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EBI DbfetchID X13520; SV 1; linear; genomic DNA; STD; PRO; 4175 BP. XX AC X13520; XX DT 18-JAN-1990 (Rel. 22, Created) DT 18-APR-2005 (Rel. 83, Last updated, Version 12) XX DE Rhodobacter capsulatus ORFX and hupL and hupS genes for hydrogenase large DE and small subunits XX KW hupL gene; hupS gene; hydrogenase; hydrogenase large subunit; KW hydrogenase small subunit; unidentified reading frame. XX OS Rhodobacter capsulatus OC Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; OC Rhodobacteraceae; Rhodobacter. XX RN [1] RP 490-3509 RX DOI; 10.1007/BF00340186 RX PUBMED; 3067084. RA Leclerc M., Colbeau A., Cauvin B., Vignais P.M.; RT "Cloning and sequencing of the genes encoding the large and the small RT subunits of the H2 uptake hydrogenase (hup) of Rhodobacter capsulatus"; RL Mol. Gen. Genet. 214(1):97-107(1988). XX RN [2] RP 3325-3405 RA Leclerc M., Colbeau A., Cauvin B., Vignais P.M.; RT "Cloning and sequencing of the genes encoding the large and the small RT subunits of the H(2) uptake hydrogenase (hup) of Rhodobacter capsulatus"; RL Mol. Gen. Genet. 215:368-368(1988). XX RN [3] RP 1-4175 RA Richaud P.; RT ; RL Submitted (07-MAR-1990) to the EMBL/GenBank/DDBJ databases. RL Richaud P., CNRS UA 1130, DRF/LBIO/Biochimie Microbienne, CEN-Grenoble - 85 RL X, 38041 Grenoble Cedex, France. XX RN [5] RP 1-4175 RX DOI; 10.1111/j.1365-2958.1993.tb01199.x RX PUBMED; 8497190. RA Colbeau A., Richaud P., Toussaint B., Caballero J., Elster C., Delphin C., RA Smith R.L., Chabert J., Vignais P.M.; RT "Organization of the genes necessary for hydrogenase expression in RT Rhodobacter capsulatus. Sequence analysis and identification of two hyp RT regulatory mutants"; RL Mol. Microbiol. 8(1):15-29(1993). XX FH Key Location/Qualifiers FH FT source 1..4175 FT /organism="Rhodobacter capsulatus" FT /strain="B10" FT /mol_type="genomic DNA" FT /clone_lib="pBR322" FT /clone="pAC70" FT /db_xref="taxon:1061" FT RBS 480..484 FT /note="put. ribosome binding site" FT CDS 490..1566 FT /transl_table=11 FT /product="hydrogenase small subunit preprotein (AA -45 to FT 313)" FT /db_xref="GOA:P15283" FT /db_xref="InterPro:IPR001821" FT /db_xref="InterPro:IPR006137" FT /db_xref="UniProtKB/Swiss-Prot:P15283" FT /protein_id="CAA31869.1" FT /translation="MSDIETFYDVMRRQGITRRSFMKFCSLTAAALGLGPSFVPKIAEA FT METKPRTPVVWVHGLECTCCSESFIRSAHPLAKDVVLSMISLDYDDTLMAAAGHAAEAA FT FEETIAKYKGNYILAVEGNPPLNEDGMFCITGGKPFVEKLRHAAEGAKAIISWGACASY FT GCVQAAAPNPTQATPVHKVITDKPIIKVPGCPPIAEVMTGVITYMLTFDRMPELDRQGR FT PAMFYSQRIHDKCYRRPHFDAGQFVEHWDDENARKGYCLYKMGCKGPTTYNACSTVPLE FT RRRHFPIQSGHGCIGCSEDGFWDQGSFYDRLTTIKQFGIEATADQIGWTATGLVGAAVA FT AHAAVSVLKRAQKKNEEA" FT sig_peptide 490..624 FT /note="put. signal peptide (AA -45 to -1)" FT mat_peptide 625..1563 FT /note="hydrogenase small subunit (AA 1 - 313)" FT RBS 1556..1561 FT /note="put. ribosome binding site" FT CDS 1570..3363 FT /transl_table=11 FT /note="hydrogenase large subunit (AA 1 - 617)" FT /db_xref="GOA:P15284" FT /db_xref="InterPro:IPR001501" FT /db_xref="InterPro:IPR018194" FT /db_xref="UniProtKB/Swiss-Prot:P15284" FT /protein_id="CAA31870.1" FT /translation="MTTQTPNGFTLDNAGKRIVVDPVTRIEGHMRCEVNVNDQGIITNA FT VSTGTMWRGLEVILKGRDPRDAWAFTERICGVCTGTHALTSVRAVESALGITIPDNANS FT IRNMMQLNLQIHDHIVHFYHLHALDWVNPVNALRADPKATSELQQMVSPSHPLSSPGYF FT RDVQNRLKKFVESGQLGLFKNGYWDNPAYKLPPEADLMATTHYLEALDLQKEVVKVHTI FT FGGKNPHPNWLVGGVPCPINVDGVGAVGAINMERLNLVSSIIDRCTEFTRNVYLPDLKA FT IGGFYKEWLYGGGLSGQSVLSYGDIPENPNDFSAGQLHLPRGAIINGNLNEVHDVDTTD FT PEQVQEFVDHSWYDYGEPGMGLHPWDGRTEPKFELGPNLKGTRTNIENIDEGAKYSWIK FT APRWRGNAMEVGPLAATSSVTRKGHEDIKNQVEGLLRDMNLPVSALFSTLGRTAARALE FT AEYCCRLQKHFFDKLVTNIKNGDSSTANVEKWDPSTWPKEAKGVGMTEAPRGALGHWVK FT IKDGRIENYQCVVPTTWNGSPRDSKGNIGAFEASLLNTKMERPEEPVEILRTLHSFDPC FT LACSTHVMSAEGAPLTTVKVR" FT CDS 3373..4161 FT /transl_table=11 FT /note="ORF X" FT /db_xref="GOA:P16145" FT /db_xref="InterPro:IPR000516" FT /db_xref="InterPro:IPR011577" FT /db_xref="InterPro:IPR016174" FT /db_xref="UniProtKB/Swiss-Prot:P16145" FT /protein_id="CAA31871.1" FT /translation="MKGVSDERINAPVRGPDEIFEASRLTGDATREDLESIRRRTSVYV FT YEAPVRVWHWVNALAITILVVTGYFIASPLPSMQIGEATDQFVMGYIRFAHFAAGVVMS FT VAFFGRIYWAFVGNRHAWQMFYIPIFNKRYWKEFVFELRWYFFLEEEPKKYIGHNPLAH FT AAMFTFITLGITFMMITGWALYAEGAGQGGVTDSLFGWVLGYVQNSQRLHTLHHLGMWA FT IVIFAIIHIYAAVREDVMSRQSMVSTMISGHRTFKDDRIE" XX SQ Sequence 4175 BP; 847 A; 1316 C; 1283 G; 729 T; 0 other; ctgtttcaac ttctggcggc aggcgagcgc cccgaccgtg tcgcccatgc gctgcacgcc 60 agccttgcgc aggcttttgc cgccgaagcg cggcggctga tcgaagccgg acaggccgag 120 gcggtggcgc tgacgggcgg ctgttttcag aattccagac ttgcaaccat gacgcgaaat 180 ttccttgccg atcaaggcat tctgacgcag ggccgcatcc cggccaacga tgggggcctt 240 gccctgggtc aggcccttgt cgcggcggca aaattggaaa gcaactgacc gaagtggaaa 300 tggaatcggt caaaatactt tcggtttttt tacgtgacaa aatcacacac cattgaaaaa 360 caaggaaaaa tttttctgcg acaatttgcc gtttctcatt ggcggaaacc ggcccaacca 420 tgagaattcc actctaccca tgggcgcgcc gaggaggtca cggcgcgtca caaggcaagg 480 gagggacaat tgtcggacat cgaaactttc tatgacgtga tgcggcgtca ggggatcacc 540 cggcgcagct tcatgaagtt ctgttcgctc accgcagcag ctttggggct tgggccctcc 600 ttcgtgccga aaatcgccga agccatggaa acgaagccgc gcaccccggt cgtctgggtg 660 catgggcttg aatgcacctg ctgttcggaa agcttcatcc gctcggcgca tccgctggcc 720 aaggatgtcg tgctctcgat gatctcgctc gattacgacg acacgctgat ggccgccgcc 780 ggtcacgccg ccgaagcggc cttcgaggaa accatcgcca aatacaaggg caactacatc 840 ctggccgtcg agggcaaccc gccgctcaac gaagacggga tgttctgcat caccggcggc 900 aagccctttg tcgagaagct gcgccacgcc gccgagggcg ccaaggcgat catcagctgg 960 ggggcctgtg cgtcttatgg ctgcgtgcag gccgcggcgc cgaaccccac ccaggcgacg 1020 ccggtgcaca aggtgatcac cgacaagccg atcatcaagg tcccgggctg cccgccgatc 1080 gccgaggtga tgaccggcgt catcacctac atgctgacct tcgaccggat gccggaactg 1140 gaccgtcagg gccgcccggc gatgttctac agccagcgca tccacgacaa atgctaccgc 1200 cgcccgcatt tcgacgccgg gcaattcgtc gaacactggg acgacgaaaa cgcgcgcaag 1260 ggctattgcc tctacaagat gggctgcaag ggcccgacca cctacaacgc ctgttcgacc 1320 gtgccgctgg aacggcggcg tcacttcccg atccagtccg gtcacggctg catcggctgt 1380 tccgaggacg ggttctggga tcagggcagc ttctatgacc ggctgaccac gatcaagcaa 1440 ttcggcatcg aggccacggc cgaccagatc ggctggaccg ccaccggcct tgtgggcgcc 1500 gccgtcgccg cccatgccgc ggtctccgtg ctcaaacgcg cgcagaaaaa gaacgaggag 1560 gcgtaagcca tgacgaccca