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EBI DbfetchID X12868; SV 1; linear; genomic DNA; STD; UNC; 2665 BP. XX AC X12868; XX DT 29-JUN-1989 (Rel. 20, Created) DT 23-OCT-2008 (Rel. 97, Last updated, Version 10) XX DE Plasmid pLMO20 dhfrV gene for trimethoprim resistance XX KW antibiotic resistance; dhfrV gene; dihydrofolate reductase; plasmid; KW sulfonamide resistance; sulI gene; trimethoprim resistance. XX OS Plasmid pLMO20 OC other sequences; plasmids. XX RN [1] RP 1-2330 RX DOI; 10.1007/BF00339581 RX PUBMED; 3054482. RA Sundstroem L., Radstroem P., Swedberg G., Skoeld O.; RT "Site-specific recombination promotes linkage between trimethoprim- and RT sulfonamide resistance genes. Sequence characterization of dhfrV and sulI RT and a recombination active locus of Tn21"; RL Mol. Gen. Genet. 213(2-3):191-201(1988). XX RN [2] RP 1-2665 RA Sundstroem L.; RT ; RL Submitted (27-NOV-1989) to the EMBL/GenBank/DDBJ databases. XX FH Key Location/Qualifiers FH FT source 1..2665 FT /organism="Plasmid pLMO20" FT /host="Enterobacteriaceae" FT /mol_type="genomic DNA" FT /db_xref="taxon:88772" FT promoter 1035..1040 FT /note="-35 region" FT promoter 1058..1063 FT /note="-10 region" FT CDS complement(137..1150) FT /transl_table=11 FT /note="E2 gene product (AA 1-337)" FT /db_xref="GOA:P62590" FT /db_xref="InterPro:IPR002104" FT /db_xref="InterPro:IPR004107" FT /db_xref="InterPro:IPR010998" FT /db_xref="InterPro:IPR011010" FT /db_xref="InterPro:IPR011946" FT /db_xref="InterPro:IPR013762" FT /db_xref="UniProtKB/Swiss-Prot:P62590" FT /protein_id="CAA31355.1" FT /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAFI FT RFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQPLPALLFFYGKVLCTDLPWLQEI FT GRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDFDHG FT TIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALERKYPR FT AGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATPHTLRH FT SFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLTSER" FT promoter complement(1177..1182) FT /note="-10 region" FT promoter complement(1200..1205) FT /note="-35 region" FT misc_feature 1286..1289 FT /note="GTTA insertion branch point" FT RBS 1294..1298 FT /note="ribosome binding site" FT CDS 1306..1779 FT /transl_table=11 FT /note="dhfrV gene product (AA 1-157)" FT /db_xref="GOA:P11731" FT /db_xref="InterPro:IPR001796" FT /db_xref="InterPro:IPR012259" FT /db_xref="InterPro:IPR017925" FT /db_xref="UniProtKB/Swiss-Prot:P11731" FT /protein_id="CAA31356.1" FT /translation="MKVSLMAAKAKNGVIGCGPHIPWSAKGEQLLFKALTYNQWLLVGR FT KTFESMGALPNRKYAVVTRSAWTADNDNVIVFPSIEEAMYGLAELTDHVIVSGGGEIYR FT ETLPMASTLHISTIDIEPEGDVFFPNIPNTFEVVFEQHFSSNINYCYQIWQKG" FT repeat_region 1786..1807 FT /note="inverted repeat A" FT repeat_region 1829..1848 FT /note="inverted repeat A'" FT misc_feature 1854..1858 FT /note="GTTA insertion branch point" FT CDS 1963..2310 FT /transl_table=11 FT /note="E1 gene product (AA 1-115)" FT /db_xref="GOA:P0AA22" FT /db_xref="InterPro:IPR000390" FT /db_xref="UniProtKB/Swiss-Prot:P0AA22" FT /protein_id="CAA31357.1" FT /translation="MKGWLFLVIAIVGEVIATSALKSSEGFTKLAPSAVVIIGYGIAFY FT FLSLVLKSIPVGVAYAVWSGLGVVIITAIAWLLHGQKLDAWGFVGMGLIIAAFLLARSP FT SWKSLRRPTPW" FT RBS 2291..2295 FT /note="ribosome binding site" FT CDS 2304..