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EBI DbfetchID X13145; SV 2; linear; genomic DNA; STD; PRO; 7484 BP. XX AC X13145; XX DT 10-FEB-1989 (Rel. 18, Created) DT 03-JAN-2006 (Rel. 86, Last updated, Version 10) XX DE Escherichia Coli plasmid R124/3 hsdM, hsdS and hsdR genes for EcoR124I type DE I restriction and modification enzyme XX KW hsdM gene; hsdR gene; hsdS gene; plasmid; KW restriction and modification system type I. XX OS Escherichia coli OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales; OC Enterobacteriaceae; Escherichia. OG Plasmid R124/3 XX RN [1] RC revised by [3] RA Bickle T.A.; RT ; RL Submitted (09-OCT-1988) to the EMBL/GenBank/DDBJ databases. RL Bickle T.A., Biozentrum , Basel University, Klingelbergstrasse 70, CH-4056 RL Basel, Switzerland. XX RN [2] RX DOI; 10.1016/0022-2836(89)90369-0 RX PUBMED; 2784505. RA Price C., Lingner J., Bickle T.A., Firman K., Glover S.W.; RT "Basis for changes in DNA recognition by the EcoR124 and EcoR124/3 type I RT DNA restriction and modification enzymes"; RL J. Mol. Biol. 205(1):115-125(1989). XX RN [3] RC revised by [4] RA Bickle T.A.; RT ; RL Submitted (13-JUL-1993) to the EMBL/GenBank/DDBJ databases. RL Bickle T.A., Biozentrum , Basel University, Klingelbergstrasse 70, CH-4056 RL Basel, Switzerland. XX RN [4] RP 1-7484 RA Blundell A.; RT ; RL Submitted (16-NOV-2005) to the EMBL/GenBank/DDBJ databases. RL Blundell A., King Henry Building, King Henry I St. Portsmouth, Hants, PO5 RL 2DY, UK XX FH Key Location/Qualifiers FH FT source 1..7484 FT /organism="Escherichia coli" FT /plasmid="R124/3" FT /strain="Plasmid R124/3" FT /mol_type="genomic DNA" FT /db_xref="taxon:562" FT misc_feature 365..365 FT /note="transcription initiation site" FT CDS 390..1952 FT /transl_table=11 FT /note="hsdM protein (AA 1-520)" FT /db_xref="GOA:P10484" FT /db_xref="InterPro:IPR002052" FT /db_xref="InterPro:IPR002296" FT /db_xref="InterPro:IPR003356" FT /db_xref="InterPro:IPR004546" FT /db_xref="UniProtKB/Swiss-Prot:P10484" FT /protein_id="CAA31541.1" FT /translation="MKMTSIQQRAELHRQIWQIANDVRGSVDGWDFKQYVLGALFYRFI FT SENFSSYIEAGDDSICYAKLDDSVITDDIKDDAIKTKGYFIYPSQLFCNVAAKANTNDR FT LNADLNSIFVAIESSAYGYPSEADIKGLFADFDTTSNRLGNTVKDKNARLAAVLKGVEG FT LKLGDFNEHQIDLFGDAYEFLISNYAANAGKSGGEFFTPQHVSKLIAQLAMHGQTHVNK FT IYDPAAGSGSLLLQAKKQFDNHIIEEGFFGQEINHTTYNLARMNMFLHNINYDKFDIKL FT GNTLTEPHFRDEKPFDAIVSNPPYSVKWIGSDDPTLINDERFAPAGVLAPKSKADFAFV FT LHALNYLSAKGRAAIVCFPGIFYRGGAEQKIRQYLVDNNYVETVISLAPNLFFGTTIAV FT NILVLSKHKTDTNVQFIDASELFKKETNNNILTDAHIEQIMQVFASKEDVAHLAKSVAF FT ETVVANDYNLSVSSYVEAKDNREIIDIAELNAELKTTVSKIDQLRKDIDAIVAEIEGCE FT VQK" FT CDS 1949..3163 FT /transl_table=11 FT /note="hsdS protein (AA 1-410)" FT /db_xref="GOA:P10485" FT /db_xref="InterPro:IPR000055" FT /db_xref="UniProtKB/Swiss-Prot:P10485" FT /protein_id="CAB37630.2" FT /translation="MSEMSYLEKLLDGVEVEWLPLGEITKYEQPTKYLVKAKDYHDTYT FT IPVLTAGKTFILGYTNETHGIYQASKAPVIIFDDFTTANKWVDFDFKAKSSAMKMVTSC FT DDNKTLLKYVYYWLNTLPSEFAEGDHKRQWISNYSQKKIPIPCPDNPEKSLAIQSEIVR FT ILDKFTALTAELTAELNMRKKQYNYYRDQLLSFKEGEVEWKTLGEIGKWYGGGTPSKNK FT IEFWENGSIPWISPKDMGRTLVDSSEDYITEEAVLHSSTKLIPANSIAIVVRSSILDKV FT LPSALIKVPATLNQDMKAVIPHENILVKYIYHMIGSRGSDILRAAKKTGGSVASIDSKK FT LFSFKIPVPNINEQQRIVEILDKFDTLTNSITEGLPREIELRQKQYEYYRDLLFSFPKP FT ETVSN" FT CDS 3285..6386 FT /transl_table=11 FT /note="hsdR protein (AA 1-1033)" FT /db_xref="GOA:P10486" FT /db_xref="InterPro:IPR004473" FT /db_xref="InterPro:IPR006935" FT /db_xref="InterPro:IPR007409" FT /db_xref="InterPro:IPR014001" FT /db_xref="InterPro:IPR014021" FT /db_xref="UniProtKB/Swiss-Prot:P10486" FT /protein_id="CAA31543.1" FT /translation="MTHQTHTIAESNNFIVLDKYIKAEPTGDSYQSESDLERELIQDLR FT NQGYEFISVKSQSAMLANVREQLQNLNGVVFNDSEWRRFTEQYLDNPSDGILDKTRKIH FT IDYICDFIFDDERLENIYLIDKKNLMRNKVQIIQQFEQAGSHANRYDVTILVNGLPLVQ FT IELKKRGVAIREAFNQIHRYSKESFNSENSLFKYLQLFVISNGTDTRYFANTTKRDKNS FT FDFTMNWAKSDNTLIKDLKDFTATCFQKHTLLNVLVNYSVFDSSQTLLVMRPYQIAATE FT RILWKIKSSFTAKNWSKPESGGYIWHTTGSGKTLTSFKAARLATELDFIDKVFFVVDRK FT DLDYQTMKEYQRFSPDSVNGSENTAGLKRNLDKDDNKIIVTTIQKLNNLMKAESDLPVY FT NQQVVFIFDECHRSQFGEAQKNLKKKFKRYYQFGFTGTPIFPENALGSETTASVFGREL FT HSYVITDAIRDEKVLKFKVDYNDVRPQFKSLETETDEKKLSAAENQQAFLHPMRIQEIT FT QYILNNFRQKTHRTFPGSKGFNAMLAVSSVDAAKAYYATFKRLQEEAANKSATYKPLRI FT ATIFSFAANEEQNAIGEISDETFDTSAMDSSAKEFLDAAIREYNSHFKTNFSTDSNGFQ FT NYYRDLAQRVKNQDIDLLIVVGMFLTGFDAPTLNTLFVDKNLRYHGLMQAFSRTNRIYD FT ATKTFGNIVTFRDLERSTIDAITLFGDKNTKNVVLEKSYTEYMEGFTDAATGEAKRGFM FT TVVSELEQRFPDPTSIESEKEKKDFVKLFGEYLRAENILQNYDEFATLKALQQIDLSDP FT VAVEKFKAEHYVDDEKFAELQTIRLPADRKIQDYRSAYNDIRDWQRREKEAEKKEKSTT FT DWDDVVFEVDLLKSQEINLDYILGLIFEHNRQNKGKGEMIEEVKRLIRSSLGNRAKEGL FT VVDFIQQTNLDDLPDKASIIDAFFTFAQREQQREAEALIKEENLNEDAAKRYIRTSLKR FT EYATENGTELNETLPKLSPLNPQYKTKKQAVFRKSSRLLRSLKA" FT misc_feature 6559..7327 FT /note="IS1 element" XX SQ Sequence 7484 BP; 2235 A; 1655 C; 1696 G; 1898 T; 0 other; aaccagttcc ttgttggtgg agtccagggt gtcaaacagg gatgcaaatt cagcatctag 60 cgccgctttt ttgtaggctt cgaaagtagc cttgctgaca attactgctg gctcacggcc 120 tctgcgggtg atttcaacct cttccccggc ctcaacattg ttgagcactt cagaaaggtt 180 gccgcgcgcg gtacggaagt taatggattg cataaacacc tcgtgtactc gttatgtgta 240 cacaattata aacttcacag gcataaagca ccagcacttt gcggcttaac aaacctctag 300 gcaggtcatt cgtagcctaa tgtccgaact gctaaagcat ccaagttgct gtagaatcac 360 cgccaattac ataagcctga aataagtgga tgaaaatgac aagtattcaa caacgtgcag 420 agcttcatcg tcaaatctgg caaattgcta acgatgtcag gggttcggtc gatggatggg 480 attttaagca atacgttctg ggcgcacttt tctaccgttt tatcagcgaa aatttttcca 540 gttatattga agccggtgat gacagtatct