aacgccgaac ggcttcaccc tcgacaacgc cggcaagcgc 1620 atcgtcgtcg atcccgtcac ccggatcgaa ggccacatgc gctgcgaagt gaacgtcaac 1680 gatcagggca tcatcaccaa cgccgtctcg accgggacga tgtggcgcgg ccttgaagtg 1740 atcctgaagg gccgcgatcc gcgcgacgcc tgggccttca ccgaacggat ctgcggtgtc 1800 tgcaccggca cccatgcgct gacctcggtc cgcgcggtcg aaagcgcgct ggggatcacc 1860 atccccgaca atgcgaattc gatccgcaac atgatgcagc tgaacctgca gatccacgac 1920 catatcgtgc atttctacca cctgcatgcg ctggattggg tgaacccggt caatgcgctg 1980 cgcgccgatc cgaaggcgac ctcggaactg cagcagatgg tttcgcccag ccatccgctg 2040 tcgtcccccg gctatttccg cgacgtgcag aaccggctga agaaattcgt cgaatccggg 2100 cagctggggc tgttcaagaa cggctactgg gacaatccgg cctacaagct gccgcccgaa 2160 gccgatctga tggcgacgac gcattatctg gaagcgctgg atctgcaaaa ggaagtggtc 2220 aaggtccaca cgatcttcgg cggcaagaac ccgcatccga actggcttgt gggcggtgtg 2280 ccctgtccga tcaacgtcga tggcgtgggc gcggtcggtg cgatcaacat ggagcggctg 2340 aacctcgtct cctcgatcat cgaccgctgc accgaattca cccgcaacgt ctatctgccc 2400 gacctcaagg ccatcggcgg cttctacaag gaatggctct atggcggcgg gctgtcgggg 2460 caatcggtgc tgtcctatgg cgacatcccg gaaaacccga atgatttcag cgccggtcag 2520 ctgcatctgc cgcgcggggc gatcatcaac ggcaacctga acgaggtgca tgacgtcgac 2580 acgaccgacc ccgaacaggt gcaggaattc gtcgaccatt cctggtatga ttacggcgag 2640 ccgggcatgg ggctgcatcc ctgggacggc cggaccgagc cgaaattcga gctgggcccg 2700 aacctgaaag gcacccgcac caacatcgag aacatcgacg aaggcgcgaa atattcctgg 2760 atcaaggcgc cgcgctggcg cggcaatgcg atggaggtgg ggccgctggc cgctacatcg 2820 tcggttacgc gcaagggcca cgaggacatc aagaaccagg tcgagggcct gctgcgcgac 2880 atgaacctgc cggtttcggc gctgttctcg acgctgggcc ggactgcggc gcgggctctg 2940 gaggcggaat actgctgccg tctgcaaaag cacttcttcg acaagctggt caccaacatc 3000 aagaacggcg acagctcgac cgcgaatgtc gagaaatggg atccctcgac ctggccgaag 3060 gaggccaagg gcgtgggcat gaccgaggcg ccgcgcggcg cgctgggcca ttgggtcaag 3120 atcaaggacg gccgcatcga gaactatcaa tgcgtcgtgc cgacgacctg gaacggctcg 3180 ccccgcgaca gcaagggcaa tatcggcgcc ttcgaggcct cgctgctgaa cacgaagatg 3240 gaacgccccg aagagccggt cgagatcctg cgcacgctgc acagcttcga tccgtgcctg 3300 gcctgttcga cgcatgtgat gtcggccgaa ggcgcccccc tgaccaccgt caaggtccgg 3360 tagggggatg ccatgaaggg agtttccgac gaaaggatca atgcccccgt ccgtggcccg 3420 gatgaaatct tcgaggcctc gcgactgacc ggcgacgcca cccgcgagga cctagaaagc 3480 atccggcggc gtacctccgt ctatgtctac gaggcgccgg tccgggtctg gcactgggtc 3540 aacgcgctgg cgatcaccat cctggtggtc accggctatt tcatcgcctc gccgctgccc 3600 tcgatgcaga tcggcgaggc caccgaccag ttcgtgatgg gctacatccg cttcgcgcat 3660 ttcgccgccg gggtggtgat gtcggtggcc tttttcggcc gcatctactg ggccttcgtg 3720 ggcaaccgcc acgcctggca gatgttctac atcccgatct tcaacaagcg ctactggaag 3780 gaattcgtct tcgagctgcg ctggtatttc ttccttgaag aagagccgaa gaaatacatc 3840 ggccacaacc cgctggcgca tgcggcgatg ttcaccttca tcaccttggg catcaccttc 3900 atgatgatca ccggctgggc gctttatgcc gaaggcgcgg ggcagggggg cgtgaccgac 3960 agcctgttcg gctgggtgct gggctatgtg caaaacagcc agcggctgca cacgttgcac 4020 catctgggca tgtgggcgat cgtgatcttc gcgatcatcc atatctacgc cgcggtgcgc 4080 gaagacgtga tgagccgcca gtcgatggtc tcgacgatga tctcggggca ccggaccttc 4140 aaggacgacc ggatcgagtg actggaaaga agctt 4175 // |
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