ttagggttaa aaatgcttaa actaaaaaaa gcaataaaaa ggataacact 4080 gtgtgttgct gtggtatttc tattgcagat tttatttcta ttttcaggat acaataacag 4140 tgatgtgaaa gcagcaacaa cctttaacta tggtgaagct cttcaaaaag caattatgtt 4200 ttatgaattc cagatgtcag gtaaactgcc atcatggatc c 4241 // |
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