gttatgcgaa actggatgac agcgtaatta 600 ctgatgacat taaagacgat gccatcaaaa ccaaaggcta cttcatctac ccaagccagc 660 ttttctgcaa cgtagctgcg aaagcaaata ccaatgacag attgaatgca gatttaaaca 720 gtatcttcgt tgctatcgaa agttctgctt acggttatcc ttcagaagct gacatcaaag 780 gtttgtttgc tgatttcgat accaccagta accgcctggg taacaccgtt aaagataaaa 840 atgcccgcct ggctgcggtt ctgaaagggg ttgaagggtt aaaacttggt gacttcaacg 900 aacatcagat tgacctgttc ggtgatgcct atgagttcct gatttctaac tatgcggcaa 960 atgccggtaa gtcaggcggt gagttcttta caccgcagca cgtctctaag ctgattgcac 1020 aactggcaat gcatggccag acccacgtta acaaaatcta cgacccggcc gcaggttccg 1080 gttcgctgtt gttgcaggct aaaaagcagt ttgataacca tatcattgaa gaaggctttt 1140 tcggacaaga aatcaaccat acgacctata acctggcacg tatgaacatg tttttgcaca 1200 acatcaacta cgacaagttt gatatcaagc tgggcaatac gctgactgag ccgcacttca 1260 gagatgaaaa accgtttgat gccatcgttt ctaacccgcc gtattcggtg aaatggattg 1320 gcagcgatga cccgacgctg attaacgatg agcgttttgc cccggctggc gttctggccc 1380 ccaaatccaa agctgacttt gcgtttgtat tacatgcact gaactatctt tctgccaaag 1440 gtcgtgctgc gattgtctgc ttcccgggta ttttttaccg tggcggcgcg gagcagaaaa 1500 tccgtcaata tctggtcgac aataactatg tcgaaaccgt aatttcactg gcaccgaacc 1560 tgttctttgg caccaccatt gcagtaaaca ttctggttct gtccaaacat aaaacggata 1620 ccaacgttca gtttattgac gccagcgaac tgttcaaaaa agagactaac aacaatatcc 1680 tgaccgatgc ccatatcgaa cagattatgc aggtatttgc cagcaaggaa gatgttgctc 1740 atctggcgaa atctgtcgcg tttgagaccg ttgttgctaa tgactataac ctgtcggtga 1800 gcagctatgt ggaagcgaaa gataaccgcg aaattatcga tattgctgag ctgaatgctg 1860 agctgaaaac cacggtcagc aaaatcgacc agttgcgcaa agatattgat gcgattgtgg 1920 ctgaaattga aggctgcgag gtgcagaaat gagcgagatg agttatctgg aaaaattgct 1980 ggatggggtt gaagttgagt ggttgccatt gggggaaata accaaatatg aacagccaac 2040 aaagtatttg gtaaaagcaa aagattatca cgacacatat accataccgg ttcttactgc 2100 cggaaaaaca tttattcttg gatacacaaa tgagactcat ggtatttatc aggcatcaaa 2160 ggcacctgta ataatttttg atgatttcac caccgctaat aaatgggtgg attttgactt 2220 taaggcaaaa tcatcggcca tgaaaatggt tacatcatgt gatgataata aaaccttact 2280 gaaatatgtt tactattggc tcaatacatt accaagtgaa ttcgcagagg gagaccataa 2340 acgccaatgg attagtaatt attctcaaaa aaaaatccca atcccctgcc cggataatcc 2400 ggaaaaatcc cttgccatcc agtctgaaat cgttcggatt ctggataaat ttactgcact 2460 taccgctgag cttaccgctg agcttaacat gcgtaaaaaa cagtacaact actatcgcga 2520 ccagttgttg agttttaaag agggtgaggt tgagtggaag actttgggag agatcggaaa 2580 atggtatggg ggaggtacac catcaaaaaa taaaattgaa ttttgggaaa atggaagcat 2640 tccatggatt tccccgaaag atatgggccg aacacttgta gactcttcgg aagactatat 2700 aactgaggag gctgttctac attcgtcaac aaaactgatt ccagcaaact cgattgcaat 2760 cgttgttcgc tcaagcatat tggataaagt attaccaagt gcattaataa aagtacctgc 2820 tacgctcaat caagatatga aagcagtcat tcctcatgaa aacatcttgg ttaaatatat 2880 ttatcacatg