>2665 FT /transl_table=11 FT /note="sulI gene product (AA 1-120)" FT /db_xref="GOA:P0C002" FT /db_xref="InterPro:IPR000489" FT /db_xref="InterPro:IPR006390" FT /db_xref="InterPro:IPR011005" FT /db_xref="UniProtKB/Swiss-Prot:P0C002" FT /protein_id="CAA31358.1" FT /translation="MVTVFGILNLTEDSFFDESRRLDPAGAVTAAIEMLRVGSDVVDVG FT PAASHPDARPVSPADEIRRIAPLLDALSDQMHRVSIDSFQPETQRYALKRGVGYLNDIQ FT GFPDPALYPDIAEADC" XX SQ Sequence 2665 BP; 596 A; 733 C; 742 G; 594 T; 0 other; ggatccatca ggcaacgacg ggctgctgcc ggccatcagc ggacgcaggg aggactttcc 60 gcaaccggcc gttcgatgcg gcaccgatgg ccttcgcgca ggggtagtga atccgccagg 120 attgacttgc gctgccctac ctctcactag tgaggggcgg cagcgcatca agcggtgagc 180 gcactccggc accgccaact ttcagcacat gcgtgtaaat catcgtcgta gagacgtcgg 240 aatggccgag cagatcctgc acggttcgaa tgtcgtaacc gctgcggagc aaggccgtcg 300 cgaacgagtg gcggagggtg tgcggtgtgg cgggcttcgt gatgcctgct tgttctacgg 360 cacgtttgaa ggcgcgctga aaggtctggt catacatgtg atggcgacgc acgacaccgc 420 tccgtggatc ggtcgaatgc gtgtgctgcg caaaaaccca gaaccacggc caggaatgcc 480 cggcgcgcgg atacttccgc tcaagggcgt cgggaagcgc aacgccgctg cggccctcgg 540 cctggtcctt cagccaccat gcccgtgcac gcgacagctg ctcgcgcagg ctgggtgcca 600 agctctcggg taacatcaag gcccgatcct tggagccctt gccctcccgc acgatgatcg 660 tgccgtgatc gaaatccaga tccttgaccc gcagttgcaa accctcactg atccgcatgc 720 ccgttccata cagaagctgg gcgaacaaac gatgctcgcc ttccagaaaa ccgaggatgc 780 gaaccacttc atccggggtc agcaccaccg gcaagcgccg cgacggccga ggtcttccga 840 tctcctgaag ccagggcaga tccgtgcaca gcaccttgcc gtagaagaac agcaaggccg 900 gcaatggctg acgatgcgtg gagaccgaaa ccttgcgctc gttcgccagc caggacagaa 960 atgcctcgac ttcgctgctg cccaaggttg ccgggtgacg cacaccgtgg aaacggatga 1020 aggcacgaac ccagtggaca taagcctgtt cggttcgtaa gctgtaatgc aagtagcgta 1080 tgcgctcacg caactggtcc agaaccttga ccgaacgcag cggtggtaac ggcgcagtgg 1140 cggttttcat ggcttgttat gactgttttt ttgtacagtc tatgcctcgg gcatccaagc 1200 agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc 1260 agcagggcag tcgccctaaa acaaagttaa cccggaacca aaattgtgaa agtatcatta 1320 atggctgcaa aagcgaaaaa cggagtgatt ggttgcggtc cacacatacc ctggtccgcg 1380 aaaggagagc agctactctt taaagccttg acgtacaacc agtggctttt ggtgggccgc 1440 aagacgttcg aatctatggg agcactccct aataggaaat acgcggtcgt tactcgctca 1500 gcctggacgg ccgataatga caacgtaata gtattcccgt cgatcgaaga ggccatgtac 1560 gggctggctg aactcaccga tcacgttata gtgtctggtg gcggggagat ttacagagaa 1620 acattgccca tggcctctac gctccatata tcgacgattg atattgagcc ggaaggagat 1680 gttttctttc cgaatattcc caataccttc gaagttgttt ttgagcaaca ctttagctca 1740 aacattaact attgctatca aatttggcaa aagggttaac aaagctatgc aattgacggt 1800 aaaaagcttc gttcgcttcg cttgctacgc ttcttaccgc aattgataac ggcgttagat 1860 gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt ttattatttt 1920 taagcgtgca taataagccc tacacaaatt gggagatata tcatgaaagg ctggcttttt 1980 cttgttatcg caatagttgg cgaagtaatc gcaacatccg cattaaaatc tagcgagggc 2040 tttactaagc ttgccccttc cgccgttgtc ataatcggtt atggcatcgc attttatttt 2100 ctttctctgg ttctgaaatc catccctgtc ggtgttgctt atgcagtctg gtcgggactc 2160 ggcgtcgtca taattacagc cattgcctgg ttgcttcatg ggcaaaagct tgatgcgtgg 2220 ggctttgtag gtatggggct cataattgct gcctttttgc tcgcccgatc cccatcgtgg 2280 aagtcgctgc ggaggccgac gccatggtga cggtgttcgg cattctgaat ctcaccgagg 2340 actccttctt cgatgagagc cggcggctag accccgccgg cgctgtcacc gcggcgatcg 2400 aaatgctgcg agtcggatca gacgtcgtgg atgtcggacc ggccgccagc catccggacg 2460 cgaggcctgt atcgccggcc gatgagatca gacgtattgc gccgctctta gacgccctgt 2520 ccgatcagat gcaccgtgtt tcaatcgaca gcttccaacc ggaaacccag cgctatgcgc 2580 tcaagcgcgg cgtgggctac ctgaacgata tccaaggatt tcctgaccct gcgctctatc 2640 ccgatattgc tgaggcggac tgcag 2665 // |
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