attgggtcta gggggagtga tattctgcga gcagcgaaaa agacaggagg 2940 gtccgtagct tctattgaca gtaaaaaatt attttcattt aaaattccgg ttcctaacat 3000 caatgaacaa caacgaattg tcgaaatact cgacaaattc gacacactga ctaactccat 3060 caccgaaggt cttccgcgtg aaatcgagtt gcgccagaaa caatacgagt actatcgtga 3120 tttactgttc agtttcccga aacctgaaac tgtcagtaat taattgatca ttgctaccga 3180 tcggaccacc ttaacacccg gtcagtatat agactatttt ttacgcgccg gaagtcacca 3240 ttacccccct tccggccctt gccagacggc acaaaggatg cgctatgact catcagacac 3300 acaccattgc tgaatccaat aactttatcg ttcttgataa gtacatcaaa gctgagccaa 3360 caggcgacag ctaccagagc gaatcggacc tggaacgcga actgatacag gacctacgga 3420 atcagggcta tgaatttata tccgtaaaat cacagtcggc gatgctggcc aatgttcggg 3480 aacagcttca gaacctcaat ggtgtggtgt ttaatgacag cgagtggcgg cgtttcacgg 3540 agcagtatct ggataacccg agcgatggca ttctggataa gacccgtaaa atccatatcg 3600 actatatttg cgactttatt tttgatgacg agcgtcttga gaacatctat ttgatagata 3660 aaaagaatct catgcgcaat aaggtgcaga ttatccagca gtttgaacag gcgggttctc 3720 acgctaaccg ttatgacgta actatcctgg ttaatggctt accgctggta caaatcgaac 3780 tgaaaaaacg cggcgtggcg attcgtgagg ctttcaacca gatacatcgt tacagtaaag 3840 agagttttaa cagcgaaaat tccctgttta agtatctgca gctgttcgtc atttccaacg 3900 gcactgatac ccgttatttt gccaacacga caaagcgcga taaaaacagt tttgacttca 3960 ccatgaactg ggcgaaatca gacaatacgc tgattaaaga cctcaaagac tttaccgcca 4020 cctgtttcca gaaacatact ctgcttaatg ttctggtgaa ctacagcgtt tttgacagca 4080 gtcagacact actggtaatg cgaccgtacc agattgccgc caccgagcgc attctgtgga 4140 aaattaagag ttcctttaca gcgaagaact ggtcaaaacc ggaaagcggt gggtatatct 4200 ggcacactac cggttctggt aaaaccctca ccagctttaa agccgcgcgt ctggcaacag 4260 agctggactt tattgataaa gtcttctttg tggtcgacag gaaagacctc gattaccaga 4320 ccatgaagga atatcagcgt ttttcgccag acagcgtcaa cggctcggaa aataccgcag 4380 gccttaaacg aaatctggat aaggacgata acaaaattat cgtcactact attcagaaac 4440 tcaataacct gatgaaagca gaaagcgacc tgcctgtata taatcagcaa gtggtgttta 4500 tatttgatga atgccaccgc agccagtttg gagaagcgca gaaaaacctg aagaagaaat 4560 tcaaacgcta ttatcagttt ggttttaccg gcacccctat tttcccggaa aacgccttag 4620 gctcagaaac aaccgccagc gtatttggtc gtgaattgca ttcgtatgta attaccgatg 4680 cgattcgtga cgaaaaagtg ctcaaattca aggtggacta caacgatgtg cggccacagt 4740 ttaagtcttt agagacagaa actgacgaga aaaaactgag tgcggctgaa aatcagcagg 4800 cgtttcttca tcccatgcgt attcaggaaa tcacgcaata tattctgaat aatttccgcc 4860 agaaaaccca ccgtaccttc cctggctcaa aaggttttaa tgctatgttg gcagtgagca 4920 gcgtggatgc cgcgaaagcc tattatgcga cgtttaaacg gttacaagaa gaagccgcta 4980 ataaatcggc tacctataaa ccgctgcgta ttgcgacaat cttctccttt gccgccaatg 5040 aagaacaaaa tgccattggt gaaatttccg atgaaacttt tgataccagc gcaatggaca 5100 gcagtgctaa agagtttctg gacgctgcaa ttcgtgaata taacagccat tttaaaacta 5160 actttagcac cgacagtaac ggttttcaga actactatcg tgatttagcc cagcgggtta 5220 aaaatcagga tatcgatctg ctaattgtcg tggggatgtt cttaaccggc ttcgatgctc 5280 caacattgaa cacgctattc gtcgataaaa acttacgtta tcacggcctg atgcaggcat 5340 tttcccgcac caaccgcatt tatgacgcca ctaaaacctt cggtaatatc gtcactttcc 5400 gggatctgga acgctcaact attgatgcca taacgctgtt tggtgacaaa aacaccaaaa 5460 atgtggtgtt agaaaagagt tatacagagt atatggaagg ctttactgat gctgctacag 5520 gtgaagctaa acgcggcttc atgacagtag tttcagaact ggaacaacgg ttccctgacc 5580 ctaccagtat tgaaagtgaa aaagagaaga aagacttcgt taaactgttt ggcgaatacc 5640 tgcgtgccga gaacatcctg caaaactatg atgaatttgc cacgctaaaa gccctgcaac 5700 aaatcgatct tagcgatcct gttgcggtag aaaaattcaa agcagaacat tatgtggatg 5760 atgaaaagtt cgctgaattg caaacaattc gtctccctgc tgatcgcaag attcaggatt 5820 atcgttctgc ctataacgat attcgcgact ggcagcgccg tgagaaagaa gctgagaaaa 5880 aagagaaatc aaccactgac tgggatgacg tagtttttga ggtcgatttg ctgaagtctc 5940 aggaaataaa cctggattat atccttggac tgattttcga acacaacaga caaaataaag 6000 gcaagggcga aatgatcgaa gaggtcaaac gcttaattcg ttcaagcctg gggaaccgtg 6060 ctaaagaggg cctggtggtc gattttattc agcaaacgaa cctggatgat ttaccggaca 6120 aagccagcat cattgacgca ttctttacgt ttgctcaacg cgaacagcaa cgtgaagcag 6180 aagcattgat aaaagaagaa aatctcaatg aagatgcagc aaaacgctat attcgcacgt 6240 ctttaaaacg cgaatatgcc accgaaaatg gcacagaatt aaacgaaaca ttaccaaaac 6300 ttagtccgtt aaatccgcaa tataaaacga aaaaacaggc agttttcaga aaatcgtctc 6360 gtttattgag aagtttaaag gcgtaggcgg aaaaatatag cccaattcgt gtttttcttg 6420 cgggttctta attaaacccg caagagaccg tggggttcca aatggctaat atactcccct 6480 tacccatgag cgacgatgcc gccaaaagtg atagagaaca gccagaaata gatcgcggcc 6540 ataatgattt tgaatgccgg tgatgctgcc aacttactga tttagtgtat gatggtgttt 6600 ttgaggtgct cctgtggctt ctgtttctat cagctgtccc tcctgttcag ctactgacgg 6660 ggtggtgcgt aacggcaaaa gcactgccgg acatcagcgc tatctctgct ctcactgccg 6720 taaaacatgg caactgcagt tcacttacac cgcttctcaa cccggtacgc accagaaaat 6780 cattgatatg gccatgaatg gcgttggatg ccgggcaaca gcccgcatta tgggcgttgg 6840 cctcaacacg attttacgtc acttaaaaaa ctcaggccgc agtcggtaac ctcgcgcata 6900 cagccgggca gtgacgtcat cgtctgcgcg gaaatggacg aacagtgggg ctatgtcggg 6960 gctaaatcgc gccagcgctg gctgttttac gcgttatgac aggctccgga agacggtttt 7020 tgcgcacgta ttcggtgaac gcactatggc gacgctgggg cgtcttatga gcctgctgtc 7080 accctttgac gtggtgatat ggatgacgga tggctggccg ctgtatgaat cccgcctgaa 7140 gggaaagctg cacgtaatca gcaagcgata tacgcagcga attgagcggc ataacctgaa 7200 tctgaggcag cacctggcac ggctgggacg gaagtcgctg tcgttctcaa aatcggtgga 7260 gctgcatgac aaagtcatcg ggcattatct gaacataaaa cactatcagt aagttggagt 7320 cattacctca tgagagcatc aagagttgtc cttttaattt caaaggagct gaaatgttgt 7380 ttgcaatact gtgttactaa ccgtctgagg cgaaggtgac tatgggttta cgctgacact 7440 caggcaacca agaattgttt atcggatgtg ctccggcata cgta 7484 // |
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